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Protein

Carbamoyl-phosphate synthase arginine-specific small chain

Gene

arg5

Organism
Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalytic activityi

2 ATP + L-glutamine + HCO3- + H2O = 2 ADP + phosphate + L-glutamate + carbamoyl phosphate.

Pathwayi: L-arginine biosynthesis

This protein is involved in step 1 of the subpathway that synthesizes carbamoyl phosphate from bicarbonate.
Proteins known to be involved in this subpathway in this organism are:
  1. Carbamoyl-phosphate synthase arginine-specific large chain (arg4), Carbamoyl-phosphate synthase arginine-specific small chain (arg5)
This subpathway is part of the pathway L-arginine biosynthesis, which is itself part of Amino-acid biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes carbamoyl phosphate from bicarbonate, the pathway L-arginine biosynthesis and in Amino-acid biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei301NucleophileBy similarity1
Active sitei385By similarity1
Active sitei387By similarity1

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Ligase

Keywords - Biological processi

Amino-acid biosynthesis, Arginine biosynthesis

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

UniPathwayiUPA00068; UER00171.

Names & Taxonomyi

Protein namesi
Recommended name:
Carbamoyl-phosphate synthase arginine-specific small chain (EC:6.3.5.5)
Short name:
CPS-A
Alternative name(s):
Arginine-specific carbamoyl-phosphate synthetase, glutamine chain
Gene namesi
Name:arg5
Synonyms:cpa1
ORF Names:SPBC56F2.09c
OrganismiSchizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Taxonomic identifieri284812 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaTaphrinomycotinaSchizosaccharomycetesSchizosaccharomycetalesSchizosaccharomycetaceaeSchizosaccharomyces
Proteomesi
  • UP000002485 Componenti: Chromosome II

Organism-specific databases

EuPathDBiFungiDB:SPBC56F2.09c.
PomBaseiSPBC56F2.09c. arg5.

Subcellular locationi

GO - Cellular componenti

  • carbamoyl-phosphate synthase complex Source: PomBase
  • cytoplasm Source: PomBase
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002905991 – 415Carbamoyl-phosphate synthase arginine-specific small chainAdd BLAST415

Proteomic databases

MaxQBiO60060.
PRIDEiO60060.

Interactioni

Subunit structurei

Composed of two chains; the small (or glutamine) chain promotes the hydrolysis of glutamine to ammonia, which is used by the large (or ammonia) chain to synthesize carbamoyl phosphate.By similarity

Protein-protein interaction databases

BioGridi277575. 3 interactors.
MINTiMINT-4676835.

Structurei

3D structure databases

ProteinModelPortaliO60060.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini225 – 412Glutamine amidotransferase type-1Add BLAST188

Sequence similaritiesi

Belongs to the CarA family.Curated

Keywords - Domaini

Glutamine amidotransferase

Phylogenomic databases

HOGENOMiHOG000038087.
InParanoidiO60060.
KOiK01956.
OMAiIEYKEYE.
OrthoDBiEOG092C20Y3.
PhylomeDBiO60060.

Family and domain databases

Gene3Di3.40.50.880. 1 hit.
3.50.30.20. 1 hit.
HAMAPiMF_01209. CPSase_S_chain. 1 hit.
InterProiIPR006274. CarbamoylP_synth_ssu.
IPR002474. CarbamoylP_synth_ssu_N.
IPR029062. Class_I_gatase-like.
IPR017926. GATASE.
[Graphical view]
PfamiPF00988. CPSase_sm_chain. 1 hit.
PF00117. GATase. 1 hit.
[Graphical view]
SMARTiSM01097. CPSase_sm_chain. 1 hit.
[Graphical view]
SUPFAMiSSF52021. SSF52021. 1 hit.
SSF52317. SSF52317. 1 hit.
TIGRFAMsiTIGR01368. CPSaseIIsmall. 1 hit.
PROSITEiPS51273. GATASE_TYPE_1. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

O60060-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MLRFLKPFPL RFGKRFYSKV PPVTNHERIL PKQPSFPTAP AQNEIATLTI
60 70 80 90 100
RNGPIFHGTS FGANRNVSGE AVFTTSPVGY VESLTDPSYK QQILIFTQPL
110 120 130 140 150
IGNYGVPDCK KRDENGLLRH FESPHIQCAG VVVNDYATKY SHWTAVESLG
160 170 180 190 200
EWCAREGVAA ITGVDTRAIV TFLREQGSSL AKISIGEEYD ANDDEAFINP
210 220 230 240 250
EEVNLVSQVS TREPFFVSGG DGMLNIAVID CGVKENILRS LVSRGASVTV
260 270 280 290 300
FPFDYPIQNV ASNYDGIFLT NGPGDPTHLT KTVNNLRELM NTYNGPIMGI
310 320 330 340 350
CMGHQLLALS TGAKTIKLKY GNRGHNIPAL DIASGNCHIT SQNHGYAVDA
360 370 380 390 400
STLPAEWKAT WTNLNDQSNE GIAHVSRPIS SVQFHPEARG GPMDTFYLFD
410
NYIKEAIKYQ KSRTA
Length:415
Mass (Da):45,662
Last modified:August 1, 1998 - v1
Checksum:i9D3AF94026943980
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CU329671 Genomic DNA. Translation: CAA18888.1.
PIRiT40535.
RefSeqiNP_596708.1. NM_001022633.2.

Genome annotation databases

EnsemblFungiiSPBC56F2.09c.1; SPBC56F2.09c.1:pep; SPBC56F2.09c.
GeneIDi2541060.
KEGGispo:SPBC56F2.09c.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CU329671 Genomic DNA. Translation: CAA18888.1.
PIRiT40535.
RefSeqiNP_596708.1. NM_001022633.2.

3D structure databases

ProteinModelPortaliO60060.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi277575. 3 interactors.
MINTiMINT-4676835.

Proteomic databases

MaxQBiO60060.
PRIDEiO60060.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiSPBC56F2.09c.1; SPBC56F2.09c.1:pep; SPBC56F2.09c.
GeneIDi2541060.
KEGGispo:SPBC56F2.09c.

Organism-specific databases

EuPathDBiFungiDB:SPBC56F2.09c.
PomBaseiSPBC56F2.09c. arg5.

Phylogenomic databases

HOGENOMiHOG000038087.
InParanoidiO60060.
KOiK01956.
OMAiIEYKEYE.
OrthoDBiEOG092C20Y3.
PhylomeDBiO60060.

Enzyme and pathway databases

UniPathwayiUPA00068; UER00171.

Miscellaneous databases

PROiO60060.

Family and domain databases

Gene3Di3.40.50.880. 1 hit.
3.50.30.20. 1 hit.
HAMAPiMF_01209. CPSase_S_chain. 1 hit.
InterProiIPR006274. CarbamoylP_synth_ssu.
IPR002474. CarbamoylP_synth_ssu_N.
IPR029062. Class_I_gatase-like.
IPR017926. GATASE.
[Graphical view]
PfamiPF00988. CPSase_sm_chain. 1 hit.
PF00117. GATase. 1 hit.
[Graphical view]
SMARTiSM01097. CPSase_sm_chain. 1 hit.
[Graphical view]
SUPFAMiSSF52021. SSF52021. 1 hit.
SSF52317. SSF52317. 1 hit.
TIGRFAMsiTIGR01368. CPSaseIIsmall. 1 hit.
PROSITEiPS51273. GATASE_TYPE_1. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiCARA_SCHPO
AccessioniPrimary (citable) accession number: O60060
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 12, 2007
Last sequence update: August 1, 1998
Last modified: November 30, 2016
This is version 107 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. Schizosaccharomyces pombe
    Schizosaccharomyces pombe: entries and gene names
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.