Reviewed, UniProtKB/Swiss-Prot O60052 (FNTA_SCHPO)

Names and origin

Protein names

Recommended name:
    Protein farnesyltransferase/geranylgeranyltransferase type-1 subunit alpha
    EC=2.5.1.58
    EC=2.5.1.59
Alternative name(s):
    CAAX farnesyltransferase subunit alpha
    Ras proteins prenyltransferase subunit alpha
    FTase-alpha
    Type I protein geranyl-geranyltransferase subunit alpha
      Short name=GGTase-I-alpha

Gene names

Name:	cwp1
ORF Names:	SPAPB1A10.04c

Organism

Schizosaccharomyces pombe (Fission yeast) [Complete proteome]

Taxonomic identifier

4896 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Taphrinomycotina › Schizosaccharomycetes › Schizosaccharomycetales › Schizosaccharomycetaceae › Schizosaccharomyces

Protein attributes

Sequence length	294 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Catalyzes the transfer of a farnesyl or geranyl-geranyl moiety from farnesyl or geranyl-geranyl pyrophosphate to a cysteine at the fourth position from the C-terminus of several proteins having the C-terminal sequence Cys-aliphatic-aliphatic-X. The alpha subunit is thought to participate in a stable complex with the substrate. The beta subunit binds the peptide substrate. Ref.1
Catalytic activity	Farnesyl diphosphate + protein-cysteine = S-farnesyl protein + diphosphate. Geranylgeranyl diphosphate + protein-cysteine = S-geranylgeranyl-protein + diphosphate.
Subunit structure	Heterodimer of an alpha and a beta subunit. Ref.1
Sequence similarities	Belongs to the protein prenyltransferase subunit alpha family. Contains 5 PFTA repeats.

Ontologies

Keywords
Domain	Repeat
Molecular function	Prenyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	1,3-beta-glucan biosynthetic process Ref.1 Inferred from physical interaction. Source: GeneDB_SPombe protein amino acid geranylgeranylation Inferred by curator. Source: GeneDB_SPombe
Cellular component	CAAX-protein geranylgeranyltransferase complex Non-traceable author statement. Source: GeneDB_SPombe nucleus Inferred from direct assay. Source: GeneDB_SPombe
Molecular function	CAAX-protein geranylgeranyltransferase activity Inferred from electronic annotation. Source: EC Rab geranylgeranyltransferase activity Ref.1 Inferred from physical interaction. Source: GeneDB_SPombe protein binding Inferred from physical interaction. Source: GeneDB_SPombe protein farnesyltransferase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 294	294	Protein farnesyltransferase/geranylgeranyltransferase type-1 subunit alpha		PRO_0000119753
Regions
	Repeat	57 – 91	35	PFTA 1
	Repeat	92 – 125	34	PFTA 2
	Repeat	126 – 160	35	PFTA 3
	Repeat	161 – 194	34	PFTA 4
	Repeat	199 – 233	35	PFTA 5

Sequences

	Sequence		Length	Mass (Da)
	O60052-1 [UniParc]. Last modified August 1, 1998. Version 1. Checksum: 840889770F11451E	FASTA	294	34,876
	10 20 30 40 50 60 MDPIDPELNE ILDFTEYGPL TPIPQDDGEN PLAKICYTTG YEQGMAYFRA IMAKKEYSLR 70 80 90 100 110 120 ALNLTGFLIM NNPAHYTVWA YRFQILNHTP SYIDNELEWL DEIAEDFQKN YQVWHHRQKI 130 140 150 160 170 180 LSLTKNYERE LEFTKKMFEI DSKNYHVWSY RVWILQNFND YSQELKLTNE LLEKDIYNNS 190 200 210 220 230 240 AWNHRFYVLF ETSKVVSWSL EEELNYLKDK ILFAPDNQSA WNYLCGVLDK SGPSKLDNLI 250 260 270 280 290 ANLRKNLPAL HKPLLEFLAM YEPSSSEEIY QKLANEVDVP HAALWTWMSQ RSNP

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Characterization of the geranylgeranyl transferase type I from Schizosaccharomyces pombe." Arellano M., Coll P.M., Yang W., Duran A., Tamanoi F., Perez P. Mol. Microbiol. 29:1357-1367(1998) [PubMed: 9781874] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION, SUBUNIT. Strain: ATCC 38366 / 972.
[2]	"The genome sequence of Schizosaccharomyces pombe." Wood V., Gwilliam R., Rajandream M.A., Lyne M.H., Lyne R., Stewart A., Sgouros J.G., Peat N., Hayles J., Baker S.G., Basham D., Bowman S., Brooks K., Brown D., Brown S., Chillingworth T., Churcher C.M., Collins M. , Connor R., Cronin A., Davis P., Feltwell T., Fraser A., Gentles S., Goble A., Hamlin N., Harris D.E., Hidalgo J., Hodgson G., Holroyd S., Hornsby T., Howarth S., Huckle E.J., Hunt S., Jagels K., James K.D., Jones L., Jones M., Leather S., McDonald S., McLean J., Mooney P., Moule S., Mungall K.L., Murphy L.D., Niblett D., Odell C., Oliver K., O'Neil S., Pearson D., Quail M.A., Rabbinowitsch E., Rutherford K.M., Rutter S., Saunders D., Seeger K., Sharp S., Skelton J., Simmonds M.N., Squares R., Squares S., Stevens K., Taylor K., Taylor R.G., Tivey A., Walsh S.V., Warren T., Whitehead S., Woodward J.R., Volckaert G., Aert R., Robben J., Grymonprez B., Weltjens I., Vanstreels E., Rieger M., Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Duesterhoeft A., Fritzc C., Holzer E., Moestl D., Hilbert H., Borzym K., Langer I., Beck A., Lehrach H., Reinhardt R., Pohl T.M., Eger P., Zimmermann W., Wedler H., Wambutt R., Purnelle B., Goffeau A., Cadieu E., Dreano S., Gloux S., Lelaure V., Mottier S., Galibert F., Aves S.J., Xiang Z., Hunt C., Moore K., Hurst S.M., Lucas M., Rochet M., Gaillardin C., Tallada V.A., Garzon A., Thode G., Daga R.R., Cruzado L., Jimenez J., Sanchez M., del Rey F., Benito J., Dominguez A., Revuelta J.L., Moreno S., Armstrong J., Forsburg S.L., Cerutti L., Lowe T., McCombie W.R., Paulsen I., Potashkin J., Shpakovski G.V., Ussery D., Barrell B.G., Nurse P. Nature 415:871-880(2002) [PubMed: 11859360] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 38366 / 972.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AJ223304 Genomic DNA. Translation: CAA11246.1. CU329670 Genomic DNA. Translation: CAC21477.1.
RefSeq	NP_593518.1.
3D structure databases
SMR	O60052. Positions 13-291.
ModBase	Search...
Protein-protein interaction databases
STRING	O60052.
Genome annotation databases
GeneID	2543143.
GenomeReviews	Gene locus cwp1 in contig CU329670_GR.
KEGG	spo:SPAPB1A10.04c.
NMPDR	fig|4896.1.peg.3488.
Organism-specific databases
GeneDB_Spombe	SPAPB1A10.04c.
Phylogenomic databases
eggNOG	fuNOG08714.
OMA	LENELCA.
OrthoDB	EOG9G7DG7.
PhylomeDB	O60052.
Enzyme and pathway databases
BRENDA	2.5.1.58. 653. 2.5.1.59. 653.
Gene expression databases
ArrayExpress	O60052.
Family and domain databases
InterPro	IPR002088. Prenyl_trans_a. IPR008940. Prenyltransferase. [Graphical view]
Gene3D	G3DSA:1.25.40.120. Prenyl_trans. 1 hit.
Pfam	PF01239. PPTA. 5 hits. [Graphical view]
PROSITE	PS51147. PFTA. 5 hits. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

FNTA_SCHPO

Accession

Primary (citable) accession number: O60052

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 6, 2002
Last sequence update:	August 1, 1998
Last modified:	January 19, 2010
This is version 58 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

FPAP (Fungal Proteome Annotation Project)