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Protein

Aspartic protease

Gene

pr1

Organism
Phaffia rhodozyma (Yeast) (Xanthophyllomyces dendrorhous)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Possesses acidic protease activity. Hydrolyzes casein and azoalbumin in vitro.1 Publication

Enzyme regulationi

Inhibited by pepstatin A.1 Publication

pH dependencei

Optimum pH is 4-6.1 Publication

Temperature dependencei

Optimum temperature is approximately 40 degrees Celsius.1 Publication

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei113 – 1131PROSITE-ProRule annotationBy similarity
Active sitei290 – 2901PROSITE-ProRule annotationBy similarity

GO - Molecular functioni

  • aspartic-type endopeptidase activity Source: UniProtKB

GO - Biological processi

  • proteolysis Source: UniProtKB
Complete GO annotation...

Keywords - Molecular functioni

Aspartyl protease, Hydrolase, Protease

Names & Taxonomyi

Protein namesi
Recommended name:
Aspartic protease1 PublicationImported (EC:3.4.23.-)
Gene namesi
Name:pr11 PublicationImported
OrganismiPhaffia rhodozyma (Yeast) (Xanthophyllomyces dendrorhous)
Taxonomic identifieri5421 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaBasidiomycotaAgaricomycotinaTremellomycetesCystofilobasidialesMrakiaceaeXanthophyllomyces

Subcellular locationi

  • Secreted 1 Publication

GO - Cellular componenti

  • extracellular region Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2121Sequence analysisAdd
BLAST
Propeptidei22 – 8160Removed in mature form1 PublicationPRO_0000390379Add
BLAST
Chaini82 – 405324Aspartic protease1 PublicationPRO_0000390380Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi126 ↔ 131By similarity
Disulfide bondi332 ↔ 366By similarity

Keywords - PTMi

Cleavage on pair of basic residues, Disulfide bond, Zymogen

Structurei

3D structure databases

ProteinModelPortaliO60020.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini97 – 402306Peptidase A1PROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Belongs to the peptidase A1 family.Sequence analysis
Contains 1 peptidase A1 domain.PROSITE-ProRule annotation

Keywords - Domaini

Signal

Family and domain databases

Gene3Di2.40.70.10. 2 hits.
InterProiIPR001461. Aspartic_peptidase_A1.
IPR001969. Aspartic_peptidase_AS.
IPR033121. PEPTIDASE_A1.
IPR021109. Peptidase_aspartic_dom.
[Graphical view]
PANTHERiPTHR13683. PTHR13683. 1 hit.
PfamiPF00026. Asp. 1 hit.
[Graphical view]
PRINTSiPR00792. PEPSIN.
SUPFAMiSSF50630. SSF50630. 1 hit.
PROSITEiPS00141. ASP_PROTEASE. 1 hit.
PS51767. PEPTIDASE_A1. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

O60020-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MISDTVIAIL AVALVGSTVQ AAPVDATATS TSGIIAVPIS KSAAQLAREA
60 70 80 90 100
DPVVSLDWLK KTKAQAQYKH KQANARLHSK RATGASVLTD QGSESLWTGP
110 120 130 140 150
ITIGGQSFTV DWDTGSSDLW VPSSACSSAA CNAHHKYTLT STGKKQSGTF
160 170 180 190 200
SISYGDGSSA SGPVYKDNVV ASGLQATSQV FGAVTSESSS FSSDPSDGIS
210 220 230 240 250
GLGWPALAQL SGTSYFWSLI NQGTVTSPVF SFRLATTNSE LYLGGINSAH
260 270 280 290 300
YTGAITYTPV TQKAYWTIAL GGVSVNGAAI NPSVSSAIID TGTTLVYGPT
310 320 330 340 350
AGVAALYAKI PGSASMADTY GSDYQGYYTF PCSAVPTVAL TFGGSSFSVP
360 370 380 390 400
TSAFNLGTVS SGSKQCVGGI VGQGDGSWLV GDVFLQGVYS IYDVGNARVG

FAKTV
Length:405
Mass (Da):41,370
Last modified:August 1, 1998 - v1
Checksum:iB722F5A28B6D7272
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF064871 mRNA. Translation: AAC17105.1.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF064871 mRNA. Translation: AAC17105.1.

3D structure databases

ProteinModelPortaliO60020.
ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Family and domain databases

Gene3Di2.40.70.10. 2 hits.
InterProiIPR001461. Aspartic_peptidase_A1.
IPR001969. Aspartic_peptidase_AS.
IPR033121. PEPTIDASE_A1.
IPR021109. Peptidase_aspartic_dom.
[Graphical view]
PANTHERiPTHR13683. PTHR13683. 1 hit.
PfamiPF00026. Asp. 1 hit.
[Graphical view]
PRINTSiPR00792. PEPSIN.
SUPFAMiSSF50630. SSF50630. 1 hit.
PROSITEiPS00141. ASP_PROTEASE. 1 hit.
PS51767. PEPTIDASE_A1. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Cloning and characterization of an endo-beta-1,3(4)glucanase and an aspartic protease from Phaffia rhodozyma CBS 6938."
    Bang M.L., Villadsen I., Sandal T.
    Appl. Microbiol. Biotechnol. 51:215-222(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], PROTEIN SEQUENCE OF 82-92, FUNCTION, ENZYME REGULATION, BIOPHYSICOCHEMICAL PROPERTIES, SUBCELLULAR LOCATION.
    Strain: CBS 69381 Publication.

Entry informationi

Entry nameiASPR1_PHARH
AccessioniPrimary (citable) accession number: O60020
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 15, 2009
Last sequence update: August 1, 1998
Last modified: July 6, 2016
This is version 68 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Direct protein sequencing

Documents

  1. Peptidase families
    Classification of peptidase families and list of entries
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.