O60017 (DAK_PICAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Dihydroxyacetone kinase
Short name=DHA kinase
EC=2.7.1.29

Alternative name(s):
Glycerone kinase

Gene names

Name:

DAK

Organism

Pichia angusta (Yeast) (Hansenula polymorpha)

Taxonomic identifier

870730 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetales incertae sedis › Ogataea

Protein attributes

Sequence length	609 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	ATP + glycerone = ADP + glycerone phosphate.
Pathway	Polyol metabolism; glycerol fermentation; glycerone phosphate from glycerol (oxidative route): step 2/2.
Sequence similarities	Belongs to the dihydroxyacetone kinase (DAK) family. Contains 1 DhaK domain. Contains 1 DhaL domain.

Ontologies

Keywords
Biological process	Glycerol metabolism
Ligand	ATP-binding Nucleotide-binding
Molecular function	Kinase Transferase
Gene Ontology (GO)
Biological process	glycerol metabolic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW glycerone kinase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 609	609	Dihydroxyacetone kinase		PRO_0000121518
Regions
	Domain	8 – 355	348	DhaK
	Domain	392 – 600	209	DhaL
	Nucleotide binding	421 – 424	4	ATP By similarity
	Nucleotide binding	467 – 468	2	ATP By similarity
	Nucleotide binding	523 – 524	2	ATP By similarity
	Nucleotide binding	585 – 587	3	ATP By similarity
	Region	53 – 56	4	Substrate binding By similarity
Sites
	Active site	232	1	Tele-hemiaminal-histidine intermediate By similarity
	Binding site	104	1	Substrate By similarity
	Binding site	109	1	Substrate By similarity

Sequences

	Sequence		Length	Mass (Da)
	O60017 [UniParc]. Last modified August 1, 1998. Version 1. Checksum: 16A0CF4439C967AA	FASTA	609	65,071
	10 20 30 40 50 60 MSSKHWNYKQ DLVHAHLKGL CHANPDLQFI ESERVVINKH SKPDKVMILS GGGSGHEPLH 70 80 90 100 110 120 AGFVGEGCLD VGVAGFVFAS PSTKQIVSGL KAKPSDKGTL IVVKNYTGDI LHFGLAAERA 130 140 150 160 170 180 KAEGVPVELL IVQDDVSVGR TKNGMVGRRG LAGTSLVHKI VGAKAAKDSN KASLSEVYQL 190 200 210 220 230 240 GEAVVANLVT IGASLDHCTI PGNRHHESES DDEDEQKHLL KEDEIEVGMG IHNESGIKRV 250 260 270 280 290 300 SPIPTIDTLV ADLLKYLLDK SDEERHYVDF DSSDEVVLMI NNLGGTSNLE LYAIQNTVVE 310 320 330 340 350 360 QLATDYKIKP ARVYTGAYTT SLDGPGFSIT LLNVTRAGGK EVFDCLDYPT KVPGWNSSYT 370 380 390 400 410 420 TAEWAAKSES FVIDAPPVSD ASATSKVRFS SSTVKAVLES GCKKLLTKEP KITLYDTVAG 430 440 450 460 470 480 DGDCGETLAN GAHAILDLLA ADKLEITDGV RSLTQITDVV ETAMGGTSGG LYSIFISALA 490 500 510 520 530 540 KSLKDRELQQ GGYEVTPQIL AASLKDALES LYRYTRARAG DRTLIDALAP FVEQFAASKG 550 560 570 580 590 600 DLNQANKACH EGAESTRKLK AKFGRASYVS EEEFKPFEAE GGLPDPGAIG LAALVDGFAE AYSKIGSNL

References

[1]

"The Hansenula polymorpha per6 mutant is affected in two adjacent genes which encode dihydroxyacetone kinase and a novel protein, Pak1p, involved in peroxisome integrity." van der Klei I.J., van der Heide M., Baerends R.J.S., Rechinger K.-B., Nicolay K., Kiel J.A.K.W., Veenhuis M. Curr. Genet. 34:1-11(1998) [PubMed: 9683670] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 34438 / CBS 4732 / DSM 70277 / JCM 3621 / NBRC 1476 / NRRL Y-5445.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AY383554 Genomic DNA. Translation: AAC27705.1.
3D structure databases
ProteinModelPortal	O60017.
ModBase	Search...
Proteomic databases
PRIDE	O60017.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Enzyme and pathway databases
BioCyc	MetaCyc:MONOMER-13163.
Family and domain databases
InterPro	IPR004006. Dak1. IPR004007. Dak2. IPR012734. DhaK_ATP. [Graphical view]
Pfam	PF02733. Dak1. 1 hit. PF02734. Dak2. 1 hit. [Graphical view]
SUPFAM	SSF101473. Dak2. 1 hit.
TIGRFAMs	TIGR02361. Dak_ATP. 1 hit.
PROSITE	PS51481. DHAK. 1 hit. PS51480. DHAL. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

DAK_PICAN

Accession

Primary (citable) accession number: O60017

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 30, 2000
Last sequence update:	August 1, 1998
Last modified:	December 14, 2011
This is version 53 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program