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O60015 (UBCX_PICAN) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 73. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Ubiquitin-conjugating enzyme E2-21 kDa

EC=6.3.2.19
Alternative name(s):
Peroxin-4
Ubiquitin carrier protein
Ubiquitin-protein ligase
Gene names
Name:PEX4
OrganismPichia angusta (Yeast) (Hansenula polymorpha)
Taxonomic identifier870730 [NCBI]
Taxonomic lineageEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetales incertae sedisOgataea

Protein attributes

Sequence length188 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Catalyzes the covalent attachment of ubiquitin to other proteins. Essential for peroxisome biogenesis. Required for UBC4-independent ubiquitination of PEX5 By similarity.

Catalytic activity

ATP + ubiquitin + protein lysine = AMP + diphosphate + protein N-ubiquityllysine.

Pathway

Protein modification; protein ubiquitination.

Sequence similarities

Belongs to the ubiquitin-conjugating enzyme family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 188188Ubiquitin-conjugating enzyme E2-21 kDa
PRO_0000082557

Sites

Active site1191Glycyl thioester intermediate By similarity

Sequences

Sequence LengthMass (Da)Tools
O60015 [UniParc].

Last modified August 1, 1998. Version 1.
Checksum: 0BC293A5E0787CB8

FASTA18821,531
        10         20         30         40         50         60 
MSSTEKRLLK EYRAVKKELT EKRSPIHDTG IVDLHPLEDG LFRWSAVIRG PDQSPFEDAL 

        70         80         90        100        110        120 
WKLEIDIPTN YPLDPPKIKF VVFGEEKIRQ LQRKTSSGAR KVCYKMPHPN VNFKTGEICL 

       130        140        150        160        170        180 
DILQQKWSPA WTLQSALVAI VVLLANPEPL SPLNIDMANL LKCDDTTAYK DLVHYYIAKY 


SAYESNDV 

« Hide

References

[1]"The ubiquitin-conjugating enzyme Pex4p of Hansenula polymorpha is required for efficient functioning of the PTS1 import machinery."
van der Klei I.J., Hilbrands R.E., Kiel J.A.K.W., Rasmussen S.W., Cregg J.M., Veenhuis M.
EMBO J. 17:3608-3618(1998) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: ATCC 34438 / CBS 4732 / DSM 70277 / JCM 3621 / NBRC 1476 / NRRL Y-5445.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AF061604 Genomic DNA. Translation: AAC16238.1.

3D structure databases

ProteinModelPortalO60015.
ModBaseSearch...
MobiDBSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Enzyme and pathway databases

UniPathwayUPA00143.

Family and domain databases

Gene3D3.10.110.10. 1 hit.
InterProIPR000608. UBQ-conjugat_E2.
IPR023313. UBQ-conjugating_AS.
IPR016135. UBQ-conjugating_enzyme/RWD.
[Graphical view]
PfamPF00179. UQ_con. 1 hit.
[Graphical view]
SUPFAMSSF54495. SSF54495. 1 hit.
PROSITEPS00183. UBIQUITIN_CONJUGAT_1. 1 hit.
PS50127. UBIQUITIN_CONJUGAT_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameUBCX_PICAN
AccessionPrimary (citable) accession number: O60015
Entry history
Integrated into UniProtKB/Swiss-Prot: December 15, 1998
Last sequence update: August 1, 1998
Last modified: April 16, 2014
This is version 73 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways