O60015 (UBCX_PICAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Ubiquitin-conjugating enzyme E2-21 kDa
EC=6.3.2.19

Alternative name(s):
Peroxin-4
Ubiquitin carrier protein
Ubiquitin-protein ligase

Gene names

Name:

PEX4

Organism

Pichia angusta (Yeast) (Hansenula polymorpha)

Taxonomic identifier

870730 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Fungi › Dikarya › Ascomycota › saccharomyceta › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetales incertae sedis › Ogataea

Protein attributes

Sequence length	188 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the covalent attachment of ubiquitin to other proteins. Essential for peroxisome biogenesis. Required for UBC4-independent ubiquitination of PEX5 By similarity.
Catalytic activity	ATP + ubiquitin + protein lysine = AMP + diphosphate + protein N-ubiquityllysine.
Pathway	Protein modification; protein ubiquitination.
Sequence similarities	Belongs to the ubiquitin-conjugating enzyme family.

Ontologies

Keywords
Biological process	Peroxisome biogenesis Ubl conjugation pathway
Ligand	ATP-binding Nucleotide-binding
Molecular function	Ligase
Gene Ontology (GO)
Biological_process	peroxisome organization Inferred from electronic annotation. Source: UniProtKB-KW
Molecular_function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW ubiquitin-protein ligase activity Inferred from sequence or structural similarity. Source: UniProtKB
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 188	188	Ubiquitin-conjugating enzyme E2-21 kDa		PRO_0000082557
Sites
	Active site	119	1	Glycyl thioester intermediate By similarity

Sequences

	Sequence		Length	Mass (Da)
	O60015 [UniParc]. Last modified August 1, 1998. Version 1. Checksum: 0BC293A5E0787CB8	FASTA	188	21,531
	10 20 30 40 50 60 MSSTEKRLLK EYRAVKKELT EKRSPIHDTG IVDLHPLEDG LFRWSAVIRG PDQSPFEDAL 70 80 90 100 110 120 WKLEIDIPTN YPLDPPKIKF VVFGEEKIRQ LQRKTSSGAR KVCYKMPHPN VNFKTGEICL 130 140 150 160 170 180 DILQQKWSPA WTLQSALVAI VVLLANPEPL SPLNIDMANL LKCDDTTAYK DLVHYYIAKY SAYESNDV

References

[1]

"The ubiquitin-conjugating enzyme Pex4p of Hansenula polymorpha is required for efficient functioning of the PTS1 import machinery." van der Klei I.J., Hilbrands R.E., Kiel J.A.K.W., Rasmussen S.W., Cregg J.M., Veenhuis M. EMBO J. 17:3608-3618(1998) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 34438 / CBS 4732 / DSM 70277 / JCM 3621 / NBRC 1476 / NRRL Y-5445.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF061604 Genomic DNA. Translation: AAC16238.1.
3D structure databases
ProteinModelPortal	O60015.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Enzyme and pathway databases
UniPathway	UPA00143.
Family and domain databases
Gene3D	3.10.110.10. 1 hit.
InterPro	IPR000608. UBQ-conjugat_E2. IPR023313. UBQ-conjugating_AS. IPR016135. UBQ-conjugating_enzyme/RWD. [Graphical view]
Pfam	PF00179. UQ_con. 1 hit. [Graphical view]
SUPFAM	SSF54495. UBQ-conjugat/RWD-like. 1 hit.
PROSITE	PS00183. UBIQUITIN_CONJUGAT_1. 1 hit. PS50127. UBIQUITIN_CONJUGAT_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

UBCX_PICAN

Accession

Primary (citable) accession number: O60015

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 15, 1998
Last sequence update:	August 1, 1998
Last modified:	April 3, 2013
This is version 71 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program