Reviewed, UniProtKB/Swiss-Prot O59939 (PELB_COLGL)

Names and origin

Protein names

Recommended name:
    Pectate lyase B
    EC=4.2.2.2

Gene names

Name:

PLB

Organism

Colletotrichum gloeosporioides (Anthracnose fungus) (Glomerella cingulata)

Taxonomic identifier

474922 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Pezizomycotina › Sordariomycetes › Hypocreomycetidae › Hypocreomycetidae incertae sedis › Glomerellaceae › Glomerella

Protein attributes

Sequence length	331 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Acts as a virulence factor active in plant tissue maceration.
Catalytic activity	Eliminative cleavage of (1->4)-alpha-D-galacturonan to give oligosaccharides with 4-deoxy-alpha-D-galact-4-enuronosyl groups at their non-reducing ends.
Cofactor	Binds 1 calcium ion per subunit By similarity.
Pathway	Glycan metabolism; pectin degradation; 2-dehydro-3-deoxy-D-gluconate from pectin: step 2/5.
Subcellular location	Secreted Potential.
Sequence similarities	Belongs to the polysaccharide lyase 1 family.

Ontologies

Keywords
Biological process	Virulence
Cellular component	Secreted
Domain	Signal
Ligand	Calcium Metal-binding
Molecular function	Lyase
PTM	Glycoprotein
Gene Ontology (GO)
Biological process	pathogenesis Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	extracellular region Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	calcium ion binding Inferred from electronic annotation. Source: UniProtKB-KW pectate lyase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 25	25	Potential
	Chain	26 – 331	306	Pectate lyase B		PRO_0000024895
Sites
	Active site	226	1	Potential
	Metal binding	139	1	Calcium By similarity
	Metal binding	169	1	Calcium By similarity
	Metal binding	173	1	Calcium By similarity
Amino acid modifications
	Glycosylation	99	1	N-linked (GlcNAc...) Potential

Sequences

	Sequence		Length	Mass (Da)
	O59939-1 [UniParc]. Last modified August 1, 1998. Version 1. Checksum: 475A76F2525D4689	FASTA	331	35,108
	10 20 30 40 50 60 MKFTGSPLLW PSWLPLPAPP PPLPSVTRRR DPRTVGKRAA ITDACDVGYG AGTTGGSGGT 70 80 90 100 110 120 TTTVSTPAQF TAAATSDEKA VIVVKGAITG ATKVKVGSNK SIIGRAGSSL TGVGLYINKQ 130 140 150 160 170 180 ENVIVRNMKI SKVLADNGDR IGIQASSKVW VDHCDLSSDK KNNGKDYYDG LLDITHASMA 190 200 210 220 230 240 VTVSNTYIHD HYKGSLVGHS DSNSAEDTGK LYVTYANNHW YNVASRNPSV RFGNVHIFNN 250 260 270 280 290 300 YAEKLETSGV NTRMGAQLLI ESSVFSDTKK AVTFLDSKST GYAVVNDVDL GGSTNDRPQG 310 320 330 TFTKPDYSYT LLGSSKVKAA VVGTAGQTLT F

References

[1]	Wattad C., Keen N.T. Submitted (MAR-1998) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: CG-14.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AF052632 Genomic DNA. Translation: AAD09857.1.
3D structure databases
HSSP	HSSP built from PDB template 1JTA based on UniProtKB P29155.
ModBase	Search...
Protein family/group databases
CAZy	PL1. Polysaccharide Lyase Family 1.
Enzyme and pathway databases
BRENDA	4.2.2.2. 39832.
Family and domain databases
InterPro	IPR002022. Amb_allergen. IPR012334. Pectin_lyas_fold. [Graphical view]
Gene3D	G3DSA:2.160.20.10. Pectin_lyas_fold. 1 hit.
Pfam	PF00544. Pec_lyase_C. 1 hit. [Graphical view]
SMART	SM00656. Amb_all. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PELB_COLGL

Accession

Primary (citable) accession number: O59939

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 15, 1998
Last sequence update:	August 1, 1998
Last modified:	November 3, 2009
This is version 57 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

FPAP (Fungal Proteome Annotation Project)