Reviewed,
UniProtKB/Swiss-Prot O59933 (ERG25_CANAL)
Last modified
November 4, 2008.
Version 48.
History...
Clusters with 100%,
90%,
50% identity |
Documents (3) |
Third-party data |
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Names and origin
| Protein names | Recommended name: C-4 methylsterol oxidase EC=1.14.13.72 Alternative name(s): Methylsterol monooxygenase | ||
| Gene names |
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| Organism | Candida albicans (Yeast) | ||
| Taxonomic identifier | 5476 [NCBI] | ||
| Taxonomic lineage | Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › mitosporic Saccharomycetales › Candida |
Protein attributes
| Sequence length | 308 AA. |
| Sequence status | Complete. |
| Sequence processing | The displayed sequence is not processed. |
| Protein existence | Inferred from homology. |
General annotation (Comments)
| Function | Catalyzes the first step in the removal of the two C-4 methyl groups of 4,4-dimethylzymosterol By similarity. |
| Catalytic activity | 4,4-dimethyl-5-alpha-cholest-7-en-3-beta-ol + NAD(P)H + O(2) = 4-beta-hydroxymethyl-4-alpha-methyl-5-alpha-cholest-7-en-3-beta-ol + NAD(P)(+) + H(2)O. 4-beta-hydroxymethyl-4-alpha-methyl-5-alpha-cholest-7-en-3-beta-ol + NAD(P)H + O(2) = 3-beta-hydroxy-4-beta-methyl-5-alpha-cholest-7-ene-4-alpha-carbaldehyde + NAD(P)(+) + 2 H(2)O. 3-beta-hydroxy-4-beta-methyl-5-alpha-cholest-7-ene-4-alpha-carbaldehyde + NAD(P)H + O(2) = 3-beta-hydroxy-4-beta-methyl-5-alpha-cholest-7-ene-4-alpha-carboxylate + NAD(P)(+) + H(2)O. |
| Cofactor | Iron By similarity. |
| Pathway | Steroid biosynthesis; zymosterol biosynthesis; zymosterol from lanosterol: step 3/6. |
| Subcellular location | Endoplasmic reticulum membrane; Single-pass membrane proteinProbable. |
| Domain | The histidine box domains may contain the active site and/or be involved in metal ion binding. |
| Sequence similarities | Belongs to the sterol desaturase family. |
Ontologies
Keywords | |
|---|---|
| Biological process | Lipid synthesis Steroid biosynthesis Sterol biosynthesis |
| Cellular component | Endoplasmic reticulum Membrane |
| Domain | Transmembrane |
| Ligand | Iron NAD |
| Molecular function | Oxidoreductase |
Gene Ontology (GO) | |
| Biological process | oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW sterol biosynthetic processInferred from electronic annotation. Source: UniProtKB-KW |
| Cellular component | endoplasmic reticulum membrane Inferred from electronic annotation. Source: UniProtKB-SubCell integral to membraneInferred from electronic annotation. Source: UniProtKB-KW |
| Molecular function | C-4 methylsterol oxidase activity Inferred from electronic annotation. Source: EC iron ion bindingInferred from electronic annotation. Source: UniProtKB-KW |
| Complete GO annotation... | |
Sequence annotation (Features)
Sequences
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References
| [1] | "Cloning and sequencing of the Candida albicans C-4 sterol methyl oxidase gene (ERG25) and expression of an ERG25 conditional lethal mutation in Saccharomyces cerevisiae." Kennedy M.A., Johnson T.A., Lees N.D., Barbuch R., Eckstein J.A., Bard M. Lipids 35:257-262(2000) [PubMed: 10783002] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: CAI-8. |
Cross-references
Sequence databases | |
|---|---|
| AF051914 Genomic DNA. Translation: AAC06014.1. | |
3D structure databases | |
| ModBase | Search... |
Organism-specific databases | |
| CGD | CAL0001165. ERG25. |
Family and domain databases | |
| InterPro | IPR006088. Sterol_desatur. [Graphical view] |
| Pfam | PF01598. Sterol_desat. 1 hit. [Graphical view] |
| ProtoNet | Search... |
Entry information
| Entry name | ERG25_CANAL | ||||||||
| Accession | Primary (citable) accession number: O59933 | ||||||||
| Entry history |
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| Entry status | Reviewed (UniProtKB/Swiss-Prot) | ||||||||
| Annotation project | FPAP (Fungal Proteome Annotation Project) | ||||||||
Relevant documents
| Candida albicans Candida albicans: entries and gene names |
| PATHWAY comments Index of metabolic and biosynthesis pathways |
| SIMILARITY comments Index of protein domains and families |

Clusters with


