Reviewed, UniProtKB/Swiss-Prot O59925 (PGLR_PENEN)

Names and origin

Protein names

Recommended name:
    Polygalacturonase
      Short name=PG
    EC=3.2.1.15
Alternative name(s):
    Pectinase

Gene names

Name:

PEPG1

Organism

Penicillium expansum

Taxonomic identifier

27334 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Pezizomycotina › Eurotiomycetes › Eurotiomycetidae › Eurotiales › Trichocomaceae › mitosporic Trichocomaceae › Penicillium

Protein attributes

Sequence length	378 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	Random hydrolysis of (1->4)-alpha-D-galactosiduronic linkages in pectate and other galacturonans.
Subcellular location	Secreted Probable.
Sequence similarities	Belongs to the glycosyl hydrolase 28 family. Contains 5 PbH1 repeats.

Ontologies

Keywords
Biological process	Cell wall biogenesis/degradation
Cellular component	Secreted
Domain	Repeat Signal
Molecular function	Glycosidase Hydrolase
Gene Ontology (GO)
Biological process	carbohydrate metabolic process Inferred from electronic annotation. Source: InterPro cell wall organization Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	extracellular region Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	polygalacturonase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 20	20	Potential
	Chain	21 – 378	358	Polygalacturonase		PRO_0000024788
Sites
	Active site	218	1	Proton donor By similarity
	Active site	240	1	By similarity

Sequences

	Sequence		Length	Mass (Da)
	O59925-1 [UniParc]. Last modified August 1, 1998. Version 1. Checksum: 288518CAF7F6BBD0	FASTA	378	38,028
	10 20 30 40 50 60 MILTRSVVLG FLGSASLALA SPVAELAEGS RLTPRGSACS YSGTSGAAAA IAGKAGCSSI 70 80 90 100 110 120 TLNNVVVPAG TTLDLTGLAS GTKVIFEGTT TFGYKQWAGP LISISGTNIQ VSGASGHLID 130 140 150 160 170 180 GQGSRWWDGE GSNSKTNIKP KFFFAHSLKG SSTITGLNIK DSPVQVFSIS GSSGLTISGV 190 200 210 220 230 240 TIDNKNGDTN SLGHNTDGFD IGDSDSITIT GATVYNQDDC LAINSGTNIV FSGGYCSGGH 250 260 270 280 290 300 GLSIGSVGGR SNNVVETVHI SSTQVVNSQN GVRVKAVSGA TGTIKGVTFQ DITLSGITSQ 310 320 330 340 350 360 GITIRQEYTN SGYTGSPTTG VPITGLTLNN VHGTVTSKGT DITIECGSSA SCSGWTWTKV 370 AVSGGKADVC KNAPSGTC

References

[1]	"Cloning and analysis of a gene encoding polygalacturonase in Penicillium expansum." Yao C., Conway W.S., Ren R., Sams C.E. Submitted (FEB-1998) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	AF047713 Genomic DNA. Translation: AAC05492.1.
3D structure databases
HSSP	HSSP built from PDB template 1IB4 based on UniProtKB O74213.
ModBase	Search...
Protein family/group databases
CAZy	GH28. Glycoside Hydrolase Family 28.
Enzyme and pathway databases
BRENDA	3.2.1.15. 18850.
Family and domain databases
InterPro	IPR000743. Glyco_hydro_28. IPR006626. PbH1. IPR012334. Pectin_lyas_fold. [Graphical view]
Gene3D	G3DSA:2.160.20.10. Pectin_lyas_fold. 1 hit.
Pfam	PF00295. Glyco_hydro_28. 1 hit. [Graphical view]
SMART	SM00710. PbH1. 5 hits. [Graphical view]
PROSITE	PS00502. POLYGALACTURONASE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PGLR_PENEN

Accession

Primary (citable) accession number: O59925

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 30, 2000
Last sequence update:	August 1, 1998
Last modified:	June 16, 2009
This is version 46 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

FPAP (Fungal Proteome Annotation Project)