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O59896

- LAC1_PYCCI

UniProt

O59896 - LAC1_PYCCI

Protein

Laccase

Gene

LCC3-1

Organism
Pycnoporus cinnabarinus (Cinnabar-red polypore)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 72 (01 Oct 2014)
      Sequence version 1 (01 Aug 1998)
      Previous versions | rss
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    Functioni

    Most probably plays an important role in lignin degradation. Cleaves the C-C and C-O bonds of some phenolic lignin model compounds (such as O- and P-quinols, aminophenols and phenylenediamine). May also be involved in synthesis of phenoxazinone pigments.

    Catalytic activityi

    4 benzenediol + O2 = 4 benzosemiquinone + 2 H2O.

    Cofactori

    Binds 4 copper ions per monomer.By similarity

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi85 – 851Copper 1; type 2By similarity
    Metal bindingi87 – 871Copper 2; type 3By similarity
    Metal bindingi130 – 1301Copper 2; type 3By similarity
    Metal bindingi132 – 1321Copper 3; type 3By similarity
    Metal bindingi416 – 4161Copper 4; type 1By similarity
    Metal bindingi419 – 4191Copper 1; type 2By similarity
    Metal bindingi421 – 4211Copper 3; type 3By similarity
    Metal bindingi471 – 4711Copper 3; type 3By similarity
    Metal bindingi472 – 4721Copper 4; type 1By similarity
    Metal bindingi473 – 4731Copper 2; type 3By similarity
    Metal bindingi477 – 4771Copper 4; type 1By similarity

    GO - Molecular functioni

    1. copper ion binding Source: InterPro
    2. hydroquinone:oxygen oxidoreductase activity Source: UniProtKB-EC

    GO - Biological processi

    1. lignin catabolic process Source: UniProtKB-KW

    Keywords - Molecular functioni

    Oxidoreductase

    Keywords - Biological processi

    Lignin degradation

    Keywords - Ligandi

    Copper, Metal-binding

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Laccase (EC:1.10.3.2)
    Alternative name(s):
    Benzenediol:oxygen oxidoreductase
    Diphenol oxidase
    Ligninolytic phenoloxidase
    Urishiol oxidase
    Gene namesi
    Name:LCC3-1
    Synonyms:LAC3, LCC1
    OrganismiPycnoporus cinnabarinus (Cinnabar-red polypore)
    Taxonomic identifieri5643 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaBasidiomycotaAgaricomycotinaAgaricomycetesPolyporalesTrametes

    Subcellular locationi

    GO - Cellular componenti

    1. extracellular region Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Secreted

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Signal peptidei1 – 21211 PublicationAdd
    BLAST
    Chaini22 – 518497LaccasePRO_0000002934Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Glycosylationi72 – 721N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi75 – 751N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi229 – 2291N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi354 – 3541N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi362 – 3621N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi398 – 3981N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi455 – 4551N-linked (GlcNAc...)Sequence Analysis

    Keywords - PTMi

    Glycoprotein

    Expressioni

    Inductioni

    By lignosulfonate, veratryl alcohol and 2,5-xylidine.1 Publication

    Structurei

    3D structure databases

    ProteinModelPortaliO59896.
    SMRiO59896. Positions 22-517.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini23 – 148126Plastocyanin-like 1Add
    BLAST
    Domaini160 – 302143Plastocyanin-like 2Add
    BLAST
    Domaini369 – 489121Plastocyanin-like 3Add
    BLAST

    Sequence similaritiesi

    Belongs to the multicopper oxidase family.Curated
    Contains 3 plastocyanin-like domains.Curated

    Keywords - Domaini

    Repeat, Signal

    Family and domain databases

    Gene3Di2.60.40.420. 3 hits.
    InterProiIPR001117. Cu-oxidase.
    IPR011706. Cu-oxidase_2.
    IPR011707. Cu-oxidase_3.
    IPR002355. Cu_oxidase_Cu_BS.
    IPR008972. Cupredoxin.
    [Graphical view]
    PfamiPF00394. Cu-oxidase. 1 hit.
    PF07731. Cu-oxidase_2. 1 hit.
    PF07732. Cu-oxidase_3. 1 hit.
    [Graphical view]
    SUPFAMiSSF49503. SSF49503. 3 hits.
    PROSITEiPS00079. MULTICOPPER_OXIDASE1. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    O59896-1 [UniParc]FASTAAdd to Basket

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    MSRFQSLLSF VLVSLAAVAN AAIGPVADLT LTNAAVSPDG FSREAVVVNG    50
    ITPAPLIAGQ KGDRFQLNVI DNLTNHTMLK TTSIHWHGFF QHGTNWADGV 100
    SFVNQCPIAS GHSFLYDFQV PDQAGTFWYH SHLSTQYCDG LRGPFVVYDP 150
    NDPQASLYDI DNDDTVITLA DWYHVAAKLG PRFPLGADAT LINGLGRSPG 200
    TTTADLAVIK VTQGKRYRFR LVSLSCDPNH TFSIDGHTMT VIEADSVNTQ 250
    PLEVDSIQIF AAQRYSFVLD ASQPVDNYWI RANPAFGNVG FAGGINSAIL 300
    RYDGAPEVEP TTTQTTSTKP LNEADLHPLT PMPVPGRPEA GGVDKPLNMV 350
    FNFNGTNFFI NNHSFVPPSV PVLLQILSGA QAAQDLVPDG SVYVLPSNSS 400
    IEISFPATAN APGTPHPFHL HGHTFAVVRS AGSSEYNYDN PIFRDVVSTG 450
    QPGDNVTIRF QTNNPGPWFL HCHIDFHLEA GFAVVLAEDT PDTAAVNPVP 500
    QSWSDLCPIY DALDPSDL 518
    Length:518
    Mass (Da):55,986
    Last modified:August 1, 1998 - v1
    Checksum:i31B4386AFDB8DF9D
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti348 – 3481N → S in AAN71597. 1 PublicationCurated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF025481 Genomic DNA. Translation: AAC39469.1.
    AY147188 Genomic DNA. Translation: AAN71597.1.
    AJ420334 mRNA. Translation: CAD12461.1.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF025481 Genomic DNA. Translation: AAC39469.1 .
    AY147188 Genomic DNA. Translation: AAN71597.1 .
    AJ420334 mRNA. Translation: CAD12461.1 .

    3D structure databases

    ProteinModelPortali O59896.
    SMRi O59896. Positions 22-517.
    ModBasei Search...
    MobiDBi Search...

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Family and domain databases

    Gene3Di 2.60.40.420. 3 hits.
    InterProi IPR001117. Cu-oxidase.
    IPR011706. Cu-oxidase_2.
    IPR011707. Cu-oxidase_3.
    IPR002355. Cu_oxidase_Cu_BS.
    IPR008972. Cupredoxin.
    [Graphical view ]
    Pfami PF00394. Cu-oxidase. 1 hit.
    PF07731. Cu-oxidase_2. 1 hit.
    PF07732. Cu-oxidase_3. 1 hit.
    [Graphical view ]
    SUPFAMi SSF49503. SSF49503. 3 hits.
    PROSITEi PS00079. MULTICOPPER_OXIDASE1. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Molecular analysis of a laccase gene from the white rot fungus Pycnoporus cinnabarinus."
      Eggert C., LaFayette P.R., Temp U., Eriksson K.-E.L., Dean J.F.D.
      Appl. Environ. Microbiol. 64:1766-1772(1998) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: ATCC 200478 / PB.
      Tissue: Mycelium.
    2. "Cloning, characterization and expression of laccase gene from white rot fungus Pycnoporus cinnabarinus."
      Dhawan S., Lal R., Kuhad R.C.
      Submitted (SEP-2002) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    3. "Molecular biological monitoring of soil fungi with laccase genes."
      Luis P., Buscot F.
      Submitted (NOV-2001) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE OF 85-131.
      Tissue: Mycelium.
    4. "The ligninolytic system of the white rot fungus Pycnoporus cinnabarinus: purification and characterization of the laccase."
      Eggert C., Temp U., Eriksson K.-E.L.
      Appl. Environ. Microbiol. 62:1151-1158(1996) [PubMed] [Europe PMC] [Abstract]
      Cited for: PROTEIN SEQUENCE OF 22-42, CHARACTERIZATION, INDUCTION.
      Strain: ATCC 200478 / PB.
      Tissue: Mycelium.

    Entry informationi

    Entry nameiLAC1_PYCCI
    AccessioniPrimary (citable) accession number: O59896
    Secondary accession number(s): Q8J1Y2, Q8WZN9
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: September 19, 2003
    Last sequence update: August 1, 1998
    Last modified: October 1, 2014
    This is version 72 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Direct protein sequencing

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3