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O59863

- PLB_KLULA

UniProt

O59863 - PLB_KLULA

Protein

Lysophospholipase

Gene

PLB

Organism
Kluyveromyces lactis (strain ATCC 8585 / CBS 2359 / DSM 70799 / NBRC 1267 / NRRL Y-1140 / WM37) (Yeast) (Candida sphaerica)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 80 (01 Oct 2014)
      Sequence version 1 (01 Aug 1998)
      Previous versions | rss
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    Functioni

    Catalyzes the release of fatty acids from lysophospholipids. At acidic pH the enzyme hydrolyzes all phospholipid substrates without metal ion. On the other hand, at alkaline pH the enzyme shows substrate specificity for phosphatidylcholine and lysophosphatidylcholine and requires Ca2+, Fe3+, or Al3+ for the activity.

    Catalytic activityi

    2-lysophosphatidylcholine + H2O = glycerophosphocholine + a carboxylate.

    pH dependencei

    Optimum pH is 2.0 and 7.5.

    GO - Molecular functioni

    1. lysophospholipase activity Source: UniProtKB-EC

    GO - Biological processi

    1. phospholipid catabolic process Source: InterPro

    Keywords - Molecular functioni

    Hydrolase

    Keywords - Biological processi

    Lipid degradation, Lipid metabolism

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Lysophospholipase (EC:3.1.1.5)
    Alternative name(s):
    KlPLB
    Phospholipase B
    Gene namesi
    Name:PLB
    Ordered Locus Names:KLLA0C05940g
    OrganismiKluyveromyces lactis (strain ATCC 8585 / CBS 2359 / DSM 70799 / NBRC 1267 / NRRL Y-1140 / WM37) (Yeast) (Candida sphaerica)
    Taxonomic identifieri284590 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeKluyveromyces
    ProteomesiUP000000598: Chromosome C

    Subcellular locationi

    Secreted Curated

    GO - Cellular componenti

    1. extracellular region Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Secreted

    Pathology & Biotechi

    Mutagenesis

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Mutagenesisi112 – 1121R → A: Loss of activity. 1 Publication
    Mutagenesisi406 – 4061D → A: Loss of activity. 1 Publication

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Signal peptidei1 – 2525Sequence AnalysisAdd
    BLAST
    Chaini26 – 640615LysophospholipasePRO_0000024637Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Glycosylationi84 – 841N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi126 – 1261N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi163 – 1631N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi173 – 1731N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi218 – 2181N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi280 – 2801N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi310 – 3101N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi317 – 3171N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi348 – 3481N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi391 – 3911N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi492 – 4921N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi516 – 5161N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi544 – 5441N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi568 – 5681N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi585 – 5851N-linked (GlcNAc...)Sequence Analysis

    Post-translational modificationi

    Highly glycosylated.

    Keywords - PTMi

    Glycoprotein

    Interactioni

    Protein-protein interaction databases

    STRINGi28985.O59863.

    Structurei

    3D structure databases

    ProteinModelPortaliO59863.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini38 – 589552PLA2cPROSITE-ProRule annotationAdd
    BLAST

    Sequence similaritiesi

    Belongs to the lysophospholipase family.Curated
    Contains 1 PLA2c domain.PROSITE-ProRule annotation

    Keywords - Domaini

    Signal

    Phylogenomic databases

    eggNOGiNOG271081.
    HOGENOMiHOG000189547.
    KOiK13333.
    OMAiEDSNSTW.
    OrthoDBiEOG7N37NC.

    Family and domain databases

    InterProiIPR016035. Acyl_Trfase/lysoPLipase.
    IPR002642. LysoPLipase_cat_dom.
    [Graphical view]
    PfamiPF01735. PLA2_B. 1 hit.
    [Graphical view]
    SMARTiSM00022. PLAc. 1 hit.
    [Graphical view]
    SUPFAMiSSF52151. SSF52151. 1 hit.
    PROSITEiPS51210. PLA2C. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    O59863-1 [UniParc]FASTAAdd to Basket

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    MWFLNSVNLL FLVCSVALHL DAVNAWSPTN GYAPGVVDCD ENINLVRKAD    50
    AVSDDEADWL KVRHESTVPA LKDFLQRGFK GFTNDTSIID KLLATQDTAP 100
    KVAIACSGGG YRAMLSGAGM ISAMDNRTDG ANDHGLGGLL QSSTYLAGLS 150
    GGNWLVGTLA YNNWTSVQAI INNMTDDNSI WDISNSIVNP GGINIFSSIS 200
    RWDDISDAVE EKKKAGFNTS ITDVWGRALS YNFFPSLDEG GVGYTWNTLR 250
    DVDVFKNGEM PFPISVAVGR YPGTQVVNLN ATVFEFNPFE MGSWDYTLHT 300
    FTDVRYAGTN VTNGTPNVTG KCVAGFDNTG FVMGTSSSLF NQFLLQLNTT 350
    DLPSFLYNLL HGFLTDASDD YDDISIWAPN PFYEITNIPS NYSQSISEDD 400
    TLYLVDGGED GQNIPLTPLL QTEREIDVIF ALDNSADTDQ SWPDGFSLTQ 450
    TYARQFGLQG KGIAFPYVPD VNTFTNLGLN TRPTFFGCDA RNLTDLESIP 500
    PLVVYMPNTR ESFNSNTSTF KMSYSTSERF KMIQNGFEAV TMKNLTKDEN 550
    FMGCISCAIL RRKQESLNYT LPSECDACFE KYCWNGTVDA TTPISSTTSS 600
    SASSTSTSDS GNKENSARIL APRSTLSLLI GGLASVFISF 640
    Length:640
    Mass (Da):69,983
    Last modified:August 1, 1998 - v1
    Checksum:i90A696CB5D7FF9E3
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AB014495 mRNA. Translation: BAA28619.1.
    CR382123 Genomic DNA. Translation: CAH01313.1.
    RefSeqiXP_452462.1. XM_452462.1.

    Genome annotation databases

    GeneIDi2892361.
    KEGGikla:KLLA0C05940g.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AB014495 mRNA. Translation: BAA28619.1 .
    CR382123 Genomic DNA. Translation: CAH01313.1 .
    RefSeqi XP_452462.1. XM_452462.1.

    3D structure databases

    ProteinModelPortali O59863.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 28985.O59863.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    GeneIDi 2892361.
    KEGGi kla:KLLA0C05940g.

    Phylogenomic databases

    eggNOGi NOG271081.
    HOGENOMi HOG000189547.
    KOi K13333.
    OMAi EDSNSTW.
    OrthoDBi EOG7N37NC.

    Family and domain databases

    InterProi IPR016035. Acyl_Trfase/lysoPLipase.
    IPR002642. LysoPLipase_cat_dom.
    [Graphical view ]
    Pfami PF01735. PLA2_B. 1 hit.
    [Graphical view ]
    SMARTi SM00022. PLAc. 1 hit.
    [Graphical view ]
    SUPFAMi SSF52151. SSF52151. 1 hit.
    PROSITEi PS51210. PLA2C. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Purification and characterization of phospholipase B from Kluyveromyces lactis, and cloning of phospholipase B gene."
      Oishi H., Morimoto T., Watanabe Y., Tamai Y.
      Biosci. Biotechnol. Biochem. 63:83-90(1999) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA], CHARACTERIZATION, MUTAGENESIS OF ARG-112 AND ASP-406.
      Strain: ATCC 56498 / CBS 683 / DSM 4394 / IFO 1090 / NRRL Y-8279.
    2. "Genome evolution in yeasts."
      Dujon B., Sherman D., Fischer G., Durrens P., Casaregola S., Lafontaine I., de Montigny J., Marck C., Neuveglise C., Talla E., Goffard N., Frangeul L., Aigle M., Anthouard V., Babour A., Barbe V., Barnay S., Blanchin S.
      , Beckerich J.-M., Beyne E., Bleykasten C., Boisrame A., Boyer J., Cattolico L., Confanioleri F., de Daruvar A., Despons L., Fabre E., Fairhead C., Ferry-Dumazet H., Groppi A., Hantraye F., Hennequin C., Jauniaux N., Joyet P., Kachouri R., Kerrest A., Koszul R., Lemaire M., Lesur I., Ma L., Muller H., Nicaud J.-M., Nikolski M., Oztas S., Ozier-Kalogeropoulos O., Pellenz S., Potier S., Richard G.-F., Straub M.-L., Suleau A., Swennen D., Tekaia F., Wesolowski-Louvel M., Westhof E., Wirth B., Zeniou-Meyer M., Zivanovic Y., Bolotin-Fukuhara M., Thierry A., Bouchier C., Caudron B., Scarpelli C., Gaillardin C., Weissenbach J., Wincker P., Souciet J.-L.
      Nature 430:35-44(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 8585 / CBS 2359 / DSM 70799 / NBRC 1267 / NRRL Y-1140 / WM37.

    Entry informationi

    Entry nameiPLB_KLULA
    AccessioniPrimary (citable) accession number: O59863
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: October 10, 2003
    Last sequence update: August 1, 1998
    Last modified: October 1, 2014
    This is version 80 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3