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O59863

- PLB_KLULA

UniProt

O59863 - PLB_KLULA

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Protein
Lysophospholipase
Gene
PLB, KLLA0C05940g
Organism
Kluyveromyces lactis (strain ATCC 8585 / CBS 2359 / DSM 70799 / NBRC 1267 / NRRL Y-1140 / WM37) (Yeast) (Candida sphaerica)
Status
Reviewed - Annotation score: 4 out of 5 - Experimental evidence at protein leveli

Functioni

Catalyzes the release of fatty acids from lysophospholipids. At acidic pH the enzyme hydrolyzes all phospholipid substrates without metal ion. On the other hand, at alkaline pH the enzyme shows substrate specificity for phosphatidylcholine and lysophosphatidylcholine and requires Ca2+, Fe3+, or Al3+ for the activity.

Catalytic activityi

2-lysophosphatidylcholine + H2O = glycerophosphocholine + a carboxylate.

pH dependencei

Optimum pH is 2.0 and 7.5.

GO - Molecular functioni

  1. lysophospholipase activity Source: UniProtKB-EC
Complete GO annotation...

GO - Biological processi

  1. phospholipid catabolic process Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Keywords - Biological processi

Lipid degradation, Lipid metabolism

Names & Taxonomyi

Protein namesi
Recommended name:
Lysophospholipase (EC:3.1.1.5)
Alternative name(s):
KlPLB
Phospholipase B
Gene namesi
Name:PLB
Ordered Locus Names:KLLA0C05940g
OrganismiKluyveromyces lactis (strain ATCC 8585 / CBS 2359 / DSM 70799 / NBRC 1267 / NRRL Y-1140 / WM37) (Yeast) (Candida sphaerica)
Taxonomic identifieri284590 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeKluyveromyces
ProteomesiUP000000598: Chromosome C

Subcellular locationi

Secreted Inferred

GO - Cellular componenti

  1. extracellular region Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Secreted

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi112 – 1121R → A: Loss of activity. 1 Publication
Mutagenesisi406 – 4061D → A: Loss of activity. 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2525 Reviewed prediction
Add
BLAST
Chaini26 – 640615Lysophospholipase
PRO_0000024637Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi84 – 841N-linked (GlcNAc...) Reviewed prediction
Glycosylationi126 – 1261N-linked (GlcNAc...) Reviewed prediction
Glycosylationi163 – 1631N-linked (GlcNAc...) Reviewed prediction
Glycosylationi173 – 1731N-linked (GlcNAc...) Reviewed prediction
Glycosylationi218 – 2181N-linked (GlcNAc...) Reviewed prediction
Glycosylationi280 – 2801N-linked (GlcNAc...) Reviewed prediction
Glycosylationi310 – 3101N-linked (GlcNAc...) Reviewed prediction
Glycosylationi317 – 3171N-linked (GlcNAc...) Reviewed prediction
Glycosylationi348 – 3481N-linked (GlcNAc...) Reviewed prediction
Glycosylationi391 – 3911N-linked (GlcNAc...) Reviewed prediction
Glycosylationi492 – 4921N-linked (GlcNAc...) Reviewed prediction
Glycosylationi516 – 5161N-linked (GlcNAc...) Reviewed prediction
Glycosylationi544 – 5441N-linked (GlcNAc...) Reviewed prediction
Glycosylationi568 – 5681N-linked (GlcNAc...) Reviewed prediction
Glycosylationi585 – 5851N-linked (GlcNAc...) Reviewed prediction

Post-translational modificationi

Highly glycosylated.

Keywords - PTMi

Glycoprotein

Interactioni

Protein-protein interaction databases

STRINGi28985.O59863.

Structurei

3D structure databases

ProteinModelPortaliO59863.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini38 – 589552PLA2c
Add
BLAST

Sequence similaritiesi

Belongs to the lysophospholipase family.
Contains 1 PLA2c domain.

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiNOG271081.
HOGENOMiHOG000189547.
KOiK13333.
OMAiEDSNSTW.
OrthoDBiEOG7N37NC.

Family and domain databases

InterProiIPR016035. Acyl_Trfase/lysoPLipase.
IPR002642. LysoPLipase_cat_dom.
[Graphical view]
PfamiPF01735. PLA2_B. 1 hit.
[Graphical view]
SMARTiSM00022. PLAc. 1 hit.
[Graphical view]
SUPFAMiSSF52151. SSF52151. 1 hit.
PROSITEiPS51210. PLA2C. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

O59863-1 [UniParc]FASTAAdd to Basket

« Hide

MWFLNSVNLL FLVCSVALHL DAVNAWSPTN GYAPGVVDCD ENINLVRKAD    50
AVSDDEADWL KVRHESTVPA LKDFLQRGFK GFTNDTSIID KLLATQDTAP 100
KVAIACSGGG YRAMLSGAGM ISAMDNRTDG ANDHGLGGLL QSSTYLAGLS 150
GGNWLVGTLA YNNWTSVQAI INNMTDDNSI WDISNSIVNP GGINIFSSIS 200
RWDDISDAVE EKKKAGFNTS ITDVWGRALS YNFFPSLDEG GVGYTWNTLR 250
DVDVFKNGEM PFPISVAVGR YPGTQVVNLN ATVFEFNPFE MGSWDYTLHT 300
FTDVRYAGTN VTNGTPNVTG KCVAGFDNTG FVMGTSSSLF NQFLLQLNTT 350
DLPSFLYNLL HGFLTDASDD YDDISIWAPN PFYEITNIPS NYSQSISEDD 400
TLYLVDGGED GQNIPLTPLL QTEREIDVIF ALDNSADTDQ SWPDGFSLTQ 450
TYARQFGLQG KGIAFPYVPD VNTFTNLGLN TRPTFFGCDA RNLTDLESIP 500
PLVVYMPNTR ESFNSNTSTF KMSYSTSERF KMIQNGFEAV TMKNLTKDEN 550
FMGCISCAIL RRKQESLNYT LPSECDACFE KYCWNGTVDA TTPISSTTSS 600
SASSTSTSDS GNKENSARIL APRSTLSLLI GGLASVFISF 640
Length:640
Mass (Da):69,983
Last modified:August 1, 1998 - v1
Checksum:i90A696CB5D7FF9E3
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AB014495 mRNA. Translation: BAA28619.1.
CR382123 Genomic DNA. Translation: CAH01313.1.
RefSeqiXP_452462.1. XM_452462.1.

Genome annotation databases

GeneIDi2892361.
KEGGikla:KLLA0C05940g.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AB014495 mRNA. Translation: BAA28619.1 .
CR382123 Genomic DNA. Translation: CAH01313.1 .
RefSeqi XP_452462.1. XM_452462.1.

3D structure databases

ProteinModelPortali O59863.
ModBasei Search...

Protein-protein interaction databases

STRINGi 28985.O59863.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

GeneIDi 2892361.
KEGGi kla:KLLA0C05940g.

Phylogenomic databases

eggNOGi NOG271081.
HOGENOMi HOG000189547.
KOi K13333.
OMAi EDSNSTW.
OrthoDBi EOG7N37NC.

Family and domain databases

InterProi IPR016035. Acyl_Trfase/lysoPLipase.
IPR002642. LysoPLipase_cat_dom.
[Graphical view ]
Pfami PF01735. PLA2_B. 1 hit.
[Graphical view ]
SMARTi SM00022. PLAc. 1 hit.
[Graphical view ]
SUPFAMi SSF52151. SSF52151. 1 hit.
PROSITEi PS51210. PLA2C. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Purification and characterization of phospholipase B from Kluyveromyces lactis, and cloning of phospholipase B gene."
    Oishi H., Morimoto T., Watanabe Y., Tamai Y.
    Biosci. Biotechnol. Biochem. 63:83-90(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], CHARACTERIZATION, MUTAGENESIS OF ARG-112 AND ASP-406.
    Strain: ATCC 56498 / CBS 683 / DSM 4394 / IFO 1090 / NRRL Y-8279.
  2. "Genome evolution in yeasts."
    Dujon B., Sherman D., Fischer G., Durrens P., Casaregola S., Lafontaine I., de Montigny J., Marck C., Neuveglise C., Talla E., Goffard N., Frangeul L., Aigle M., Anthouard V., Babour A., Barbe V., Barnay S., Blanchin S.
    , Beckerich J.-M., Beyne E., Bleykasten C., Boisrame A., Boyer J., Cattolico L., Confanioleri F., de Daruvar A., Despons L., Fabre E., Fairhead C., Ferry-Dumazet H., Groppi A., Hantraye F., Hennequin C., Jauniaux N., Joyet P., Kachouri R., Kerrest A., Koszul R., Lemaire M., Lesur I., Ma L., Muller H., Nicaud J.-M., Nikolski M., Oztas S., Ozier-Kalogeropoulos O., Pellenz S., Potier S., Richard G.-F., Straub M.-L., Suleau A., Swennen D., Tekaia F., Wesolowski-Louvel M., Westhof E., Wirth B., Zeniou-Meyer M., Zivanovic Y., Bolotin-Fukuhara M., Thierry A., Bouchier C., Caudron B., Scarpelli C., Gaillardin C., Weissenbach J., Wincker P., Souciet J.-L.
    Nature 430:35-44(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 8585 / CBS 2359 / DSM 70799 / NBRC 1267 / NRRL Y-1140 / WM37.

Entry informationi

Entry nameiPLB_KLULA
AccessioniPrimary (citable) accession number: O59863
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 10, 2003
Last sequence update: August 1, 1998
Last modified: November 13, 2013
This is version 79 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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