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Protein

Lysophospholipase

Gene

PLB

Organism
Kluyveromyces lactis (strain ATCC 8585 / CBS 2359 / DSM 70799 / NBRC 1267 / NRRL Y-1140 / WM37) (Yeast) (Candida sphaerica)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Catalyzes the release of fatty acids from lysophospholipids. At acidic pH the enzyme hydrolyzes all phospholipid substrates without metal ion. On the other hand, at alkaline pH the enzyme shows substrate specificity for phosphatidylcholine and lysophosphatidylcholine and requires Ca2+, Fe3+, or Al3+ for the activity.

Catalytic activityi

2-lysophosphatidylcholine + H2O = glycerophosphocholine + a carboxylate.

pH dependencei

Optimum pH is 2.0 and 7.5.

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Keywords - Biological processi

Lipid degradation, Lipid metabolism

Enzyme and pathway databases

BRENDAi3.1.1.5. 2825.

Names & Taxonomyi

Protein namesi
Recommended name:
Lysophospholipase (EC:3.1.1.5)
Alternative name(s):
KlPLB
Phospholipase B
Gene namesi
Name:PLB
Ordered Locus Names:KLLA0C05940g
OrganismiKluyveromyces lactis (strain ATCC 8585 / CBS 2359 / DSM 70799 / NBRC 1267 / NRRL Y-1140 / WM37) (Yeast) (Candida sphaerica)
Taxonomic identifieri284590 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeKluyveromyces
Proteomesi
  • UP000000598 Componenti: Chromosome C

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Secreted

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi112R → A: Loss of activity. 1 Publication1
Mutagenesisi406D → A: Loss of activity. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 25Sequence analysisAdd BLAST25
ChainiPRO_000002463726 – 640LysophospholipaseAdd BLAST615

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi84N-linked (GlcNAc...)Sequence analysis1
Glycosylationi126N-linked (GlcNAc...)Sequence analysis1
Glycosylationi163N-linked (GlcNAc...)Sequence analysis1
Glycosylationi173N-linked (GlcNAc...)Sequence analysis1
Glycosylationi218N-linked (GlcNAc...)Sequence analysis1
Glycosylationi280N-linked (GlcNAc...)Sequence analysis1
Glycosylationi310N-linked (GlcNAc...)Sequence analysis1
Glycosylationi317N-linked (GlcNAc...)Sequence analysis1
Glycosylationi348N-linked (GlcNAc...)Sequence analysis1
Glycosylationi391N-linked (GlcNAc...)Sequence analysis1
Glycosylationi492N-linked (GlcNAc...)Sequence analysis1
Glycosylationi516N-linked (GlcNAc...)Sequence analysis1
Glycosylationi544N-linked (GlcNAc...)Sequence analysis1
Glycosylationi568N-linked (GlcNAc...)Sequence analysis1
Glycosylationi585N-linked (GlcNAc...)Sequence analysis1

Post-translational modificationi

Highly glycosylated.

Keywords - PTMi

Glycoprotein

Interactioni

Protein-protein interaction databases

STRINGi284590.XP_452462.1.

Structurei

3D structure databases

ProteinModelPortaliO59863.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini38 – 589PLA2cPROSITE-ProRule annotationAdd BLAST552

Sequence similaritiesi

Belongs to the lysophospholipase family.Curated
Contains 1 PLA2c domain.PROSITE-ProRule annotation

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiKOG1325. Eukaryota.
ENOG410XR72. LUCA.
HOGENOMiHOG000189547.
InParanoidiO59863.
KOiK13333.
OMAiEDSNSTW.
OrthoDBiEOG092C2IZ9.

Family and domain databases

InterProiIPR016035. Acyl_Trfase/lysoPLipase.
IPR002642. LysoPLipase_cat_dom.
[Graphical view]
PfamiPF01735. PLA2_B. 1 hit.
[Graphical view]
SMARTiSM00022. PLAc. 1 hit.
[Graphical view]
SUPFAMiSSF52151. SSF52151. 1 hit.
PROSITEiPS51210. PLA2C. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

O59863-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MWFLNSVNLL FLVCSVALHL DAVNAWSPTN GYAPGVVDCD ENINLVRKAD
60 70 80 90 100
AVSDDEADWL KVRHESTVPA LKDFLQRGFK GFTNDTSIID KLLATQDTAP
110 120 130 140 150
KVAIACSGGG YRAMLSGAGM ISAMDNRTDG ANDHGLGGLL QSSTYLAGLS
160 170 180 190 200
GGNWLVGTLA YNNWTSVQAI INNMTDDNSI WDISNSIVNP GGINIFSSIS
210 220 230 240 250
RWDDISDAVE EKKKAGFNTS ITDVWGRALS YNFFPSLDEG GVGYTWNTLR
260 270 280 290 300
DVDVFKNGEM PFPISVAVGR YPGTQVVNLN ATVFEFNPFE MGSWDYTLHT
310 320 330 340 350
FTDVRYAGTN VTNGTPNVTG KCVAGFDNTG FVMGTSSSLF NQFLLQLNTT
360 370 380 390 400
DLPSFLYNLL HGFLTDASDD YDDISIWAPN PFYEITNIPS NYSQSISEDD
410 420 430 440 450
TLYLVDGGED GQNIPLTPLL QTEREIDVIF ALDNSADTDQ SWPDGFSLTQ
460 470 480 490 500
TYARQFGLQG KGIAFPYVPD VNTFTNLGLN TRPTFFGCDA RNLTDLESIP
510 520 530 540 550
PLVVYMPNTR ESFNSNTSTF KMSYSTSERF KMIQNGFEAV TMKNLTKDEN
560 570 580 590 600
FMGCISCAIL RRKQESLNYT LPSECDACFE KYCWNGTVDA TTPISSTTSS
610 620 630 640
SASSTSTSDS GNKENSARIL APRSTLSLLI GGLASVFISF
Length:640
Mass (Da):69,983
Last modified:August 1, 1998 - v1
Checksum:i90A696CB5D7FF9E3
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB014495 mRNA. Translation: BAA28619.1.
CR382123 Genomic DNA. Translation: CAH01313.1.
RefSeqiXP_452462.1. XM_452462.1.

Genome annotation databases

EnsemblFungiiCAH01313; CAH01313; KLLA0_C05940g.
GeneIDi2892361.
KEGGikla:KLLA0C05940g.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB014495 mRNA. Translation: BAA28619.1.
CR382123 Genomic DNA. Translation: CAH01313.1.
RefSeqiXP_452462.1. XM_452462.1.

3D structure databases

ProteinModelPortaliO59863.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi284590.XP_452462.1.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiCAH01313; CAH01313; KLLA0_C05940g.
GeneIDi2892361.
KEGGikla:KLLA0C05940g.

Phylogenomic databases

eggNOGiKOG1325. Eukaryota.
ENOG410XR72. LUCA.
HOGENOMiHOG000189547.
InParanoidiO59863.
KOiK13333.
OMAiEDSNSTW.
OrthoDBiEOG092C2IZ9.

Enzyme and pathway databases

BRENDAi3.1.1.5. 2825.

Family and domain databases

InterProiIPR016035. Acyl_Trfase/lysoPLipase.
IPR002642. LysoPLipase_cat_dom.
[Graphical view]
PfamiPF01735. PLA2_B. 1 hit.
[Graphical view]
SMARTiSM00022. PLAc. 1 hit.
[Graphical view]
SUPFAMiSSF52151. SSF52151. 1 hit.
PROSITEiPS51210. PLA2C. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiPLB_KLULA
AccessioniPrimary (citable) accession number: O59863
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 10, 2003
Last sequence update: August 1, 1998
Last modified: September 7, 2016
This is version 87 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.