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Protein

Lysophospholipase

Gene

PLB

Organism
Kluyveromyces lactis (strain ATCC 8585 / CBS 2359 / DSM 70799 / NBRC 1267 / NRRL Y-1140 / WM37) (Yeast) (Candida sphaerica)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Catalyzes the release of fatty acids from lysophospholipids. At acidic pH the enzyme hydrolyzes all phospholipid substrates without metal ion. On the other hand, at alkaline pH the enzyme shows substrate specificity for phosphatidylcholine and lysophosphatidylcholine and requires Ca2+, Fe3+, or Al3+ for the activity.

Catalytic activityi

2-lysophosphatidylcholine + H2O = glycerophosphocholine + a carboxylate.

pH dependencei

Optimum pH is 2.0 and 7.5.

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Keywords - Biological processi

Lipid degradation, Lipid metabolism

Enzyme and pathway databases

BRENDAi3.1.1.5. 2825.

Names & Taxonomyi

Protein namesi
Recommended name:
Lysophospholipase (EC:3.1.1.5)
Alternative name(s):
KlPLB
Phospholipase B
Gene namesi
Name:PLB
Ordered Locus Names:KLLA0C05940g
OrganismiKluyveromyces lactis (strain ATCC 8585 / CBS 2359 / DSM 70799 / NBRC 1267 / NRRL Y-1140 / WM37) (Yeast) (Candida sphaerica)
Taxonomic identifieri284590 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeKluyveromyces
ProteomesiUP000000598 Componenti: Chromosome C

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Secreted

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi112 – 1121R → A: Loss of activity. 1 Publication
Mutagenesisi406 – 4061D → A: Loss of activity. 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2525Sequence AnalysisAdd
BLAST
Chaini26 – 640615LysophospholipasePRO_0000024637Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi84 – 841N-linked (GlcNAc...)Sequence Analysis
Glycosylationi126 – 1261N-linked (GlcNAc...)Sequence Analysis
Glycosylationi163 – 1631N-linked (GlcNAc...)Sequence Analysis
Glycosylationi173 – 1731N-linked (GlcNAc...)Sequence Analysis
Glycosylationi218 – 2181N-linked (GlcNAc...)Sequence Analysis
Glycosylationi280 – 2801N-linked (GlcNAc...)Sequence Analysis
Glycosylationi310 – 3101N-linked (GlcNAc...)Sequence Analysis
Glycosylationi317 – 3171N-linked (GlcNAc...)Sequence Analysis
Glycosylationi348 – 3481N-linked (GlcNAc...)Sequence Analysis
Glycosylationi391 – 3911N-linked (GlcNAc...)Sequence Analysis
Glycosylationi492 – 4921N-linked (GlcNAc...)Sequence Analysis
Glycosylationi516 – 5161N-linked (GlcNAc...)Sequence Analysis
Glycosylationi544 – 5441N-linked (GlcNAc...)Sequence Analysis
Glycosylationi568 – 5681N-linked (GlcNAc...)Sequence Analysis
Glycosylationi585 – 5851N-linked (GlcNAc...)Sequence Analysis

Post-translational modificationi

Highly glycosylated.

Keywords - PTMi

Glycoprotein

Interactioni

Protein-protein interaction databases

STRINGi28985.O59863.

Structurei

3D structure databases

ProteinModelPortaliO59863.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini38 – 589552PLA2cPROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Belongs to the lysophospholipase family.Curated
Contains 1 PLA2c domain.PROSITE-ProRule annotation

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiNOG271081.
HOGENOMiHOG000189547.
InParanoidiO59863.
KOiK13333.
OMAiYQFINAS.
OrthoDBiEOG7N37NC.

Family and domain databases

InterProiIPR016035. Acyl_Trfase/lysoPLipase.
IPR002642. LysoPLipase_cat_dom.
[Graphical view]
PfamiPF01735. PLA2_B. 1 hit.
[Graphical view]
SMARTiSM00022. PLAc. 1 hit.
[Graphical view]
SUPFAMiSSF52151. SSF52151. 1 hit.
PROSITEiPS51210. PLA2C. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

O59863-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MWFLNSVNLL FLVCSVALHL DAVNAWSPTN GYAPGVVDCD ENINLVRKAD
60 70 80 90 100
AVSDDEADWL KVRHESTVPA LKDFLQRGFK GFTNDTSIID KLLATQDTAP
110 120 130 140 150
KVAIACSGGG YRAMLSGAGM ISAMDNRTDG ANDHGLGGLL QSSTYLAGLS
160 170 180 190 200
GGNWLVGTLA YNNWTSVQAI INNMTDDNSI WDISNSIVNP GGINIFSSIS
210 220 230 240 250
RWDDISDAVE EKKKAGFNTS ITDVWGRALS YNFFPSLDEG GVGYTWNTLR
260 270 280 290 300
DVDVFKNGEM PFPISVAVGR YPGTQVVNLN ATVFEFNPFE MGSWDYTLHT
310 320 330 340 350
FTDVRYAGTN VTNGTPNVTG KCVAGFDNTG FVMGTSSSLF NQFLLQLNTT
360 370 380 390 400
DLPSFLYNLL HGFLTDASDD YDDISIWAPN PFYEITNIPS NYSQSISEDD
410 420 430 440 450
TLYLVDGGED GQNIPLTPLL QTEREIDVIF ALDNSADTDQ SWPDGFSLTQ
460 470 480 490 500
TYARQFGLQG KGIAFPYVPD VNTFTNLGLN TRPTFFGCDA RNLTDLESIP
510 520 530 540 550
PLVVYMPNTR ESFNSNTSTF KMSYSTSERF KMIQNGFEAV TMKNLTKDEN
560 570 580 590 600
FMGCISCAIL RRKQESLNYT LPSECDACFE KYCWNGTVDA TTPISSTTSS
610 620 630 640
SASSTSTSDS GNKENSARIL APRSTLSLLI GGLASVFISF
Length:640
Mass (Da):69,983
Last modified:August 1, 1998 - v1
Checksum:i90A696CB5D7FF9E3
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB014495 mRNA. Translation: BAA28619.1.
CR382123 Genomic DNA. Translation: CAH01313.1.
RefSeqiXP_452462.1. XM_452462.1.

Genome annotation databases

GeneIDi2892361.
KEGGikla:KLLA0C05940g.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB014495 mRNA. Translation: BAA28619.1.
CR382123 Genomic DNA. Translation: CAH01313.1.
RefSeqiXP_452462.1. XM_452462.1.

3D structure databases

ProteinModelPortaliO59863.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi28985.O59863.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi2892361.
KEGGikla:KLLA0C05940g.

Phylogenomic databases

eggNOGiNOG271081.
HOGENOMiHOG000189547.
InParanoidiO59863.
KOiK13333.
OMAiYQFINAS.
OrthoDBiEOG7N37NC.

Enzyme and pathway databases

BRENDAi3.1.1.5. 2825.

Family and domain databases

InterProiIPR016035. Acyl_Trfase/lysoPLipase.
IPR002642. LysoPLipase_cat_dom.
[Graphical view]
PfamiPF01735. PLA2_B. 1 hit.
[Graphical view]
SMARTiSM00022. PLAc. 1 hit.
[Graphical view]
SUPFAMiSSF52151. SSF52151. 1 hit.
PROSITEiPS51210. PLA2C. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Purification and characterization of phospholipase B from Kluyveromyces lactis, and cloning of phospholipase B gene."
    Oishi H., Morimoto T., Watanabe Y., Tamai Y.
    Biosci. Biotechnol. Biochem. 63:83-90(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], CHARACTERIZATION, MUTAGENESIS OF ARG-112 AND ASP-406.
    Strain: ATCC 56498 / CBS 683 / DSM 4394 / IFO 1090 / NRRL Y-8279.
  2. "Genome evolution in yeasts."
    Dujon B., Sherman D., Fischer G., Durrens P., Casaregola S., Lafontaine I., de Montigny J., Marck C., Neuveglise C., Talla E., Goffard N., Frangeul L., Aigle M., Anthouard V., Babour A., Barbe V., Barnay S., Blanchin S.
    , Beckerich J.-M., Beyne E., Bleykasten C., Boisrame A., Boyer J., Cattolico L., Confanioleri F., de Daruvar A., Despons L., Fabre E., Fairhead C., Ferry-Dumazet H., Groppi A., Hantraye F., Hennequin C., Jauniaux N., Joyet P., Kachouri R., Kerrest A., Koszul R., Lemaire M., Lesur I., Ma L., Muller H., Nicaud J.-M., Nikolski M., Oztas S., Ozier-Kalogeropoulos O., Pellenz S., Potier S., Richard G.-F., Straub M.-L., Suleau A., Swennen D., Tekaia F., Wesolowski-Louvel M., Westhof E., Wirth B., Zeniou-Meyer M., Zivanovic Y., Bolotin-Fukuhara M., Thierry A., Bouchier C., Caudron B., Scarpelli C., Gaillardin C., Weissenbach J., Wincker P., Souciet J.-L.
    Nature 430:35-44(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 8585 / CBS 2359 / DSM 70799 / NBRC 1267 / NRRL Y-1140 / WM37.

Entry informationi

Entry nameiPLB_KLULA
AccessioniPrimary (citable) accession number: O59863
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 10, 2003
Last sequence update: August 1, 1998
Last modified: April 1, 2015
This is version 83 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.