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Protein

Glutathione peroxidase-like peroxiredoxin gpx1

Gene

gpx1

Organism
Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Glutathione peroxidase-like protein that protects cells during oxidative stress. Has peroxidase activity reducing hydrogen peroxide, alkyl and phospholipid hydroperoxides using preferentially thioredoxin as a reducing power. May act as a scavenger of H2O2.2 Publications

Miscellaneous

The active site is a conserved redox-active cysteine residue, the peroxidatic cysteine (C(P)), which makes the nucleophilic attack on the peroxide substrate. The peroxide oxidizes the C(P)-SH to cysteine sulfenic acid (C(P)-SOH), which then reacts with another cysteine residue, the resolving cysteine (C(R)), to form a disulfide bridge. The disulfide is subsequently reduced by an appropriate electron donor to complete the catalytic cycle. In this atypical 2-Cys peroxiredoxin, C(R) is present in the same subunit to form an intramolecular disulfide.By similarity

Catalytic activityi

2 R'-SH + ROOH = R'-S-S-R' + H2O + ROH.1 Publication

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei36Cysteine sulfenic acid (-SOH) intermediateBy similarity1

GO - Molecular functioni

  • thioredoxin peroxidase activity Source: PomBase

GO - Biological processi

  • cell redox homeostasis Source: PomBase
  • cellular detoxification of hydrogen peroxide Source: PomBase
  • hydrogen peroxide catabolic process Source: PomBase

Keywordsi

Molecular functionAntioxidant, Oxidoreductase, Peroxidase
Biological processStress response

Enzyme and pathway databases

ReactomeiR-SPO-2142712. Synthesis of 12-eicosatetraenoic acid derivatives.
R-SPO-2142770. Synthesis of 15-eicosatetraenoic acid derivatives.
R-SPO-3299685. Detoxification of Reactive Oxygen Species.
R-SPO-5628897. TP53 Regulates Metabolic Genes.
R-SPO-74259. Purine catabolism.

Protein family/group databases

PeroxiBasei3744. SpomGPx01.

Names & Taxonomyi

Protein namesi
Recommended name:
Glutathione peroxidase-like peroxiredoxin gpx1Curated (EC:1.11.1.151 Publication)
Alternative name(s):
Glutathione peroxidase homolog1 Publication
Short name:
GPx
Thioredoxin peroxidase gpx11 Publication
Gene namesi
Name:gpx11 Publication
ORF Names:SPBC32F12.03cImported
OrganismiSchizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Taxonomic identifieri284812 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaTaphrinomycotinaSchizosaccharomycetesSchizosaccharomycetalesSchizosaccharomycetaceaeSchizosaccharomyces
Proteomesi
  • UP000002485 Componenti: Chromosome II

Organism-specific databases

EuPathDBiFungiDB:SPBC32F12.03c.
PomBaseiSPBC32F12.03c. gpx1.

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm, Mitochondrion

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000666441 – 158Glutathione peroxidase-like peroxiredoxin gpx1Add BLAST158

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Disulfide bondi36 ↔ 82Redox-activeBy similarity

Keywords - PTMi

Disulfide bond

Proteomic databases

MaxQBiO59858.
PRIDEiO59858.

PTM databases

iPTMnetiO59858.

Expressioni

Inductioni

By oxidative, osmo- and heat stress.1 Publication

Interactioni

Subunit structurei

Monomer.1 Publication

Protein-protein interaction databases

BioGridi276755. 12 interactors.
MINTiMINT-4676657.
STRINGi4896.SPBC32F12.03c.1.

Structurei

3D structure databases

ProteinModelPortaliO59858.
SMRiO59858.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the glutathione peroxidase family.Curated

Keywords - Domaini

Redox-active center

Phylogenomic databases

HOGENOMiHOG000277054.
InParanoidiO59858.
KOiK00432.
OMAiIAQTCHI.
OrthoDBiEOG092C4H0P.
PhylomeDBiO59858.

Family and domain databases

CDDicd00340. GSH_Peroxidase. 1 hit.
InterProiView protein in InterPro
IPR000889. Glutathione_peroxidase.
IPR029759. GPX_AS.
IPR029760. GPX_CS.
IPR036249. Thioredoxin-like_sf.
PANTHERiPTHR11592. PTHR11592. 1 hit.
PfamiView protein in Pfam
PF00255. GSHPx. 1 hit.
PIRSFiPIRSF000303. Glutathion_perox. 1 hit.
PRINTSiPR01011. GLUTPROXDASE.
SUPFAMiSSF52833. SSF52833. 1 hit.
PROSITEiView protein in PROSITE
PS00460. GLUTATHIONE_PEROXID_1. 1 hit.
PS00763. GLUTATHIONE_PEROXID_2. 1 hit.
PS51355. GLUTATHIONE_PEROXID_3. 1 hit.

Sequencei

Sequence statusi: Complete.

O59858-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSHFYDLAPK DKDGNPFPFS NLKGKVVLVV NTASKCGFTP QYKGLEALYQ
60 70 80 90 100
KYKDRGFIIL GFPCNQFGNQ EPGSDEEIAQ FCQKNYGVTF PVLAKINVNG
110 120 130 140 150
DNVDPVYQFL KSQKKQLGLE RIKWNFEKFL VNRQGQVIER YSSISKPEHL

ENDIESVL
Length:158
Mass (Da):18,061
Last modified:August 1, 1998 - v1
Checksum:i2D7009280B676876
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB012395 Genomic DNA. Translation: BAA25326.1.
CU329671 Genomic DNA. Translation: CAA19364.1.
PIRiT43376.
RefSeqiNP_596146.1. NM_001022065.2.

Genome annotation databases

EnsemblFungiiSPBC32F12.03c.1; SPBC32F12.03c.1:pep; SPBC32F12.03c.
GeneIDi2540222.
KEGGispo:SPBC32F12.03c.

Similar proteinsi

Entry informationi

Entry nameiGPX1_SCHPO
AccessioniPrimary (citable) accession number: O59858
Entry historyiIntegrated into UniProtKB/Swiss-Prot: May 30, 2000
Last sequence update: August 1, 1998
Last modified: October 25, 2017
This is version 127 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Caution

Was originally thought to be a glutathione peroxidase (PubMed:10455235), but functions as an atypical 2-Cys peroxiredoxin using thioredoxin as reducing power instead (PubMed:18162174).2 Publications

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Schizosaccharomyces pombe
    Schizosaccharomyces pombe: entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families