Reviewed, UniProtKB/Swiss-Prot O59853 (HOG2_ZYGRO)

Names and origin

Protein names

Recommended name:
    Mitogen-activated protein kinase HOG2
      Short name=MAP kinase HOG2
    EC=2.7.11.24
Alternative name(s):
    ZrHOG2

Gene names

Name:

HOG2

Organism

Zygosaccharomyces rouxii (Candida mogii)

Taxonomic identifier

4956 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Zygosaccharomyces

Protein attributes

Sequence length	420 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Mitogen-activated protein kinase involved in a signal transduction pathway that is activated by changes in the osmolarity of the extracellular environment. Controls osmotic regulation of transcription of target genes. Ref.1
Catalytic activity	ATP + a protein = ADP + a phosphoprotein.
Cofactor	Magnesium By similarity.
Enzyme regulation	Activated by tyrosine and threonine phosphorylation By similarity.
Subcellular location	Cytoplasm By similarity. Nucleus By similarity.
Domain	The TXY motif contains the threonine and tyrosine residues whose phosphorylation activates the MAP kinases.
Post-translational modification	Dually phosphorylated on Thr-174 and Tyr-176, which activates the enzyme By similarity.
Sequence similarities	Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. MAP kinase subfamily. HOG1 sub-subfamily. Contains 1 protein kinase domain.

Ontologies

Keywords
Biological process	Transcription Transcription regulation
Cellular component	Cytoplasm Nucleus
Ligand	ATP-binding Nucleotide-binding
Molecular function	Activator Kinase Serine/threonine-protein kinase Transferase
PTM	Phosphoprotein
Gene Ontology (GO)
Biological process	protein amino acid phosphorylation Inferred from electronic annotation. Source: InterPro regulation of transcription Inferred from electronic annotation. Source: UniProtKB-KW transcription Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell nucleus Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW MAP kinase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 420	420	Mitogen-activated protein kinase HOG2		PRO_0000289711
Regions
	Nucleotide binding	29 – 37	9	ATP By similarity
	Motif	174 – 176	3	TXY
Sites
	Active site	144	1	Proton acceptor By similarity
	Binding site	52	1	ATP By similarity
Amino acid modifications
	Modified residue	174	1	Phosphothreonine By similarity
	Modified residue	176	1	Phosphotyrosine By similarity

Sequences

	Sequence		Length	Mass (Da)
	O59853-1 [UniParc]. Last modified August 1, 1998. Version 1. Checksum: C24E97A8EBD3EBAA	FASTA	420	47,593
	10 20 30 40 50 60 MATHEEFIRT QVFGTVFEIT NRYTDLNPVG MGAFGLVCSA TDTLVGQPVA IKKIMKPFST 70 80 90 100 110 120 AVLAKRTYRE LKLLKHLRHE NLICLQDIFL SPLEDIYFVT ELQGTDLHRL LQTRPLEKQF 130 140 150 160 170 180 VQYFLYQILR GLKYVHSAGV IHRDLKPSNI LINENCDLKI CDFGLARIQD PQMTGYVSTR 190 200 210 220 230 240 YYRAPEIMLT WQKYDVEVDI WSAGCIFSEM IEGKPLFPGK DHVHQFSIIT DLLGSPPRDV 250 260 270 280 290 300 INTICSENTL KFVTSLPHRD PVPFQERFKT VEPDAVDLLR RMLVFVPKKR ITAADALVHP 310 320 330 340 350 360 YLAPYHDPTD EPTAEAQFDW DFNDADLPVD TWRVMMYSEI LDFHKIGGGD GQIDTNAAFD 370 380 390 400 410 420 DQVAAAHAAA MAQHHHQTQQ QSSGKHTNPT TSSSAATITV TVPIAITMPS LIMVIKRFTT

References

[1]

"Two putative MAP kinase genes, ZrHOG1 and ZrHOG2, cloned from the salt-tolerant yeast Zygosaccharomyces rouxii are functionally homologous to the Saccharomyces cerevisiae HOG1 gene." Iwaki T., Tamai Y., Watanabe Y. Microbiology 145:241-248(1999) [PubMed: 10206704] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION. Strain: ATCC 42981 / IAM 12879 / JCM 22060 / S-96.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AB012088 Genomic DNA. Translation: BAA25143.1.
3D structure databases
HSSP	HSSP built from PDB template 1KV1 based on UniProtKB Q16539.
ModBase	Search...
Enzyme and pathway databases
BRENDA	2.7.11.24. 266875.
Family and domain databases
InterPro	IPR003527. MAP_kinase_CS. IPR000719. Prot_kinase_core. IPR017441. Protein_kinase_ATP_BS. IPR017442. Se/Thr_pkinase-rel. IPR008271. Ser_thr_pkin_AS. IPR002290. Ser_thr_pkinase. [Graphical view]
Pfam	PF00069. Pkinase. 1 hit. [Graphical view]
ProDom	PD000001. Prot_kinase. 1 hit. [Graphical view] [Entries sharing at least one domain]
SMART	SM00220. S_TKc. 1 hit. [Graphical view]
PROSITE	PS01351. MAPK. 1 hit. PS00107. PROTEIN_KINASE_ATP. 1 hit. PS50011. PROTEIN_KINASE_DOM. 1 hit. PS00108. PROTEIN_KINASE_ST. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

HOG2_ZYGRO

Accession

Primary (citable) accession number: O59853

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 29, 2007
Last sequence update:	August 1, 1998
Last modified:	September 22, 2009
This is version 55 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

FPAP (Fungal Proteome Annotation Project)