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Protein

Glyceraldehyde-3-phosphate dehydrogenase

Gene

GPD

Organism
Ogataea parapolymorpha (strain ATCC 26012 / BCRC 20466 / JCM 22074 / NRRL Y-7560 / DL-1) (Yeast) (Hansenula polymorpha)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalytic activityi

D-glyceraldehyde 3-phosphate + phosphate + NAD+ = 3-phospho-D-glyceroyl phosphate + NADH.PROSITE-ProRule annotation

Pathwayi: glycolysis

This protein is involved in step 1 of the subpathway that synthesizes pyruvate from D-glyceraldehyde 3-phosphate.
Proteins known to be involved in the 5 steps of the subpathway in this organism are:
  1. Glyceraldehyde-3-phosphate dehydrogenase (GPD)
  2. no protein annotated in this organism
  3. Phosphoglycerate mutase (HPODL_00350), Phosphoglycerate mutase (HPODL_01332)
  4. no protein annotated in this organism
  5. Pyruvate kinase (HPODL_01513)
This subpathway is part of the pathway glycolysis, which is itself part of Carbohydrate degradation.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes pyruvate from D-glyceraldehyde 3-phosphate, the pathway glycolysis and in Carbohydrate degradation.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei34 – 341NADBy similarity
Binding sitei79 – 791NAD; via carbonyl oxygenBy similarity
Active sitei151 – 1511NucleophilePROSITE-ProRule annotation
Sitei178 – 1781Activates thiol group during catalysisBy similarity
Binding sitei181 – 1811Glyceraldehyde 3-phosphateBy similarity
Binding sitei233 – 2331Glyceraldehyde 3-phosphateBy similarity
Binding sitei315 – 3151NADBy similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi12 – 132NADBy similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

Glycolysis

Keywords - Ligandi

NAD

Enzyme and pathway databases

BioCyciMetaCyc:MONOMER-13168.
UniPathwayiUPA00109; UER00184.

Names & Taxonomyi

Protein namesi
Recommended name:
Glyceraldehyde-3-phosphate dehydrogenase (EC:1.2.1.12)
Short name:
GAPDH
Gene namesi
Name:GPD
ORF Names:HPODL_04957
OrganismiOgataea parapolymorpha (strain ATCC 26012 / BCRC 20466 / JCM 22074 / NRRL Y-7560 / DL-1) (Yeast) (Hansenula polymorpha)
Taxonomic identifieri871575 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesPichiaceaeOgataea
Proteomesi
  • UP000008673 Componentsi: Chromosome II, Partially assembled WGS sequence

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 335335Glyceraldehyde-3-phosphate dehydrogenasePRO_0000145570Add
BLAST

Interactioni

Subunit structurei

Homotetramer.By similarity

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni150 – 1523Glyceraldehyde 3-phosphate bindingBy similarity
Regioni210 – 2112Glyceraldehyde 3-phosphate bindingBy similarity

Sequence similaritiesi

Phylogenomic databases

OrthoDBiEOG092C2MZH.

Family and domain databases

Gene3Di3.40.50.720. 1 hit.
InterProiIPR020831. GlycerAld/Erythrose_P_DH.
IPR020830. GlycerAld_3-P_DH_AS.
IPR020829. GlycerAld_3-P_DH_cat.
IPR020828. GlycerAld_3-P_DH_NAD(P)-bd.
IPR006424. Glyceraldehyde-3-P_DH_1.
IPR016040. NAD(P)-bd_dom.
[Graphical view]
PANTHERiPTHR10836. PTHR10836. 1 hit.
PfamiPF02800. Gp_dh_C. 1 hit.
PF00044. Gp_dh_N. 1 hit.
[Graphical view]
PIRSFiPIRSF000149. GAP_DH. 1 hit.
PRINTSiPR00078. G3PDHDRGNASE.
SMARTiSM00846. Gp_dh_N. 1 hit.
[Graphical view]
SUPFAMiSSF51735. SSF51735. 1 hit.
TIGRFAMsiTIGR01534. GAPDH-I. 1 hit.
PROSITEiPS00071. GAPDH. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

O59841-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTANVGINGF GRIGRLVLRI ALSRDDINVI AINDPFIAPD YAAYMFKYDS
60 70 80 90 100
THGKFKGTVT HEGKYLVING KKIEVFQERD PANIPWGKEG VDYVLDSTGV
110 120 130 140 150
FTTIEGAQKH IDAGAKKVII TAPSKDAPMF VVGVNHEEYT PDIKILSNAS
160 170 180 190 200
CTTNCLAPLA KVINDTYGIE EGLMTTIHSI TATQKTVDGP SHKDWRGGRT
210 220 230 240 250
ASGNIIPSST GAAKAVGKVL PALAGKLTGM SMRVPTTDVS VVDLTVNLKK
260 270 280 290 300
PTTYEDICAT MKKAAEGPLA GILGYTDEAV VSSDFLTDSR SSVFDAKAGI
310 320 330
LLTPTFVKLV SWYDNEYGYS TRVVDLLQHV AKVSA
Length:335
Mass (Da):35,951
Last modified:May 31, 2011 - v2
Checksum:i3C744B9B4839C313
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti166 – 1672TY → IF in AAC08320 (Ref. 1) Curated
Sequence conflicti177 – 1771I → V in AAC08320 (Ref. 1) Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U95625 Genomic DNA. Translation: AAC08320.1.
AEOI02000004 Genomic DNA. Translation: ESX02203.1.
PIRiT12046.
RefSeqiXP_013936789.1. XM_014081314.1.

Genome annotation databases

EnsemblFungiiESX02203; ESX02203; HPODL_04957.
GeneIDi25774380.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U95625 Genomic DNA. Translation: AAC08320.1.
AEOI02000004 Genomic DNA. Translation: ESX02203.1.
PIRiT12046.
RefSeqiXP_013936789.1. XM_014081314.1.

3D structure databases

ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiESX02203; ESX02203; HPODL_04957.
GeneIDi25774380.

Phylogenomic databases

OrthoDBiEOG092C2MZH.

Enzyme and pathway databases

UniPathwayiUPA00109; UER00184.
BioCyciMetaCyc:MONOMER-13168.

Family and domain databases

Gene3Di3.40.50.720. 1 hit.
InterProiIPR020831. GlycerAld/Erythrose_P_DH.
IPR020830. GlycerAld_3-P_DH_AS.
IPR020829. GlycerAld_3-P_DH_cat.
IPR020828. GlycerAld_3-P_DH_NAD(P)-bd.
IPR006424. Glyceraldehyde-3-P_DH_1.
IPR016040. NAD(P)-bd_dom.
[Graphical view]
PANTHERiPTHR10836. PTHR10836. 1 hit.
PfamiPF02800. Gp_dh_C. 1 hit.
PF00044. Gp_dh_N. 1 hit.
[Graphical view]
PIRSFiPIRSF000149. GAP_DH. 1 hit.
PRINTSiPR00078. G3PDHDRGNASE.
SMARTiSM00846. Gp_dh_N. 1 hit.
[Graphical view]
SUPFAMiSSF51735. SSF51735. 1 hit.
TIGRFAMsiTIGR01534. GAPDH-I. 1 hit.
PROSITEiPS00071. GAPDH. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiG3P_OGAPD
AccessioniPrimary (citable) accession number: O59841
Secondary accession number(s): E7R3B6, W1QK29
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 15, 1999
Last sequence update: May 31, 2011
Last modified: September 7, 2016
This is version 92 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.