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Protein

Hsk1-interacting molecule 1

Gene

him1

Organism
Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Activates hsk1 kinase and is essential for G1/S transition. Has a role in S-phase checkpoint control induced by replication fork blocks after nucleotide deprivation and DNA damage.1 Publication

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri492 – 541DBF4-typePROSITE-ProRule annotationAdd BLAST50

GO - Molecular functioni

  • nucleic acid binding Source: InterPro
  • protein kinase activator activity Source: PomBase
  • protein serine/threonine kinase activator activity Source: PomBase
  • zinc ion binding Source: InterPro

GO - Biological processi

  • cellular response to DNA damage stimulus Source: UniProtKB-KW
  • maintenance of mitotic sister chromatid cohesion, centromeric Source: PomBase
  • mitotic DNA replication checkpoint Source: PomBase
  • negative regulation of DNA binding Source: PomBase
  • peptidyl-serine phosphorylation Source: PomBase
  • positive regulation of DNA replication initiation Source: PomBase
  • positive regulation of protein serine/threonine kinase activity Source: PomBase
  • regulation of mitotic DNA replication initiation Source: PomBase
  • replication fork protection Source: PomBase
Complete GO annotation...

Keywords - Biological processi

Cell cycle, DNA damage

Keywords - Ligandi

Metal-binding, Zinc

Names & Taxonomyi

Protein namesi
Recommended name:
Hsk1-interacting molecule 1
Alternative name(s):
DNA repair protein rad35
Gene namesi
Name:him1
Synonyms:dfp1, rad35
ORF Names:SPCC550.13
OrganismiSchizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Taxonomic identifieri284812 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaTaphrinomycotinaSchizosaccharomycetesSchizosaccharomycetalesSchizosaccharomycetaceaeSchizosaccharomyces
Proteomesi
  • UP000002485 Componenti: Chromosome III

Organism-specific databases

EuPathDBiFungiDB:SPCC550.13.
PomBaseiSPCC550.13.

Subcellular locationi

  • Nucleus 1 Publication

GO - Cellular componenti

  • Dbf4-dependent protein kinase complex Source: PomBase
  • mitotic spindle pole body Source: PomBase
  • nucleus Source: PomBase
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000839781 – 545Hsk1-interacting molecule 1Add BLAST545

Post-translational modificationi

Hyperphosphorylated at the G1/S and S-phases of the cell cycle.

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiO59836.
PRIDEiO59836.

Interactioni

Subunit structurei

Associates with hsk1. Interacts with mcm10.2 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
swi6P403812EBI-908382,EBI-926939

Protein-protein interaction databases

BioGridi275964. 15 interactors.
IntActiO59836. 5 interactors.
MINTiMINT-4676598.

Structurei

3D structure databases

ProteinModelPortaliO59836.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Contains 1 DBF4-type zinc finger.PROSITE-ProRule annotation

Zinc finger

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri492 – 541DBF4-typePROSITE-ProRule annotationAdd BLAST50

Keywords - Domaini

Zinc-finger

Phylogenomic databases

InParanoidiO59836.
KOiK02309.
OMAiESCRVKF.
OrthoDBiEOG092C4W9O.
PhylomeDBiO59836.

Family and domain databases

CDDicd00027. BRCT. 1 hit.
InterProiIPR001357. BRCT_dom.
IPR013939. Regulatory_Dfp1/Him1.
IPR006572. Znf_DBF.
[Graphical view]
PfamiPF08630. Dfp1_Him1_M. 1 hit.
PF07535. zf-DBF. 1 hit.
[Graphical view]
SMARTiSM00292. BRCT. 1 hit.
SM00586. ZnF_DBF. 1 hit.
[Graphical view]
PROSITEiPS51265. ZF_DBF4. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

O59836-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MNLGRCPLAP RSANIVLPKH DAVSKQKEYR IEEKTNEAQR EEIITWKDNR
60 70 80 90 100
EDEGEVKTDF EVVNNENIIT TTPKHQTVIT PKSYRKSVKR IKHDAPQNED
110 120 130 140 150
IPVMKGLAPI NADTESKAES MAAGKVLGSK NSSQKARLQE WKRQYKKAFP
160 170 180 190 200
HFRFYLDGCD PSVAHRVKKQ IQQLGGHVET FFSGNVTHVA TVRAIQDVSV
210 220 230 240 250
KYAKQDVITK ARQLNMKIWS MEKLCNRVLK TLMENDQCTT NAPTKQGNDL
260 270 280 290 300
SYLLYVEKVQ GTNERDLSVP RQDFVHFRGP YLYVHDIANI YKPILLREWQ
310 320 330 340 350
KPLPDRDVPW PTFRATSIGR CPFVPETKYR LSTSKSLVAK NDQQLLQRQS
360 370 380 390 400
QEPSLILRAN SMKASLPDIS NTGISGMNTN TTYNTNINNT PQTAISGITQ
410 420 430 440 450
DTSPSIRTNC HHCLDDGMQA SGIVQSNLTS AAMSNNSAIR SGSAASVPVV
460 470 480 490 500
TINGRDIAEL KKRIIQQKSG MIGKDYSYKA MLHNTSQRKI RVDAKPGYCE
510 520 530 540
NCREKFDNFE SHIRSSRHRR FAENNDNFKD LDELFALVQR PLRPD
Length:545
Mass (Da):61,860
Last modified:August 1, 1998 - v1
Checksum:i96F90243322AFD8D
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF110398 Genomic DNA. Translation: AAD03738.1.
AB028068 mRNA. Translation: BAA78328.1.
Y17146 Genomic DNA. Translation: CAA76653.1.
CU329672 Genomic DNA. Translation: CAA19117.1.
PIRiT43513.
RefSeqiNP_588105.1. NM_001023096.2.

Genome annotation databases

EnsemblFungiiSPCC550.13.1; SPCC550.13.1:pep; SPCC550.13.
GeneIDi2539399.
KEGGispo:SPCC550.13.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF110398 Genomic DNA. Translation: AAD03738.1.
AB028068 mRNA. Translation: BAA78328.1.
Y17146 Genomic DNA. Translation: CAA76653.1.
CU329672 Genomic DNA. Translation: CAA19117.1.
PIRiT43513.
RefSeqiNP_588105.1. NM_001023096.2.

3D structure databases

ProteinModelPortaliO59836.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi275964. 15 interactors.
IntActiO59836. 5 interactors.
MINTiMINT-4676598.

Proteomic databases

MaxQBiO59836.
PRIDEiO59836.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiSPCC550.13.1; SPCC550.13.1:pep; SPCC550.13.
GeneIDi2539399.
KEGGispo:SPCC550.13.

Organism-specific databases

EuPathDBiFungiDB:SPCC550.13.
PomBaseiSPCC550.13.

Phylogenomic databases

InParanoidiO59836.
KOiK02309.
OMAiESCRVKF.
OrthoDBiEOG092C4W9O.
PhylomeDBiO59836.

Miscellaneous databases

PROiO59836.

Family and domain databases

CDDicd00027. BRCT. 1 hit.
InterProiIPR001357. BRCT_dom.
IPR013939. Regulatory_Dfp1/Him1.
IPR006572. Znf_DBF.
[Graphical view]
PfamiPF08630. Dfp1_Him1_M. 1 hit.
PF07535. zf-DBF. 1 hit.
[Graphical view]
SMARTiSM00292. BRCT. 1 hit.
SM00586. ZnF_DBF. 1 hit.
[Graphical view]
PROSITEiPS51265. ZF_DBF4. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiHIM1_SCHPO
AccessioniPrimary (citable) accession number: O59836
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 6, 2002
Last sequence update: August 1, 1998
Last modified: November 30, 2016
This is version 121 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Schizosaccharomyces pombe
    Schizosaccharomyces pombe: entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.