O59828 (ALR1_SCHPO) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Alanine racemase, catabolic
EC=5.1.1.1

Gene names

Name:	alr1
ORF Names:	SPCC965.08c

Organism

Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)

Taxonomic identifier

284812 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Taphrinomycotina › Schizosaccharomycetes › Schizosaccharomycetales › Schizosaccharomycetaceae › Schizosaccharomyces

Protein attributes

Sequence length	375 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Catalytic activity	L-alanine = D-alanine.
Cofactor	Pyridoxal phosphate.
Pathway	Amino-acid biosynthesis; D-alanine biosynthesis; D-alanine from L-alanine: step 1/1.
Sequence similarities	Belongs to the alanine racemase family.
Biophysicochemical properties	pH dependence: Optimum pH is 9.0-9.5.

Ontologies

Keywords
Ligand	Pyridoxal phosphate
Molecular function	Isomerase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	alanine metabolic process Inferred from direct assay. Source: GeneDB_Spombe
Cellular component	cytosol Inferred from direct assay. Source: GeneDB_Spombe nucleus Inferred from direct assay. Source: GeneDB_Spombe
Molecular function	alanine racemase activity Inferred from direct assay. Source: GeneDB_Spombe serine racemase activity Inferred from direct assay. Source: GeneDB_Spombe
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 375	375	Alanine racemase, catabolic		PRO_0000114604
Sites
	Active site	38	1	Proton acceptor; specific for D-alanine By similarity
	Active site	269	1	Proton acceptor; specific for L-alanine By similarity
Amino acid modifications
	Modified residue	38	1	N6-(pyridoxal phosphate)lysine By similarity

Sequences

	Sequence		Length	Mass (Da)
	O59828 [UniParc]. Last modified August 1, 1998. Version 1. Checksum: 76CEBD17E159E13F	FASTA	375	41,585
	10 20 30 40 50 60 MRGAKSVIDL HAIAHNYNVA KQMMLQKNPS GHVLAIVKAN AYGHGAVQVA RFLLKHCSSI 70 80 90 100 110 120 DGFGVSSIEE ALELRHGGIY NKIVLLEGFF TEEDELKLID DYNFSIIIHS EDQVNSFIKY 130 140 150 160 170 180 PFNRPVEIWL KLDSGMNRLG FTPSQFMKFY NLLSNNKNVS NIGKITHFAF ADMLENPEHT 190 200 210 220 230 240 LKQWDIFEKS VAHLPGPLSA GGSAIILGWL NTVCTDWLRA GIMLYGISPF LSKNKDSKTP 250 260 270 280 290 300 ESVNIKPAMK LVSTIISVKH VDKGQPIGYG GRYVATRDMK LGVVAMGYGD GFPRQVKDGC 310 320 330 340 350 360 PVLVNGVKAP IVGRVSMDML TVDLSDIPDV KPGDEVIFWG TPELTVADIA KYCSDTSPYE 370 LVTKLTRRVP LQYTY

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The genome sequence of Schizosaccharomyces pombe." Wood V., Gwilliam R., Rajandream M.A., Lyne M.H., Lyne R., Stewart A., Sgouros J.G., Peat N., Hayles J., Baker S.G., Basham D., Bowman S., Brooks K., Brown D., Brown S., Chillingworth T., Churcher C.M., Collins M. , Connor R., Cronin A., Davis P., Feltwell T., Fraser A., Gentles S., Goble A., Hamlin N., Harris D.E., Hidalgo J., Hodgson G., Holroyd S., Hornsby T., Howarth S., Huckle E.J., Hunt S., Jagels K., James K.D., Jones L., Jones M., Leather S., McDonald S., McLean J., Mooney P., Moule S., Mungall K.L., Murphy L.D., Niblett D., Odell C., Oliver K., O'Neil S., Pearson D., Quail M.A., Rabbinowitsch E., Rutherford K.M., Rutter S., Saunders D., Seeger K., Sharp S., Skelton J., Simmonds M.N., Squares R., Squares S., Stevens K., Taylor K., Taylor R.G., Tivey A., Walsh S.V., Warren T., Whitehead S., Woodward J.R., Volckaert G., Aert R., Robben J., Grymonprez B., Weltjens I., Vanstreels E., Rieger M., Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Duesterhoeft A., Fritzc C., Holzer E., Moestl D., Hilbert H., Borzym K., Langer I., Beck A., Lehrach H., Reinhardt R., Pohl T.M., Eger P., Zimmermann W., Wedler H., Wambutt R., Purnelle B., Goffeau A., Cadieu E., Dreano S., Gloux S., Lelaure V., Mottier S., Galibert F., Aves S.J., Xiang Z., Hunt C., Moore K., Hurst S.M., Lucas M., Rochet M., Gaillardin C., Tallada V.A., Garzon A., Thode G., Daga R.R., Cruzado L., Jimenez J., Sanchez M., del Rey F., Benito J., Dominguez A., Revuelta J.L., Moreno S., Armstrong J., Forsburg S.L., Cerutti L., Lowe T., McCombie W.R., Paulsen I., Potashkin J., Shpakovski G.V., Ussery D., Barrell B.G., Nurse P. Nature 415:871-880(2002) [PubMed: 11859360] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: 972 / ATCC 24843.
[2]	"Functional characterization of alanine racemase from Schizosaccharomyces pombe: a eucaryotic counterpart to bacterial alanine racemase." Uo T., Yoshimura T., Tanaka N., Takegawa K., Esaki N. J. Bacteriol. 183:2226-2233(2001) [PubMed: 11244061] [Abstract] Cited for: CHARACTERIZATION. Strain: SP1.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CU329672 Genomic DNA. Translation: CAA19068.1.
PIR	T41661.
RefSeq	NP_588518.1. NM_001023507.1.
3D structure databases
ProteinModelPortal	O59828.
ModBase	Search...
Protein-protein interaction databases
STRING	O59828.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	SPCC965.08c.1; SPCC965.08c.1:pep; SPCC965.08c.
GeneID	2538742.
GenomeReviews	Gene locus alr1 in contig CU329672_GR.
KEGG	spo:SPCC965.08c.
NMPDR	fig|4896.1.peg.856.
Organism-specific databases
GeneDB_Spombe	SPCC965.08c.
Phylogenomic databases
eggNOG	COG0787.
GeneTree	EFGT00050000008702.
HOGENOM	HBG712172.
OMA	ATAYINT.
Enzyme and pathway databases
BioCyc	SPOM-XXX-01:SPOM-XXX-01-000419-MONOMER.
BRENDA	5.1.1.1. 5615.
Gene expression databases
ArrayExpress	O59828.
Family and domain databases
InterPro	IPR000821. Ala_racemase. IPR009006. Ala_racemase/Decarboxylase_C. IPR011079. Ala_racemase_C. IPR001608. Ala_racemase_N. IPR020622. Ala_racemase_pyridoxalP-BS. [Graphical view]
Gene3D	G3DSA:2.40.37.10. Ala_racemase/Decarboxylase_C. 1 hit.
KO	K01775.
Pfam	PF00842. Ala_racemase_C. 1 hit. PF01168. Ala_racemase_N. 1 hit. [Graphical view]
PRINTS	PR00992. ALARACEMASE.
SMART	SM01005. Ala_racemase_C. 1 hit. [Graphical view]
SUPFAM	SSF50621. Racem_decarbox_C. 1 hit.
TIGRFAMs	TIGR00492. Alr. 1 hit.
PROSITE	PS00395. ALANINE_RACEMASE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

ALR1_SCHPO

Accession

Primary (citable) accession number: O59828

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 11, 2001
Last sequence update:	August 1, 1998
Last modified:	January 25, 2012
This is version 81 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program