Reviewed, UniProtKB/Swiss-Prot O59802 (GPI2_SCHPO)

Names and origin

Protein names

Recommended name:
    Phosphatidylinositol N-acetylglucosaminyltransferase GPI2 subunit
    EC=2.4.1.198

Gene names

Name:	gpi2
ORF Names:	SPCC550.04c

Organism

Schizosaccharomyces pombe (Fission yeast) [Complete proteome]

Taxonomic identifier

4896 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Taphrinomycotina › Schizosaccharomycetes › Schizosaccharomycetales › Schizosaccharomycetaceae › Schizosaccharomyces

Protein attributes

Sequence length	324 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Part of the complex catalyzing the transfer of N-acetylglucosamine from UDP-N-acetylglucosamine to phosphatidylinositol, the first step of GPI biosynthesis.
Catalytic activity	UDP-N-acetyl-D-glucosamine + 1-phosphatidyl-1D-myo-inositol = UDP + 6-(N-acetyl-alpha-D-glucosaminyl)-1-phosphatidyl-1D-myo-inositol.
Pathway	Glycolipid biosynthesis; glycosylphosphatidylinositol-anchor biosynthesis.
Subcellular location	Endoplasmic reticulum membrane; Multi-pass membrane protein. Ref.2
Sequence similarities	Belongs to the PIGC family.

Ontologies

Keywords
Biological process	GPI-anchor biosynthesis
Cellular component	Endoplasmic reticulum Membrane
Domain	Transmembrane
Molecular function	Glycosyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	GPI anchor biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	endoplasmic reticulum Ref.2 Inferred from direct assay. Source: GeneDB_SPombe integral to membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	phosphatidylinositol N-acetylglucosaminyltransferase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 324	324	Phosphatidylinositol N-acetylglucosaminyltransferase GPI2 subunit		PRO_0000372382
Regions
	Topological domain	1 – 79	79	Cytoplasmic Potential
	Transmembrane	80 – 100	21	Potential
	Topological domain	101 – 107	7	Lumenal Potential
	Transmembrane	108 – 128	21	Potential
	Topological domain	129 – 140	12	Cytoplasmic Potential
	Transmembrane	141 – 161	21	Potential
	Topological domain	162 – 171	10	Lumenal Potential
	Transmembrane	172 – 192	21	Potential
	Topological domain	193 – 223	31	Cytoplasmic Potential
	Transmembrane	224 – 244	21	Potential
	Topological domain	245 – 250	6	Lumenal Potential
	Transmembrane	251 – 271	21	Potential
	Topological domain	272 – 276	5	Cytoplasmic Potential
	Transmembrane	277 – 299	23	Potential
	Topological domain	300 – 324	25	Lumenal Potential

Sequences

	Sequence		Length	Mass (Da)
	O59802-1 [UniParc]. Last modified August 1, 1998. Version 1. Checksum: 7C14AB3EA9138DD1	FASTA	324	36,654
	10 20 30 40 50 60 MDEVCAAPAP KKMVAKSLVL NTFRKASVKE VVELKKPWKK VLWRKQDYPD NFIDESFLNG 70 80 90 100 110 120 LQRNVNIQVT DFWSLVADSL PVSQHLSSVV IFASVFVSIY RNQLSCALVG FVSNVSAVAA 130 140 150 160 170 180 FILWDFVLRK PCNNRTFPNY MGIVKSCILI VLTLAGLSPI LMSLTKSTSP DSVWAIAVWL 190 200 210 220 230 240 FLANVFFHEY TTETIRPHVR LHNSLSTNAA LSASVVLASR LEKSINVFFF ILFAVHWFAL 250 260 270 280 290 300 FPIFRKYIHV FSFYADMLMT LVLIISAYIA LNAVASVVIA FVFLSLIFFI SFICPIWFIK 310 320 LQRFKNEIHG PWDIALPKLG PSKG

References

[1]

"The genome sequence of Schizosaccharomyces pombe." Wood V., Gwilliam R., Rajandream M.A., Lyne M.H., Lyne R., Stewart A., Sgouros J.G., Peat N., Hayles J., Baker S.G., Basham D., Bowman S., Brooks K., Brown D., Brown S., Chillingworth T., Churcher C.M., Collins M. , Connor R., Cronin A., Davis P., Feltwell T., Fraser A., Gentles S., Goble A., Hamlin N., Harris D.E., Hidalgo J., Hodgson G., Holroyd S., Hornsby T., Howarth S., Huckle E.J., Hunt S., Jagels K., James K.D., Jones L., Jones M., Leather S., McDonald S., McLean J., Mooney P., Moule S., Mungall K.L., Murphy L.D., Niblett D., Odell C., Oliver K., O'Neil S., Pearson D., Quail M.A., Rabbinowitsch E., Rutherford K.M., Rutter S., Saunders D., Seeger K., Sharp S., Skelton J., Simmonds M.N., Squares R., Squares S., Stevens K., Taylor K., Taylor R.G., Tivey A., Walsh S.V., Warren T., Whitehead S., Woodward J.R., Volckaert G., Aert R., Robben J., Grymonprez B., Weltjens I., Vanstreels E., Rieger M., Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Duesterhoeft A., Fritzc C., Holzer E., Moestl D., Hilbert H., Borzym K., Langer I., Beck A., Lehrach H., Reinhardt R., Pohl T.M., Eger P., Zimmermann W., Wedler H., Wambutt R., Purnelle B., Goffeau A., Cadieu E., Dreano S., Gloux S., Lelaure V., Mottier S., Galibert F., Aves S.J., Xiang Z., Hunt C., Moore K., Hurst S.M., Lucas M., Rochet M., Gaillardin C., Tallada V.A., Garzon A., Thode G., Daga R.R., Cruzado L., Jimenez J., Sanchez M., del Rey F., Benito J., Dominguez A., Revuelta J.L., Moreno S., Armstrong J., Forsburg S.L., Cerutti L., Lowe T., McCombie W.R., Paulsen I., Potashkin J., Shpakovski G.V., Ussery D., Barrell B.G., Nurse P. Nature 415:871-880(2002) [PubMed: 11859360] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 38366 / 972.

[2]

"ORFeome cloning and global analysis of protein localization in the fission yeast Schizosaccharomyces pombe." Matsuyama A., Arai R., Yashiroda Y., Shirai A., Kamata A., Sekido S., Kobayashi Y., Hashimoto A., Hamamoto M., Hiraoka Y., Horinouchi S., Yoshida M. Nat. Biotechnol. 24:841-847(2006) [PubMed: 16823372] [Abstract] Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].

Cross-references

Sequence databases
EMBLGenBankDDBJ	CU329672 Genomic DNA. Translation: CAA19108.1.
PIR	T41379.
RefSeq	NP_588096.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	O59802.
Genome annotation databases
GeneID	2539262.
GenomeReviews	Gene locus gpi2 in contig CU329672_GR.
KEGG	spo:SPCC550.04c.
NMPDR	fig|4896.1.peg.434.
Organism-specific databases
GeneDB_Spombe	SPCC550.04c.
Phylogenomic databases
OMA	VVIQQLC.
Gene expression databases
ArrayExpress	O59802.
Family and domain databases
InterPro	IPR009450. Plno_GlcNAc_GPI2. [Graphical view]
PANTHER	PTHR12982. GPI2. 1 hit.
Pfam	PF06432. GPI2. 1 hit. [Graphical view]
PIRSF	PIRSF016104. GPI2. 1 hit.
ProtoNet	Search...

Entry information

Entry name

GPI2_SCHPO

Accession

Primary (citable) accession number: O59802

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 5, 2009
Last sequence update:	August 1, 1998
Last modified:	November 3, 2009
This is version 39 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

FPAP (Fungal Proteome Annotation Project)