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Protein

Pre-mRNA-splicing factor cwf5

Gene

cwf5

Organism
Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Facilitates the cooperative formation of U2/U6 helix II in association with stem II in the spliceosome. Binds to RNA (By similarity). Involved in pre-mRNA splicing.By similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Biological processi

mRNA processing, mRNA splicing

Keywords - Ligandi

RNA-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Pre-mRNA-splicing factor cwf5
Alternative name(s):
Complexed with cdc5 protein 5
Gene namesi
Name:cwf5
Synonyms:ecm2
ORF Names:SPCC550.02c
OrganismiSchizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Taxonomic identifieri284812 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaTaphrinomycotinaSchizosaccharomycetesSchizosaccharomycetalesSchizosaccharomycetaceaeSchizosaccharomyces
Proteomesi
  • UP000002485 Componenti: Chromosome III

Organism-specific databases

EuPathDBiFungiDB:SPCC550.02c.
PomBaseiSPCC550.02c. cwf5.

Subcellular locationi

GO - Cellular componenti

  • nucleus Source: PomBase
  • Prp19 complex Source: PomBase
  • spliceosomal complex Source: PomBase
Complete GO annotation...

Keywords - Cellular componenti

Nucleus, Spliceosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 354354Pre-mRNA-splicing factor cwf5PRO_0000081553Add
BLAST

Proteomic databases

MaxQBiO59800.
PRIDEiO59800.

Interactioni

Subunit structurei

Belongs to the 40S cdc5-associated complex (or cwf complex), a spliceosome sub-complex reminiscent of a late-stage spliceosome composed of the U2, U5 and U6 snRNAs and at least brr2, cdc5, cwf2/prp3, cwf3/syf1, cwf4/syf3, cwf5/ecm2, spp42/cwf6, cwf7/spf27, cwf8, cwf9, cwf10, cwf11, cwf12, prp45/cwf13, cwf14, cwf15, cwf16, cwf17, cwf18, cwf19, cwf20, cwf21, cwf22, cwf23, cwf24, cwf25, cwf26, cyp7/cwf27, cwf28, cwf29/ist3, lea1, msl1, prp5/cwf1, prp10, prp12/sap130, prp17, prp22, sap61, sap62, sap114, sap145, slu7, smb1, smd1, smd3, smf1, smg1 and syf2.1 Publication

Binary interactionsi

WithEntry#Exp.IntActNotes
cdc5P399644EBI-538826,EBI-538771

Protein-protein interaction databases

BioGridi275548. 28 interactions.
IntActiO59800. 6 interactions.
MINTiMINT-4676268.

Structurei

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
3JB9electron microscopy3.60a1-151[»]
a178-285[»]
ProteinModelPortaliO59800.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini212 – 28675RRMPROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Belongs to the RRM SLT11 family.Curated
Contains 1 RRM (RNA recognition motif) domain.PROSITE-ProRule annotation

Phylogenomic databases

HOGENOMiHOG000171086.
InParanoidiO59800.
KOiK12872.
OMAiEFGRSCG.
OrthoDBiEOG7SFJ6P.
PhylomeDBiO59800.

Family and domain databases

Gene3Di3.30.70.330. 2 hits.
InterProiIPR012677. Nucleotide-bd_a/b_plait.
IPR000504. RRM_dom.
[Graphical view]
PfamiPF00076. RRM_1. 1 hit.
[Graphical view]
SMARTiSM00360. RRM. 1 hit.
[Graphical view]
SUPFAMiSSF54928. SSF54928. 1 hit.
PROSITEiPS50102. RRM. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

O59800-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSHGPKGDIN KQEWEDVEFP SICERCLGDN SYVRMTKEPL GQECKICSKP
60 70 80 90 100
CTIFRWIKER GQKPGKTQIC VGCARYKNCC QSCMLDLQFG LPIALRDAAL
110 120 130 140 150
KLVESGPTND INREFFAQNQ QRLLSNGETA YDSQEASAAA RNLVKKVEKR
160 170 180 190 200
ELHSRPPKRK LDDVESKQIL KEARASDASL NAERPLFPVK KIINGNVSLS
210 220 230 240 250
INMEPPKDKK IASLFLIGVE DELADYKIRK HFEQYGPLKS VVCSHRAKCA
260 270 280 290 300
FVNFKTRSSA EIAAAASPDG NVVIEGFRLK VQWGKPRSLG GPEGEVRNAK
310 320 330 340 350
LADLVMRGSS HGNKTSQKST IKNIENEDHE NTKSPAVAIP IDPNQPRYRS

QIPR
Length:354
Mass (Da):39,576
Last modified:August 1, 1998 - v1
Checksum:i5283C11C4BD504AA
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF254352 Genomic DNA. Translation: AAF67751.1.
CU329672 Genomic DNA. Translation: CAA19106.1.
AB027880 Genomic DNA. Translation: BAA87184.1.
PIRiT41377.
RefSeqiNP_588094.1. NM_001023085.1.

Genome annotation databases

EnsemblFungiiSPCC550.02c.1; SPCC550.02c.1:pep; SPCC550.02c.
GeneIDi2538974.
KEGGispo:SPCC550.02c.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF254352 Genomic DNA. Translation: AAF67751.1.
CU329672 Genomic DNA. Translation: CAA19106.1.
AB027880 Genomic DNA. Translation: BAA87184.1.
PIRiT41377.
RefSeqiNP_588094.1. NM_001023085.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
3JB9electron microscopy3.60a1-151[»]
a178-285[»]
ProteinModelPortaliO59800.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi275548. 28 interactions.
IntActiO59800. 6 interactions.
MINTiMINT-4676268.

Proteomic databases

MaxQBiO59800.
PRIDEiO59800.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiSPCC550.02c.1; SPCC550.02c.1:pep; SPCC550.02c.
GeneIDi2538974.
KEGGispo:SPCC550.02c.

Organism-specific databases

EuPathDBiFungiDB:SPCC550.02c.
PomBaseiSPCC550.02c. cwf5.

Phylogenomic databases

HOGENOMiHOG000171086.
InParanoidiO59800.
KOiK12872.
OMAiEFGRSCG.
OrthoDBiEOG7SFJ6P.
PhylomeDBiO59800.

Miscellaneous databases

NextBioi20800149.
PROiO59800.

Family and domain databases

Gene3Di3.30.70.330. 2 hits.
InterProiIPR012677. Nucleotide-bd_a/b_plait.
IPR000504. RRM_dom.
[Graphical view]
PfamiPF00076. RRM_1. 1 hit.
[Graphical view]
SMARTiSM00360. RRM. 1 hit.
[Graphical view]
SUPFAMiSSF54928. SSF54928. 1 hit.
PROSITEiPS50102. RRM. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Myb-related fission yeast cdc5p is a component of a 40S snRNP-containing complex and is essential for pre-mRNA splicing."
    McDonald W.H., Ohi R., Smelkova N., Frendewey D., Gould K.L.
    Mol. Cell. Biol. 19:5352-5362(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 211-227 AND 258-278.
  2. "The genome sequence of Schizosaccharomyces pombe."
    Wood V., Gwilliam R., Rajandream M.A., Lyne M.H., Lyne R., Stewart A., Sgouros J.G., Peat N., Hayles J., Baker S.G., Basham D., Bowman S., Brooks K., Brown D., Brown S., Chillingworth T., Churcher C.M., Collins M.
    , Connor R., Cronin A., Davis P., Feltwell T., Fraser A., Gentles S., Goble A., Hamlin N., Harris D.E., Hidalgo J., Hodgson G., Holroyd S., Hornsby T., Howarth S., Huckle E.J., Hunt S., Jagels K., James K.D., Jones L., Jones M., Leather S., McDonald S., McLean J., Mooney P., Moule S., Mungall K.L., Murphy L.D., Niblett D., Odell C., Oliver K., O'Neil S., Pearson D., Quail M.A., Rabbinowitsch E., Rutherford K.M., Rutter S., Saunders D., Seeger K., Sharp S., Skelton J., Simmonds M.N., Squares R., Squares S., Stevens K., Taylor K., Taylor R.G., Tivey A., Walsh S.V., Warren T., Whitehead S., Woodward J.R., Volckaert G., Aert R., Robben J., Grymonprez B., Weltjens I., Vanstreels E., Rieger M., Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Duesterhoeft A., Fritzc C., Holzer E., Moestl D., Hilbert H., Borzym K., Langer I., Beck A., Lehrach H., Reinhardt R., Pohl T.M., Eger P., Zimmermann W., Wedler H., Wambutt R., Purnelle B., Goffeau A., Cadieu E., Dreano S., Gloux S., Lelaure V., Mottier S., Galibert F., Aves S.J., Xiang Z., Hunt C., Moore K., Hurst S.M., Lucas M., Rochet M., Gaillardin C., Tallada V.A., Garzon A., Thode G., Daga R.R., Cruzado L., Jimenez J., Sanchez M., del Rey F., Benito J., Dominguez A., Revuelta J.L., Moreno S., Armstrong J., Forsburg S.L., Cerutti L., Lowe T., McCombie W.R., Paulsen I., Potashkin J., Shpakovski G.V., Ussery D., Barrell B.G., Nurse P.
    Nature 415:871-880(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: 972 / ATCC 24843.
  3. "Large-scale screening of intracellular protein localization in living fission yeast cells by the use of a GFP-fusion genomic DNA library."
    Ding D.-Q., Tomita Y., Yamamoto A., Chikashige Y., Haraguchi T., Hiraoka Y.
    Genes Cells 5:169-190(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA] OF 212-328, SUBCELLULAR LOCATION.
    Strain: ATCC 38364 / 968.
  4. "Proteomics analysis reveals stable multiprotein complexes in both fission and budding yeasts containing Myb-related Cdc5p/Cef1p, novel pre-mRNA splicing factors, and snRNAs."
    Ohi M.D., Link A.J., Ren L., Jennings J.L., McDonald W.H., Gould K.L.
    Mol. Cell. Biol. 22:2011-2024(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION IN THE CWF COMPLEX, IDENTIFICATION BY MASS SPECTROMETRY.

Entry informationi

Entry nameiSLT11_SCHPO
AccessioniPrimary (citable) accession number: O59800
Secondary accession number(s): Q9USC0
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 29, 2001
Last sequence update: August 1, 1998
Last modified: May 11, 2016
This is version 128 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. Schizosaccharomyces pombe
    Schizosaccharomyces pombe: entries and gene names
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.