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Protein

Pre-mRNA-splicing factor cwf5

Gene

cwf5

Organism
Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Facilitates the cooperative formation of U2/U6 helix II in association with stem II in the spliceosome. Binds to RNA (By similarity). Involved in pre-mRNA splicing.By similarity

GO - Molecular functioni

GO - Biological processi

  • mRNA cis splicing, via spliceosome Source: PomBase
  • spliceosomal snRNP assembly Source: GO_Central

Keywordsi

Molecular functionRNA-binding
Biological processmRNA processing, mRNA splicing

Names & Taxonomyi

Protein namesi
Recommended name:
Pre-mRNA-splicing factor cwf5
Alternative name(s):
Complexed with cdc5 protein 5
Gene namesi
Name:cwf5
Synonyms:ecm2
ORF Names:SPCC550.02c
OrganismiSchizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Taxonomic identifieri284812 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaTaphrinomycotinaSchizosaccharomycetesSchizosaccharomycetalesSchizosaccharomycetaceaeSchizosaccharomyces
Proteomesi
  • UP000002485 Componenti: Chromosome III

Organism-specific databases

EuPathDBiFungiDB:SPCC550.02c
PomBaseiSPCC550.02c cwf5

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Nucleus, Spliceosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000815531 – 354Pre-mRNA-splicing factor cwf5Add BLAST354

Proteomic databases

MaxQBiO59800
PaxDbiO59800
PRIDEiO59800

Interactioni

Subunit structurei

Belongs to the 40S cdc5-associated complex (or cwf complex), a spliceosome sub-complex reminiscent of a late-stage spliceosome composed of the U2, U5 and U6 snRNAs and at least brr2, cdc5, cwf2/prp3, cwf3/syf1, cwf4/syf3, cwf5/ecm2, spp42/cwf6, cwf7/spf27, cwf8, cwf9, cwf10, cwf11, cwf12, prp45/cwf13, cwf14, cwf15, cwf16, cwf17, cwf18, cwf19, cwf20, cwf21, cwf22, cwf23, cwf24, cwf25, cwf26, cyp7/cwf27, cwf28, cwf29/ist3, lea1, msl1, prp5/cwf1, prp10, prp12/sap130, prp17, prp22, sap61, sap62, sap114, sap145, slu7, smb1, smd1, smd3, smf1, smg1 and syf2.1 Publication

Binary interactionsi

WithEntry#Exp.IntActNotes
cdc5P399644EBI-538826,EBI-538771

Protein-protein interaction databases

BioGridi275548, 28 interactors
IntActiO59800, 6 interactors
STRINGi4896.SPCC550.02c.1

Structurei

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3JB9electron microscopy3.60a1-285[»]
ProteinModelPortaliO59800
SMRiO59800
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini212 – 286RRMPROSITE-ProRule annotationAdd BLAST75

Sequence similaritiesi

Belongs to the RRM SLT11 family.Curated

Phylogenomic databases

HOGENOMiHOG000171086
InParanoidiO59800
KOiK12872
OMAiLCTRPFT
OrthoDBiEOG092C3DPH
PhylomeDBiO59800

Family and domain databases

CDDicd12265 RRM_SLT11, 1 hit
Gene3Di3.30.70.330, 1 hit
InterProiView protein in InterPro
IPR012677 Nucleotide-bd_a/b_plait_sf
IPR035979 RBD_domain_sf
IPR000504 RRM_dom
IPR034356 Slt11-like_RRM
PfamiView protein in Pfam
PF00076 RRM_1, 1 hit
SMARTiView protein in SMART
SM00360 RRM, 1 hit
SUPFAMiSSF54928 SSF54928, 1 hit
PROSITEiView protein in PROSITE
PS50102 RRM, 1 hit

Sequencei

Sequence statusi: Complete.

O59800-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSHGPKGDIN KQEWEDVEFP SICERCLGDN SYVRMTKEPL GQECKICSKP
60 70 80 90 100
CTIFRWIKER GQKPGKTQIC VGCARYKNCC QSCMLDLQFG LPIALRDAAL
110 120 130 140 150
KLVESGPTND INREFFAQNQ QRLLSNGETA YDSQEASAAA RNLVKKVEKR
160 170 180 190 200
ELHSRPPKRK LDDVESKQIL KEARASDASL NAERPLFPVK KIINGNVSLS
210 220 230 240 250
INMEPPKDKK IASLFLIGVE DELADYKIRK HFEQYGPLKS VVCSHRAKCA
260 270 280 290 300
FVNFKTRSSA EIAAAASPDG NVVIEGFRLK VQWGKPRSLG GPEGEVRNAK
310 320 330 340 350
LADLVMRGSS HGNKTSQKST IKNIENEDHE NTKSPAVAIP IDPNQPRYRS

QIPR
Length:354
Mass (Da):39,576
Last modified:August 1, 1998 - v1
Checksum:i5283C11C4BD504AA
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF254352 Genomic DNA Translation: AAF67751.1
CU329672 Genomic DNA Translation: CAA19106.1
AB027880 Genomic DNA Translation: BAA87184.1
PIRiT41377
RefSeqiNP_588094.1, NM_001023085.1

Genome annotation databases

EnsemblFungiiSPCC550.02c.1; SPCC550.02c.1:pep; SPCC550.02c
GeneIDi2538974
KEGGispo:SPCC550.02c

Similar proteinsi

Entry informationi

Entry nameiSLT11_SCHPO
AccessioniPrimary (citable) accession number: O59800
Secondary accession number(s): Q9USC0
Entry historyiIntegrated into UniProtKB/Swiss-Prot: August 29, 2001
Last sequence update: August 1, 1998
Last modified: March 28, 2018
This is version 144 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome
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Main funding by: National Institutes of Health