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Protein

Serine/threonine-protein kinase ark1

Gene

ark1

Organism
Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Required for the spindle checkpoint attachment response during spindle formation, kinetochore microtubule interactions and chromosome segregation during anaphase. Ark1 activity depends upon cut17 function and phosphorylation. Ark1 with bir1 is required for full-scale association with kinetochores and formation of a complex with mad3. Ark1 is also required for phosphorylation of histone H3 that accompanies chromosome condensation and condensin recruitment to mitotic chromatin. Ark1 with pic1 is required for the execution of cytokinesis.3 Publications

Catalytic activityi

ATP + a protein = ADP + a phosphoprotein.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei118ATPCurated1
Active sitei212Proton acceptorPROSITE-ProRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi95 – 103ATPPROSITE-ProRule annotation9

GO - Molecular functioni

  • ATP binding Source: UniProtKB-KW
  • histone kinase activity (H3-S10 specific) Source: PomBase
  • protein serine/threonine kinase activity Source: PomBase

GO - Biological processi

  • attachment of spindle microtubules to kinetochore involved in meiotic chromosome segregation Source: PomBase
  • correction of merotelic kinetochore attachment, meiosis I Source: PomBase
  • correction of merotelic kinetochore attachment, mitotic Source: PomBase
  • meiotic spindle assembly checkpoint Source: PomBase
  • mitotic chromosome condensation Source: PomBase
  • mitotic cytokinesis Source: PomBase
  • mitotic sister chromatid biorientation Source: PomBase
  • mitotic sister chromatid segregation Source: PomBase
  • mitotic spindle assembly checkpoint Source: PomBase
  • mitotic spindle organization Source: GO_Central
  • positive regulation of G2/M transition of mitotic cell cycle Source: PomBase
  • positive regulation of mitotic cell cycle spindle assembly checkpoint Source: PomBase
  • protein localization to kinetochore Source: PomBase
  • protein localization to nucleolar rDNA repeats Source: PomBase
  • regulation of cytokinesis Source: GO_Central

Keywordsi

Molecular functionKinase, Serine/threonine-protein kinase, Transferase
LigandATP-binding, Nucleotide-binding

Enzyme and pathway databases

BRENDAi2.7.11.1. 5613.
ReactomeiR-SPO-8854050. FBXL7 down-regulates AURKA during mitotic entry and in early mitosis.

Names & Taxonomyi

Protein namesi
Recommended name:
Serine/threonine-protein kinase ark1 (EC:2.7.11.1)
Alternative name(s):
Aurora-related kinase 1
Gene namesi
Name:ark1
Synonyms:sex1
ORF Names:SPCC320.13c, SPCC330.16
OrganismiSchizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Taxonomic identifieri284812 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaTaphrinomycotinaSchizosaccharomycetesSchizosaccharomycetalesSchizosaccharomycetaceaeSchizosaccharomyces
Proteomesi
  • UP000002485 Componenti: Chromosome III

Organism-specific databases

EuPathDBiFungiDB:SPCC320.13c.
PomBaseiSPCC320.13c. ark1.

Subcellular locationi

GO - Cellular componenti

  • chromosome, centromeric region Source: PomBase
  • chromosome passenger complex Source: PomBase
  • condensed chromosome inner kinetochore Source: PomBase
  • condensed nuclear chromosome kinetochore Source: PomBase
  • cytoplasm Source: UniProtKB-KW
  • meiotic spindle Source: PomBase
  • meiotic spindle midzone Source: PomBase
  • mitotic spindle midzone Source: PomBase
  • spindle microtubule Source: GO_Central

Keywords - Cellular componenti

Cytoplasm, Cytoskeleton, Nucleus

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi118K → R: No phosphorylation of bir1; inhibits cytokinesis in a dominant negative manner. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000856341 – 355Serine/threonine-protein kinase ark1Add BLAST355

Proteomic databases

MaxQBiO59790.
PRIDEiO59790.

PTM databases

SwissPalmiO59790.

Interactioni

Subunit structurei

Interacts with bir1 and mad3 to form part of the mad2 complex involved in checkpoint activation. Interacts with pic1.3 Publications

Protein-protein interaction databases

BioGridi280112. 42 interactors.
DIPiDIP-44220N.
IntActiO59790. 2 interactors.
MINTiMINT-4782749.
STRINGi4896.SPCC320.13c.1.

Structurei

3D structure databases

ProteinModelPortaliO59790.
SMRiO59790.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini89 – 340Protein kinasePROSITE-ProRule annotationAdd BLAST252

Sequence similaritiesi

Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. Aurora subfamily.PROSITE-ProRule annotation

Phylogenomic databases

HOGENOMiHOG000233016.
InParanoidiO59790.
KOiK08850.
OMAiQATSVPH.
OrthoDBiEOG092C0QJU.
PhylomeDBiO59790.

Family and domain databases

InterProiView protein in InterPro
IPR030616. Aur.
IPR011009. Kinase-like_dom.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR008271. Ser/Thr_kinase_AS.
PANTHERiPTHR24350. PTHR24350. 1 hit.
PfamiView protein in Pfam
PF00069. Pkinase. 1 hit.
SMARTiView protein in SMART
SM00220. S_TKc. 1 hit.
SUPFAMiSSF56112. SSF56112. 1 hit.
PROSITEiView protein in PROSITE
PS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.

Sequencei

Sequence statusi: Complete.

O59790-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSDSKLADSL NCLSVSTPST TANPGRQQLL RLAVSNQRQV NNVSLANGKE
60 70 80 90 100
NKRTSNSKFN SSLRKIEEPI AGVPSSAGPQ WREFHIGMFE IGKPLGKGKF
110 120 130 140 150
GRVYLAKEKK TGFIVALKTL HKSELVQSKI EKQVRREIEI QSNLRHKNIL
160 170 180 190 200
RLYGHFHDEK RIYLILEFAG RGELYQHLRR AKRFSEEVAS KYIFQMANAL
210 220 230 240 250
SYLHKKHVIH RDIKPENILL GIDGEIKLSD FGWSVHAPSN RRTTLCGTLD
260 270 280 290 300
YLPPEMVEGK EHTEKVDLWS LGVLTYEFLV GAPPFEDMSG HSATYKRIAK
310 320 330 340 350
VDLKIPSFVP PDARDLISRL LQHNPEKRMS LEQVMRHPWI VKYKDSWTRK

SSESS
Length:355
Mass (Da):40,597
Last modified:April 13, 2004 - v2
Checksum:iBF15AC7B7571A301
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CU329672 Genomic DNA. Translation: CAD88263.1.
PIRiT41298.
RefSeqiNP_001018849.1. NM_001022711.2.

Genome annotation databases

EnsemblFungiiSPCC320.13c.1; SPCC320.13c.1:pep; SPCC320.13c.
GeneIDi3361036.
KEGGispo:SPCC320.13c.

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.

Entry informationi

Entry nameiARK1_SCHPO
AccessioniPrimary (citable) accession number: O59790
Entry historyiIntegrated into UniProtKB/Swiss-Prot: June 20, 2002
Last sequence update: April 13, 2004
Last modified: July 5, 2017
This is version 144 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Schizosaccharomyces pombe
    Schizosaccharomyces pombe: entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families