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Protein

Serine/threonine-protein kinase ark1

Gene

ark1

Organism
Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Required for the spindle checkpoint attachment response during spindle formation, kinetochore microtubule interactions and chromosome segregation during anaphase. Ark1 activity depends upon cut17 function and phosphorylation. Ark1 with bir1 is required for full-scale association with kinetochores and formation of a complex with mad3. Ark1 is also required for phosphorylation of histone H3 that accompanies chromosome condensation and condensin recruitment to mitotic chromatin. Ark1 with pic1 is required for the execution of cytokinesis.3 Publications

Catalytic activityi

ATP + a protein = ADP + a phosphoprotein.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei118 – 1181ATPCurated
Active sitei212 – 2121Proton acceptorPROSITE-ProRule annotation

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi95 – 1039ATPPROSITE-ProRule annotation

GO - Molecular functioni

  • ATP binding Source: UniProtKB-KW
  • histone serine kinase activity Source: GO_Central
  • protein kinase activity Source: PomBase
  • protein serine/threonine kinase activity Source: PomBase

GO - Biological processi

  • correction of merotelic kinetochore attachment, mitotic Source: PomBase
  • histone H3-S10 phosphorylation involved in chromosome condensation Source: PomBase
  • late endosome to vacuole transport Source: PomBase
  • mitotic cytokinesis Source: PomBase
  • mitotic sister chromatid biorientation Source: PomBase
  • mitotic sister chromatid segregation Source: PomBase
  • mitotic spindle assembly checkpoint Source: PomBase
  • mitotic spindle organization Source: GO_Central
  • protein localization to kinetochore Source: PomBase
  • protein localization to nucleolar rDNA repeats Source: PomBase
  • re-entry into mitotic cell cycle Source: PomBase
  • regulation of cytokinesis Source: GO_Central
Complete GO annotation...

Keywords - Molecular functioni

Kinase, Serine/threonine-protein kinase, Transferase

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BRENDAi2.7.11.1. 5613.

Names & Taxonomyi

Protein namesi
Recommended name:
Serine/threonine-protein kinase ark1 (EC:2.7.11.1)
Alternative name(s):
Aurora-related kinase 1
Gene namesi
Name:ark1
Synonyms:sex1
ORF Names:SPCC320.13c, SPCC330.16
OrganismiSchizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Taxonomic identifieri284812 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaTaphrinomycotinaSchizosaccharomycetesSchizosaccharomycetalesSchizosaccharomycetaceaeSchizosaccharomyces
Proteomesi
  • UP000002485 Componenti: Chromosome III

Organism-specific databases

EuPathDBiFungiDB:SPCC320.13c.
PomBaseiSPCC320.13c. ark1.

Subcellular locationi

GO - Cellular componenti

  • chromosome passenger complex Source: PomBase
  • condensed nuclear chromosome kinetochore Source: PomBase
  • cytoplasm Source: UniProtKB-KW
  • mitotic spindle midzone Source: PomBase
  • spindle microtubule Source: GO_Central
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Cytoskeleton, Nucleus

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi118 – 1181K → R: No phosphorylation of bir1; inhibits cytokinesis in a dominant negative manner. 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 355355Serine/threonine-protein kinase ark1PRO_0000085634Add
BLAST

Proteomic databases

MaxQBiO59790.

PTM databases

SwissPalmiO59790.

Interactioni

Subunit structurei

Interacts with bir1 and mad3 to form part of the mad2 complex involved in checkpoint activation. Interacts with pic1.3 Publications

Protein-protein interaction databases

BioGridi280112. 42 interactions.
DIPiDIP-44220N.
IntActiO59790. 2 interactions.
MINTiMINT-4782749.

Structurei

3D structure databases

ProteinModelPortaliO59790.
SMRiO59790. Positions 82-340.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini89 – 340252Protein kinasePROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. Aurora subfamily.PROSITE-ProRule annotation
Contains 1 protein kinase domain.PROSITE-ProRule annotation

Phylogenomic databases

HOGENOMiHOG000233016.
InParanoidiO59790.
KOiK08850.
OMAiVMEHPWI.
OrthoDBiEOG092C0QJU.
PhylomeDBiO59790.

Family and domain databases

InterProiIPR030616. Aur.
IPR011009. Kinase-like_dom.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR008271. Ser/Thr_kinase_AS.
[Graphical view]
PANTHERiPTHR24350. PTHR24350. 1 hit.
PfamiPF00069. Pkinase. 1 hit.
[Graphical view]
SMARTiSM00220. S_TKc. 1 hit.
[Graphical view]
SUPFAMiSSF56112. SSF56112. 1 hit.
PROSITEiPS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

O59790-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSDSKLADSL NCLSVSTPST TANPGRQQLL RLAVSNQRQV NNVSLANGKE
60 70 80 90 100
NKRTSNSKFN SSLRKIEEPI AGVPSSAGPQ WREFHIGMFE IGKPLGKGKF
110 120 130 140 150
GRVYLAKEKK TGFIVALKTL HKSELVQSKI EKQVRREIEI QSNLRHKNIL
160 170 180 190 200
RLYGHFHDEK RIYLILEFAG RGELYQHLRR AKRFSEEVAS KYIFQMANAL
210 220 230 240 250
SYLHKKHVIH RDIKPENILL GIDGEIKLSD FGWSVHAPSN RRTTLCGTLD
260 270 280 290 300
YLPPEMVEGK EHTEKVDLWS LGVLTYEFLV GAPPFEDMSG HSATYKRIAK
310 320 330 340 350
VDLKIPSFVP PDARDLISRL LQHNPEKRMS LEQVMRHPWI VKYKDSWTRK

SSESS
Length:355
Mass (Da):40,597
Last modified:April 13, 2004 - v2
Checksum:iBF15AC7B7571A301
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CU329672 Genomic DNA. Translation: CAD88263.1.
PIRiT41298.
RefSeqiNP_001018849.1. NM_001022711.2.

Genome annotation databases

EnsemblFungiiSPCC320.13c.1; SPCC320.13c.1:pep; SPCC320.13c.
GeneIDi3361036.
KEGGispo:SPCC320.13c.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CU329672 Genomic DNA. Translation: CAD88263.1.
PIRiT41298.
RefSeqiNP_001018849.1. NM_001022711.2.

3D structure databases

ProteinModelPortaliO59790.
SMRiO59790. Positions 82-340.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi280112. 42 interactions.
DIPiDIP-44220N.
IntActiO59790. 2 interactions.
MINTiMINT-4782749.

PTM databases

SwissPalmiO59790.

Proteomic databases

MaxQBiO59790.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiSPCC320.13c.1; SPCC320.13c.1:pep; SPCC320.13c.
GeneIDi3361036.
KEGGispo:SPCC320.13c.

Organism-specific databases

EuPathDBiFungiDB:SPCC320.13c.
PomBaseiSPCC320.13c. ark1.

Phylogenomic databases

HOGENOMiHOG000233016.
InParanoidiO59790.
KOiK08850.
OMAiVMEHPWI.
OrthoDBiEOG092C0QJU.
PhylomeDBiO59790.

Enzyme and pathway databases

BRENDAi2.7.11.1. 5613.

Miscellaneous databases

PROiO59790.

Family and domain databases

InterProiIPR030616. Aur.
IPR011009. Kinase-like_dom.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR008271. Ser/Thr_kinase_AS.
[Graphical view]
PANTHERiPTHR24350. PTHR24350. 1 hit.
PfamiPF00069. Pkinase. 1 hit.
[Graphical view]
SMARTiSM00220. S_TKc. 1 hit.
[Graphical view]
SUPFAMiSSF56112. SSF56112. 1 hit.
PROSITEiPS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiARK1_SCHPO
AccessioniPrimary (citable) accession number: O59790
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 20, 2002
Last sequence update: April 13, 2004
Last modified: September 7, 2016
This is version 136 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Schizosaccharomyces pombe
    Schizosaccharomyces pombe: entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.