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O59778

- BIOB_SCHPO

UniProt

O59778 - BIOB_SCHPO

Protein

Biotin synthase

Gene

bio2

Organism
Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 114 (01 Oct 2014)
      Sequence version 1 (01 Aug 1998)
      Previous versions | rss
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    Functioni

    Catalytic activityi

    Dethiobiotin + sulfur-(sulfur carrier) + 2 S-adenosyl-L-methionine = biotin + (sulfur carrier) + 2 L-methionine + 2 5'-deoxyadenosine.

    Cofactori

    Binds 1 4Fe-4S cluster. The cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine By similarity.By similarity
    Binds 1 2Fe-2S cluster. The cluster is coordinated with 3 cysteines and 1 arginine By similarity.By similarity

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi69 – 691Iron-sulfur 1 (4Fe-4S-S-AdoMet)By similarity
    Metal bindingi73 – 731Iron-sulfur 1 (4Fe-4S-S-AdoMet)By similarity
    Metal bindingi76 – 761Iron-sulfur 1 (4Fe-4S-S-AdoMet)By similarity
    Metal bindingi113 – 1131Iron-sulfur 2 (2Fe-2S)By similarity
    Metal bindingi146 – 1461Iron-sulfur 2 (2Fe-2S)By similarity
    Metal bindingi206 – 2061Iron-sulfur 2 (2Fe-2S)By similarity
    Metal bindingi280 – 2801Iron-sulfur 2 (2Fe-2S)By similarity

    GO - Molecular functioni

    1. 2 iron, 2 sulfur cluster binding Source: UniProtKB-KW
    2. 4 iron, 4 sulfur cluster binding Source: UniProtKB-KW
    3. biotin synthase activity Source: PomBase
    4. metal ion binding Source: UniProtKB-KW

    GO - Biological processi

    1. biotin biosynthetic process Source: UniProtKB-UniPathway

    Keywords - Molecular functioni

    Transferase

    Keywords - Biological processi

    Biotin biosynthesis

    Keywords - Ligandi

    2Fe-2S, 4Fe-4S, Iron, Iron-sulfur, Metal-binding, S-adenosyl-L-methionine

    Enzyme and pathway databases

    UniPathwayiUPA00078; UER00162.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Biotin synthase (EC:2.8.1.6)
    Gene namesi
    Name:bio2
    ORF Names:SPCC1235.02, SPCC320.01c
    OrganismiSchizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
    Taxonomic identifieri284812 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaTaphrinomycotinaSchizosaccharomycetesSchizosaccharomycetalesSchizosaccharomycetaceaeSchizosaccharomyces
    ProteomesiUP000002485: Chromosome III

    Organism-specific databases

    PomBaseiSPCC1235.02.

    Subcellular locationi

    GO - Cellular componenti

    1. mitochondrion Source: PomBase

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 363363Biotin synthasePRO_0000185566Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei13 – 131Phosphoserine1 Publication
    Modified residuei14 – 141Phosphoserine1 Publication
    Modified residuei17 – 171Phosphoserine1 Publication

    Keywords - PTMi

    Phosphoprotein

    Proteomic databases

    MaxQBiO59778.
    PaxDbiO59778.

    Interactioni

    Protein-protein interaction databases

    BioGridi275301. 35 interactions.
    MINTiMINT-4676108.
    STRINGi4896.SPCC1235.02-1.

    Structurei

    3D structure databases

    ProteinModelPortaliO59778.
    SMRiO59778. Positions 20-332.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Phylogenomic databases

    eggNOGiCOG0502.
    HOGENOMiHOG000239957.
    KOiK01012.
    OMAiCKEDCIF.
    OrthoDBiEOG7V76GZ.
    PhylomeDBiO59778.

    Family and domain databases

    Gene3Di3.20.20.70. 1 hit.
    HAMAPiMF_01694. BioB.
    InterProiIPR013785. Aldolase_TIM.
    IPR010722. BATS_dom.
    IPR002684. Biotin_synth/BioAB.
    IPR024177. Biotin_synthase.
    IPR006638. Elp3/MiaB/NifB.
    IPR007197. rSAM.
    [Graphical view]
    PfamiPF06968. BATS. 1 hit.
    PF04055. Radical_SAM. 1 hit.
    [Graphical view]
    PIRSFiPIRSF001619. Biotin_synth. 1 hit.
    SMARTiSM00876. BATS. 1 hit.
    SM00729. Elp3. 1 hit.
    [Graphical view]
    TIGRFAMsiTIGR00433. bioB. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    O59778-1 [UniParc]FASTAAdd to Basket

    « Hide

    MFTRTIRQQI RRSSALSLVR NNWTREEIQK IYDTPLIDLI FRAASIHRKF    50
    HDPKKVQQCT LLSIKTGGCT EDCKYCAQSS RYNTGVKATK LMKIDEVLEK 100
    AKIAKAKGST RFCMGSAWRD LNGRNRTFKN ILEIIKEVRS MDMEVCVTLG 150
    MLNEQQAKEL KDAGLTAYNH NLDTSREYYS KIISTRTYDE RLNTIDNLRK 200
    AGLKVCSGGI LGLGEKKHDR VGLIHSLATM PTHPESVPFN LLVPIPGTPV 250
    GDAVKERLPI HPFLRSIATA RICMPKTIIR FAAGRNTCSE SEQALAFMAG 300
    ANAVFTGEKM LTTPAVSWDS DSQLFYNWGL EGMQSFEYGT STEGEDGTFT 350
    LPPKERLAPS PSL 363
    Length:363
    Mass (Da):40,651
    Last modified:August 1, 1998 - v1
    Checksum:i008E2EDF9901AEB1
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti13 – 142SS → FF in CAA12229. (PubMed:10525840)Curated
    Sequence conflicti17 – 171S → F in CAA12229. (PubMed:10525840)Curated
    Sequence conflicti312 – 3187TTPAVSW → LLLLFL in CAA12229. (PubMed:10525840)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AJ224930 mRNA. Translation: CAA12229.1.
    CU329672 Genomic DNA. Translation: CAA18303.1.
    PIRiT40876.
    RefSeqiNP_587728.1. NM_001022723.2.

    Genome annotation databases

    EnsemblFungiiSPCC1235.02.1; SPCC1235.02.1:pep; SPCC1235.02.
    GeneIDi2538717.
    KEGGispo:SPCC1235.02.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AJ224930 mRNA. Translation: CAA12229.1 .
    CU329672 Genomic DNA. Translation: CAA18303.1 .
    PIRi T40876.
    RefSeqi NP_587728.1. NM_001022723.2.

    3D structure databases

    ProteinModelPortali O59778.
    SMRi O59778. Positions 20-332.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 275301. 35 interactions.
    MINTi MINT-4676108.
    STRINGi 4896.SPCC1235.02-1.

    Proteomic databases

    MaxQBi O59778.
    PaxDbi O59778.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblFungii SPCC1235.02.1 ; SPCC1235.02.1:pep ; SPCC1235.02 .
    GeneIDi 2538717.
    KEGGi spo:SPCC1235.02.

    Organism-specific databases

    PomBasei SPCC1235.02.

    Phylogenomic databases

    eggNOGi COG0502.
    HOGENOMi HOG000239957.
    KOi K01012.
    OMAi CKEDCIF.
    OrthoDBi EOG7V76GZ.
    PhylomeDBi O59778.

    Enzyme and pathway databases

    UniPathwayi UPA00078 ; UER00162 .

    Miscellaneous databases

    NextBioi 20799902.
    PROi O59778.

    Family and domain databases

    Gene3Di 3.20.20.70. 1 hit.
    HAMAPi MF_01694. BioB.
    InterProi IPR013785. Aldolase_TIM.
    IPR010722. BATS_dom.
    IPR002684. Biotin_synth/BioAB.
    IPR024177. Biotin_synthase.
    IPR006638. Elp3/MiaB/NifB.
    IPR007197. rSAM.
    [Graphical view ]
    Pfami PF06968. BATS. 1 hit.
    PF04055. Radical_SAM. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF001619. Biotin_synth. 1 hit.
    SMARTi SM00876. BATS. 1 hit.
    SM00729. Elp3. 1 hit.
    [Graphical view ]
    TIGRFAMsi TIGR00433. bioB. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "Cloning of Schizosaccharomyces pombe bio2 by heterologous complementation of a Saccharomyces cerevisiae mutant."
      Phalip V., Jeltsch J.-M., Lemoine Y.
      Curr. Microbiol. 39:348-350(1999) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA], CHARACTERIZATION.
      Strain: D18.
    2. "The genome sequence of Schizosaccharomyces pombe."
      Wood V., Gwilliam R., Rajandream M.A., Lyne M.H., Lyne R., Stewart A., Sgouros J.G., Peat N., Hayles J., Baker S.G., Basham D., Bowman S., Brooks K., Brown D., Brown S., Chillingworth T., Churcher C.M., Collins M.
      , Connor R., Cronin A., Davis P., Feltwell T., Fraser A., Gentles S., Goble A., Hamlin N., Harris D.E., Hidalgo J., Hodgson G., Holroyd S., Hornsby T., Howarth S., Huckle E.J., Hunt S., Jagels K., James K.D., Jones L., Jones M., Leather S., McDonald S., McLean J., Mooney P., Moule S., Mungall K.L., Murphy L.D., Niblett D., Odell C., Oliver K., O'Neil S., Pearson D., Quail M.A., Rabbinowitsch E., Rutherford K.M., Rutter S., Saunders D., Seeger K., Sharp S., Skelton J., Simmonds M.N., Squares R., Squares S., Stevens K., Taylor K., Taylor R.G., Tivey A., Walsh S.V., Warren T., Whitehead S., Woodward J.R., Volckaert G., Aert R., Robben J., Grymonprez B., Weltjens I., Vanstreels E., Rieger M., Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Duesterhoeft A., Fritzc C., Holzer E., Moestl D., Hilbert H., Borzym K., Langer I., Beck A., Lehrach H., Reinhardt R., Pohl T.M., Eger P., Zimmermann W., Wedler H., Wambutt R., Purnelle B., Goffeau A., Cadieu E., Dreano S., Gloux S., Lelaure V., Mottier S., Galibert F., Aves S.J., Xiang Z., Hunt C., Moore K., Hurst S.M., Lucas M., Rochet M., Gaillardin C., Tallada V.A., Garzon A., Thode G., Daga R.R., Cruzado L., Jimenez J., Sanchez M., del Rey F., Benito J., Dominguez A., Revuelta J.L., Moreno S., Armstrong J., Forsburg S.L., Cerutti L., Lowe T., McCombie W.R., Paulsen I., Potashkin J., Shpakovski G.V., Ussery D., Barrell B.G., Nurse P.
      Nature 415:871-880(2002) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: 972 / ATCC 24843.
    3. Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-13; SER-14 AND SER-17, IDENTIFICATION BY MASS SPECTROMETRY.

    Entry informationi

    Entry nameiBIOB_SCHPO
    AccessioniPrimary (citable) accession number: O59778
    Secondary accession number(s): O60050
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: January 11, 2001
    Last sequence update: August 1, 1998
    Last modified: October 1, 2014
    This is version 114 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. Schizosaccharomyces pombe
      Schizosaccharomyces pombe: entries and gene names
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3