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Protein

Mitotic spindle checkpoint component mad3

Gene

mad3

Organism
Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Has a role in transducing the anaphase inhibitory signal to the anaphase promoting complex (APC). Forms part of the mad2 feedback control.1 Publication

GO - Molecular functioni

  • anaphase-promoting complex binding Source: PomBase
  • protein kinase activity Source: GO_Central
  • ubiquitin ligase inhibitor activity Source: PomBase

GO - Biological processi

  • cell division Source: UniProtKB-KW
  • meiotic sister chromatid cohesion, centromeric Source: GO_Central
  • mitotic spindle assembly checkpoint Source: PomBase
  • negative regulation of mitotic metaphase/anaphase transition Source: PomBase
  • signal transduction involved in mitotic spindle assembly checkpoint Source: PomBase

Keywordsi

Biological processCell cycle, Cell division, Mitosis

Names & Taxonomyi

Protein namesi
Recommended name:
Mitotic spindle checkpoint component mad3
Gene namesi
Name:mad3
ORF Names:SPCC1795.01c, SPCC895.02
OrganismiSchizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Taxonomic identifieri284812 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaTaphrinomycotinaSchizosaccharomycetesSchizosaccharomycetalesSchizosaccharomycetaceaeSchizosaccharomyces
Proteomesi
  • UP000002485 Componenti: Chromosome III

Organism-specific databases

EuPathDBiFungiDB:SPCC1795.01c
PomBaseiSPCC1795.01c mad3

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Centromere, Chromosome, Kinetochore, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000845481 – 310Mitotic spindle checkpoint component mad3Add BLAST310

Proteomic databases

MaxQBiO59767
PaxDbiO59767
PRIDEiO59767

PTM databases

iPTMnetiO59767

Interactioni

Subunit structurei

Interacts with ark1, cut17, mad2 and slp1 to form part of the mad2 complex involved in checkpoint activation. Interacts also with bub3.2 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
mad2O144175EBI-1269284,EBI-1269310

Protein-protein interaction databases

BioGridi275789, 54 interactors
DIPiDIP-39397N
IntActiO59767, 4 interactors
STRINGi4896.SPCC1795.01c.1

Structurei

Secondary structure

1310
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi13 – 18Combined sources6
Helixi19 – 22Combined sources4
Helixi32 – 39Combined sources8
Helixi45 – 64Combined sources20
Helixi65 – 67Combined sources3
Helixi72 – 85Combined sources14
Turni92 – 94Combined sources3
Helixi96 – 106Combined sources11
Turni107 – 109Combined sources3
Helixi111 – 113Combined sources3
Helixi117 – 127Combined sources11
Helixi133 – 142Combined sources10
Helixi150 – 162Combined sources13
Helixi166 – 179Combined sources14
Helixi184 – 201Combined sources18
Helixi203 – 205Combined sources3

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
4AEZX-ray2.30C/F/I1-223[»]
SMRiO59767
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini56 – 218BUB1 N-terminalPROSITE-ProRule annotationAdd BLAST163

Phylogenomic databases

InParanoidiO59767
KOiK06680
OMAiERMGHER
OrthoDBiEOG092C0DAL
PhylomeDBiO59767

Family and domain databases

InterProiView protein in InterPro
IPR015661 Bub1/Mad3
IPR013212 Mad3/Bub1_I
IPR011990 TPR-like_helical_dom_sf
PANTHERiPTHR14030 PTHR14030, 1 hit
PfamiView protein in Pfam
PF08311 Mad3_BUB1_I, 1 hit
SMARTiView protein in SMART
SM00777 Mad3_BUB1_I, 1 hit
SUPFAMiSSF48452 SSF48452, 1 hit
PROSITEiView protein in PROSITE
PS51489 BUB1_N, 1 hit

Sequencei

Sequence statusi: Complete.

O59767-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MEPLDAGKNW VHMDVIEQSK ENIEPRKAGH SASALAKSSS RNHTEKEVAG
60 70 80 90 100
LQKERMGHER KIETSESLDD PLQVWIDYIK WTLDNFPQGE TKTSGLVTLL
110 120 130 140 150
ERCTREFVRN PLYKDDVRYL RIWMQYVNYI DEPVELFSFL AHHHIGQESS
160 170 180 190 200
IFYEEYANYF ESRGLFQKAD EVYQKGKRMK AKPFLRFQQK YQQFTHRWLE
210 220 230 240 250
FAPQSFSSNT NSVNPLQTTF ESTNIQEISQ SRTKISKPKF KFSVYSDADG
260 270 280 290 300
SGKDGQPGTW QTLGTVDQRR KENNISATSW VGEKLPLKSP RKLDPLGKFQ
310
VHCDEEVSKE
Length:310
Mass (Da):36,086
Last modified:August 1, 1999 - v2
Checksum:iEEC3EA6D3790C4BF
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CU329672 Genomic DNA Translation: CAA18636.1
PIRiT41640
RefSeqiNP_588043.2, NM_001023035.2

Genome annotation databases

EnsemblFungiiSPCC1795.01c.1; SPCC1795.01c.1:pep; SPCC1795.01c
GeneIDi2539219
KEGGispo:SPCC1795.01c

Similar proteinsi

Entry informationi

Entry nameiMAD3_SCHPO
AccessioniPrimary (citable) accession number: O59767
Entry historyiIntegrated into UniProtKB/Swiss-Prot: December 6, 2002
Last sequence update: August 1, 1999
Last modified: May 23, 2018
This is version 120 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

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