Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Mitotic spindle checkpoint component mad3

Gene

mad3

Organism
Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Has a role in transducing the anaphase inhibitory signal to the anaphase promoting complex (APC). Forms part of the mad2 feedback control.1 Publication

GO - Molecular functioni

  • anaphase-promoting complex binding Source: PomBase
  • ubiquitin ligase inhibitor activity Source: PomBase

GO - Biological processi

  • cell division Source: UniProtKB-KW
  • mitotic nuclear division Source: UniProtKB-KW
  • mitotic spindle assembly checkpoint Source: PomBase
  • negative regulation of mitotic metaphase/anaphase transition Source: PomBase
  • signal transduction involved in mitotic spindle assembly checkpoint Source: PomBase
Complete GO annotation...

Keywords - Biological processi

Cell cycle, Cell division, Mitosis

Names & Taxonomyi

Protein namesi
Recommended name:
Mitotic spindle checkpoint component mad3
Gene namesi
Name:mad3
ORF Names:SPCC1795.01c, SPCC895.02
OrganismiSchizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Taxonomic identifieri284812 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaTaphrinomycotinaSchizosaccharomycetesSchizosaccharomycetalesSchizosaccharomycetaceaeSchizosaccharomyces
Proteomesi
  • UP000002485 Componenti: Chromosome III

Organism-specific databases

EuPathDBiFungiDB:SPCC1795.01c.
PomBaseiSPCC1795.01c. mad3.

Subcellular locationi

  • Nucleus 1 Publication
  • Chromosomecentromerekinetochore 1 Publication

  • Note: Associated with the kinetochore.

GO - Cellular componenti

  • condensed nuclear chromosome kinetochore Source: PomBase
  • cytosol Source: PomBase
  • mitotic checkpoint complex Source: PomBase
  • nucleus Source: PomBase
Complete GO annotation...

Keywords - Cellular componenti

Centromere, Chromosome, Kinetochore, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 310310Mitotic spindle checkpoint component mad3PRO_0000084548Add
BLAST

Proteomic databases

MaxQBiO59767.

Interactioni

Subunit structurei

Interacts with ark1, cut17, mad2 and slp1 to form part of the mad2 complex involved in checkpoint activation. Interacts also with bub3.2 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
mad2O144172EBI-1269284,EBI-1269310

GO - Molecular functioni

  • anaphase-promoting complex binding Source: PomBase

Protein-protein interaction databases

BioGridi275789. 52 interactions.
DIPiDIP-39397N.
IntActiO59767. 4 interactions.
MINTiMINT-4676019.

Structurei

Secondary structure

1
310
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi13 – 186Combined sources
Helixi19 – 224Combined sources
Helixi32 – 398Combined sources
Helixi45 – 6420Combined sources
Helixi65 – 673Combined sources
Helixi72 – 8514Combined sources
Turni92 – 943Combined sources
Helixi96 – 10611Combined sources
Turni107 – 1093Combined sources
Helixi111 – 1133Combined sources
Helixi117 – 12711Combined sources
Helixi133 – 14210Combined sources
Helixi150 – 16213Combined sources
Helixi166 – 17914Combined sources
Helixi184 – 20118Combined sources
Helixi203 – 2053Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
4AEZX-ray2.30C/F/I1-223[»]
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini56 – 218163BUB1 N-terminalPROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Contains 1 BUB1 N-terminal domain.PROSITE-ProRule annotation

Phylogenomic databases

InParanoidiO59767.
KOiK06680.
OMAiERCTREL.
OrthoDBiEOG092C0DAL.
PhylomeDBiO59767.

Family and domain databases

InterProiIPR015661. Bub1/Mad3.
IPR013212. Mad3/Bub1_I.
IPR011990. TPR-like_helical_dom.
[Graphical view]
PANTHERiPTHR14030. PTHR14030. 1 hit.
PfamiPF08311. Mad3_BUB1_I. 1 hit.
[Graphical view]
SMARTiSM00777. Mad3_BUB1_I. 1 hit.
[Graphical view]
SUPFAMiSSF48452. SSF48452. 1 hit.
PROSITEiPS51489. BUB1_N. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

O59767-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MEPLDAGKNW VHMDVIEQSK ENIEPRKAGH SASALAKSSS RNHTEKEVAG
60 70 80 90 100
LQKERMGHER KIETSESLDD PLQVWIDYIK WTLDNFPQGE TKTSGLVTLL
110 120 130 140 150
ERCTREFVRN PLYKDDVRYL RIWMQYVNYI DEPVELFSFL AHHHIGQESS
160 170 180 190 200
IFYEEYANYF ESRGLFQKAD EVYQKGKRMK AKPFLRFQQK YQQFTHRWLE
210 220 230 240 250
FAPQSFSSNT NSVNPLQTTF ESTNIQEISQ SRTKISKPKF KFSVYSDADG
260 270 280 290 300
SGKDGQPGTW QTLGTVDQRR KENNISATSW VGEKLPLKSP RKLDPLGKFQ
310
VHCDEEVSKE
Length:310
Mass (Da):36,086
Last modified:August 1, 1999 - v2
Checksum:iEEC3EA6D3790C4BF
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CU329672 Genomic DNA. Translation: CAA18636.1.
PIRiT41640.
RefSeqiNP_588043.2. NM_001023035.2.

Genome annotation databases

EnsemblFungiiSPCC1795.01c.1; SPCC1795.01c.1:pep; SPCC1795.01c.
GeneIDi2539219.
KEGGispo:SPCC1795.01c.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CU329672 Genomic DNA. Translation: CAA18636.1.
PIRiT41640.
RefSeqiNP_588043.2. NM_001023035.2.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
4AEZX-ray2.30C/F/I1-223[»]
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi275789. 52 interactions.
DIPiDIP-39397N.
IntActiO59767. 4 interactions.
MINTiMINT-4676019.

Proteomic databases

MaxQBiO59767.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiSPCC1795.01c.1; SPCC1795.01c.1:pep; SPCC1795.01c.
GeneIDi2539219.
KEGGispo:SPCC1795.01c.

Organism-specific databases

EuPathDBiFungiDB:SPCC1795.01c.
PomBaseiSPCC1795.01c. mad3.

Phylogenomic databases

InParanoidiO59767.
KOiK06680.
OMAiERCTREL.
OrthoDBiEOG092C0DAL.
PhylomeDBiO59767.

Miscellaneous databases

PROiO59767.

Family and domain databases

InterProiIPR015661. Bub1/Mad3.
IPR013212. Mad3/Bub1_I.
IPR011990. TPR-like_helical_dom.
[Graphical view]
PANTHERiPTHR14030. PTHR14030. 1 hit.
PfamiPF08311. Mad3_BUB1_I. 1 hit.
[Graphical view]
SMARTiSM00777. Mad3_BUB1_I. 1 hit.
[Graphical view]
SUPFAMiSSF48452. SSF48452. 1 hit.
PROSITEiPS51489. BUB1_N. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiMAD3_SCHPO
AccessioniPrimary (citable) accession number: O59767
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 6, 2002
Last sequence update: August 1, 1999
Last modified: September 7, 2016
This is version 106 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. Schizosaccharomyces pombe
    Schizosaccharomyces pombe: entries and gene names
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.