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Protein

S-adenosyl-L-methionine-dependent tRNA 4-demethylwyosine synthase

Gene

tyw1

Organism
Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Probable component of the wybutosine biosynthesis pathway. Wybutosine is a hyper modified guanosine with a tricyclic base found at the 3'-position adjacent to the anticodon of eukaryotic phenylalanine tRNA. Catalyzes the condensation of N-methylguanine with 2 carbon atoms from pyruvate to form the tricyclic 4-demethylwyosine, an intermediate in wybutosine biosynthesis (By similarity).By similarity

Catalytic activityi

N(1)-methylguanine(37) in tRNA(Phe) + pyruvate + S-adenosyl-L-methionine = 4-demethylwyosine(37) in tRNA(Phe) + L-methionine + 5'-deoxyadenosine + CO2 + H2O.

Cofactori

[4Fe-4S] clusterBy similarityNote: Binds 1 [4Fe-4S] cluster. The cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine.By similarity

Pathwayi: wybutosine-tRNA(Phe) biosynthesis

This protein is involved in the pathway wybutosine-tRNA(Phe) biosynthesis, which is part of tRNA modification.
View all proteins of this organism that are known to be involved in the pathway wybutosine-tRNA(Phe) biosynthesis and in tRNA modification.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi357 – 3571Iron-sulfur (4Fe-4S-S-AdoMet)Sequence analysis
Metal bindingi361 – 3611Iron-sulfur (4Fe-4S-S-AdoMet)Sequence analysis
Metal bindingi364 – 3641Iron-sulfur (4Fe-4S-S-AdoMet)Sequence analysis

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi97 – 1015FMNPROSITE-ProRule annotation
Nucleotide bindingi187 – 22135FMNPROSITE-ProRule annotationAdd
BLAST

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Lyase

Keywords - Biological processi

tRNA processing

Keywords - Ligandi

4Fe-4S, Iron, Iron-sulfur, Metal-binding, Nucleotide-binding, S-adenosyl-L-methionine

Enzyme and pathway databases

UniPathwayiUPA00375.

Names & Taxonomyi

Protein namesi
Recommended name:
S-adenosyl-L-methionine-dependent tRNA 4-demethylwyosine synthase (EC:4.1.3.44)
Alternative name(s):
tRNA wybutosine-synthesizing protein 1
Gene namesi
Name:tyw1
ORF Names:SPCC1020.08
OrganismiSchizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Taxonomic identifieri284812 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaTaphrinomycotinaSchizosaccharomycetesSchizosaccharomycetalesSchizosaccharomycetaceaeSchizosaccharomyces
Proteomesi
  • UP000002485 Componenti: Chromosome III

Organism-specific databases

EuPathDBiFungiDB:SPCC1020.08.
PomBaseiSPCC1020.08. tyw1.

Subcellular locationi

GO - Cellular componenti

  • endoplasmic reticulum Source: PomBase
Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 688688S-adenosyl-L-methionine-dependent tRNA 4-demethylwyosine synthasePRO_0000281834Add
BLAST

Proteomic databases

MaxQBiO59761.

Interactioni

Protein-protein interaction databases

BioGridi275743. 6 interactions.
MINTiMINT-4675959.

Structurei

3D structure databases

ProteinModelPortaliO59761.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini91 – 247157Flavodoxin-likePROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Belongs to the TYW1 family.Curated
Contains 1 flavodoxin-like domain.PROSITE-ProRule annotation

Phylogenomic databases

HOGENOMiHOG000216584.
InParanoidiO59761.
KOiK15449.
OMAiGTTWRWV.
OrthoDBiEOG783N48.
PhylomeDBiO59761.

Family and domain databases

Gene3Di3.20.20.70. 1 hit.
3.40.50.360. 1 hit.
InterProiIPR013785. Aldolase_TIM.
IPR001094. Flavdoxin-like.
IPR008254. Flavodoxin/NO_synth.
IPR029039. Flavoprotein-like_dom.
IPR007197. rSAM.
IPR013917. tRNA_wybutosine-synth.
[Graphical view]
PfamiPF00258. Flavodoxin_1. 1 hit.
PF04055. Radical_SAM. 1 hit.
PF08608. Wyosine_form. 1 hit.
[Graphical view]
PRINTSiPR00369. FLAVODOXIN.
SUPFAMiSSF52218. SSF52218. 1 hit.
PROSITEiPS50902. FLAVODOXIN_LIKE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

O59761-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MGYSLGYIGE HWEEYRAVLY IVLLLPVLIH FLIRRKTQLS NSDKSSEKKD
60 70 80 90 100
EVKKQREVKR FKRVGKRGKI GSPSSSIRKQ NDTIDWKNSP LCVFYSTLGG
110 120 130 140 150
TAERYAKQVH EELSSLLQRD DIQLLNLDYV DLSEYFVSCP ENAIYLVVLP
160 170 180 190 200
SYEIESSIDY YLSSLQESFS DFRVPKDPLH GLSGYAVFGL GDMENYPGDK
210 220 230 240 250
FCYQAIQADK WIKKLGARRL APLGVVNTQL APTAQIDALL QWTRSVAECL
260 270 280 290 300
KNGTLLKIGN TDSLSSDVMD VEDMGSMMAK AKAEAALPVG TKEMVSTESP
310 320 330 340 350
TYKALTKQGY SVVGSHSGVK ICRWTKSAMR GRGFCYKYSF YGIRSHLCME
360 370 380 390 400
ATPSLACANK CTFCWRHGTN PVGTSWRWKV DPPEMILQGI LKAHYAKLKL
410 420 430 440 450
MKGVPGVLPD RYEEASRVRH CALSLVGEPI FYPYINEFVS MLHEREISSF
460 470 480 490 500
LVTNAQHPEA LRNMGMVTQL YVSVDASTKQ SLKSVDRPLF KDFWERMLTC
510 520 530 540 550
LEILREKRQR TVYRMTLVKG FNMEQIKEYT ELIRLGVPCF IEVKGVTYSG
560 570 580 590 600
NSDQSPLTMK NVPYYEEVID FVKKLIEYID IHLQDLGVRY EIAAEHAHSC
610 620 630 640 650
SILVAQTAFK KDGHWHTHID YPKFFELIRT KKDFGPFDYM ASTPDFAMFG
660 670 680
NGGFSPEDTR FHRKKKTQTS KPISATISET ATISEAAA
Length:688
Mass (Da):78,188
Last modified:August 1, 1998 - v1
Checksum:i9A3449DBF528FB40
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CU329672 Genomic DNA. Translation: CAA18996.1.
PIRiT40832.
RefSeqiNP_587951.1. NM_001022942.2.

Genome annotation databases

EnsemblFungiiSPCC1020.08.1; SPCC1020.08.1:pep; SPCC1020.08.
GeneIDi2539172.
KEGGispo:SPCC1020.08.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CU329672 Genomic DNA. Translation: CAA18996.1.
PIRiT40832.
RefSeqiNP_587951.1. NM_001022942.2.

3D structure databases

ProteinModelPortaliO59761.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi275743. 6 interactions.
MINTiMINT-4675959.

Proteomic databases

MaxQBiO59761.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiSPCC1020.08.1; SPCC1020.08.1:pep; SPCC1020.08.
GeneIDi2539172.
KEGGispo:SPCC1020.08.

Organism-specific databases

EuPathDBiFungiDB:SPCC1020.08.
PomBaseiSPCC1020.08. tyw1.

Phylogenomic databases

HOGENOMiHOG000216584.
InParanoidiO59761.
KOiK15449.
OMAiGTTWRWV.
OrthoDBiEOG783N48.
PhylomeDBiO59761.

Enzyme and pathway databases

UniPathwayiUPA00375.

Miscellaneous databases

PROiO59761.

Family and domain databases

Gene3Di3.20.20.70. 1 hit.
3.40.50.360. 1 hit.
InterProiIPR013785. Aldolase_TIM.
IPR001094. Flavdoxin-like.
IPR008254. Flavodoxin/NO_synth.
IPR029039. Flavoprotein-like_dom.
IPR007197. rSAM.
IPR013917. tRNA_wybutosine-synth.
[Graphical view]
PfamiPF00258. Flavodoxin_1. 1 hit.
PF04055. Radical_SAM. 1 hit.
PF08608. Wyosine_form. 1 hit.
[Graphical view]
PRINTSiPR00369. FLAVODOXIN.
SUPFAMiSSF52218. SSF52218. 1 hit.
PROSITEiPS50902. FLAVODOXIN_LIKE. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "The genome sequence of Schizosaccharomyces pombe."
    Wood V., Gwilliam R., Rajandream M.A., Lyne M.H., Lyne R., Stewart A., Sgouros J.G., Peat N., Hayles J., Baker S.G., Basham D., Bowman S., Brooks K., Brown D., Brown S., Chillingworth T., Churcher C.M., Collins M.
    , Connor R., Cronin A., Davis P., Feltwell T., Fraser A., Gentles S., Goble A., Hamlin N., Harris D.E., Hidalgo J., Hodgson G., Holroyd S., Hornsby T., Howarth S., Huckle E.J., Hunt S., Jagels K., James K.D., Jones L., Jones M., Leather S., McDonald S., McLean J., Mooney P., Moule S., Mungall K.L., Murphy L.D., Niblett D., Odell C., Oliver K., O'Neil S., Pearson D., Quail M.A., Rabbinowitsch E., Rutherford K.M., Rutter S., Saunders D., Seeger K., Sharp S., Skelton J., Simmonds M.N., Squares R., Squares S., Stevens K., Taylor K., Taylor R.G., Tivey A., Walsh S.V., Warren T., Whitehead S., Woodward J.R., Volckaert G., Aert R., Robben J., Grymonprez B., Weltjens I., Vanstreels E., Rieger M., Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Duesterhoeft A., Fritzc C., Holzer E., Moestl D., Hilbert H., Borzym K., Langer I., Beck A., Lehrach H., Reinhardt R., Pohl T.M., Eger P., Zimmermann W., Wedler H., Wambutt R., Purnelle B., Goffeau A., Cadieu E., Dreano S., Gloux S., Lelaure V., Mottier S., Galibert F., Aves S.J., Xiang Z., Hunt C., Moore K., Hurst S.M., Lucas M., Rochet M., Gaillardin C., Tallada V.A., Garzon A., Thode G., Daga R.R., Cruzado L., Jimenez J., Sanchez M., del Rey F., Benito J., Dominguez A., Revuelta J.L., Moreno S., Armstrong J., Forsburg S.L., Cerutti L., Lowe T., McCombie W.R., Paulsen I., Potashkin J., Shpakovski G.V., Ussery D., Barrell B.G., Nurse P.
    Nature 415:871-880(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: 972 / ATCC 24843.

Entry informationi

Entry nameiTYW1_SCHPO
AccessioniPrimary (citable) accession number: O59761
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 3, 2007
Last sequence update: August 1, 1998
Last modified: July 6, 2016
This is version 94 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. Schizosaccharomyces pombe
    Schizosaccharomyces pombe: entries and gene names
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.