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Protein

2-isopropylmalate synthase

Gene

leu3

Organism
Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the condensation of the acetyl group of acetyl-CoA with 3-methyl-2-oxobutanoate (2-oxoisovalerate) to form 3-carboxy-3-hydroxy-4-methylpentanoate (2-isopropylmalate).

Catalytic activityi

Acetyl-CoA + 3-methyl-2-oxobutanoate + H2O = (2S)-2-isopropylmalate + CoA.

Pathwayi: L-leucine biosynthesis

This protein is involved in step 1 of the subpathway that synthesizes L-leucine from 3-methyl-2-oxobutanoate.
Proteins known to be involved in the 4 steps of the subpathway in this organism are:
  1. 2-isopropylmalate synthase (leu3)
  2. 3-isopropylmalate dehydratase (leu2)
  3. 3-isopropylmalate dehydrogenase (leu1)
  4. no protein annotated in this organism
This subpathway is part of the pathway L-leucine biosynthesis, which is itself part of Amino-acid biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes L-leucine from 3-methyl-2-oxobutanoate, the pathway L-leucine biosynthesis and in Amino-acid biosynthesis.

GO - Molecular functioni

GO - Biological processi

  • branched-chain amino acid biosynthetic process Source: UniProtKB-KW
  • leucine biosynthetic process Source: PomBase
Complete GO annotation...

Keywords - Molecular functioni

Transferase

Keywords - Biological processi

Amino-acid biosynthesis, Branched-chain amino acid biosynthesis, Leucine biosynthesis

Enzyme and pathway databases

UniPathwayiUPA00048; UER00070.

Names & Taxonomyi

Protein namesi
Recommended name:
2-isopropylmalate synthase (EC:2.3.3.13)
Alternative name(s):
Alpha-IPM synthase
Alpha-isopropylmalate synthase
Gene namesi
Name:leu3
ORF Names:SPBC3E7.16c, SPBC4F6.03c
OrganismiSchizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Taxonomic identifieri284812 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaTaphrinomycotinaSchizosaccharomycetesSchizosaccharomycetalesSchizosaccharomycetaceaeSchizosaccharomyces
Proteomesi
  • UP000002485 Componenti: Chromosome II

Organism-specific databases

EuPathDBiFungiDB:SPBC3E7.16c.
PomBaseiSPBC3E7.16c. leu3.

Subcellular locationi

GO - Cellular componenti

  • mitochondrion Source: PomBase
Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001404451 – 5842-isopropylmalate synthaseAdd BLAST584

Proteomic databases

MaxQBiO59736.
PRIDEiO59736.

PTM databases

SwissPalmiO59736.

Interactioni

Protein-protein interaction databases

BioGridi277518. 6 interactors.
MINTiMINT-4675773.

Structurei

3D structure databases

ProteinModelPortaliO59736.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini45 – 323Pyruvate carboxyltransferasePROSITE-ProRule annotationAdd BLAST279

Sequence similaritiesi

Contains 1 pyruvate carboxyltransferase domain.PROSITE-ProRule annotation

Phylogenomic databases

HOGENOMiHOG000110941.
InParanoidiO59736.
KOiK01649.
OMAiVERCNQI.
OrthoDBiEOG092C18QN.
PhylomeDBiO59736.

Family and domain databases

Gene3Di3.20.20.70. 1 hit.
HAMAPiMF_00572. LeuA_type2. 1 hit.
InterProiIPR013709. 2-isopropylmalate_synth_dimer.
IPR002034. AIPM/Hcit_synth_CS.
IPR013785. Aldolase_TIM.
IPR005668. IPM_Synthase.
IPR000891. PYR_CT.
[Graphical view]
PfamiPF00682. HMGL-like. 1 hit.
PF08502. LeuA_dimer. 1 hit.
[Graphical view]
SMARTiSM00917. LeuA_dimer. 1 hit.
[Graphical view]
SUPFAMiSSF110921. SSF110921. 1 hit.
TIGRFAMsiTIGR00970. leuA_yeast. 1 hit.
PROSITEiPS00815. AIPM_HOMOCIT_SYNTH_1. 1 hit.
PS00816. AIPM_HOMOCIT_SYNTH_2. 1 hit.
PS50991. PYR_CT. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

O59736-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKSTFEAAGR VAKGMLKDPS KKYKPFKGIQ LPNRQWPNKV LTKAPRWLST
60 70 80 90 100
DLRDGNQALP DPMNGQEKLR YFKLLCSIGF KEIEVGFPSA SQTDFAFVRH
110 120 130 140 150
LIETPGLIPD DVTISALTPS REPLILRTIE ALRGAKNATV HLYNACSPLF
160 170 180 190 200
REVVFRNSKQ ETLDLAIKGS KIVTAATKNA LESKETNWGF EYSPETFSDT
210 220 230 240 250
EPDFALEVCE AVKGMWKPSA AQPIIFNLPA TVEMSTPNTY ADLIEYFSTN
260 270 280 290 300
ISEREKVCVS LHPHNDRGTA VAAAELGQLA GGDRIEGCLF GNGERTGNVD
310 320 330 340 350
LVTLAFNLYT QGVSPNLDFS KLDEIIRITE DCNKINVHPR HPYAGNLVFT
360 370 380 390 400
AFSGSHQDAI SKGLKAYDER KAVDPVWKVP YLPLDPHDVN SEYAAIIRVN
410 420 430 440 450
SQSGKGGVAY LLKTNCGLDL PRALQVEFGS IVKDYSDTKG KELSIGEISD
460 470 480 490 500
LFYTTYYLEF PGRFSVNDYT LSSNGPQSKC IKCVVDIKGE KKDTPSRVVI
510 520 530 540 550
EGVGNGPLSA LVDALRRQFN ISFDIGQYSE HAIGSGNGVK AASYVEIIFN
560 570 580
NTSFWGVGID ADVTSAGLKA VMSGVSRASR AFAK
Length:584
Mass (Da):64,042
Last modified:May 1, 1999 - v2
Checksum:i9371A03F82C032F6
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CU329671 Genomic DNA. Translation: CAA19019.1.
PIRiT40500.
RefSeqiNP_596103.2. NM_001022019.3.

Genome annotation databases

EnsemblFungiiSPBC3E7.16c.1; SPBC3E7.16c.1:pep; SPBC3E7.16c.
GeneIDi2541003.
KEGGispo:SPBC3E7.16c.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CU329671 Genomic DNA. Translation: CAA19019.1.
PIRiT40500.
RefSeqiNP_596103.2. NM_001022019.3.

3D structure databases

ProteinModelPortaliO59736.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi277518. 6 interactors.
MINTiMINT-4675773.

PTM databases

SwissPalmiO59736.

Proteomic databases

MaxQBiO59736.
PRIDEiO59736.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiSPBC3E7.16c.1; SPBC3E7.16c.1:pep; SPBC3E7.16c.
GeneIDi2541003.
KEGGispo:SPBC3E7.16c.

Organism-specific databases

EuPathDBiFungiDB:SPBC3E7.16c.
PomBaseiSPBC3E7.16c. leu3.

Phylogenomic databases

HOGENOMiHOG000110941.
InParanoidiO59736.
KOiK01649.
OMAiVERCNQI.
OrthoDBiEOG092C18QN.
PhylomeDBiO59736.

Enzyme and pathway databases

UniPathwayiUPA00048; UER00070.

Miscellaneous databases

PROiO59736.

Family and domain databases

Gene3Di3.20.20.70. 1 hit.
HAMAPiMF_00572. LeuA_type2. 1 hit.
InterProiIPR013709. 2-isopropylmalate_synth_dimer.
IPR002034. AIPM/Hcit_synth_CS.
IPR013785. Aldolase_TIM.
IPR005668. IPM_Synthase.
IPR000891. PYR_CT.
[Graphical view]
PfamiPF00682. HMGL-like. 1 hit.
PF08502. LeuA_dimer. 1 hit.
[Graphical view]
SMARTiSM00917. LeuA_dimer. 1 hit.
[Graphical view]
SUPFAMiSSF110921. SSF110921. 1 hit.
TIGRFAMsiTIGR00970. leuA_yeast. 1 hit.
PROSITEiPS00815. AIPM_HOMOCIT_SYNTH_1. 1 hit.
PS00816. AIPM_HOMOCIT_SYNTH_2. 1 hit.
PS50991. PYR_CT. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiLEU1_SCHPO
AccessioniPrimary (citable) accession number: O59736
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 30, 2000
Last sequence update: May 1, 1999
Last modified: November 2, 2016
This is version 115 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. Schizosaccharomyces pombe
    Schizosaccharomyces pombe: entries and gene names
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.