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O59735 (LAC1_SCHPO) Reviewed, UniProtKB/Swiss-Prot

Last modified January 25, 2012. Version 80. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
Sphingosine N-acyltransferase lac1
EC=2.3.1.24
Alternative name(s):
Meiotically up-regulated gene 83 protein
Gene names
Name:lac1
Synonyms:mug83
ORF Names:SPBC3E7.15c, SPBC4F6.02c
OrganismSchizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Taxonomic identifier284812 [NCBI]
Taxonomic lineageEukaryota › Fungi › Dikarya › Ascomycota › Taphrinomycotina › Schizosaccharomycetes › Schizosaccharomycetales › Schizosaccharomycetaceae › Schizosaccharomyces

Protein attributes

Sequence length384 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Component of the ceramide synthase complex required for C26-CoA-dependent ceramide synthesis. Facilitates ER-to-Golgi transport of GPI-anchored proteins By similarity. Has a role in meiosis. Ref.2

Catalytic activity

Acyl-CoA + sphingosine = CoA + N-acylsphingosine.

Subcellular location

Endoplasmic reticulum membrane; Multi-pass membrane protein By similarity.

Sequence similarities

Belongs to the sphingosine N-acyltransferase family.

Contains 1 TLC (TRAM/LAG1/CLN8) domain.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 384384Sphingosine N-acyltransferase lac1
PRO_0000185534

Regions

Topological domain1 – 5858Cytoplasmic By similarity
Transmembrane59 – 7921Helical; Potential
Topological domain80 – 10324Lumenal By similarity
Transmembrane104 – 12421Helical; Potential
Topological domain125 – 15430Cytoplasmic By similarity
Transmembrane155 – 17521Helical; Potential
Topological domain176 – 19419Lumenal By similarity
Transmembrane195 – 21521Helical; Potential
Topological domain216 – 23217Cytoplasmic By similarity
Transmembrane233 – 25321Helical; Potential
Topological domain254 – 26916Lumenal By similarity
Transmembrane270 – 29021Helical; Potential
Topological domain291 – 32838Cytoplasmic By similarity
Transmembrane329 – 34921Helical; Potential
Topological domain350 – 38435Lumenal By similarity
Domain141 – 357217TLC

Amino acid modifications

Modified residue411Phosphoserine Ref.3
Modified residue3611Phosphothreonine Ref.3
Modified residue3661Phosphoserine Ref.3
Glycosylation3591N-linked (GlcNAc...) Potential

Sequences

Sequence LengthMass (Da)Tools
O59735 [UniParc].

Last modified May 1, 2000. Version 2.
Checksum: 82FCF8EA6638849A

FASTA38445,336
        10         20         30         40         50         60 
MGNNTSRRSQ SQKFKNIPSI SAGSFSTMPV QHRGRRRRSK SIVGRAAQNA VLRSKEKTWI 

        70         80         90        100        110        120 
VPLILLTLLV GWYFVNPNGY IKYGIFLSYP IPGTNPAQYG KGRLDIAFCL FYALFFTFCR 

       130        140        150        160        170        180 
EFIMQEIIAR IGRHFNIRAP AKLRRFEEQA YTCLYFTVMG SWGLYVMKQT PMWFFNTDAF 

       190        200        210        220        230        240 
WEEYPHFYHV GSFKAFYLIE AAYWIQQALV LILQLEKPRK DFKELVVHHI ITLLLIGLSY 

       250        260        270        280        290        300 
YFHFTWIGLA VFITMDTSDI WLALSKCLNY VNTVIVYPIF VIFVFVWIYM RHYLNFKIMW 

       310        320        330        340        350        360 
AVWGTMRTIN SFDLDWAAEQ YKCWISRDVT LILLTALQLV NIYWLILILR IGYRAFTTND 

       370        380 
THDERSEDED EEVSDEKSSA KKND

« Hide

References

« Hide 'large scale' references
[1]"The genome sequence of Schizosaccharomyces pombe."
Wood V., Gwilliam R., Rajandream M.A., Lyne M.H., Lyne R., Stewart A., Sgouros J.G., Peat N., Hayles J., Baker S.G., Basham D., Bowman S., Brooks K., Brown D., Brown S., Chillingworth T., Churcher C.M., Collins M. expand/collapse author list , Connor R., Cronin A., Davis P., Feltwell T., Fraser A., Gentles S., Goble A., Hamlin N., Harris D.E., Hidalgo J., Hodgson G., Holroyd S., Hornsby T., Howarth S., Huckle E.J., Hunt S., Jagels K., James K.D., Jones L., Jones M., Leather S., McDonald S., McLean J., Mooney P., Moule S., Mungall K.L., Murphy L.D., Niblett D., Odell C., Oliver K., O'Neil S., Pearson D., Quail M.A., Rabbinowitsch E., Rutherford K.M., Rutter S., Saunders D., Seeger K., Sharp S., Skelton J., Simmonds M.N., Squares R., Squares S., Stevens K., Taylor K., Taylor R.G., Tivey A., Walsh S.V., Warren T., Whitehead S., Woodward J.R., Volckaert G., Aert R., Robben J., Grymonprez B., Weltjens I., Vanstreels E., Rieger M., Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Duesterhoeft A., Fritzc C., Holzer E., Moestl D., Hilbert H., Borzym K., Langer I., Beck A., Lehrach H., Reinhardt R., Pohl T.M., Eger P., Zimmermann W., Wedler H., Wambutt R., Purnelle B., Goffeau A., Cadieu E., Dreano S., Gloux S., Lelaure V., Mottier S., Galibert F., Aves S.J., Xiang Z., Hunt C., Moore K., Hurst S.M., Lucas M., Rochet M., Gaillardin C., Tallada V.A., Garzon A., Thode G., Daga R.R., Cruzado L., Jimenez J., Sanchez M., del Rey F., Benito J., Dominguez A., Revuelta J.L., Moreno S., Armstrong J., Forsburg S.L., Cerutti L., Lowe T., McCombie W.R., Paulsen I., Potashkin J., Shpakovski G.V., Ussery D., Barrell B.G., Nurse P.
Nature 415:871-880(2002) [PubMed: 11859360] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: 972 / ATCC 24843.
[2]"A large-scale screen in S. pombe identifies seven novel genes required for critical meiotic events."
Martin-Castellanos C., Blanco M., Rozalen A.E., Perez-Hidalgo L., Garcia A.I., Conde F., Mata J., Ellermeier C., Davis L., San-Segundo P., Smith G.R., Moreno S.
Curr. Biol. 15:2056-2062(2005) [PubMed: 16303567] [Abstract]
Cited for: FUNCTION IN MEIOSIS.
[3]"Phosphoproteome analysis of fission yeast."
Wilson-Grady J.T., Villen J., Gygi S.P.
J. Proteome Res. 7:1088-1097(2008) [PubMed: 18257517] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-41; THR-361 AND SER-366, MASS SPECTROMETRY.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		CU329671 Genomic DNA. Translation: CAA19018.2.
PIRT40389.
RefSeqNP_596102.1. NM_001022018.1.

3D structure databases

ModBaseSearch...

Protein-protein interaction databases

STRINGO59735.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblFungiSPBC3E7.15c.1; SPBC3E7.15c.1:pep; SPBC3E7.15c.
GeneID2540466.
GenomeReviewsGene locus lac1 in contig CU329671_GR.
KEGGspo:SPBC3E7.15c.
NMPDRfig|4896.1.peg.1968.

Organism-specific databases

GeneDB_SpombeSPBC3E7.15c.

Phylogenomic databases

eggNOGfuNOG05004.
GeneTreeEFGT00050000005520.
HOGENOMHBG738741.
OMAYKCWISQ.
OrthoDBEOG447K3B.

Enzyme and pathway databases

BioCycSPOM-XXX-01:SPOM-XXX-01-003754-MONOMER.

Gene expression databases

ArrayExpressO59735.

Family and domain databases

InterProIPR016439. Longevity_assurance_LAG1_LAC1.
IPR006634. TLC-dom.
IPR013599. TRAM1.
[Graphical view]
KOK04709.
PfamPF08390. TRAM1. 1 hit.
PF03798. TRAM_LAG1_CLN8. 1 hit.
[Graphical view]
PIRSFPIRSF005225. LAG1_LAC1. 1 hit.
SMARTSM00724. TLC. 1 hit.
[Graphical view]
PROSITEPS50922. TLC. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameLAC1_SCHPO
AccessionPrimary (citable) accession number: O59735
Entry history
Integrated into UniProtKB/Swiss-Prot: August 29, 2001
Last sequence update: May 1, 2000
Last modified: January 25, 2012
This is version 80 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Relevant documents

Schizosaccharomyces pombe

Schizosaccharomyces pombe: entries and gene names

SIMILARITY comments

Index of protein domains and families

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