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O59721

- PUS4_SCHPO

UniProt

O59721 - PUS4_SCHPO

Protein

Probable tRNA pseudouridine synthase 4

Gene

SPBC11C11.10

Organism
Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 94 (01 Oct 2014)
      Sequence version 2 (01 May 1999)
      Previous versions | rss
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    Functioni

    Responsible for synthesis of pseudouridine from uracil-55 in the psi GC loop of transfer RNAs.By similarity

    Catalytic activityi

    tRNA uridine(55) = tRNA pseudouridine(55).

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei115 – 1151NucleophileBy similarity

    GO - Molecular functioni

    1. pseudouridine synthase activity Source: PomBase
    2. RNA binding Source: InterPro

    GO - Biological processi

    1. tRNA pseudouridine synthesis Source: PomBase

    Keywords - Molecular functioni

    Isomerase

    Keywords - Biological processi

    tRNA processing

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Probable tRNA pseudouridine synthase 4 (EC:5.4.99.25)
    Alternative name(s):
    tRNA pseudouridine(55) synthase
    Short name:
    Psi55 synthase
    tRNA pseudouridylate synthase 4
    tRNA-uridine isomerase 4
    Gene namesi
    ORF Names:SPBC11C11.10, SPBC3B8.13c
    OrganismiSchizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
    Taxonomic identifieri284812 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaTaphrinomycotinaSchizosaccharomycetesSchizosaccharomycetalesSchizosaccharomycetaceaeSchizosaccharomyces
    ProteomesiUP000002485: Chromosome II

    Organism-specific databases

    PomBaseiSPBC11C11.10.

    Subcellular locationi

    Nucleus By similarity. Mitochondrion By similarity

    GO - Cellular componenti

    1. mitochondrion Source: PomBase
    2. nucleus Source: PomBase

    Keywords - Cellular componenti

    Mitochondrion, Nucleus

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 407407Probable tRNA pseudouridine synthase 4PRO_0000121975Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei291 – 2911Phosphoserine1 Publication
    Modified residuei293 – 2931Phosphothreonine1 Publication
    Modified residuei296 – 2961Phosphoserine1 Publication
    Modified residuei406 – 4061Phosphothreonine1 Publication

    Keywords - PTMi

    Phosphoprotein

    Proteomic databases

    MaxQBiO59721.

    Interactioni

    Protein-protein interaction databases

    BioGridi276567. 25 interactions.
    MINTiMINT-4675673.
    STRINGi4896.SPBC11C11.10-1.

    Structurei

    3D structure databases

    ProteinModelPortaliO59721.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the pseudouridine synthase TruB family.Curated

    Phylogenomic databases

    eggNOGiCOG0130.
    KOiK03177.
    OMAiGVFAINK.
    OrthoDBiEOG7WHHKZ.
    PhylomeDBiO59721.

    Family and domain databases

    HAMAPiMF_01080. TruB_bact.
    InterProiIPR002501. PsdUridine_synth.
    IPR020103. PsdUridine_synth_cat_dom.
    IPR014780. tRNA_psdUridine_synth_TruB.
    [Graphical view]
    PANTHERiPTHR13767. PTHR13767. 1 hit.
    PfamiPF01509. TruB_N. 1 hit.
    [Graphical view]
    SUPFAMiSSF55120. SSF55120. 3 hits.

    Sequencei

    Sequence statusi: Complete.

    O59721-1 [UniParc]FASTAAdd to Basket

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    MTVTNTYNPT LFFTRVVFQR ITKFTAKANE ENEILFYALF VADLCLDMKG    50
    GLIAINKPSG RTSAQCLNEL KKIISNSELA QYFRPAPPHP NDRNRRRRKS 100
    NRLPDIKIGH GGTLDPLASG VLVVGLGTGT KQLSSLLSCM KTYRATALFG 150
    CSTDTYDSAG KIIKIAVHIP TKEEILSGLD AFRGDISQLP PLYSALHIQG 200
    KRLYEYAREG IPLPESIKAR SMHCEELILK DFIPKEEHTY TDPDEFASKE 250
    AIESEELLRP IEGGAERHDL LAKTEQDINP QDGDEKINAK SPTTNSVTDV 300
    AKDQTVTNPK KRKFEVTDLA RGSRPAIGPI AVLDMTVSSG FYVRSLIHDL 350
    GRQVNSEAHM VDLVRLKQGS FALDDENCFD FSEFSAPGWE EKLAAAFKID 400
    LKGDETS 407
    Length:407
    Mass (Da):45,166
    Last modified:May 1, 1999 - v2
    Checksum:iD0CB88C503C774DB
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CU329671 Genomic DNA. Translation: CAA20692.1.
    PIRiT39326.
    RefSeqiNP_596400.1. NM_001022320.2.

    Genome annotation databases

    EnsemblFungiiSPBC11C11.10.1; SPBC11C11.10.1:pep; SPBC11C11.10.
    GeneIDi2540023.
    KEGGispo:SPBC11C11.10.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CU329671 Genomic DNA. Translation: CAA20692.1 .
    PIRi T39326.
    RefSeqi NP_596400.1. NM_001022320.2.

    3D structure databases

    ProteinModelPortali O59721.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 276567. 25 interactions.
    MINTi MINT-4675673.
    STRINGi 4896.SPBC11C11.10-1.

    Proteomic databases

    MaxQBi O59721.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblFungii SPBC11C11.10.1 ; SPBC11C11.10.1:pep ; SPBC11C11.10 .
    GeneIDi 2540023.
    KEGGi spo:SPBC11C11.10.

    Organism-specific databases

    PomBasei SPBC11C11.10.

    Phylogenomic databases

    eggNOGi COG0130.
    KOi K03177.
    OMAi GVFAINK.
    OrthoDBi EOG7WHHKZ.
    PhylomeDBi O59721.

    Miscellaneous databases

    NextBioi 20801166.

    Family and domain databases

    HAMAPi MF_01080. TruB_bact.
    InterProi IPR002501. PsdUridine_synth.
    IPR020103. PsdUridine_synth_cat_dom.
    IPR014780. tRNA_psdUridine_synth_TruB.
    [Graphical view ]
    PANTHERi PTHR13767. PTHR13767. 1 hit.
    Pfami PF01509. TruB_N. 1 hit.
    [Graphical view ]
    SUPFAMi SSF55120. SSF55120. 3 hits.
    ProtoNeti Search...

    Publicationsi

    1. "The genome sequence of Schizosaccharomyces pombe."
      Wood V., Gwilliam R., Rajandream M.A., Lyne M.H., Lyne R., Stewart A., Sgouros J.G., Peat N., Hayles J., Baker S.G., Basham D., Bowman S., Brooks K., Brown D., Brown S., Chillingworth T., Churcher C.M., Collins M.
      , Connor R., Cronin A., Davis P., Feltwell T., Fraser A., Gentles S., Goble A., Hamlin N., Harris D.E., Hidalgo J., Hodgson G., Holroyd S., Hornsby T., Howarth S., Huckle E.J., Hunt S., Jagels K., James K.D., Jones L., Jones M., Leather S., McDonald S., McLean J., Mooney P., Moule S., Mungall K.L., Murphy L.D., Niblett D., Odell C., Oliver K., O'Neil S., Pearson D., Quail M.A., Rabbinowitsch E., Rutherford K.M., Rutter S., Saunders D., Seeger K., Sharp S., Skelton J., Simmonds M.N., Squares R., Squares S., Stevens K., Taylor K., Taylor R.G., Tivey A., Walsh S.V., Warren T., Whitehead S., Woodward J.R., Volckaert G., Aert R., Robben J., Grymonprez B., Weltjens I., Vanstreels E., Rieger M., Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Duesterhoeft A., Fritzc C., Holzer E., Moestl D., Hilbert H., Borzym K., Langer I., Beck A., Lehrach H., Reinhardt R., Pohl T.M., Eger P., Zimmermann W., Wedler H., Wambutt R., Purnelle B., Goffeau A., Cadieu E., Dreano S., Gloux S., Lelaure V., Mottier S., Galibert F., Aves S.J., Xiang Z., Hunt C., Moore K., Hurst S.M., Lucas M., Rochet M., Gaillardin C., Tallada V.A., Garzon A., Thode G., Daga R.R., Cruzado L., Jimenez J., Sanchez M., del Rey F., Benito J., Dominguez A., Revuelta J.L., Moreno S., Armstrong J., Forsburg S.L., Cerutti L., Lowe T., McCombie W.R., Paulsen I., Potashkin J., Shpakovski G.V., Ussery D., Barrell B.G., Nurse P.
      Nature 415:871-880(2002) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: 972 / ATCC 24843.
    2. Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-291; THR-293; SER-296 AND THR-406, IDENTIFICATION BY MASS SPECTROMETRY.

    Entry informationi

    Entry nameiPUS4_SCHPO
    AccessioniPrimary (citable) accession number: O59721
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: November 1, 2002
    Last sequence update: May 1, 1999
    Last modified: October 1, 2014
    This is version 94 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Schizosaccharomyces pombe
      Schizosaccharomyces pombe: entries and gene names
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3