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O59721 (PUS4_SCHPO) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 92. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Probable tRNA pseudouridine synthase 4

EC=5.4.99.25
Alternative name(s):
tRNA pseudouridine(55) synthase
Short name=Psi55 synthase
tRNA pseudouridylate synthase 4
tRNA-uridine isomerase 4
Gene names
ORF Names:SPBC11C11.10, SPBC3B8.13c
OrganismSchizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast) [Reference proteome]
Taxonomic identifier284812 [NCBI]
Taxonomic lineageEukaryotaFungiDikaryaAscomycotaTaphrinomycotinaSchizosaccharomycetesSchizosaccharomycetalesSchizosaccharomycetaceaeSchizosaccharomyces

Protein attributes

Sequence length407 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Responsible for synthesis of pseudouridine from uracil-55 in the psi GC loop of transfer RNAs By similarity. HAMAP-Rule MF_01080

Catalytic activity

tRNA uridine55 = tRNA pseudouridine55. HAMAP-Rule MF_01080

Subcellular location

Nucleus By similarity. Mitochondrion By similarity HAMAP-Rule MF_01080.

Sequence similarities

Belongs to the pseudouridine synthase TruB family.

Ontologies

Keywords
   Biological processtRNA processing
   Cellular componentMitochondrion
Nucleus
   Molecular functionIsomerase
   PTMPhosphoprotein
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processtRNA pseudouridine synthesis

Inferred by curator. Source: PomBase

   Cellular_componentmitochondrion

Inferred from sequence or structural similarity. Source: PomBase

nucleus

Inferred from direct assay PubMed 16823372. Source: PomBase

   Molecular_functionRNA binding

Inferred from electronic annotation. Source: InterPro

pseudouridine synthase activity

Inferred from sequence orthology. Source: PomBase

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 407407Probable tRNA pseudouridine synthase 4 HAMAP-Rule MF_01080
PRO_0000121975

Sites

Active site1151Nucleophile By similarity

Amino acid modifications

Modified residue2911Phosphoserine Ref.2
Modified residue2931Phosphothreonine Ref.2
Modified residue2961Phosphoserine Ref.2
Modified residue4061Phosphothreonine Ref.2

Sequences

Sequence LengthMass (Da)Tools
O59721 [UniParc].

Last modified May 1, 1999. Version 2.
Checksum: D0CB88C503C774DB

FASTA40745,166
        10         20         30         40         50         60 
MTVTNTYNPT LFFTRVVFQR ITKFTAKANE ENEILFYALF VADLCLDMKG GLIAINKPSG 

        70         80         90        100        110        120 
RTSAQCLNEL KKIISNSELA QYFRPAPPHP NDRNRRRRKS NRLPDIKIGH GGTLDPLASG 

       130        140        150        160        170        180 
VLVVGLGTGT KQLSSLLSCM KTYRATALFG CSTDTYDSAG KIIKIAVHIP TKEEILSGLD 

       190        200        210        220        230        240 
AFRGDISQLP PLYSALHIQG KRLYEYAREG IPLPESIKAR SMHCEELILK DFIPKEEHTY 

       250        260        270        280        290        300 
TDPDEFASKE AIESEELLRP IEGGAERHDL LAKTEQDINP QDGDEKINAK SPTTNSVTDV 

       310        320        330        340        350        360 
AKDQTVTNPK KRKFEVTDLA RGSRPAIGPI AVLDMTVSSG FYVRSLIHDL GRQVNSEAHM 

       370        380        390        400 
VDLVRLKQGS FALDDENCFD FSEFSAPGWE EKLAAAFKID LKGDETS 

« Hide

References

[1]"The genome sequence of Schizosaccharomyces pombe."
Wood V., Gwilliam R., Rajandream M.A., Lyne M.H., Lyne R., Stewart A., Sgouros J.G., Peat N., Hayles J., Baker S.G., Basham D., Bowman S., Brooks K., Brown D., Brown S., Chillingworth T., Churcher C.M., Collins M. expand/collapse author list , Connor R., Cronin A., Davis P., Feltwell T., Fraser A., Gentles S., Goble A., Hamlin N., Harris D.E., Hidalgo J., Hodgson G., Holroyd S., Hornsby T., Howarth S., Huckle E.J., Hunt S., Jagels K., James K.D., Jones L., Jones M., Leather S., McDonald S., McLean J., Mooney P., Moule S., Mungall K.L., Murphy L.D., Niblett D., Odell C., Oliver K., O'Neil S., Pearson D., Quail M.A., Rabbinowitsch E., Rutherford K.M., Rutter S., Saunders D., Seeger K., Sharp S., Skelton J., Simmonds M.N., Squares R., Squares S., Stevens K., Taylor K., Taylor R.G., Tivey A., Walsh S.V., Warren T., Whitehead S., Woodward J.R., Volckaert G., Aert R., Robben J., Grymonprez B., Weltjens I., Vanstreels E., Rieger M., Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Duesterhoeft A., Fritzc C., Holzer E., Moestl D., Hilbert H., Borzym K., Langer I., Beck A., Lehrach H., Reinhardt R., Pohl T.M., Eger P., Zimmermann W., Wedler H., Wambutt R., Purnelle B., Goffeau A., Cadieu E., Dreano S., Gloux S., Lelaure V., Mottier S., Galibert F., Aves S.J., Xiang Z., Hunt C., Moore K., Hurst S.M., Lucas M., Rochet M., Gaillardin C., Tallada V.A., Garzon A., Thode G., Daga R.R., Cruzado L., Jimenez J., Sanchez M., del Rey F., Benito J., Dominguez A., Revuelta J.L., Moreno S., Armstrong J., Forsburg S.L., Cerutti L., Lowe T., McCombie W.R., Paulsen I., Potashkin J., Shpakovski G.V., Ussery D., Barrell B.G., Nurse P.
Nature 415:871-880(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: 972 / ATCC 24843.
[2]"Phosphoproteome analysis of fission yeast."
Wilson-Grady J.T., Villen J., Gygi S.P.
J. Proteome Res. 7:1088-1097(2008) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-291; THR-293; SER-296 AND THR-406, IDENTIFICATION BY MASS SPECTROMETRY.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CU329671 Genomic DNA. Translation: CAA20692.1.
PIRT39326.
RefSeqNP_596400.1. NM_001022320.2.

3D structure databases

ProteinModelPortalO59721.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid276567. 25 interactions.
MINTMINT-4675673.
STRING4896.SPBC11C11.10-1.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblFungiSPBC11C11.10.1; SPBC11C11.10.1:pep; SPBC11C11.10.
GeneID2540023.
KEGGspo:SPBC11C11.10.

Organism-specific databases

PomBaseSPBC11C11.10.

Phylogenomic databases

eggNOGCOG0130.
KOK03177.
OMAGVFAINK.
OrthoDBEOG7WHHKZ.
PhylomeDBO59721.

Family and domain databases

HAMAPMF_01080. TruB_bact.
InterProIPR002501. PsdUridine_synth.
IPR020103. PsdUridine_synth_cat_dom.
IPR014780. tRNA_psdUridine_synth_TruB.
[Graphical view]
PANTHERPTHR13767. PTHR13767. 1 hit.
PfamPF01509. TruB_N. 1 hit.
[Graphical view]
SUPFAMSSF55120. SSF55120. 3 hits.
ProtoNetSearch...

Other

NextBio20801166.

Entry information

Entry namePUS4_SCHPO
AccessionPrimary (citable) accession number: O59721
Entry history
Integrated into UniProtKB/Swiss-Prot: November 1, 2002
Last sequence update: May 1, 1999
Last modified: April 16, 2014
This is version 92 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Schizosaccharomyces pombe

Schizosaccharomyces pombe: entries and gene names