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Protein

Probable tRNA pseudouridine synthase 4

Gene

SPBC11C11.10

Organism
Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Responsible for synthesis of pseudouridine from uracil-55 in the psi GC loop of transfer RNAs.By similarity

Catalytic activityi

tRNA uridine(55) = tRNA pseudouridine(55).

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei115 – 1151NucleophileBy similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Isomerase

Keywords - Biological processi

tRNA processing

Names & Taxonomyi

Protein namesi
Recommended name:
Probable tRNA pseudouridine synthase 4 (EC:5.4.99.25)
Alternative name(s):
tRNA pseudouridine(55) synthase
Short name:
Psi55 synthase
tRNA pseudouridylate synthase 4
tRNA-uridine isomerase 4
Gene namesi
ORF Names:SPBC11C11.10, SPBC3B8.13c
OrganismiSchizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Taxonomic identifieri284812 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaTaphrinomycotinaSchizosaccharomycetesSchizosaccharomycetalesSchizosaccharomycetaceaeSchizosaccharomyces
ProteomesiUP000002485 Componenti: Chromosome II

Organism-specific databases

EuPathDBiFungiDB:SPBC11C11.10.
PomBaseiSPBC11C11.10.

Subcellular locationi

GO - Cellular componenti

  • mitochondrion Source: PomBase
  • nucleus Source: PomBase
Complete GO annotation...

Keywords - Cellular componenti

Mitochondrion, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 407407Probable tRNA pseudouridine synthase 4PRO_0000121975Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei291 – 2911Phosphoserine1 Publication
Modified residuei293 – 2931Phosphothreonine1 Publication
Modified residuei296 – 2961Phosphoserine1 Publication
Modified residuei406 – 4061Phosphothreonine1 Publication

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiO59721.

Interactioni

Protein-protein interaction databases

BioGridi276567. 26 interactions.
MINTiMINT-4675673.

Structurei

3D structure databases

ProteinModelPortaliO59721.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the pseudouridine synthase TruB family.Curated

Phylogenomic databases

eggNOGiCOG0130.
InParanoidiO59721.
KOiK03177.
OMAiGVFAINK.
OrthoDBiEOG7WHHKZ.
PhylomeDBiO59721.

Family and domain databases

HAMAPiMF_01080. TruB_bact.
InterProiIPR002501. PsdUridine_synth.
IPR020103. PsdUridine_synth_cat_dom.
IPR014780. tRNA_psdUridine_synth_TruB.
[Graphical view]
PANTHERiPTHR13767. PTHR13767. 1 hit.
PfamiPF01509. TruB_N. 1 hit.
[Graphical view]
SUPFAMiSSF55120. SSF55120. 3 hits.

Sequencei

Sequence statusi: Complete.

O59721-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTVTNTYNPT LFFTRVVFQR ITKFTAKANE ENEILFYALF VADLCLDMKG
60 70 80 90 100
GLIAINKPSG RTSAQCLNEL KKIISNSELA QYFRPAPPHP NDRNRRRRKS
110 120 130 140 150
NRLPDIKIGH GGTLDPLASG VLVVGLGTGT KQLSSLLSCM KTYRATALFG
160 170 180 190 200
CSTDTYDSAG KIIKIAVHIP TKEEILSGLD AFRGDISQLP PLYSALHIQG
210 220 230 240 250
KRLYEYAREG IPLPESIKAR SMHCEELILK DFIPKEEHTY TDPDEFASKE
260 270 280 290 300
AIESEELLRP IEGGAERHDL LAKTEQDINP QDGDEKINAK SPTTNSVTDV
310 320 330 340 350
AKDQTVTNPK KRKFEVTDLA RGSRPAIGPI AVLDMTVSSG FYVRSLIHDL
360 370 380 390 400
GRQVNSEAHM VDLVRLKQGS FALDDENCFD FSEFSAPGWE EKLAAAFKID

LKGDETS
Length:407
Mass (Da):45,166
Last modified:May 1, 1999 - v2
Checksum:iD0CB88C503C774DB
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CU329671 Genomic DNA. Translation: CAA20692.1.
PIRiT39326.
RefSeqiNP_596400.1. NM_001022320.2.

Genome annotation databases

EnsemblFungiiSPBC11C11.10.1; SPBC11C11.10.1:pep; SPBC11C11.10.
GeneIDi2540023.
KEGGispo:SPBC11C11.10.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CU329671 Genomic DNA. Translation: CAA20692.1.
PIRiT39326.
RefSeqiNP_596400.1. NM_001022320.2.

3D structure databases

ProteinModelPortaliO59721.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi276567. 26 interactions.
MINTiMINT-4675673.

Proteomic databases

MaxQBiO59721.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiSPBC11C11.10.1; SPBC11C11.10.1:pep; SPBC11C11.10.
GeneIDi2540023.
KEGGispo:SPBC11C11.10.

Organism-specific databases

EuPathDBiFungiDB:SPBC11C11.10.
PomBaseiSPBC11C11.10.

Phylogenomic databases

eggNOGiCOG0130.
InParanoidiO59721.
KOiK03177.
OMAiGVFAINK.
OrthoDBiEOG7WHHKZ.
PhylomeDBiO59721.

Miscellaneous databases

NextBioi20801166.
PROiO59721.

Family and domain databases

HAMAPiMF_01080. TruB_bact.
InterProiIPR002501. PsdUridine_synth.
IPR020103. PsdUridine_synth_cat_dom.
IPR014780. tRNA_psdUridine_synth_TruB.
[Graphical view]
PANTHERiPTHR13767. PTHR13767. 1 hit.
PfamiPF01509. TruB_N. 1 hit.
[Graphical view]
SUPFAMiSSF55120. SSF55120. 3 hits.
ProtoNetiSearch...

Publicationsi

  1. "The genome sequence of Schizosaccharomyces pombe."
    Wood V., Gwilliam R., Rajandream M.A., Lyne M.H., Lyne R., Stewart A., Sgouros J.G., Peat N., Hayles J., Baker S.G., Basham D., Bowman S., Brooks K., Brown D., Brown S., Chillingworth T., Churcher C.M., Collins M.
    , Connor R., Cronin A., Davis P., Feltwell T., Fraser A., Gentles S., Goble A., Hamlin N., Harris D.E., Hidalgo J., Hodgson G., Holroyd S., Hornsby T., Howarth S., Huckle E.J., Hunt S., Jagels K., James K.D., Jones L., Jones M., Leather S., McDonald S., McLean J., Mooney P., Moule S., Mungall K.L., Murphy L.D., Niblett D., Odell C., Oliver K., O'Neil S., Pearson D., Quail M.A., Rabbinowitsch E., Rutherford K.M., Rutter S., Saunders D., Seeger K., Sharp S., Skelton J., Simmonds M.N., Squares R., Squares S., Stevens K., Taylor K., Taylor R.G., Tivey A., Walsh S.V., Warren T., Whitehead S., Woodward J.R., Volckaert G., Aert R., Robben J., Grymonprez B., Weltjens I., Vanstreels E., Rieger M., Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Duesterhoeft A., Fritzc C., Holzer E., Moestl D., Hilbert H., Borzym K., Langer I., Beck A., Lehrach H., Reinhardt R., Pohl T.M., Eger P., Zimmermann W., Wedler H., Wambutt R., Purnelle B., Goffeau A., Cadieu E., Dreano S., Gloux S., Lelaure V., Mottier S., Galibert F., Aves S.J., Xiang Z., Hunt C., Moore K., Hurst S.M., Lucas M., Rochet M., Gaillardin C., Tallada V.A., Garzon A., Thode G., Daga R.R., Cruzado L., Jimenez J., Sanchez M., del Rey F., Benito J., Dominguez A., Revuelta J.L., Moreno S., Armstrong J., Forsburg S.L., Cerutti L., Lowe T., McCombie W.R., Paulsen I., Potashkin J., Shpakovski G.V., Ussery D., Barrell B.G., Nurse P.
    Nature 415:871-880(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: 972 / ATCC 24843.
  2. Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-291; THR-293; SER-296 AND THR-406, IDENTIFICATION BY MASS SPECTROMETRY.

Entry informationi

Entry nameiPUS4_SCHPO
AccessioniPrimary (citable) accession number: O59721
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 2002
Last sequence update: May 1, 1999
Last modified: July 22, 2015
This is version 101 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Schizosaccharomyces pombe
    Schizosaccharomyces pombe: entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.