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Protein

Nuclear envelope morphology protein 1

Gene

nem1

Organism
Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Protein inferred from homologyi

Functioni

Catalytic component of the nem1-spo7 complex which acts as a phosphatase and may be required for proper nuclear membrane morphology.By similarity

Catalytic activityi

[a protein]-serine/threonine phosphate + H2O = [a protein]-serine/threonine + phosphate.

GO - Molecular functioni

  1. phosphoprotein phosphatase activity Source: UniProtKB-KW

GO - Biological processi

  1. ascospore formation Source: PomBase
  2. nuclear envelope organization Source: PomBase
  3. protein dephosphorylation Source: PomBase
  4. regulation of lipid biosynthetic process Source: PomBase
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Protein phosphatase

Names & Taxonomyi

Protein namesi
Recommended name:
Nuclear envelope morphology protein 1 (EC:3.1.3.16)
Gene namesi
Name:nem1
ORF Names:SPBC3B8.10c
OrganismiSchizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Taxonomic identifieri284812 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaTaphrinomycotinaSchizosaccharomycetesSchizosaccharomycetalesSchizosaccharomycetaceaeSchizosaccharomyces
ProteomesiUP000002485: Chromosome II

Organism-specific databases

PomBaseiSPBC3B8.10c.

Subcellular locationi

Endoplasmic reticulum membrane 1 Publication; Single-pass membrane protein 1 Publication. Nucleus membrane By similarity; Single-pass membrane protein By similarity

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transmembranei123 – 13917HelicalSequence AnalysisAdd
BLAST

GO - Cellular componenti

  1. endoplasmic reticulum Source: PomBase
  2. endoplasmic reticulum membrane Source: UniProtKB-SubCell
  3. integral component of membrane Source: UniProtKB-KW
  4. Nem1-Spo7 phosphatase complex Source: PomBase
  5. nuclear membrane Source: UniProtKB-SubCell
  6. nuclear outer membrane-endoplasmic reticulum membrane network Source: PomBase
Complete GO annotation...

Keywords - Cellular componenti

Endoplasmic reticulum, Membrane, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 476476Nuclear envelope morphology protein 1PRO_0000315975Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi237 – 2371N-linked (GlcNAc...)Sequence Analysis
Glycosylationi257 – 2571N-linked (GlcNAc...)Sequence Analysis
Glycosylationi284 – 2841N-linked (GlcNAc...)Sequence Analysis
Glycosylationi356 – 3561N-linked (GlcNAc...)Sequence Analysis

Keywords - PTMi

Glycoprotein

Proteomic databases

MaxQBiO59718.

Interactioni

Subunit structurei

Component of the nem1-spo7 complex.By similarity

Protein-protein interaction databases

BioGridi277536. 94 interactions.
MINTiMINT-4675647.
STRINGi4896.SPBC3B8.10c-1.

Structurei

3D structure databases

ProteinModelPortaliO59718.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini299 – 460162FCP1 homologyPROSITE-ProRule annotationAdd
BLAST

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi158 – 278121Ser-richAdd
BLAST

Sequence similaritiesi

Belongs to the Dullard family.Curated
Contains 1 FCP1 homology domain.PROSITE-ProRule annotation

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiCOG5190.
InParanoidiO59718.
KOiK17617.
OrthoDBiEOG7VDZ0W.
PhylomeDBiO59718.

Family and domain databases

Gene3Di3.40.50.1000. 1 hit.
InterProiIPR011948. Dullard_phosphatase.
IPR023214. HAD-like_dom.
IPR004274. NIF.
[Graphical view]
PfamiPF03031. NIF. 1 hit.
[Graphical view]
SMARTiSM00577. CPDc. 1 hit.
[Graphical view]
SUPFAMiSSF56784. SSF56784. 1 hit.
TIGRFAMsiTIGR02251. HIF-SF_euk. 1 hit.
PROSITEiPS50969. FCP1. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

O59718-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MNSIARLSDE INKAILATPL DDDEADKEKL ANARGRASSA TLRHYNRRRS
60 70 80 90 100
SYSASSLSSL SSKPTEKEVP TRNEKPKHAN IMRVVVYWIR VFLKRIYTFF
110 120 130 140 150
VHSARVFLYH FLNEEKEFTL ASFFWGLCRF VFFPVLLSYK RREMLPPQPS
160 170 180 190 200
VRRPRFYSSY SYPSSHQDPA YSSFKRHRSS NSYSSSSNGN HVRFQPSIAE
210 220 230 240 250
EEISFNSFSN SLNSEEDVCV SPMKPKEVSL MGKANSNRSG HSHQPQSTQF
260 270 280 290 300
SPPANDNISK LPSSFTIVND PLKSPSSSRL RIRNITLCAD KIPRPLLNSK
310 320 330 340 350
LPRKTLVLDL DETLIHSVSR GSRTTSGQPI EVHVPGEHPI LYYIHKRPHL
360 370 380 390 400
DYFLSNVSQW FRLILFTASV QPYADPIIDY LERDKKIFAK RYYRQHCALV
410 420 430 440 450
DSSFVKDISI CNIHLSRIMI IDNSPASYNA HKENAIPIEG WISDPSDVDL
460 470
LNLLSFLHAL QYVHDVRDLL GLRLAK
Length:476
Mass (Da):54,598
Last modified:August 1, 1998 - v1
Checksum:i012BBD6EF067090F
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CU329671 Genomic DNA. Translation: CAA18299.1.
PIRiT40330.
RefSeqiNP_596404.1. NM_001022323.2.

Genome annotation databases

EnsemblFungiiSPBC3B8.10c.1; SPBC3B8.10c.1:pep; SPBC3B8.10c.
GeneIDi2541021.
KEGGispo:SPBC3B8.10c.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CU329671 Genomic DNA. Translation: CAA18299.1.
PIRiT40330.
RefSeqiNP_596404.1. NM_001022323.2.

3D structure databases

ProteinModelPortaliO59718.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi277536. 94 interactions.
MINTiMINT-4675647.
STRINGi4896.SPBC3B8.10c-1.

Proteomic databases

MaxQBiO59718.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiSPBC3B8.10c.1; SPBC3B8.10c.1:pep; SPBC3B8.10c.
GeneIDi2541021.
KEGGispo:SPBC3B8.10c.

Organism-specific databases

PomBaseiSPBC3B8.10c.

Phylogenomic databases

eggNOGiCOG5190.
InParanoidiO59718.
KOiK17617.
OrthoDBiEOG7VDZ0W.
PhylomeDBiO59718.

Miscellaneous databases

NextBioi20802135.

Family and domain databases

Gene3Di3.40.50.1000. 1 hit.
InterProiIPR011948. Dullard_phosphatase.
IPR023214. HAD-like_dom.
IPR004274. NIF.
[Graphical view]
PfamiPF03031. NIF. 1 hit.
[Graphical view]
SMARTiSM00577. CPDc. 1 hit.
[Graphical view]
SUPFAMiSSF56784. SSF56784. 1 hit.
TIGRFAMsiTIGR02251. HIF-SF_euk. 1 hit.
PROSITEiPS50969. FCP1. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "The genome sequence of Schizosaccharomyces pombe."
    Wood V., Gwilliam R., Rajandream M.A., Lyne M.H., Lyne R., Stewart A., Sgouros J.G., Peat N., Hayles J., Baker S.G., Basham D., Bowman S., Brooks K., Brown D., Brown S., Chillingworth T., Churcher C.M., Collins M.
    , Connor R., Cronin A., Davis P., Feltwell T., Fraser A., Gentles S., Goble A., Hamlin N., Harris D.E., Hidalgo J., Hodgson G., Holroyd S., Hornsby T., Howarth S., Huckle E.J., Hunt S., Jagels K., James K.D., Jones L., Jones M., Leather S., McDonald S., McLean J., Mooney P., Moule S., Mungall K.L., Murphy L.D., Niblett D., Odell C., Oliver K., O'Neil S., Pearson D., Quail M.A., Rabbinowitsch E., Rutherford K.M., Rutter S., Saunders D., Seeger K., Sharp S., Skelton J., Simmonds M.N., Squares R., Squares S., Stevens K., Taylor K., Taylor R.G., Tivey A., Walsh S.V., Warren T., Whitehead S., Woodward J.R., Volckaert G., Aert R., Robben J., Grymonprez B., Weltjens I., Vanstreels E., Rieger M., Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Duesterhoeft A., Fritzc C., Holzer E., Moestl D., Hilbert H., Borzym K., Langer I., Beck A., Lehrach H., Reinhardt R., Pohl T.M., Eger P., Zimmermann W., Wedler H., Wambutt R., Purnelle B., Goffeau A., Cadieu E., Dreano S., Gloux S., Lelaure V., Mottier S., Galibert F., Aves S.J., Xiang Z., Hunt C., Moore K., Hurst S.M., Lucas M., Rochet M., Gaillardin C., Tallada V.A., Garzon A., Thode G., Daga R.R., Cruzado L., Jimenez J., Sanchez M., del Rey F., Benito J., Dominguez A., Revuelta J.L., Moreno S., Armstrong J., Forsburg S.L., Cerutti L., Lowe T., McCombie W.R., Paulsen I., Potashkin J., Shpakovski G.V., Ussery D., Barrell B.G., Nurse P.
    Nature 415:871-880(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: 972 / ATCC 24843.
  2. "ORFeome cloning and global analysis of protein localization in the fission yeast Schizosaccharomyces pombe."
    Matsuyama A., Arai R., Yashiroda Y., Shirai A., Kamata A., Sekido S., Kobayashi Y., Hashimoto A., Hamamoto M., Hiraoka Y., Horinouchi S., Yoshida M.
    Nat. Biotechnol. 24:841-847(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiNEM1_SCHPO
AccessioniPrimary (citable) accession number: O59718
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 15, 2008
Last sequence update: August 1, 1998
Last modified: January 7, 2015
This is version 79 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Schizosaccharomyces pombe
    Schizosaccharomyces pombe: entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.