O59711 (LYS9_SCHPO) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Saccharopine dehydrogenase [NADP(+), L-glutamate-forming]
EC=1.5.1.10

Alternative name(s):
Saccharopine reductase

Gene names

Name:	lys9
ORF Names:	SPBC3B8.03

Organism

Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast) [Reference proteome]

Taxonomic identifier

284812 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Fungi › Dikarya › Ascomycota › Taphrinomycotina › Schizosaccharomycetes › Schizosaccharomycetales › Schizosaccharomycetaceae › Schizosaccharomyces › Schizosaccharomyces pombe

Protein attributes

Sequence length	450 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	N(6)-(L-1,3-dicarboxypropyl)-L-lysine + NADP+ + H2O = L-glutamate + (S)-2-amino-6-oxohexanoate + NADPH. UniProtKB Q9P4R4
Pathway	Amino-acid biosynthesis; L-lysine biosynthesis via AAA pathway; L-lysine from L-alpha-aminoadipate (fungal route): step 2/3. UniProtKB Q9P4R4
Subunit structure	Homodimer By similarity. UniProtKB Q9P4R4
Subcellular location	Cytoplasm Ref.2.
Sequence similarities	Belongs to the saccharopine dehydrogenase family.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Lysine biosynthesis
Cellular component	Cytoplasm
Ligand	NADP
Molecular function	Oxidoreductase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	lysine biosynthetic process Inferred from mutant phenotype PubMed 20537132. Source: PomBase lysine biosynthetic process via aminoadipic acid Inferred from electronic annotation. Source: UniProtKB-UniPathway
Cellular_component	cytosol Inferred from direct assay Ref.2. Source: PomBase
Molecular_function	nucleotide binding Inferred from electronic annotation. Source: InterPro saccharopine dehydrogenase (NADP+, L-glutamate-forming) activity Inferred from sequence orthology. Source: PomBase
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 450	450	Saccharopine dehydrogenase [NADP(+), L-glutamate-forming]		PRO_0000316856

Sequences

	Sequence		Length	Mass (Da)
	O59711 [UniParc]. Last modified August 1, 1998. Version 1. Checksum: 1AEE17A423715C71	FASTA	450	49,938
	10 20 30 40 50 60 MPSILLLGSG FVAHPTLEYL SRRKENNITV ACRTLSKAEA FINGIPNSKA IALDVNDEAA 70 80 90 100 110 120 LEKAVSEHDL TISLIPYTYH ATVMKAAIKH GKHVCTTSYV NPKMAELEEA AIKAGSICMN 130 140 150 160 170 180 EIGVDPGIDH LYAIKTIEEV HKAGGKIKSF LSYCGGLPAP EDSNNPLGYK FSWSSRGVLL 190 200 210 220 230 240 ALRNSAKFYE NGKLVEIDGK DLMETAKPYF IYPGYAFVCY PNRDSTVYQE RYQIPEAETI 250 260 270 280 290 300 IRGTLRYQGF PEFIHCLVDM GFLDETAQEY LSPEAPALPW KEVTARVIKA ESSSEADLIK 310 320 330 340 350 360 KISSIHKFKD EDDKKRILNG LKWLGMFSSK PVTPRGNPLD TLCATLEELM QYEEGERDML 370 380 390 400 410 420 ILQHKFEVET KEGKRQTRTC TLLDYGVPNG YTSMAKLVGV PCGVATQQIL DGVINTPGVL 430 440 450 APNDMKLCGP LIDTLAKEGI RLEEEIIDEE

References

[1]

"The genome sequence of Schizosaccharomyces pombe." Wood V., Gwilliam R., Rajandream M.A., Lyne M.H., Lyne R., Stewart A., Sgouros J.G., Peat N., Hayles J., Baker S.G., Basham D., Bowman S., Brooks K., Brown D., Brown S., Chillingworth T., Churcher C.M., Collins M. , Connor R., Cronin A., Davis P., Feltwell T., Fraser A., Gentles S., Goble A., Hamlin N., Harris D.E., Hidalgo J., Hodgson G., Holroyd S., Hornsby T., Howarth S., Huckle E.J., Hunt S., Jagels K., James K.D., Jones L., Jones M., Leather S., McDonald S., McLean J., Mooney P., Moule S., Mungall K.L., Murphy L.D., Niblett D., Odell C., Oliver K., O'Neil S., Pearson D., Quail M.A., Rabbinowitsch E., Rutherford K.M., Rutter S., Saunders D., Seeger K., Sharp S., Skelton J., Simmonds M.N., Squares R., Squares S., Stevens K., Taylor K., Taylor R.G., Tivey A., Walsh S.V., Warren T., Whitehead S., Woodward J.R., Volckaert G., Aert R., Robben J., Grymonprez B., Weltjens I., Vanstreels E., Rieger M., Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Duesterhoeft A., Fritzc C., Holzer E., Moestl D., Hilbert H., Borzym K., Langer I., Beck A., Lehrach H., Reinhardt R., Pohl T.M., Eger P., Zimmermann W., Wedler H., Wambutt R., Purnelle B., Goffeau A., Cadieu E., Dreano S., Gloux S., Lelaure V., Mottier S., Galibert F., Aves S.J., Xiang Z., Hunt C., Moore K., Hurst S.M., Lucas M., Rochet M., Gaillardin C., Tallada V.A., Garzon A., Thode G., Daga R.R., Cruzado L., Jimenez J., Sanchez M., del Rey F., Benito J., Dominguez A., Revuelta J.L., Moreno S., Armstrong J., Forsburg S.L., Cerutti L., Lowe T., McCombie W.R., Paulsen I., Potashkin J., Shpakovski G.V., Ussery D., Barrell B.G., Nurse P. Nature 415:871-880(2002) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: 972 / ATCC 24843.

[2]

"ORFeome cloning and global analysis of protein localization in the fission yeast Schizosaccharomyces pombe." Matsuyama A., Arai R., Yashiroda Y., Shirai A., Kamata A., Sekido S., Kobayashi Y., Hashimoto A., Hamamoto M., Hiraoka Y., Horinouchi S., Yoshida M. Nat. Biotechnol. 24:841-847(2006) [PubMed] [Europe PMC] [Abstract] Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].

Cross-references

Sequence databases
EMBL GenBank DDBJ	CU329671 Genomic DNA. Translation: CAA18292.1.
PIR	T40337.
RefSeq	NP_596411.1. NM_001022330.2.
3D structure databases
HSSP	HSSP built from PDB template 1FF9 based on UniProtKB Q9P4R4.
ProteinModelPortal	O59711.
SMR	O59711. Positions 3-447.
ModBase	Search...
Protein-protein interaction databases
STRING	4896.SPBC3B8.03-1.
Proteomic databases
PaxDb	O59711.
PRIDE	O59711.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	SPBC3B8.03.1; SPBC3B8.03.1:pep; SPBC3B8.03.
GeneID	2540908.
KEGG	spo:SPBC3B8.03.
Organism-specific databases
PomBase	SPBC3B8.03.
Phylogenomic databases
eggNOG	COG1748.
HOGENOM	HOG000023158.
KO	K00293.
OMA	VTVACRT.
OrthoDB	EOG4STWD0.
Enzyme and pathway databases
UniPathway	UPA00033; UER00033.
Family and domain databases
Gene3D	3.40.50.720. 1 hit.
InterPro	IPR016040. NAD(P)-bd_dom. IPR005097. Saccharopine_DH/HSpermid_syn. [Graphical view]
Pfam	PF03435. Saccharop_dh. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	20802023.

Entry information

Entry name

LYS9_SCHPO

Accession

Primary (citable) accession number: O59711

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 5, 2008
Last sequence update:	August 1, 1998
Last modified:	May 1, 2013
This is version 73 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program