Reviewed, UniProtKB/Swiss-Prot O59711 (LYS9_SCHPO)

Names and origin

Protein names

Recommended name:
    Saccharopine dehydrogenase [NADP+, L-glutamate-forming]
    EC=1.5.1.10
Alternative name(s):
    Saccharopine reductase

Gene names

Name:	lys9
ORF Names:	SPBC3B8.03

Organism

Schizosaccharomyces pombe (Fission yeast) [Complete proteome]

Taxonomic identifier

4896 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Taphrinomycotina › Schizosaccharomycetes › Schizosaccharomycetales › Schizosaccharomycetaceae › Schizosaccharomyces

Protein attributes

Sequence length	450 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Catalytic activity	N(6)-(L-1,3-dicarboxypropyl)-L-lysine + NADP+ + H2O = L-glutamate + L-2-aminoadipate 6-semialdehyde + NADPH. UniProtKB Q9P4R4
Pathway	Amino-acid biosynthesis; L-lysine biosynthesis via AAA pathway; L-lysine from L-alpha-aminoadipate (fungal route): step 2/3. UniProtKB Q9P4R4
Subunit structure	Homodimer By similarity. UniProtKB Q9P4R4
Subcellular location	Cytoplasm Ref.2.
Sequence similarities	Belongs to the saccharopine dehydrogenase family.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Lysine biosynthesis
Cellular component	Cytoplasm
Ligand	NADP
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	lysine biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytosol Ref.2 Inferred from direct assay. Source: GeneDB_SPombe
Molecular function	binding Inferred from electronic annotation. Source: InterPro saccharopine dehydrogenase (NADP+, L-glutamate-forming) activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 450	450	Saccharopine dehydrogenase [NADP+, L-glutamate-forming]		PRO_0000316856

Sequences

	Sequence		Length	Mass (Da)
	O59711-1 [UniParc]. Last modified August 1, 1998. Version 1. Checksum: 1AEE17A423715C71	FASTA	450	49,938
	10 20 30 40 50 60 MPSILLLGSG FVAHPTLEYL SRRKENNITV ACRTLSKAEA FINGIPNSKA IALDVNDEAA 70 80 90 100 110 120 LEKAVSEHDL TISLIPYTYH ATVMKAAIKH GKHVCTTSYV NPKMAELEEA AIKAGSICMN 130 140 150 160 170 180 EIGVDPGIDH LYAIKTIEEV HKAGGKIKSF LSYCGGLPAP EDSNNPLGYK FSWSSRGVLL 190 200 210 220 230 240 ALRNSAKFYE NGKLVEIDGK DLMETAKPYF IYPGYAFVCY PNRDSTVYQE RYQIPEAETI 250 260 270 280 290 300 IRGTLRYQGF PEFIHCLVDM GFLDETAQEY LSPEAPALPW KEVTARVIKA ESSSEADLIK 310 320 330 340 350 360 KISSIHKFKD EDDKKRILNG LKWLGMFSSK PVTPRGNPLD TLCATLEELM QYEEGERDML 370 380 390 400 410 420 ILQHKFEVET KEGKRQTRTC TLLDYGVPNG YTSMAKLVGV PCGVATQQIL DGVINTPGVL 430 440 450 APNDMKLCGP LIDTLAKEGI RLEEEIIDEE

References

[1]

"The genome sequence of Schizosaccharomyces pombe." Wood V., Gwilliam R., Rajandream M.A., Lyne M.H., Lyne R., Stewart A., Sgouros J.G., Peat N., Hayles J., Baker S.G., Basham D., Bowman S., Brooks K., Brown D., Brown S., Chillingworth T., Churcher C.M., Collins M. , Connor R., Cronin A., Davis P., Feltwell T., Fraser A., Gentles S., Goble A., Hamlin N., Harris D.E., Hidalgo J., Hodgson G., Holroyd S., Hornsby T., Howarth S., Huckle E.J., Hunt S., Jagels K., James K.D., Jones L., Jones M., Leather S., McDonald S., McLean J., Mooney P., Moule S., Mungall K.L., Murphy L.D., Niblett D., Odell C., Oliver K., O'Neil S., Pearson D., Quail M.A., Rabbinowitsch E., Rutherford K.M., Rutter S., Saunders D., Seeger K., Sharp S., Skelton J., Simmonds M.N., Squares R., Squares S., Stevens K., Taylor K., Taylor R.G., Tivey A., Walsh S.V., Warren T., Whitehead S., Woodward J.R., Volckaert G., Aert R., Robben J., Grymonprez B., Weltjens I., Vanstreels E., Rieger M., Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Duesterhoeft A., Fritzc C., Holzer E., Moestl D., Hilbert H., Borzym K., Langer I., Beck A., Lehrach H., Reinhardt R., Pohl T.M., Eger P., Zimmermann W., Wedler H., Wambutt R., Purnelle B., Goffeau A., Cadieu E., Dreano S., Gloux S., Lelaure V., Mottier S., Galibert F., Aves S.J., Xiang Z., Hunt C., Moore K., Hurst S.M., Lucas M., Rochet M., Gaillardin C., Tallada V.A., Garzon A., Thode G., Daga R.R., Cruzado L., Jimenez J., Sanchez M., del Rey F., Benito J., Dominguez A., Revuelta J.L., Moreno S., Armstrong J., Forsburg S.L., Cerutti L., Lowe T., McCombie W.R., Paulsen I., Potashkin J., Shpakovski G.V., Ussery D., Barrell B.G., Nurse P. Nature 415:871-880(2002) [PubMed: 11859360] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 38366 / 972.

[2]

"ORFeome cloning and global analysis of protein localization in the fission yeast Schizosaccharomyces pombe." Matsuyama A., Arai R., Yashiroda Y., Shirai A., Kamata A., Sekido S., Kobayashi Y., Hashimoto A., Hamamoto M., Hiraoka Y., Horinouchi S., Yoshida M. Nat. Biotechnol. 24:841-847(2006) [PubMed: 16823372] [Abstract] Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].

Cross-references

Sequence databases
EMBL GenBank DDBJ	CU329671 Genomic DNA. Translation: CAA18292.1.
PIR	T40337.
RefSeq	NP_596411.1.
3D structure databases
HSSP	HSSP built from PDB template 1FF9 based on UniProtKB Q9P4R4.
SMR	O59711. Positions 3-447.
ModBase	Search...
Protein-protein interaction databases
STRING	O59711.
Genome annotation databases
GeneID	2540908.
GenomeReviews	Gene locus lys9 in contig CU329671_GR.
KEGG	spo:SPBC3B8.03.
NMPDR	fig|4896.1.peg.2277.
Organism-specific databases
GeneDB_Spombe	SPBC3B8.03.
Phylogenomic databases
eggNOG	fuNOG05861.
HOGENOM	HBG324692.
OMA	PYFIYPG.
OrthoDB	EOG9576Q0.
PhylomeDB	O59711.
Enzyme and pathway databases
BRENDA	1.5.1.10. 653.
Gene expression databases
ArrayExpress	O59711.
Family and domain databases
InterPro	IPR016040. NAD(P)-bd_dom. IPR005097. Saccharopine_DH/HSpermid_syn. [Graphical view]
Pfam	PF03435. Saccharop_dh. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

LYS9_SCHPO

Accession

Primary (citable) accession number: O59711

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 5, 2008
Last sequence update:	August 1, 1998
Last modified:	February 9, 2010
This is version 45 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

FPAP (Fungal Proteome Annotation Project)