Reviewed, UniProtKB/Swiss-Prot O59710 (ADRO_SCHPO)

Names and origin

Protein names

Recommended name:
    Probable NADPH:adrenodoxin oxidoreductase, mitochondrial
      Short name=Adrenodoxin reductase
      Short name=AR
    EC=1.18.1.2
Alternative name(s):
    Ferredoxin--NADP(+) reductase
      Short name=Ferredoxin reductase

Gene names

Name:	arh1
ORF Names:	SPBC3B8.01c

Organism

Schizosaccharomyces pombe (Fission yeast) [Complete proteome]

Taxonomic identifier

4896 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Taphrinomycotina › Schizosaccharomycetes › Schizosaccharomycetales › Schizosaccharomycetaceae › Schizosaccharomyces

Protein attributes

Sequence length	469 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Serves as the first electron transfer protein in all the mitochondrial P450 systems By similarity.
Catalytic activity	2 reduced ferredoxin + NADP+ + H+ = 2 oxidized ferredoxin + NADPH.
Cofactor	FAD By similarity.
Subcellular location	Mitochondrion matrix By similarity.
Sequence similarities	Belongs to the ferredoxin--NADP reductase type 1 family.

Ontologies

Keywords
Biological process	Electron transport Transport
Cellular component	Mitochondrion
Domain	Transit peptide
Ligand	FAD Flavoprotein NADP
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	electron transport chain Inferred from electronic annotation. Source: UniProtKB-KW transport Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	mitochondrial matrix Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	FAD binding Inferred from direct assay. Source: GeneDB_SPombe NADP or NADPH binding Inferred from direct assay. Source: GeneDB_SPombe NADPH-adrenodoxin reductase activity Inferred from direct assay. Source: GeneDB_SPombe electron carrier activity Inferred from electronic annotation. Source: InterPro ferredoxin-NADP+ reductase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Transit peptide	1 – 38	38	Mitochondrion Potential
	Chain	39 – 469	431	Probable NADPH:adrenodoxin oxidoreductase, mitochondrial		PRO_0000337259
Regions
	Nucleotide binding	164 – 167	4	NADP By similarity
	Nucleotide binding	208 – 209	2	NADP By similarity
	Nucleotide binding	382 – 384	3	FAD By similarity
Sites
	Binding site	27	1	FAD; via amide nitrogen By similarity
	Binding site	48	1	FAD By similarity
	Binding site	56	1	FAD; via amide nitrogen By similarity
	Binding site	92	1	FAD; via amide nitrogen and carbonyl oxygen By similarity
	Binding site	220	1	NADP By similarity
	Binding site	375	1	FAD; via amide nitrogen By similarity
	Binding site	382	1	NADP; via amide nitrogen By similarity

Sequences

	Sequence		Length	Mass (Da)
	O59710-1 [UniParc]. Last modified August 1, 1998. Version 1. Checksum: 2AFBDBCF86C57291	FASTA	469	52,716
	10 20 30 40 50 60 MLSRFIKRTY STQTSSPVVG IIGSGPAAFY TAHRLLRNDP NVKIDMFESR PVPFGLVRYG 70 80 90 100 110 120 VAPDHPEVKH VEHKFSEIAE STQFRFLGNV NVGTDVSLRD LTKNYDCLVL AYGAAGDKRL 130 140 150 160 170 180 GIPGEDLSGV YSAREVVGWY NSDPRNQNLE LDLSQVEDAV VIGHGNVSLD VARILLSNPA 190 200 210 220 230 240 QLSPTDINPL FLKSLERSNL KRLHIVGRRN IFSVSFTIKE LRELFALSSA VFFAPSFNYS 250 260 270 280 290 300 TKWMNETDAS GLDRPRKRLL KLLVSEIQKA VSEKRVAPYS KDKKCWNLEF GLTPVEILGH 310 320 330 340 350 360 KGNVENVRFQ ITDSIRTDAE SKFTTIPAQL FIRSIGYKSM PLPGMKDVGV PFDDAKGIVK 370 380 390 400 410 420 NVNGFVRPGI YTSGWVKHGP IGVIATTMMD AFATADTITK DWKSKKEFLK NSKLGWDGLK 430 440 450 460 KNIKTPVIHW KDWKVIRNAE IERGLRHESL SEKFRSNEDM IKLIYPGKK

References

[1]

"The genome sequence of Schizosaccharomyces pombe." Wood V., Gwilliam R., Rajandream M.A., Lyne M.H., Lyne R., Stewart A., Sgouros J.G., Peat N., Hayles J., Baker S.G., Basham D., Bowman S., Brooks K., Brown D., Brown S., Chillingworth T., Churcher C.M., Collins M. , Connor R., Cronin A., Davis P., Feltwell T., Fraser A., Gentles S., Goble A., Hamlin N., Harris D.E., Hidalgo J., Hodgson G., Holroyd S., Hornsby T., Howarth S., Huckle E.J., Hunt S., Jagels K., James K.D., Jones L., Jones M., Leather S., McDonald S., McLean J., Mooney P., Moule S., Mungall K.L., Murphy L.D., Niblett D., Odell C., Oliver K., O'Neil S., Pearson D., Quail M.A., Rabbinowitsch E., Rutherford K.M., Rutter S., Saunders D., Seeger K., Sharp S., Skelton J., Simmonds M.N., Squares R., Squares S., Stevens K., Taylor K., Taylor R.G., Tivey A., Walsh S.V., Warren T., Whitehead S., Woodward J.R., Volckaert G., Aert R., Robben J., Grymonprez B., Weltjens I., Vanstreels E., Rieger M., Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Duesterhoeft A., Fritzc C., Holzer E., Moestl D., Hilbert H., Borzym K., Langer I., Beck A., Lehrach H., Reinhardt R., Pohl T.M., Eger P., Zimmermann W., Wedler H., Wambutt R., Purnelle B., Goffeau A., Cadieu E., Dreano S., Gloux S., Lelaure V., Mottier S., Galibert F., Aves S.J., Xiang Z., Hunt C., Moore K., Hurst S.M., Lucas M., Rochet M., Gaillardin C., Tallada V.A., Garzon A., Thode G., Daga R.R., Cruzado L., Jimenez J., Sanchez M., del Rey F., Benito J., Dominguez A., Revuelta J.L., Moreno S., Armstrong J., Forsburg S.L., Cerutti L., Lowe T., McCombie W.R., Paulsen I., Potashkin J., Shpakovski G.V., Ussery D., Barrell B.G., Nurse P. Nature 415:871-880(2002) [PubMed: 11859360] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 38366 / 972.

[2]

"ORFeome cloning and global analysis of protein localization in the fission yeast Schizosaccharomyces pombe." Matsuyama A., Arai R., Yashiroda Y., Shirai A., Kamata A., Sekido S., Kobayashi Y., Hashimoto A., Hamamoto M., Hiraoka Y., Horinouchi S., Yoshida M. Nat. Biotechnol. 24:841-847(2006) [PubMed: 16823372] [Abstract] Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].

Cross-references

Sequence databases
EMBLGenBankDDBJ	CU329671 Genomic DNA. Translation: CAA18290.1.
PIR	T40339.
RefSeq	NP_596413.1.
3D structure databases
HSSP	HSSP built from PDB template 1LQT based on UniProtKB O05783.
ModBase	Search...
Protein-protein interaction databases
STRING	O59710.
Genome annotation databases
GeneID	2541049.
GenomeReviews	Gene locus arh1 in contig CU329671_GR.
KEGG	spo:SPBC3B8.01c.
NMPDR	fig|4896.1.peg.2279.
Organism-specific databases
GeneDB_Spombe	SPBC3B8.01c.
Phylogenomic databases
OMA	YHAVLLT.
Gene expression databases
ArrayExpress	O59710.
Family and domain databases
InterPro	IPR000759. Adrndx_reductase. IPR013027. FAD_pyr_nucl-diS_OxRdtase. [Graphical view]
Pfam	PF07992. Pyr_redox_2. 1 hit. [Graphical view]
PRINTS	PR00419. ADXRDTASE.
ProtoNet	Search...

Entry information

Entry name

ADRO_SCHPO

Accession

Primary (citable) accession number: O59710

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 20, 2008
Last sequence update:	August 1, 1998
Last modified:	November 3, 2009
This is version 53 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

FPAP (Fungal Proteome Annotation Project)