O59702 (CLR6_SCHPO) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Histone deacetylase clr6
EC=3.5.1.98

Alternative name(s):
Cryptic loci regulator 6

Gene names

Name:	clr6
ORF Names:	SPBC36.05c

Organism

Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast) [Reference proteome]

Taxonomic identifier

284812 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Fungi › Dikarya › Ascomycota › Taphrinomycotina › Schizosaccharomycetes › Schizosaccharomycetales › Schizosaccharomycetaceae › Schizosaccharomyces › Schizosaccharomyces pombe

Protein attributes

Sequence length	405 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Responsible for the deacetylation of lysine residues on the N-terminal part of the core histones (H2A, H2B, H3 and H4). Histone deacetylation gives a tag for epigenetic repression and plays an important role in transcriptional regulation, cell cycle progression and developmental events. Histone deacetylases act via the formation of large multiprotein complexes. Has a role in chromatin assembly and chromosome segregation. Ref.3
Catalytic activity	Hydrolysis of an N(6)-acetyl-lysine residue of a histone to yield a deacetylated histone.
Subunit structure	Heterotetramer of alp13, clr6, prw1 and pst2. Ref.3
Subcellular location	Nucleus Ref.3.
Sequence similarities	Belongs to the histone deacetylase family. HD type 1 subfamily.

Ontologies

Keywords
Biological process	Transcription Transcription regulation
Cellular component	Nucleus
Molecular function	Chromatin regulator Hydrolase Repressor
Technical term	Complete proteome Direct protein sequencing Reference proteome
Gene Ontology (GO)
Biological_process	chromatin remodeling Non-traceable author statement. Source: PomBase chromatin silencing at centromere Inferred from mutant phenotype Ref.1. Source: PomBase chromatin silencing at silent mating-type cassette Inferred from genetic interaction Ref.1. Source: PomBase chromosome condensation Inferred from mutant phenotype Ref.3. Source: PomBase histone H4 deacetylation Inferred from electronic annotation. Source: GOC mitotic sister chromatid segregation Traceable author statement PubMed 12526748. Source: PomBase negative regulation of transcription from RNA polymerase II promoter Inferred from genetic interaction PubMed 16407326. Source: PomBase positive regulation of transcription from RNA polymerase II promoter Inferred from mutant phenotype PubMed 20404084. Source: PomBase regulation of histone H3-K9 methylation Non-traceable author statement. Source: PomBase transcription, DNA-dependent Inferred from electronic annotation. Source: UniProtKB-KW
Cellular_component	Rpd3L complex Inferred from direct assay PubMed 17450151. Source: PomBase Rpd3L-Expanded complex Inferred from direct assay PubMed 19040720. Source: PomBase Rpd3S complex Inferred from direct assay Ref.3PubMed 19040720. Source: PomBase cytosol Inferred from direct assay PubMed 16823372. Source: PomBase
Molecular_function	NAD-dependent histone deacetylase activity (H3-K14 specific) Inferred from electronic annotation. Source: EC NAD-dependent histone deacetylase activity (H3-K18 specific) Inferred from electronic annotation. Source: EC NAD-dependent histone deacetylase activity (H3-K9 specific) Inferred from electronic annotation. Source: EC NAD-dependent histone deacetylase activity (H4-K16 specific) Inferred from electronic annotation. Source: EC histone deacetylase activity (H3-K9 specific) Non-traceable author statement. Source: PomBase
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
pst2	O13919	5	EBI-904651,EBI-904686

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 405	405	Histone deacetylase clr6		PRO_0000114740
Regions
	Region	6 – 318	313	Histone deacetylase
Sites
	Active site	138	1	By similarity

Sequences

	Sequence		Length	Mass (Da)
	O59702 [UniParc]. Last modified August 1, 1998. Version 1. Checksum: 8EDEA43D5839E367	FASTA	405	46,112
	10 20 30 40 50 60 MGFGKKKVSY FYDEDVGNYH YGPQHPMKPH RVRMVHNLVV NYNLYEKLNV ITPVRATRND 70 80 90 100 110 120 MTRCHTDEYI EFLWRVTPDT MEKFQPHQLK FNVGDDCPVF DGLYEFCSIS AGGSIGAAQE 130 140 150 160 170 180 LNSGNAEIAI NWAGGLHHAK KREASGFCYV NDIALAALEL LKYHQRVLYI DIDVHHGDGV 190 200 210 220 230 240 EEFFYTTDRV MTCSFHKFGE YFPGTGHIKD TGIGTGKNYA VNVPLRDGID DESYESVFKP 250 260 270 280 290 300 VISHIMQWFR PEAVILQCGT DSLAGDRLGC FNLSMKGHSM CVDFVKSFNL PMICVGGGGY 310 320 330 340 350 360 TVRNVARVWT YETGLLAGEE LDENLPYNDY LQYYGPDYKL NVLSNNMENH NTRQYLDSIT 370 380 390 400 SEIIENLRNL SFAPSVQMHK TPGDFTFENA EKQNIAKEEI MDERV

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Histone deacetylase homologs regulate epigenetic inheritance of transcriptional silencing and chromosome segregation in fission yeast." Grewal S.I.S., Bonaduce M.J., Klar A.J.S. Genetics 150:563-576(1998) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: 972 / ATCC 24843.
[2]	"The genome sequence of Schizosaccharomyces pombe." Wood V., Gwilliam R., Rajandream M.A., Lyne M.H., Lyne R., Stewart A., Sgouros J.G., Peat N., Hayles J., Baker S.G., Basham D., Bowman S., Brooks K., Brown D., Brown S., Chillingworth T., Churcher C.M., Collins M. , Connor R., Cronin A., Davis P., Feltwell T., Fraser A., Gentles S., Goble A., Hamlin N., Harris D.E., Hidalgo J., Hodgson G., Holroyd S., Hornsby T., Howarth S., Huckle E.J., Hunt S., Jagels K., James K.D., Jones L., Jones M., Leather S., McDonald S., McLean J., Mooney P., Moule S., Mungall K.L., Murphy L.D., Niblett D., Odell C., Oliver K., O'Neil S., Pearson D., Quail M.A., Rabbinowitsch E., Rutherford K.M., Rutter S., Saunders D., Seeger K., Sharp S., Skelton J., Simmonds M.N., Squares R., Squares S., Stevens K., Taylor K., Taylor R.G., Tivey A., Walsh S.V., Warren T., Whitehead S., Woodward J.R., Volckaert G., Aert R., Robben J., Grymonprez B., Weltjens I., Vanstreels E., Rieger M., Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Duesterhoeft A., Fritzc C., Holzer E., Moestl D., Hilbert H., Borzym K., Langer I., Beck A., Lehrach H., Reinhardt R., Pohl T.M., Eger P., Zimmermann W., Wedler H., Wambutt R., Purnelle B., Goffeau A., Cadieu E., Dreano S., Gloux S., Lelaure V., Mottier S., Galibert F., Aves S.J., Xiang Z., Hunt C., Moore K., Hurst S.M., Lucas M., Rochet M., Gaillardin C., Tallada V.A., Garzon A., Thode G., Daga R.R., Cruzado L., Jimenez J., Sanchez M., del Rey F., Benito J., Dominguez A., Revuelta J.L., Moreno S., Armstrong J., Forsburg S.L., Cerutti L., Lowe T., McCombie W.R., Paulsen I., Potashkin J., Shpakovski G.V., Ussery D., Barrell B.G., Nurse P. Nature 415:871-880(2002) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: 972 / ATCC 24843.
[3]	"Alp13, an MRG family protein, is a component of fission yeast Clr6 histone deacetylase required for genomic integrity." Nakayama J., Xiao G., Noma K., Malikzay A., Bjerling P., Ekwall K., Kobayashi R., Grewal S.I.S. EMBO J. 22:2776-2787(2003) [PubMed] [Europe PMC] [Abstract] Cited for: PROTEIN SEQUENCE OF 340-368 AND 381-404, FUNCTION, SUBUNIT, SUBCELLULAR LOCATION.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF064206 Genomic DNA. Translation: AAD05211.1. CU329671 Genomic DNA. Translation: CAA19053.1.
PIR	T40300.
RefSeq	NP_595333.1. NM_001021241.2.
3D structure databases
ProteinModelPortal	O59702.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-29339N.
IntAct	O59702. 4 interactions.
STRING	4896.SPBC36.05c-1.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	SPBC36.05c.1; SPBC36.05c.1:pep; SPBC36.05c.
GeneID	2540368.
KEGG	spo:SPBC36.05c.
Organism-specific databases
PomBase	SPBC36.05c.
Phylogenomic databases
eggNOG	COG0123.
HOGENOM	HOG000225180.
KO	K06067.
OMA	RISCDEE.
OrthoDB	EOG4RV60J.
Family and domain databases
Gene3D	3.40.800.20. 1 hit.
InterPro	IPR000286. His_deacetylse. IPR003084. His_deacetylse_1. IPR023801. His_deacetylse_dom. [Graphical view]
PANTHER	PTHR10625. PTHR10625. 1 hit.
Pfam	PF00850. Hist_deacetyl. 1 hit. [Graphical view]
PIRSF	PIRSF037913. His_deacetylse_1. 1 hit.
PRINTS	PR01270. HDASUPER. PR01271. HISDACETLASE.
ProtoNet	Search...
Other
NextBio	20801496.

Entry information

Entry name

CLR6_SCHPO

Accession

Primary (citable) accession number: O59702

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 25, 2003
Last sequence update:	August 1, 1998
Last modified:	May 1, 2013
This is version 98 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program