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Protein

Cysteine synthase 1

Gene

cys11

Organism
Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Catalyzes the conversion of O-succinyl-L-serine into cysteine, the last step in the cysteine biosynthesis pathway (PubMed:28581482). Can also use O-acetyl-L-serine (PubMed:17482430, PubMed:28581482).1 Publication1 Publication

Catalytic activityi

O-succinyl-L-serine + hydrogen sulfide = L-cysteine + succinate.1 Publication
O-acetyl-L-serine + hydrogen sulfide = L-cysteine + acetate.2 Publications

Cofactori

pyridoxal 5'-phosphateBy similarity

Kineticsi

kcat is 2.8 sec(-1) with O-succinyl-L-serine as substrate. kcat is 1.5 sec(-1) with O-acetyl-L-serine as substrate.1 Publication
  1. KM=0.24 mM for O-succinyl-L-serine1 Publication
  2. KM=0.33 mM for O-acetyl-L-serine1 Publication
  3. KM=0.34 mM for Na2S1 Publication

    Pathwayi: L-cysteine biosynthesis

    This protein is involved in step 2 of the subpathway that synthesizes L-cysteine from L-serine.2 Publications
    Proteins known to be involved in the 2 steps of the subpathway in this organism are:
    1. Serine O-succinyltransferase (cys2), Putative serine acetyltransferase (SPAC1039.08)
    2. Cysteine synthase 1 (cys11)
    This subpathway is part of the pathway L-cysteine biosynthesis, which is itself part of Amino-acid biosynthesis.
    View all proteins of this organism that are known to be involved in the subpathway that synthesizes L-cysteine from L-serine, the pathway L-cysteine biosynthesis and in Amino-acid biosynthesis.

    Sites

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Binding sitei84Pyridoxal phosphateBy similarity1
    Binding sitei289Pyridoxal phosphateBy similarity1

    GO - Molecular functioni

    • cysteine synthase activity Source: PomBase
    • pyridoxal phosphate binding Source: GO_Central

    GO - Biological processi

    • cysteine biosynthetic process from serine Source: PomBase

    Keywordsi

    Molecular functionTransferase
    Biological processAmino-acid biosynthesis, Cysteine biosynthesis
    LigandPyridoxal phosphate

    Enzyme and pathway databases

    UniPathwayiUPA00136; UER00200

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Cysteine synthase 11 Publication (EC:2.5.1.-1 Publication, EC:2.5.1.472 Publications)
    Short name:
    CS 1
    Alternative name(s):
    O-acetylserine (thiol)-lyase 1
    Short name:
    OAS-TL 1
    O-acetylserine sulfhydrylase 1
    O-succinylserine sulfhydrylaseCurated
    Gene namesi
    Name:cys11
    Synonyms:cys1a1 Publication
    ORF Names:SPBC36.04Imported
    OrganismiSchizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
    Taxonomic identifieri284812 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaTaphrinomycotinaSchizosaccharomycetesSchizosaccharomycetalesSchizosaccharomycetaceaeSchizosaccharomyces
    Proteomesi
    • UP000002485 Componenti: Chromosome II

    Organism-specific databases

    EuPathDBiFungiDB:SPBC36.04
    PomBaseiSPBC36.04 cys11

    Subcellular locationi

    Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

    Keywords - Cellular componenti

    Mitochondrion

    Pathology & Biotechi

    Disruption phenotypei

    Causes cysteine auxotrophy.1 Publication

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Transit peptidei1 – 29MitochondrionSequence analysisAdd BLAST29
    ChainiPRO_000016712830 – 351Cysteine synthase 1Add BLAST322

    Amino acid modifications

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Modified residuei54N6-(pyridoxal phosphate)lysineBy similarity1

    Proteomic databases

    MaxQBiO59701
    PaxDbiO59701
    PRIDEiO59701

    PTM databases

    iPTMnetiO59701

    Interactioni

    Protein-protein interaction databases

    BioGridi277462, 52 interactors
    STRINGi4896.SPBC36.04.1

    Structurei

    3D structure databases

    ProteinModelPortaliO59701
    SMRiO59701
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Region

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Regioni190 – 194Pyridoxal phosphate bindingBy similarity5

    Sequence similaritiesi

    Keywords - Domaini

    Transit peptide

    Phylogenomic databases

    HOGENOMiHOG000217393
    InParanoidiO59701
    KOiK01738
    OMAiHKTEGIG
    OrthoDBiEOG092C33M9
    PhylomeDBiO59701

    Family and domain databases

    InterProiView protein in InterPro
    IPR001216 P-phosphate_BS
    IPR001926 PLP-dep
    IPR036052 Trypto_synt_PLP_dependent
    PfamiView protein in Pfam
    PF00291 PALP, 1 hit
    SUPFAMiSSF53686 SSF53686, 1 hit
    PROSITEiView protein in PROSITE
    PS00901 CYS_SYNTHASE, 1 hit

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    O59701-1 [UniParc]FASTAAdd to basket

    « Hide

            10         20         30         40         50
    MATSGIQTKV PGIVSGFIGA IGRTPLIRLN TLSNETGCNI LAKAEFQNPG
    60 70 80 90 100
    GSVKDRAAYY VVRDAEKKGK LSRGGTIVEG TAGNTGIGLA HIARARGYKC
    110 120 130 140 150
    VIYMPNTQSQ AKIDTLKFLG AEVHPVPVAP FSNPLNYNHQ ARRHAESTPN
    160 170 180 190 200
    ASWTDQFDNV ANLLSHYETT GPEIWDQTKG TVDGFTCSTG TGGTFAGVTK
    210 220 230 240 250
    YLKEKSDGRV ASFVADPPGS VLYSHIKTKG KHPDNKGSSF TEGIGQGRIT
    260 270 280 290 300
    GNVQPVYDLI DDAMKIPDEK SINMFFRLLD QEGLFLGGSS CLNVVAAVEM
    310 320 330 340 350
    AKILGPGKTV VTILCDSGHK YATRLFSRSF LESKKLFDVI EPQYKKYIVL

    P
    Length:351
    Mass (Da):37,853
    Last modified:August 1, 1998 - v1
    Checksum:iEF11655CE2BE4A60
    GO

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    CU329671 Genomic DNA Translation: CAA19052.1
    PIRiT40299
    RefSeqiNP_595332.1, NM_001021240.2

    Genome annotation databases

    EnsemblFungiiSPBC36.04.1; SPBC36.04.1:pep; SPBC36.04
    GeneIDi2540946
    KEGGispo:SPBC36.04

    Similar proteinsi

    Entry informationi

    Entry nameiCYSK_SCHPO
    AccessioniPrimary (citable) accession number: O59701
    Entry historyiIntegrated into UniProtKB/Swiss-Prot: March 29, 2005
    Last sequence update: August 1, 1998
    Last modified: March 28, 2018
    This is version 131 of the entry and version 1 of the sequence. See complete history.
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. Schizosaccharomyces pombe
      Schizosaccharomyces pombe: entries and gene names
    3. SIMILARITY comments
      Index of protein domains and families

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