O59701 (CYSK1_SCHPO) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Cysteine synthase 1
Short name=CSase 1
EC=2.5.1.47

Alternative name(s):
O-acetylserine (thiol)-lyase 1
Short name=OAS-TL 1
O-acetylserine sulfhydrylase 1

Gene names

Name:	cys11
Synonyms:	cys1a
ORF Names:	SPBC36.04

Organism

Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)

Taxonomic identifier

284812 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Taphrinomycotina › Schizosaccharomycetes › Schizosaccharomycetales › Schizosaccharomycetaceae › Schizosaccharomyces

Protein attributes

Sequence length	351 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Catalytic activity	O(3)-acetyl-L-serine + H2S = L-cysteine + acetate.
Cofactor	Pyridoxal phosphate By similarity.
Pathway	Amino-acid biosynthesis; L-cysteine biosynthesis; L-cysteine from L-serine: step 2/2.
Sequence similarities	Belongs to the cysteine synthase/cystathionine beta-synthase family.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Cysteine biosynthesis
Ligand	Pyridoxal phosphate
Molecular function	Transferase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	cysteine biosynthetic process from serine Inferred from electronic annotation. Source: InterPro
Cellular component	mitochondrion Inferred from direct assay. Source: GeneDB_Spombe
Molecular function	cysteine synthase activity Inferred from mutant phenotype. Source: GeneDB_Spombe pyridoxal phosphate binding Inferred from electronic annotation. Source: InterPro transferase activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 351	351	Cysteine synthase 1		PRO_0000167128
Regions
	Region	190 – 194	5	Pyridoxal phosphate binding By similarity
Sites
	Binding site	84	1	Pyridoxal phosphate By similarity
	Binding site	289	1	Pyridoxal phosphate By similarity
Amino acid modifications
	Modified residue	54	1	N6-(pyridoxal phosphate)lysine By similarity

Sequences

	Sequence		Length	Mass (Da)
	O59701 [UniParc]. Last modified August 1, 1998. Version 1. Checksum: EF11655CE2BE4A60	FASTA	351	37,853
	10 20 30 40 50 60 MATSGIQTKV PGIVSGFIGA IGRTPLIRLN TLSNETGCNI LAKAEFQNPG GSVKDRAAYY 70 80 90 100 110 120 VVRDAEKKGK LSRGGTIVEG TAGNTGIGLA HIARARGYKC VIYMPNTQSQ AKIDTLKFLG 130 140 150 160 170 180 AEVHPVPVAP FSNPLNYNHQ ARRHAESTPN ASWTDQFDNV ANLLSHYETT GPEIWDQTKG 190 200 210 220 230 240 TVDGFTCSTG TGGTFAGVTK YLKEKSDGRV ASFVADPPGS VLYSHIKTKG KHPDNKGSSF 250 260 270 280 290 300 TEGIGQGRIT GNVQPVYDLI DDAMKIPDEK SINMFFRLLD QEGLFLGGSS CLNVVAAVEM 310 320 330 340 350 AKILGPGKTV VTILCDSGHK YATRLFSRSF LESKKLFDVI EPQYKKYIVL P

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The genome sequence of Schizosaccharomyces pombe." Wood V., Gwilliam R., Rajandream M.A., Lyne M.H., Lyne R., Stewart A., Sgouros J.G., Peat N., Hayles J., Baker S.G., Basham D., Bowman S., Brooks K., Brown D., Brown S., Chillingworth T., Churcher C.M., Collins M. , Connor R., Cronin A., Davis P., Feltwell T., Fraser A., Gentles S., Goble A., Hamlin N., Harris D.E., Hidalgo J., Hodgson G., Holroyd S., Hornsby T., Howarth S., Huckle E.J., Hunt S., Jagels K., James K.D., Jones L., Jones M., Leather S., McDonald S., McLean J., Mooney P., Moule S., Mungall K.L., Murphy L.D., Niblett D., Odell C., Oliver K., O'Neil S., Pearson D., Quail M.A., Rabbinowitsch E., Rutherford K.M., Rutter S., Saunders D., Seeger K., Sharp S., Skelton J., Simmonds M.N., Squares R., Squares S., Stevens K., Taylor K., Taylor R.G., Tivey A., Walsh S.V., Warren T., Whitehead S., Woodward J.R., Volckaert G., Aert R., Robben J., Grymonprez B., Weltjens I., Vanstreels E., Rieger M., Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Duesterhoeft A., Fritzc C., Holzer E., Moestl D., Hilbert H., Borzym K., Langer I., Beck A., Lehrach H., Reinhardt R., Pohl T.M., Eger P., Zimmermann W., Wedler H., Wambutt R., Purnelle B., Goffeau A., Cadieu E., Dreano S., Gloux S., Lelaure V., Mottier S., Galibert F., Aves S.J., Xiang Z., Hunt C., Moore K., Hurst S.M., Lucas M., Rochet M., Gaillardin C., Tallada V.A., Garzon A., Thode G., Daga R.R., Cruzado L., Jimenez J., Sanchez M., del Rey F., Benito J., Dominguez A., Revuelta J.L., Moreno S., Armstrong J., Forsburg S.L., Cerutti L., Lowe T., McCombie W.R., Paulsen I., Potashkin J., Shpakovski G.V., Ussery D., Barrell B.G., Nurse P. Nature 415:871-880(2002) [PubMed: 11859360] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: 972 / ATCC 24843.
[2]	"Characterization of two genes encoding putative cysteine synthase required for cysteine biosynthesis in Schizosaccharomyces pombe." Fujita Y., Takegawa K. Biosci. Biotechnol. Biochem. 68:306-311(2004) [PubMed: 14981292] [Abstract] Cited for: IDENTIFICATION. Strain: ATCC 38399.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CU329671 Genomic DNA. Translation: CAA19052.1.
PIR	T40299.
RefSeq	NP_595332.1. NM_001021240.1.
3D structure databases
ProteinModelPortal	O59701.
ModBase	Search...
Protein-protein interaction databases
STRING	O59701.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	SPBC36.04.1; SPBC36.04.1:pep; SPBC36.04.
GeneID	2540946.
GenomeReviews	Gene locus cys11 in contig CU329671_GR.
KEGG	spo:SPBC36.04.
NMPDR	fig|4896.1.peg.1198.
Organism-specific databases
GeneDB_Spombe	SPBC36.04.
Phylogenomic databases
eggNOG	fuNOG07167.
GeneTree	EFGT00050000001990.
HOGENOM	HBG748215.
OMA	NHQAKRF.
OrthoDB	EOG4SFDFK.
Gene expression databases
ArrayExpress	O59701.
Family and domain databases
InterPro	IPR001216. Cys_synth_BS. IPR001926. PyrdxlP-dep_enz_bsu. [Graphical view]
KO	K01738.
Pfam	PF00291. PALP. 1 hit. [Graphical view]
SUPFAM	SSF53686. PyrdxlP-dep_enz_bsu. 1 hit.
PROSITE	PS00901. CYS_SYNTHASE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

CYSK1_SCHPO

Accession

Primary (citable) accession number: O59701

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 29, 2005
Last sequence update:	August 1, 1998
Last modified:	December 14, 2011
This is version 78 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program