O59667 (HIS2_SCHPO) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Histidine biosynthesis bifunctional protein his7

Including the following 2 domains:

1. Phosphoribosyl-AMP cyclohydrolase
   EC=3.5.4.19
2. Phosphoribosyl-ATP pyrophosphatase
   EC=3.6.1.31

Gene names

Name:	his7
ORF Names:	SPBC29A3.02c

Organism

Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)

Taxonomic identifier

284812 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Taphrinomycotina › Schizosaccharomycetes › Schizosaccharomycetales › Schizosaccharomycetaceae › Schizosaccharomyces

Protein attributes

Sequence length	417 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Catalytic activity	1-(5-phosphoribosyl)-ATP + H2O = 1-(5-phosphoribosyl)-AMP + diphosphate. UniProtKB P00815 1-(5-phosphoribosyl)-AMP + H2O = 1-(5-phosphoribosyl)-5-((5-phosphoribosylamino)methylideneamino)imidazole-4-carboxamide. UniProtKB P00815
Pathway	Amino-acid biosynthesis; L-histidine biosynthesis; L-histidine from 5-phospho-alpha-D-ribose 1-diphosphate: step 2/9. UniProtKB P00815 Amino-acid biosynthesis; L-histidine biosynthesis; L-histidine from 5-phospho-alpha-D-ribose 1-diphosphate: step 3/9.
Subcellular location	Cytoplasm Ref.2.
Caution	In contrast to other fungi which have a single histidine biosynthesis trifunctional protein, 2 proteins are present in S.pombe to ensure these functions: his7 (phosphoribosyl-AMP cyclohydrolase and phosphoribosyl-ATP pyrophosphatase activities) and his2 (histidinol dehydrogenase activity).

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Histidine biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Hydrolase
Technical term	Complete proteome Multifunctional enzyme Reference proteome
Gene Ontology (GO)
Biological process	histidine biosynthetic process Inferred from genetic interaction. Source: GeneDB_Spombe
Cellular component	cytosol Inferred from direct assay. Source: GeneDB_Spombe nucleus Inferred from direct assay. Source: GeneDB_Spombe
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW phosphoribosyl-AMP cyclohydrolase activity Inferred from genetic interaction. Source: GeneDB_Spombe phosphoribosyl-ATP diphosphatase activity Inferred from sequence or structural similarity. Source: GeneDB_Spombe
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 417	417	Histidine biosynthesis bifunctional protein his7		PRO_0000352786
Regions
	Region	225 – 299	75	Phosphoribosyl-AMP cyclohydrolase
	Region	303 – 387	85	Phosphoribosyl-ATP pyrophosphohydrolase

Sequences

	Sequence		Length	Mass (Da)
	O59667 [UniParc]. Last modified August 1, 1998. Version 1. Checksum: F3911725940AFD74	FASTA	417	46,183
	10 20 30 40 50 60 MALLPFFDLT NFESDASEEL GWLKYVGRVQ TRVFPQHFKD NLEKVRKISE TIDVIVDTTA 70 80 90 100 110 120 ELGPEACVNL LNAGALAILV NEEMLNELAD ISPNRLVLKT DTTDIGKIEK LSQVAGSIQW 130 140 150 160 170 180 IGSAENYPPD FFERASKIIH KAVMPEGGGR TLYLEFPEQP SMEVLKSFSV HSVVPVLSSS 190 200 210 220 230 240 FLTVKPAEEP KKLSLADLIL ISANTDREDG LFSTLVVNEL GIALGLVYSS KESVAESLKT 250 260 270 280 290 300 GTGVYQSRKR GLWYKGASSG AVQHLIHIDV DCDEDCLRFV VYQTGKGFCH LDTLHCFGQA 310 320 330 340 350 360 SGLCQLEKTL IDRKNNAPEG SYTARLFSDP KLLRAKIMEE AEELCDATTK ENVIWEMADL 370 380 390 400 410 MYFAITRCVG SGVSLNDISR HLDLKHRKVT RRKGDAKVAW QEKLKDKGGV ANTSYTA

References

[1]

"The genome sequence of Schizosaccharomyces pombe." Wood V., Gwilliam R., Rajandream M.A., Lyne M.H., Lyne R., Stewart A., Sgouros J.G., Peat N., Hayles J., Baker S.G., Basham D., Bowman S., Brooks K., Brown D., Brown S., Chillingworth T., Churcher C.M., Collins M. , Connor R., Cronin A., Davis P., Feltwell T., Fraser A., Gentles S., Goble A., Hamlin N., Harris D.E., Hidalgo J., Hodgson G., Holroyd S., Hornsby T., Howarth S., Huckle E.J., Hunt S., Jagels K., James K.D., Jones L., Jones M., Leather S., McDonald S., McLean J., Mooney P., Moule S., Mungall K.L., Murphy L.D., Niblett D., Odell C., Oliver K., O'Neil S., Pearson D., Quail M.A., Rabbinowitsch E., Rutherford K.M., Rutter S., Saunders D., Seeger K., Sharp S., Skelton J., Simmonds M.N., Squares R., Squares S., Stevens K., Taylor K., Taylor R.G., Tivey A., Walsh S.V., Warren T., Whitehead S., Woodward J.R., Volckaert G., Aert R., Robben J., Grymonprez B., Weltjens I., Vanstreels E., Rieger M., Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Duesterhoeft A., Fritzc C., Holzer E., Moestl D., Hilbert H., Borzym K., Langer I., Beck A., Lehrach H., Reinhardt R., Pohl T.M., Eger P., Zimmermann W., Wedler H., Wambutt R., Purnelle B., Goffeau A., Cadieu E., Dreano S., Gloux S., Lelaure V., Mottier S., Galibert F., Aves S.J., Xiang Z., Hunt C., Moore K., Hurst S.M., Lucas M., Rochet M., Gaillardin C., Tallada V.A., Garzon A., Thode G., Daga R.R., Cruzado L., Jimenez J., Sanchez M., del Rey F., Benito J., Dominguez A., Revuelta J.L., Moreno S., Armstrong J., Forsburg S.L., Cerutti L., Lowe T., McCombie W.R., Paulsen I., Potashkin J., Shpakovski G.V., Ussery D., Barrell B.G., Nurse P. Nature 415:871-880(2002) [PubMed: 11859360] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: 972 / ATCC 24843.

[2]

"ORFeome cloning and global analysis of protein localization in the fission yeast Schizosaccharomyces pombe." Matsuyama A., Arai R., Yashiroda Y., Shirai A., Kamata A., Sekido S., Kobayashi Y., Hashimoto A., Hamamoto M., Hiraoka Y., Horinouchi S., Yoshida M. Nat. Biotechnol. 24:841-847(2006) [PubMed: 16823372] [Abstract] Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].

Cross-references

Sequence databases
EMBL GenBank DDBJ	CU329671 Genomic DNA. Translation: CAA18379.1.
PIR	T40073.
RefSeq	NP_595830.1. NM_001021734.1.
3D structure databases
HSSP	HSSP built from PDB template 1ZPS based on UniProtKB O26347.
ProteinModelPortal	O59667.
ModBase	Search...
Protein-protein interaction databases
STRING	O59667.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	SPBC29A3.02c.1; SPBC29A3.02c.1:pep; SPBC29A3.02c.
GeneID	2540290.
GenomeReviews	Gene locus his7 in contig CU329671_GR.
KEGG	spo:SPBC29A3.02c.
NMPDR	fig|4896.1.peg.1696.
Organism-specific databases
GeneDB_Spombe	SPBC29A3.02c.
Phylogenomic databases
eggNOG	fuNOG06008.
GeneTree	EFGT00050000001873.
OrthoDB	EOG4CRQ93.
Gene expression databases
ArrayExpress	O59667.
Family and domain databases
InterPro	IPR008179. PRib-ATP_PPHydrolase. IPR021130. PRib-ATP_PPHydrolase-like. IPR002496. PRib_AMP_CycHydrolase. [Graphical view]
KO	K11755.
Pfam	PF01502. PRA-CH. 1 hit. PF01503. PRA-PH. 1 hit. [Graphical view]
ProDom	PD002610. PRA_CycHdrlase. 1 hit. [Graphical view] [Entries sharing at least one domain]
TIGRFAMs	TIGR03188. Histidine_hisI. 1 hit.
ProtoNet	Search...

Entry information

Entry name

HIS2_SCHPO

Accession

Primary (citable) accession number: O59667

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 14, 2008
Last sequence update:	August 1, 1998
Last modified:	December 14, 2011
This is version 66 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program