Reviewed, UniProtKB/Swiss-Prot O59650 (DHE3_PYRKO)

Names and origin

Protein names

Recommended name:
    Glutamate dehydrogenase
      Short name=GDH
    EC=1.4.1.3

Gene names

Name:	gdhA
Ordered Locus Names:	TK1431

Organism

Pyrococcus kodakaraensis (Thermococcus kodakaraensis) [Complete proteome] [HAMAP]

Taxonomic identifier

69014 [NCBI]

Taxonomic lineage

Archaea › Euryarchaeota › Thermococci › Thermococcales › Thermococcaceae › Thermococcus

Protein attributes

Sequence length	421 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level.

General annotation (Comments)

Catalytic activity	L-glutamate + H(2)O + NAD(P)(+) = 2-oxoglutarate + NH(3) + NAD(P)H.
Subunit structure	Homohexamer.
Subcellular location	Cytoplasm.
Miscellaneous	Uses both NAD and NADP.
Sequence similarities	Belongs to the Glu/Leu/Phe/Val dehydrogenases family.

Ontologies

Keywords
Cellular component	Cytoplasm
Ligand	NAD NADP
Molecular function	Oxidoreductase
Technical term	Complete proteome Direct protein sequencing
Gene Ontology (GO)
Biological process	amino acid metabolic process Inferred from electronic annotation. Source: InterPro oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	binding Inferred from electronic annotation. Source: InterPro glutamate dehydrogenase [NAD(P)+] activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Initiator methionine	1	1	Removed
	Chain	2 – 421	420	Glutamate dehydrogenase		PRO_0000182759
Regions
	Nucleotide binding	220 – 226	7	NAD Potential
Sites
	Active site	105	1	By similarity
Experimental info
	Sequence conflict	138	1	W → C in BAA25261. Ref.1
	Sequence conflict	347	1	A → T in BAA25261. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	O59650-1 [UniParc]. Last modified January 23, 2007. Version 4. Checksum: 13EF3F1DD1602E51	FASTA	421	47,056
	10 20 30 40 50 60 MVEIDPFEMA VQQLERAAQF MDISEEALEW LKRPMRIVEV SVPVEMDDGS VKVFTGFRVQ 70 80 90 100 110 120 HNWARGPTKG GIRWHPAETL STVKALATWM TWKVAVVDLP YGGGKGGIIV DPKKLSEREQ 130 140 150 160 170 180 ERLARSYIRA VYDVIGPWTD IPAPDVYTNP KIMAWMMDEY ETIMRRKGPA FGVITGKPPG 190 200 210 220 230 240 VGGIVARMDA TARGAAFTIR EAAKALGWDD LKGKTIAIQG YGNAGYYLHK IMSEEFGMKV 250 260 270 280 290 300 VAVSDSKGGI YNPDGLPPAD EVLKWKKEHG SVKDMPGTQN ITNEELLELE VDILAPSAIE 310 320 330 340 350 360 GVITKENADN VKAKIVAEVA NGPVTPEADE ILHEKGILQI PDFLCNAGGV TVSYFEWVQN 370 380 390 400 410 420 INGFYWTVEE TRKRLDDKMT KAFWDVFNTH KEKNIHMRDA AYVVAVSRVY EAMKHRGWVK K

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Sequence analysis of glutamate dehydrogenase (GDH) from the hyperthermophilic archaeon Pyrococcus sp. KOD1 and comparison of the enzymatic characteristics of native and recombinant GDHs." Rahman R.N.Z.A., Fujiwara S., Takagi M., Imanaka T. Mol. Gen. Genet. 257:338-347(1998) [PubMed: 9520268] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 2-11. Strain: KOD1.
[2]	"Complete genome sequence of the hyperthermophilic archaeon Thermococcus kodakaraensis KOD1 and comparison with Pyrococcus genomes." Fukui T., Atomi H., Kanai T., Matsumi R., Fujiwara S., Imanaka T. Genome Res. 15:352-363(2005) [PubMed: 15710748] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: KOD1.

Cross-references

Sequence databases
EMBLGenBankDDBJ	D89911 Genomic DNA. Translation: BAA25261.1. AP006878 Genomic DNA. Translation: BAD85620.1.
PIR	T44789.
RefSeq	YP_183844.1.
3D structure databases
HSSP	HSSP built from PDB template 1EUZ based on UniProtKB O74024.
SMR	O59650. Positions 5-420.
ModBase	Search...
Genome annotation databases
GeneID	3234478.
GenomeReviews	Gene locus TK1431 in contig AP006878_GR.
KEGG	tko:TK1431.
NMPDR	fig|69014.3.peg.1302.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	O59650.
Enzyme and pathway databases
BioCyc	TKOD69014:TK1431-MON.
Family and domain databases
InterPro	IPR006095. Glu/Leu/Phe/Val_DHase. IPR006096. Glu/Leu/Phe/Val_DHase_C. IPR006097. Glu/Leu/Phe/Val_DHase_dimer. IPR014362. Glu_DHase. IPR016040. NAD(P)-bd. [Graphical view]
Gene3D	G3DSA:3.40.50.720. NAD(P)-bd. 1 hit.
PANTHER	PTHR11606:SF2. GLFV_DH. 1 hit.
Pfam	PF00208. ELFV_dehydrog. 1 hit. PF02812. ELFV_dehydrog_N. 1 hit. [Graphical view]
PIRSF	PIRSF000185. Glu_DH. 1 hit.
PRINTS	PR00082. GLFDHDRGNASE.
PROSITE	PS00074. GLFV_DEHYDROGENASE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

DHE3_PYRKO

Accession

Primary (citable) accession number: O59650
Secondary accession number(s): Q5JDK3

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 15, 1998
Last sequence update:	January 23, 2007
Last modified:	November 25, 2008
This is version 55 of the entry and version 4 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)