O59650 (DHE3_PYRKO) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Glutamate dehydrogenase
Short name=GDH
EC=1.4.1.3

Gene names

Name:	gdhA
Ordered Locus Names:	TK1431

Organism

Pyrococcus kodakaraensis (strain ATCC BAA-918 / JCM 12380 / KOD1) (Thermococcus kodakaraensis (strain KOD1))

Taxonomic identifier

69014 [NCBI]

Taxonomic lineage

Archaea › Euryarchaeota › Thermococci › Thermococcales › Thermococcaceae › Thermococcus

Protein attributes

Sequence length	421 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Catalytic activity	L-glutamate + H2O + NAD(P)+ = 2-oxoglutarate + NH3 + NAD(P)H.
Subunit structure	Homohexamer.
Subcellular location	Cytoplasm.
Miscellaneous	Uses both NAD and NADP.
Sequence similarities	Belongs to the Glu/Leu/Phe/Val dehydrogenases family.

Ontologies

Keywords
Cellular component	Cytoplasm
Ligand	NAD NADP
Molecular function	Oxidoreductase
Technical term	Complete proteome Direct protein sequencing Reference proteome
Gene Ontology (GO)
Biological process	cellular amino acid metabolic process Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	glutamate dehydrogenase [NAD(P)+] activity Inferred from electronic annotation. Source: EC nucleotide binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Initiator methionine	1	1	Removed Ref.1
	Chain	2 – 421	420	Glutamate dehydrogenase		PRO_0000182759
Regions
	Nucleotide binding	220 – 226	7	NAD Potential
Sites
	Active site	105	1	By similarity
Experimental info
	Sequence conflict	138	1	W → C in BAA25261. Ref.1
	Sequence conflict	347	1	A → T in BAA25261. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	O59650 [UniParc]. Last modified January 23, 2007. Version 4. Checksum: 13EF3F1DD1602E51	FASTA	421	47,056
	10 20 30 40 50 60 MVEIDPFEMA VQQLERAAQF MDISEEALEW LKRPMRIVEV SVPVEMDDGS VKVFTGFRVQ 70 80 90 100 110 120 HNWARGPTKG GIRWHPAETL STVKALATWM TWKVAVVDLP YGGGKGGIIV DPKKLSEREQ 130 140 150 160 170 180 ERLARSYIRA VYDVIGPWTD IPAPDVYTNP KIMAWMMDEY ETIMRRKGPA FGVITGKPPG 190 200 210 220 230 240 VGGIVARMDA TARGAAFTIR EAAKALGWDD LKGKTIAIQG YGNAGYYLHK IMSEEFGMKV 250 260 270 280 290 300 VAVSDSKGGI YNPDGLPPAD EVLKWKKEHG SVKDMPGTQN ITNEELLELE VDILAPSAIE 310 320 330 340 350 360 GVITKENADN VKAKIVAEVA NGPVTPEADE ILHEKGILQI PDFLCNAGGV TVSYFEWVQN 370 380 390 400 410 420 INGFYWTVEE TRKRLDDKMT KAFWDVFNTH KEKNIHMRDA AYVVAVSRVY EAMKHRGWVK K

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Sequence analysis of glutamate dehydrogenase (GDH) from the hyperthermophilic archaeon Pyrococcus sp. KOD1 and comparison of the enzymatic characteristics of native and recombinant GDHs." Rahman R.N.Z.A., Fujiwara S., Takagi M., Imanaka T. Mol. Gen. Genet. 257:338-347(1998) [PubMed: 9520268] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 2-11. Strain: ATCC BAA-918 / JCM 12380 / KOD1.
[2]	"Complete genome sequence of the hyperthermophilic archaeon Thermococcus kodakaraensis KOD1 and comparison with Pyrococcus genomes." Fukui T., Atomi H., Kanai T., Matsumi R., Fujiwara S., Imanaka T. Genome Res. 15:352-363(2005) [PubMed: 15710748] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC BAA-918 / JCM 12380 / KOD1.

Cross-references

Sequence databases
EMBL GenBank DDBJ	D89911 Genomic DNA. Translation: BAA25261.1. AP006878 Genomic DNA. Translation: BAD85620.1.
PIR	T44789.
RefSeq	YP_183844.1. NC_006624.1.
3D structure databases
ProteinModelPortal	O59650.
SMR	O59650. Positions 3-421.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	EBPYRT00000006258; EBPYRP00000006039; EBPYRG00000006257.
GeneID	3234478.
GenomeReviews	Gene locus TK1431 in contig AP006878_GR.
KEGG	tko:TK1431.
NMPDR	fig|69014.3.peg.1302.
Phylogenomic databases
eggNOG	arNOG04583.
GeneTree	EBGT00050000022667.
HOGENOM	HBG590661.
OMA	NIHMRDA.
PhylomeDB	O59650.
ProtClustDB	CLSK434170.
Enzyme and pathway databases
BioCyc	TKOD69014:TK1431-MONOMER.
Family and domain databases
InterPro	IPR006095. Glu/Leu/Phe/Val_DH. IPR006096. Glu/Leu/Phe/Val_DH_C. IPR006097. Glu/Leu/Phe/Val_DH_dimer_dom. IPR014362. Glu_DH. IPR016040. NAD(P)-bd_dom. [Graphical view]
Gene3D	G3DSA:3.40.50.720. NAD(P)-bd. 1 hit.
KO	K00261.
Pfam	PF00208. ELFV_dehydrog. 1 hit. PF02812. ELFV_dehydrog_N. 1 hit. [Graphical view]
PIRSF	PIRSF000185. Glu_DH. 1 hit.
PRINTS	PR00082. GLFDHDRGNASE.
SMART	SM00839. ELFV_dehydrog. 1 hit. [Graphical view]
PROSITE	PS00074. GLFV_DEHYDROGENASE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

DHE3_PYRKO

Accession

Primary (citable) accession number: O59650
Secondary accession number(s): Q5JDK3

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 15, 1998
Last sequence update:	January 23, 2007
Last modified:	December 14, 2011
This is version 80 of the entry and version 4 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program