Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.

O59650

- DHE3_THEKO

UniProt

O59650 - DHE3_THEKO

(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

Protein

Glutamate dehydrogenase

Gene

gdhA

Organism
Thermococcus kodakaraensis (strain ATCC BAA-918 / JCM 12380 / KOD1) (Pyrococcus kodakaraensis (strain KOD1))
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at protein leveli

Functioni

Catalytic activityi

L-glutamate + H2O + NAD(P)+ = 2-oxoglutarate + NH3 + NAD(P)H.PROSITE-ProRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei105 – 1051PROSITE-ProRule annotation

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi220 – 2267NADSequence Analysis

GO - Molecular functioni

  1. glutamate dehydrogenase [NAD(P)+] activity Source: UniProtKB-EC

GO - Biological processi

  1. cellular amino acid metabolic process Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Ligandi

NAD, NADP

Enzyme and pathway databases

BioCyciTKOD69014:GH72-1456-MONOMER.
SABIO-RKO59650.

Names & Taxonomyi

Protein namesi
Recommended name:
Glutamate dehydrogenase (EC:1.4.1.3)
Short name:
GDH
Gene namesi
Name:gdhA
Ordered Locus Names:TK1431
OrganismiThermococcus kodakaraensis (strain ATCC BAA-918 / JCM 12380 / KOD1) (Pyrococcus kodakaraensis (strain KOD1))
Taxonomic identifieri69014 [NCBI]
Taxonomic lineageiArchaeaEuryarchaeotaThermococciThermococcalesThermococcaceaeThermococcus
ProteomesiUP000000536: Chromosome

Subcellular locationi

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methioninei1 – 11Removed1 Publication
Chaini2 – 421420Glutamate dehydrogenasePRO_0000182759Add
BLAST

Interactioni

Subunit structurei

Homohexamer.

Protein-protein interaction databases

STRINGi69014.TK1431.

Structurei

3D structure databases

ProteinModelPortaliO59650.
SMRiO59650. Positions 3-421.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG0334.
HOGENOMiHOG000243801.
InParanoidiO59650.
KOiK00261.

Family and domain databases

Gene3Di3.40.50.720. 1 hit.
InterProiIPR006095. Glu/Leu/Phe/Val_DH.
IPR006096. Glu/Leu/Phe/Val_DH_C.
IPR006097. Glu/Leu/Phe/Val_DH_dimer_dom.
IPR014362. Glu_DH.
IPR016040. NAD(P)-bd_dom.
[Graphical view]
PfamiPF00208. ELFV_dehydrog. 1 hit.
PF02812. ELFV_dehydrog_N. 1 hit.
[Graphical view]
PIRSFiPIRSF000185. Glu_DH. 1 hit.
PRINTSiPR00082. GLFDHDRGNASE.
SMARTiSM00839. ELFV_dehydrog. 1 hit.
[Graphical view]
PROSITEiPS00074. GLFV_DEHYDROGENASE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

O59650-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MVEIDPFEMA VQQLERAAQF MDISEEALEW LKRPMRIVEV SVPVEMDDGS
60 70 80 90 100
VKVFTGFRVQ HNWARGPTKG GIRWHPAETL STVKALATWM TWKVAVVDLP
110 120 130 140 150
YGGGKGGIIV DPKKLSEREQ ERLARSYIRA VYDVIGPWTD IPAPDVYTNP
160 170 180 190 200
KIMAWMMDEY ETIMRRKGPA FGVITGKPPG VGGIVARMDA TARGAAFTIR
210 220 230 240 250
EAAKALGWDD LKGKTIAIQG YGNAGYYLHK IMSEEFGMKV VAVSDSKGGI
260 270 280 290 300
YNPDGLPPAD EVLKWKKEHG SVKDMPGTQN ITNEELLELE VDILAPSAIE
310 320 330 340 350
GVITKENADN VKAKIVAEVA NGPVTPEADE ILHEKGILQI PDFLCNAGGV
360 370 380 390 400
TVSYFEWVQN INGFYWTVEE TRKRLDDKMT KAFWDVFNTH KEKNIHMRDA
410 420
AYVVAVSRVY EAMKHRGWVK K
Length:421
Mass (Da):47,056
Last modified:January 23, 2007 - v4
Checksum:i13EF3F1DD1602E51
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti138 – 1381W → C in BAA25261. (PubMed:9520268)Curated
Sequence conflicti347 – 3471A → T in BAA25261. (PubMed:9520268)Curated

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
D89911 Genomic DNA. Translation: BAA25261.1.
AP006878 Genomic DNA. Translation: BAD85620.1.
PIRiT44789.
RefSeqiYP_183844.1. NC_006624.1.

Genome annotation databases

EnsemblBacteriaiBAD85620; BAD85620; TK1431.
GeneIDi3234478.
KEGGitko:TK1431.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
D89911 Genomic DNA. Translation: BAA25261.1 .
AP006878 Genomic DNA. Translation: BAD85620.1 .
PIRi T44789.
RefSeqi YP_183844.1. NC_006624.1.

3D structure databases

ProteinModelPortali O59650.
SMRi O59650. Positions 3-421.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 69014.TK1431.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai BAD85620 ; BAD85620 ; TK1431 .
GeneIDi 3234478.
KEGGi tko:TK1431.

Phylogenomic databases

eggNOGi COG0334.
HOGENOMi HOG000243801.
InParanoidi O59650.
KOi K00261.

Enzyme and pathway databases

BioCyci TKOD69014:GH72-1456-MONOMER.
SABIO-RK O59650.

Family and domain databases

Gene3Di 3.40.50.720. 1 hit.
InterProi IPR006095. Glu/Leu/Phe/Val_DH.
IPR006096. Glu/Leu/Phe/Val_DH_C.
IPR006097. Glu/Leu/Phe/Val_DH_dimer_dom.
IPR014362. Glu_DH.
IPR016040. NAD(P)-bd_dom.
[Graphical view ]
Pfami PF00208. ELFV_dehydrog. 1 hit.
PF02812. ELFV_dehydrog_N. 1 hit.
[Graphical view ]
PIRSFi PIRSF000185. Glu_DH. 1 hit.
PRINTSi PR00082. GLFDHDRGNASE.
SMARTi SM00839. ELFV_dehydrog. 1 hit.
[Graphical view ]
PROSITEi PS00074. GLFV_DEHYDROGENASE. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Sequence analysis of glutamate dehydrogenase (GDH) from the hyperthermophilic archaeon Pyrococcus sp. KOD1 and comparison of the enzymatic characteristics of native and recombinant GDHs."
    Rahman R.N.Z.A., Fujiwara S., Takagi M., Imanaka T.
    Mol. Gen. Genet. 257:338-347(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 2-11.
    Strain: ATCC BAA-918 / JCM 12380 / KOD1.
  2. "Complete genome sequence of the hyperthermophilic archaeon Thermococcus kodakaraensis KOD1 and comparison with Pyrococcus genomes."
    Fukui T., Atomi H., Kanai T., Matsumi R., Fujiwara S., Imanaka T.
    Genome Res. 15:352-363(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC BAA-918 / JCM 12380 / KOD1.

Entry informationi

Entry nameiDHE3_THEKO
AccessioniPrimary (citable) accession number: O59650
Secondary accession number(s): Q5JDK3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 15, 1998
Last sequence update: January 23, 2007
Last modified: October 29, 2014
This is version 94 of the entry and version 4 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Miscellaneous

Uses both NAD and NADP.

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3