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O59650 (DHE3_THEKO) Reviewed, UniProtKB/Swiss-Prot

Last modified May 14, 2014. Version 92. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Glutamate dehydrogenase

Short name=GDH
EC=1.4.1.3
Gene names
Name:gdhA
Ordered Locus Names:TK1431
OrganismThermococcus kodakaraensis (strain ATCC BAA-918 / JCM 12380 / KOD1) (Pyrococcus kodakaraensis (strain KOD1)) [Reference proteome] [HAMAP]
Taxonomic identifier69014 [NCBI]
Taxonomic lineageArchaeaEuryarchaeotaThermococciThermococcalesThermococcaceaeThermococcus

Protein attributes

Sequence length421 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Catalytic activity

L-glutamate + H2O + NAD(P)+ = 2-oxoglutarate + NH3 + NAD(P)H.

Subunit structure

Homohexamer.

Subcellular location

Cytoplasm.

Miscellaneous

Uses both NAD and NADP.

Sequence similarities

Belongs to the Glu/Leu/Phe/Val dehydrogenases family.

Ontologies

Keywords
   Cellular componentCytoplasm
   LigandNAD
NADP
   Molecular functionOxidoreductase
   Technical termComplete proteome
Direct protein sequencing
Reference proteome
Gene Ontology (GO)
   Biological_processcellular amino acid metabolic process

Inferred from electronic annotation. Source: InterPro

   Cellular_componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionglutamate dehydrogenase [NAD(P)+] activity

Inferred from electronic annotation. Source: UniProtKB-EC

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Initiator methionine11Removed Ref.1
Chain2 – 421420Glutamate dehydrogenase
PRO_0000182759

Regions

Nucleotide binding220 – 2267NAD Potential

Sites

Active site1051 By similarity

Experimental info

Sequence conflict1381W → C in BAA25261. Ref.1
Sequence conflict3471A → T in BAA25261. Ref.1

Sequences

Sequence LengthMass (Da)Tools
O59650 [UniParc].

Last modified January 23, 2007. Version 4.
Checksum: 13EF3F1DD1602E51

FASTA42147,056
        10         20         30         40         50         60 
MVEIDPFEMA VQQLERAAQF MDISEEALEW LKRPMRIVEV SVPVEMDDGS VKVFTGFRVQ 

        70         80         90        100        110        120 
HNWARGPTKG GIRWHPAETL STVKALATWM TWKVAVVDLP YGGGKGGIIV DPKKLSEREQ 

       130        140        150        160        170        180 
ERLARSYIRA VYDVIGPWTD IPAPDVYTNP KIMAWMMDEY ETIMRRKGPA FGVITGKPPG 

       190        200        210        220        230        240 
VGGIVARMDA TARGAAFTIR EAAKALGWDD LKGKTIAIQG YGNAGYYLHK IMSEEFGMKV 

       250        260        270        280        290        300 
VAVSDSKGGI YNPDGLPPAD EVLKWKKEHG SVKDMPGTQN ITNEELLELE VDILAPSAIE 

       310        320        330        340        350        360 
GVITKENADN VKAKIVAEVA NGPVTPEADE ILHEKGILQI PDFLCNAGGV TVSYFEWVQN 

       370        380        390        400        410        420 
INGFYWTVEE TRKRLDDKMT KAFWDVFNTH KEKNIHMRDA AYVVAVSRVY EAMKHRGWVK 


K 

« Hide

References

« Hide 'large scale' references
[1]"Sequence analysis of glutamate dehydrogenase (GDH) from the hyperthermophilic archaeon Pyrococcus sp. KOD1 and comparison of the enzymatic characteristics of native and recombinant GDHs."
Rahman R.N.Z.A., Fujiwara S., Takagi M., Imanaka T.
Mol. Gen. Genet. 257:338-347(1998) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 2-11.
Strain: ATCC BAA-918 / JCM 12380 / KOD1.
[2]"Complete genome sequence of the hyperthermophilic archaeon Thermococcus kodakaraensis KOD1 and comparison with Pyrococcus genomes."
Fukui T., Atomi H., Kanai T., Matsumi R., Fujiwara S., Imanaka T.
Genome Res. 15:352-363(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC BAA-918 / JCM 12380 / KOD1.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
D89911 Genomic DNA. Translation: BAA25261.1.
AP006878 Genomic DNA. Translation: BAD85620.1.
PIRT44789.
RefSeqYP_183844.1. NC_006624.1.

3D structure databases

ProteinModelPortalO59650.
SMRO59650. Positions 3-421.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING69014.TK1431.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaBAD85620; BAD85620; TK1431.
GeneID3234478.
KEGGtko:TK1431.

Phylogenomic databases

eggNOGCOG0334.
HOGENOMHOG000243801.
KOK00261.

Enzyme and pathway databases

BioCycTKOD69014:GH72-1456-MONOMER.
SABIO-RKO59650.

Family and domain databases

Gene3D3.40.50.720. 1 hit.
InterProIPR006095. Glu/Leu/Phe/Val_DH.
IPR006096. Glu/Leu/Phe/Val_DH_C.
IPR006097. Glu/Leu/Phe/Val_DH_dimer_dom.
IPR014362. Glu_DH.
IPR016040. NAD(P)-bd_dom.
[Graphical view]
PfamPF00208. ELFV_dehydrog. 1 hit.
PF02812. ELFV_dehydrog_N. 1 hit.
[Graphical view]
PIRSFPIRSF000185. Glu_DH. 1 hit.
PRINTSPR00082. GLFDHDRGNASE.
SMARTSM00839. ELFV_dehydrog. 1 hit.
[Graphical view]
PROSITEPS00074. GLFV_DEHYDROGENASE. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameDHE3_THEKO
AccessionPrimary (citable) accession number: O59650
Secondary accession number(s): Q5JDK3
Entry history
Integrated into UniProtKB/Swiss-Prot: December 15, 1998
Last sequence update: January 23, 2007
Last modified: May 14, 2014
This is version 92 of the entry and version 4 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families