Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Glutamine synthetase

Gene

glnA

Organism
Methanococcus maripaludis (strain S2 / LL)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Probably involved in nitrogen metabolism via ammonium assimilation. Catalyzes the ATP-dependent biosynthesis of glutamine from glutamate and ammonia.By similarity

Catalytic activityi

ATP + L-glutamate + NH3 = ADP + phosphate + L-glutamine.By similarity

Cofactori

Mg2+By similarityNote: Binds 2 Mg2+ ions per subunit.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi137Magnesium 1By similarity1
Metal bindingi139Magnesium 2By similarity1
Binding sitei187ATPBy similarity1
Metal bindingi192Magnesium 2By similarity1
Metal bindingi199Magnesium 2By similarity1
Binding sitei244L-glutamate; via carbonyl oxygenBy similarity1
Metal bindingi248Magnesium 1; via pros nitrogenBy similarity1
Binding sitei252ATPBy similarity1
Binding sitei301L-glutamateBy similarity1
Binding sitei307L-glutamateBy similarity1
Binding sitei319ATPBy similarity1
Binding sitei319L-glutamateBy similarity1
Binding sitei324ATPBy similarity1
Binding sitei331ATPBy similarity1
Metal bindingi336Magnesium 1By similarity1
Binding sitei338L-glutamateBy similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi250 – 252ATPBy similarity3

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionLigase
LigandATP-binding, Magnesium, Metal-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Glutamine synthetaseBy similarity (EC:6.3.1.2By similarity)
Short name:
GSBy similarity
Alternative name(s):
Glutamate--ammonia ligaseBy similarity
Glutamine synthetase I alphaBy similarity
Short name:
GSI alphaBy similarity
Gene namesi
Name:glnABy similarity
Ordered Locus Names:MMP1206
OrganismiMethanococcus maripaludis (strain S2 / LL)
Taxonomic identifieri267377 [NCBI]
Taxonomic lineageiArchaeaEuryarchaeotaMethanococciMethanococcalesMethanococcaceaeMethanococcus
Proteomesi
  • UP000000590 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm By similarity

GO - Cellular componenti

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001532051 – 446Glutamine synthetaseAdd BLAST446

Interactioni

Subunit structurei

Oligomer of 12 subunits arranged in the form of two hexagons.By similarity

Protein-protein interaction databases

STRINGi267377.MMP1206.

Structurei

3D structure databases

ProteinModelPortaliO59648.
SMRiO59648.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni243 – 244L-glutamate bindingBy similarity2

Sequence similaritiesi

Belongs to the glutamine synthetase family.By similarity

Phylogenomic databases

eggNOGiarCOG01909. Archaea.
COG0174. LUCA.
HOGENOMiHOG000005156.
KOiK01915.
OMAiIEAAWNT.
OrthoDBiPOG093Z00GE.

Family and domain databases

Gene3Di3.10.20.70. 1 hit.
3.30.590.10. 1 hit.
InterProiView protein in InterPro
IPR008147. Gln_synt_b-grasp.
IPR014746. Gln_synth/guanido_kin_cat_dom.
IPR008146. Gln_synth_cat_dom.
IPR027303. Gln_synth_gly_rich_site.
IPR004809. Gln_synth_I.
IPR027302. Gln_synth_N_conserv_site.
PfamiView protein in Pfam
PF00120. Gln-synt_C. 1 hit.
PF03951. Gln-synt_N. 1 hit.
SMARTiView protein in SMART
SM01230. Gln-synt_C. 1 hit.
SUPFAMiSSF54368. SSF54368. 1 hit.
TIGRFAMsiTIGR00653. GlnA. 1 hit.
PROSITEiView protein in PROSITE
PS00180. GLNA_1. 1 hit.
PS00181. GLNA_ATP. 1 hit.

Sequencei

Sequence statusi: Complete.

O59648-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MNAVEQAMEY IKTNNVKFIR FQFVDIHGEP KNIAYPVKAG AAGEEELYDV
60 70 80 90 100
LEKGVYFDGS SIEGFVSIES SDMMLKPDLK TLSVLPWRPT EKSVARVICD
110 120 130 140 150
VYTTNGKPFE GDPRGCLKRV LAKFDEELGG EFFVGPEPEF FILKEDACGS
160 170 180 190 200
WVPADDAGYF DLEPLDGGCD IRRKIVFALE NLGFHVEASH HEVAEGQHEV
210 220 230 240 250
DFKFADAVKT ADSVVTFKTT IKTLAAQDGL KATFMPKPFF GINGSGMHCH
260 270 280 290 300
QSIWLNGEPS FYDESAQYQL SETCMSYVAG ILDHAKSIVA VTNPTVNSYK
310 320 330 340 350
RLVPGYEAPV NIAWANSNRS AIVRVPAPRG KGTRIEFRAP DPACNPYLAF
360 370 380 390 400
TVMLAAGLDG VKRKLSAIEP VEKNIFAMSE AQKKAEGIES VPANLKAALD
410 420 430 440
ELENNSVLKD ALGKHIFENF IEIKNAEWDS FRTAVTDWET KQYLKI
Length:446
Mass (Da):49,421
Last modified:August 1, 1998 - v1
Checksum:iDDE984106651122A
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF062391 Genomic DNA. Translation: AAD04845.1.
BX950229 Genomic DNA. Translation: CAF30762.1.
RefSeqiWP_011171150.1. NC_005791.1.

Genome annotation databases

EnsemblBacteriaiCAF30762; CAF30762; MMP1206.
GeneIDi2761968.
KEGGimmp:MMP1206.
PATRICifig|267377.15.peg.1239.

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.

Entry informationi

Entry nameiGLN1A_METMP
AccessioniPrimary (citable) accession number: O59648
Entry historyiIntegrated into UniProtKB/Swiss-Prot: December 15, 1998
Last sequence update: August 1, 1998
Last modified: June 7, 2017
This is version 88 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families