Reviewed, UniProtKB/Swiss-Prot O59647 (MTMW_METWO)

Names and origin

Protein names

Recommended name:
    Modification methylase MwoI
      Short name=M.MwoI
    EC=2.1.1.113
Alternative name(s):
    N-4 cytosine-specific methyltransferase MwoI

Gene names

Name:

mwoIM

Organism

Methanobacterium wolfei

Taxonomic identifier

145261 [NCBI]

Taxonomic lineage

Archaea › Euryarchaeota › Methanobacteria › Methanobacteriales › Methanobacteriaceae › Methanothermobacter

Protein attributes

Sequence length	668 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Recognizes the double-stranded DNA sequence GCNNNNNNNGC, causes specific methylation on N-4 on both strands, and protects the DNA from cleavage by the MwoI endonuclease.
Catalytic activity	S-adenosyl-L-methionine + DNA cytosine = S-adenosyl-L-homocysteine + DNA N(4)-methylcytosine.
Sequence similarities	Belongs to the N(4)/N(6)-methyltransferase family. N(4) subfamily.

Ontologies

Keywords
Biological process	Restriction system
Ligand	S-adenosyl-L-methionine
Molecular function	Methyltransferase Transferase
Gene Ontology (GO)
Biological process	DNA methylation on cytosine Inferred from electronic annotation. Source: InterPro DNA restriction-modification system Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	DNA binding Inferred from electronic annotation. Source: InterPro N-methyltransferase activity Inferred from electronic annotation. Source: InterPro site-specific DNA-methyltransferase (cytosine-N4-specific) activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 668	668	Modification methylase MwoI		PRO_0000087930

Sequences

	Sequence		Length	Mass (Da)
	O59647-1 [UniParc]. Last modified August 1, 1998. Version 1. Checksum: 7F5B53A2807912D3	FASTA	668	77,723
	10 20 30 40 50 60 MVKGKILFGD VFSALRCLED NSISVALTSP PYWRQRDYGF KGQIGREKTP EEYIGRLIVI 70 80 90 100 110 120 FRELRAKLKD DGVFFLNIGD KYKNRYGKSH LLQIPYRLAA HMIKDGWKLL DIIIWYKPNH 130 140 150 160 170 180 MPSSVKDRFT NTYEPVLVFG KSDENIYTKK HPVLKIPLQQ TKWKHTAVFP EKLVSSLLSR 190 200 210 220 230 240 CNLKDGDYIL DPFAGTGTTG AVVKKMKYQL YPKDLNVILI EKGKKFLDII TERTGIKEIK 250 260 270 280 290 300 ELKSSEYTWE PVNDKLAFSE DKPLIIIEDT HGETFIAKNS EEFSRIIMGM LSEEFQDFHR 310 320 330 340 350 360 EDAVYFFGVK NWKLSDLVLP GLLIDHGFIL RNMIIIEDGS SWYPVFMLVK DTTRVNYKFY 370 380 390 400 410 420 IDRIRKKPKT VLPEKWNQED FIGLIVNDNL SKKPRKGEVV DIISTYSQDN FPKIVAVSWE 430 440 450 460 470 480 DDNISLELCL NPRKDEFIME SLQFTCPHCG TQLIDTYDPL GDNICYNCQK EIYGKNSLPI 490 500 510 520 530 540 LKESKEIIES LESVENGEYQ VGENIKPQYQ KRCKESKSKF AGMERMNWGA SPGARKTIIG 550 560 570 580 590 600 DSFSKMRLYR LDQPTIARYL NIYMKKNDLR IKDITQALPP EYKHTVGHWF RKDFGGSIPL 610 620 630 640 650 660 PEDVTLLEEI LKLDKEFARI LKRSVLKLQT VKHSLKGKNP GDFLELEENK LKEYLTKTYM PPSYYIKK

References

[1]	"Method for producing the MwoI restriction endonuclease and methylase." Lunnen K.D., Wilson G.G. Patent number US5053330, 01-OCT-1991 Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: DSM 2970.
[2]	"Characterization and cloning of MwoI (GCN7GC), a new type-II restriction-modification system from Methanobacterium wolfei." Lunnen K.D., Morgan R.D., Timan C.J., Krzycki J.A., Reeve J.N., Wilson G.G. Gene 77:11-19(1989) [PubMed: 2663652] [Abstract] Cited for: CHARACTERIZATION. Strain: DSM 2970.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AF051376 Genomic DNA. Translation: AAC05700.1.
3D structure databases
ModBase	Search...
Protein family/group databases
REBASE	3451. M.MwoI.
Enzyme and pathway databases
BRENDA	2.1.1.113. 7575.
Family and domain databases
InterPro	IPR002941. DNA_methylase_N4/N6. IPR001091. MeTrfase_CN4. IPR017985. MeTrfase_CN4_CS. [Graphical view]
Pfam	PF01555. N6_N4_Mtase. 1 hit. [Graphical view]
PRINTS	PR00508. S21N4MTFRASE.
PROSITE	PS00093. N4_MTASE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

MTMW_METWO

Accession

Primary (citable) accession number: O59647

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 15, 1998
Last sequence update:	August 1, 1998
Last modified:	June 16, 2009
This is version 48 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)