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O59646 (T2MW_METWO) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 41. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Type-2 restriction enzyme MwoI

Short name=R.MwoI
EC=3.1.21.4
Alternative name(s):
Endonuclease MwoI
Type II restriction enzyme MwoI
Gene names
Name:mwoIR
OrganismMethanothermobacter wolfeii (Methanobacterium wolfei)
Taxonomic identifier145261 [NCBI]
Taxonomic lineageArchaeaEuryarchaeotaMethanobacteriaMethanobacterialesMethanobacteriaceaeMethanothermobacter

Protein attributes

Sequence length363 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Recognizes the double-stranded sequence GCNNNNNNNGC and cleaves after N-7.

Catalytic activity

Endonucleolytic cleavage of DNA to give specific double-stranded fragments with terminal 5'-phosphates.

Ontologies

Keywords
   Biological processRestriction system
   Molecular functionEndonuclease
Hydrolase
Nuclease
Gene Ontology (GO)
   Biological_processDNA restriction-modification system

Inferred from electronic annotation. Source: UniProtKB-KW

   Molecular_functionType II site-specific deoxyribonuclease activity

Inferred from electronic annotation. Source: UniProtKB-EC

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 363363Type-2 restriction enzyme MwoI
PRO_0000077342

Sequences

Sequence LengthMass (Da)Tools
O59646 [UniParc].

Last modified August 1, 1998. Version 1.
Checksum: 5CC35411A18028A4

FASTA36342,214
        10         20         30         40         50         60 
MKNPKFTENQ KEIEKEEFEF LQKLNFIIKE SLELFNTNLK NSMKFINYIT LPIIMASIES 

        70         80         90        100        110        120 
KSFNPFSEII EKHIAFILNS KMNSLGYKFL PLGYSSDLTY ENDNSIIHID IKTANLENPS 

       130        140        150        160        170        180 
DFKDTVPLGI NQSSYPGVLD CKIRGKNIKA DCKKIKVYPN IPTTYNNKLT ITNALLFIYP 

       190        200        210        220        230        240 
DYKEIIDEIR EDYIAIRELI SINLKDILTP IEGSLEEFLN YKPSNEKKRL EPILDNIVRG 

       250        260        270        280        290        300 
YFIHDKLRHE FSENVEKDLE EFEKKIIGIA KKLKEREIKP VAILSISIPN GELAPHYDDE 

       310        320        330        340        350        360 
IVSGKSWGSS FRYHYKKSGN SVFKGLDNKA SRAVFLHINK EYLPVLKKYF DPITVYELTE 


KRL 

« Hide

References

[1]"Method for producing the MwoI restriction endonuclease and methylase."
Lunnen K.D., Wilson G.G.
Patent number US5053330, 01-OCT-1991
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: ATCC 43096 / DSM 2970 / JCM 14652 / NBRC 100332 / VKM B-1829.
[2]"Characterization and cloning of MwoI (GCN7GC), a new type-II restriction-modification system from Methanobacterium wolfei."
Lunnen K.D., Morgan R.D., Timan C.J., Krzycki J.A., Reeve J.N., Wilson G.G.
Gene 77:11-19(1989) [PubMed] [Europe PMC] [Abstract]
Cited for: CHARACTERIZATION.
Strain: ATCC 43096 / DSM 2970 / JCM 14652 / NBRC 100332 / VKM B-1829.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AF051376 Genomic DNA. Translation: AAC05699.1.

3D structure databases

ModBaseSearch...
MobiDBSearch...

Protein family/group databases

REBASE1291. MwoI.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Family and domain databases

ProtoNetSearch...

Entry information

Entry nameT2MW_METWO
AccessionPrimary (citable) accession number: O59646
Entry history
Integrated into UniProtKB/Swiss-Prot: December 15, 1998
Last sequence update: August 1, 1998
Last modified: April 16, 2014
This is version 41 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

Restriction enzymes and methylases

Classification of restriction enzymes and methylases and list of entries