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Protein

Type-2 restriction enzyme MwoI

Gene

mwoIR

Organism
Methanothermobacter wolfeii (Methanobacterium wolfei)
Status
Reviewed-Annotation score: Annotation score: 2 out of 5-Experimental evidence at protein leveli

Functioni

Recognizes the double-stranded sequence GCNNNNNNNGC and cleaves after N-7.

Catalytic activityi

Endonucleolytic cleavage of DNA to give specific double-stranded fragments with terminal 5'-phosphates.

GO - Molecular functioni

  1. Type II site-specific deoxyribonuclease activity Source: UniProtKB-EC

GO - Biological processi

  1. DNA restriction-modification system Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Endonuclease, Hydrolase, Nuclease

Keywords - Biological processi

Restriction system

Protein family/group databases

REBASEi1291. MwoI.

Names & Taxonomyi

Protein namesi
Recommended name:
Type-2 restriction enzyme MwoI (EC:3.1.21.4)
Short name:
R.MwoI
Alternative name(s):
Endonuclease MwoI
Type II restriction enzyme MwoI
Gene namesi
Name:mwoIR
OrganismiMethanothermobacter wolfeii (Methanobacterium wolfei)
Taxonomic identifieri145261 [NCBI]
Taxonomic lineageiArchaeaEuryarchaeotaMethanobacteriaMethanobacterialesMethanobacteriaceaeMethanothermobacter

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 363363Type-2 restriction enzyme MwoIPRO_0000077342Add
BLAST

Sequencei

Sequence statusi: Complete.

O59646-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKNPKFTENQ KEIEKEEFEF LQKLNFIIKE SLELFNTNLK NSMKFINYIT
60 70 80 90 100
LPIIMASIES KSFNPFSEII EKHIAFILNS KMNSLGYKFL PLGYSSDLTY
110 120 130 140 150
ENDNSIIHID IKTANLENPS DFKDTVPLGI NQSSYPGVLD CKIRGKNIKA
160 170 180 190 200
DCKKIKVYPN IPTTYNNKLT ITNALLFIYP DYKEIIDEIR EDYIAIRELI
210 220 230 240 250
SINLKDILTP IEGSLEEFLN YKPSNEKKRL EPILDNIVRG YFIHDKLRHE
260 270 280 290 300
FSENVEKDLE EFEKKIIGIA KKLKEREIKP VAILSISIPN GELAPHYDDE
310 320 330 340 350
IVSGKSWGSS FRYHYKKSGN SVFKGLDNKA SRAVFLHINK EYLPVLKKYF
360
DPITVYELTE KRL
Length:363
Mass (Da):42,214
Last modified:July 31, 1998 - v1
Checksum:i5CC35411A18028A4
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF051376 Genomic DNA. Translation: AAC05699.1.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF051376 Genomic DNA. Translation: AAC05699.1.

3D structure databases

ModBaseiSearch...
MobiDBiSearch...

Protein family/group databases

REBASEi1291. MwoI.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Family and domain databases

ProtoNetiSearch...

Publicationsi

  1. "Method for producing the MwoI restriction endonuclease and methylase."
    Lunnen K.D., Wilson G.G.
    Patent number US5053330, 30-SEP-1991
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ATCC 43096 / DSM 2970 / JCM 14652 / NBRC 100332 / VKM B-1829.
  2. "Characterization and cloning of MwoI (GCN7GC), a new type-II restriction-modification system from Methanobacterium wolfei."
    Lunnen K.D., Morgan R.D., Timan C.J., Krzycki J.A., Reeve J.N., Wilson G.G.
    Gene 77:11-19(1988) [PubMed] [Europe PMC] [Abstract]
    Cited for: CHARACTERIZATION.
    Strain: ATCC 43096 / DSM 2970 / JCM 14652 / NBRC 100332 / VKM B-1829.

Entry informationi

Entry nameiT2MW_METWO
AccessioniPrimary (citable) accession number: O59646
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 14, 1998
Last sequence update: July 31, 1998
Last modified: April 15, 2014
This is version 41 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Documents

  1. Restriction enzymes and methylases
    Classification of restriction enzymes and methylases and list of entries

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.