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O59646

- T2MW_METWO

UniProt

O59646 - T2MW_METWO

Protein

Type-2 restriction enzyme MwoI

Gene

mwoIR

Organism
Methanothermobacter wolfeii (Methanobacterium wolfei)
Status
Reviewed - Annotation score: 2 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 41 (16 Apr 2014)
      Sequence version 1 (01 Aug 1998)
      Previous versions | rss
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    Functioni

    Recognizes the double-stranded sequence GCNNNNNNNGC and cleaves after N-7.

    Catalytic activityi

    Endonucleolytic cleavage of DNA to give specific double-stranded fragments with terminal 5'-phosphates.

    GO - Molecular functioni

    1. Type II site-specific deoxyribonuclease activity Source: UniProtKB-EC

    GO - Biological processi

    1. DNA restriction-modification system Source: UniProtKB-KW

    Keywords - Molecular functioni

    Endonuclease, Hydrolase, Nuclease

    Keywords - Biological processi

    Restriction system

    Protein family/group databases

    REBASEi1291. MwoI.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Type-2 restriction enzyme MwoI (EC:3.1.21.4)
    Short name:
    R.MwoI
    Alternative name(s):
    Endonuclease MwoI
    Type II restriction enzyme MwoI
    Gene namesi
    Name:mwoIR
    OrganismiMethanothermobacter wolfeii (Methanobacterium wolfei)
    Taxonomic identifieri145261 [NCBI]
    Taxonomic lineageiArchaeaEuryarchaeotaMethanobacteriaMethanobacterialesMethanobacteriaceaeMethanothermobacter

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 363363Type-2 restriction enzyme MwoIPRO_0000077342Add
    BLAST

    Sequencei

    Sequence statusi: Complete.

    O59646-1 [UniParc]FASTAAdd to Basket

    « Hide

    MKNPKFTENQ KEIEKEEFEF LQKLNFIIKE SLELFNTNLK NSMKFINYIT    50
    LPIIMASIES KSFNPFSEII EKHIAFILNS KMNSLGYKFL PLGYSSDLTY 100
    ENDNSIIHID IKTANLENPS DFKDTVPLGI NQSSYPGVLD CKIRGKNIKA 150
    DCKKIKVYPN IPTTYNNKLT ITNALLFIYP DYKEIIDEIR EDYIAIRELI 200
    SINLKDILTP IEGSLEEFLN YKPSNEKKRL EPILDNIVRG YFIHDKLRHE 250
    FSENVEKDLE EFEKKIIGIA KKLKEREIKP VAILSISIPN GELAPHYDDE 300
    IVSGKSWGSS FRYHYKKSGN SVFKGLDNKA SRAVFLHINK EYLPVLKKYF 350
    DPITVYELTE KRL 363
    Length:363
    Mass (Da):42,214
    Last modified:August 1, 1998 - v1
    Checksum:i5CC35411A18028A4
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF051376 Genomic DNA. Translation: AAC05699.1.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF051376 Genomic DNA. Translation: AAC05699.1 .

    3D structure databases

    ModBasei Search...
    MobiDBi Search...

    Protein family/group databases

    REBASEi 1291. MwoI.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Family and domain databases

    ProtoNeti Search...

    Publicationsi

    1. "Method for producing the MwoI restriction endonuclease and methylase."
      Lunnen K.D., Wilson G.G.
      Patent number US5053330, 01-OCT-1991
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: ATCC 43096 / DSM 2970 / JCM 14652 / NBRC 100332 / VKM B-1829.
    2. "Characterization and cloning of MwoI (GCN7GC), a new type-II restriction-modification system from Methanobacterium wolfei."
      Lunnen K.D., Morgan R.D., Timan C.J., Krzycki J.A., Reeve J.N., Wilson G.G.
      Gene 77:11-19(1989) [PubMed] [Europe PMC] [Abstract]
      Cited for: CHARACTERIZATION.
      Strain: ATCC 43096 / DSM 2970 / JCM 14652 / NBRC 100332 / VKM B-1829.

    Entry informationi

    Entry nameiT2MW_METWO
    AccessioniPrimary (citable) accession number: O59646
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: December 15, 1998
    Last sequence update: August 1, 1998
    Last modified: April 16, 2014
    This is version 41 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Documents

    1. Restriction enzymes and methylases
      Classification of restriction enzymes and methylases and list of entries

    External Data

    Dasty 3