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Reviewed, UniProtKB/Swiss-Prot O59645 (AGLU_SULSO)

Last modified June 16, 2009. Version 59. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data | Customize display text xml rdf/xml gff fasta
Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

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Names and origin

Protein namesRecommended name:
    Alpha-glucosidase
    EC=3.2.1.20
Alternative name(s):
    Maltase
Gene names
Name: malA
Ordered Locus Names: SSO3051
ORF Names: C23_036
OrganismSulfolobus solfataricus [Complete proteome] [HAMAP]
Taxonomic identifier2287 [NCBI]
Taxonomic lineageArchaea › Crenarchaeota › Thermoprotei › Sulfolobales › Sulfolobaceae › Sulfolobus

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Protein attributes

Sequence length693 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is not processed.
Protein existenceEvidence at protein level.

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General annotation (Comments)

Function

Major soluble alpha-glucosidase.

Catalytic activity

Hydrolysis of terminal, non-reducing (1->4)-linked alpha-D-glucose residues with release of alpha-D-glucose.

Subcellular location

Cytoplasm.

Induction

Expressed during growth on maltose.

Sequence similarities

Belongs to the glycosyl hydrolase 31 family.

biophysicochemical properties

pH dependence:

Optimum pH is 4.5 for maltose hydrolysis, and 5.5 for glucogen hydrolysis.

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Ontologies

Keywords
   Cellular componentCytoplasm
   Molecular functionGlycosidase
Hydrolase
   Technical term3D-structure
Complete proteome
Direct protein sequencing
Gene Ontology (GO)
   Biological processcarbohydrate metabolic process

Inferred from electronic annotation. Source: InterPro

   Cellular componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular functionalpha-glucosidase activity

Inferred from electronic annotation. Source: EC

Complete GO annotation...

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Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 693693Alpha-glucosidase
PRO_0000185367

Sites

Active site3201 By similarity
Active site3231 By similarity
Active site4161Proton donor By similarity

Secondary structure

1 ................................................................................................................................. 693
Helix Strand Turn

Details...

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Sequences

Sequence LengthMass (Da)Tools
O59645-1 [UniParc].

Last modified August 1, 1998. Version 1.
Checksum: 27BB952C0A7B3858

FASTA69380,441
        10         20         30         40         50         60 
MQTIKIYENK GVYKVVIGEP FPPIEFPLEQ KISSNKSLSE LGLTIVQQGN KVIVEKSLDL 

        70         80         90        100        110        120 
KEHIIGLGEK AFELDRKRKR YVMYNVDAGA YKKYQDPLYV SIPLFISVKD GVATGYFFNS 

       130        140        150        160        170        180 
ASKVIFDVGL EEYDKVIVTI PEDSVEFYVI EGPRIEDVLE KYTELTGKPF LPPMWAFGYM 

       190        200        210        220        230        240 
ISRYSYYPQD KVVELVDIMQ KEGFRVAGVF LDIHYMDSYK LFTWHPYRFP EPKKLIDELH 

       250        260        270        280        290        300 
KRNVKLITIV DHGIRVDQNY SPFLSGMGKF CEIESGELFV GKMWPGTTVY PDFFREDTRE 

       310        320        330        340        350        360 
WWAGLISEWL SQGVDGIWLD MNEPTDFSRA IEIRDVLSSL PVQFRDDRLV TTFPDNVVHY 

       370        380        390        400        410        420 
LRGKRVKHEK VRNAYPLYEA MATFKGFRTS HRNEIFILSR AGYAGIQRYA FIWTGDNTPS 

       430        440        450        460        470        480 
WDDLKLQLQL VLGLSISGVP FVGCDIGGFQ GRNFAEIDNS MDLLVKYYAL ALFFPFYRSH 

       490        500        510        520        530        540 
KATDGIDTEP VFLPDYYKEK VKEIVELRYK FLPYIYSLAL EASEKGHPVI RPLFYEFQDD 

       550        560        570        580        590        600 
DDMYRIEDEY MVGKYLLYAP IVSKEESRLV TLPRGKWYNY WNGEIINGKS VVKSTHELPI 

       610        620        630        640        650        660 
YLREGSIIPL EGDELIVYGE TSFKRYDNAE ITSSSNEIKF SREIYVSKLT ITSEKPVSKI 

       670        680        690 
IVDDSKEIQV EKTMQNTYVA KINQKIRGKI NLE

« Hide

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References

« Hide 'large scale' references
[1]"Molecular characterization of the alpha-glucosidase gene (malA) from the hyperthermophilic archaeon Sulfolobus solfataricus."
Rolfsmeier M., Haseltine C., Bini E., Clark A., Blum P.
J. Bacteriol. 180:1287-1295(1998) [PubMed: 9495770] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 1-20 AND 552-561.
Strain: 98/2.
[2]"The complete genome of the crenarchaeon Sulfolobus solfataricus P2."
She Q., Singh R.K., Confalonieri F., Zivanovic Y., Allard G., Awayez M.J., Chan-Weiher C.C.-Y., Clausen I.G., Curtis B.A., De Moors A., Erauso G., Fletcher C., Gordon P.M.K., Heikamp-de Jong I., Jeffries A.C., Kozera C.J., Medina N., Peng X. expand/collapse author list , Thi-Ngoc H.P., Redder P., Schenk M.E., Theriault C., Tolstrup N., Charlebois R.L., Doolittle W.F., Duguet M., Gaasterland T., Garrett R.A., Ragan M.A., Sensen C.W., Van der Oost J.
Proc. Natl. Acad. Sci. U.S.A. 98:7835-7840(2001) [PubMed: 11427726] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 35092 / DSM 1617 / JCM 11322 / P2.
+Additional computationally mapped references.

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Cross-references

Sequence databases

AF042494 Genomic DNA. Translation: AAC38215.1.
AE006641 Genomic DNA. Translation: AAK43151.1.
PIRH90486.
RefSeqNP_344361.1.

3D structure databases

EntryMethodResolution (Å)ChainPositionsPDBsum
2G3MX-ray2.55A/B/C/D/E/F5-693[»]
2G3NX-ray2.55A/B/C/D/E/F5-693[»]
ModBaseSearch...

Protein family/group databases

CAZyGH31. Glycoside Hydrolase Family 31.

Genome annotation databases

GeneID1453070.
GenomeReviewsGene locus SSO3051 in contig AE006641_GR.
KEGGsso:SSO3051.
NMPDRfig|273057.1.peg.2784.

Organism-specific databases

CMRSearch...

Phylogenomic databases

HOGENOMO59645.
OMAO59645. ELYLRWF.

Enzyme and pathway databases

BioCycSSOL273057:SSO3051-MON.
BRENDA3.2.1.20. 2070.

Family and domain databases

InterProIPR000322. Glyco_hydro_31.
[Graphical view]
PANTHERPTHR22762. Glyco_hydro_31. 1 hit.
PfamPF01055. Glyco_hydro_31. 1 hit.
[Graphical view]
PROSITEPS00129. GLYCOSYL_HYDROL_F31_1. 1 hit.
PS00707. GLYCOSYL_HYDROL_F31_2. False negative.
[Graphical view]
ProtoNetSearch...

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Entry information

Entry nameAGLU_SULSO
AccessionPrimary (citable) accession number: O59645
Entry history
Integrated into UniProtKB/Swiss-Prot: December 15, 1998
Last sequence update: August 1, 1998
Last modified: June 16, 2009
This is version 59 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation projectHAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)

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Relevant documents

Glycosyl hydrolases

Classification of glycosyl hydrolase families and list of entries

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

SIMILARITY comments

Index of protein domains and families

Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents