O59621 (PURL_PYRHO) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Phosphoribosylformylglycinamidine synthase 2
EC=6.3.5.3

Alternative name(s):
Phosphoribosylformylglycinamidine synthase II
Short name=FGAM synthase II

Gene names

Name:	purL
Ordered Locus Names:	PH1953

Organism

Pyrococcus horikoshii (strain ATCC 700860 / DSM 12428 / JCM 9974 / NBRC 100139 / OT-3) [Complete proteome] [HAMAP]

Taxonomic identifier

70601 [NCBI]

Taxonomic lineage

Archaea › Euryarchaeota › Thermococci › Thermococcales › Thermococcaceae › Pyrococcus

Protein attributes

Sequence length	705 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	ATP + N(2)-formyl-N(1)-(5-phospho-D-ribosyl)glycinamide + L-glutamine + H2O = ADP + phosphate + 2-(formamido)-N(1)-(5-phospho-D-ribosyl)acetamidine + L-glutamate. HAMAP MF_00420
Pathway	Purine metabolism; IMP biosynthesis via de novo pathway; 5-amino-1-(5-phospho-D-ribosyl)imidazole from N(2)-formyl-N(1)-(5-phospho-D-ribosyl)glycinamide: step 1/2. HAMAP MF_00420
Subunit structure	Heterodimer of two subunits, PurQ and PurL.
Subcellular location	Cytoplasm By similarity HAMAP MF_00420.
Sequence similarities	Belongs to the FGAMS family.

Ontologies

Keywords
Biological process	Purine biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	'de novo' IMP biosynthetic process Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW phosphoribosylformylglycinamidine synthase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 705	705	Phosphoribosylformylglycinamidine synthase 2 HAMAP MF_00420		PRO_0000100522
Regions
	Nucleotide binding	89 – 100	12	ATP Potential

Sequences

	Sequence		Length	Mass (Da)
	O59621 [UniParc]. Last modified August 1, 1998. Version 1. Checksum: 57550733AAA4DE29	FASTA	705	78,540
	10 20 30 40 50 60 MFPQEEKLIR ERLGREPNDV ERAMLEVMWS EHVSYKSSRK WLKLLPTKNE HVVLGPGEDA 70 80 90 100 110 120 GIIKFDDKTW IVIGIESHNH PSAVEPYGGA ATGVGGIVRD ILCMGARPIA LLDPIRFGPL 130 140 150 160 170 180 EKEKNRYLFE YVVKGISDYG NRIGVPTVGG ETEFDESLDN YTLVNVACVG IMKPEHLVHS 190 200 210 220 230 240 YVTEPGLKLI IVGNRTGRDG IHGVTFASEE LSENAEEEDR SAVQIPDPFT EKLLIEATLE 250 260 270 280 290 300 AVYTGKVRAL KDLGGGGLTC AASEMVGKRG FGAIIYADKV LLREPGMTPL EVMISESQER 310 320 330 340 350 360 MLFAVRPEDV EELARIFEKY ELEWSVVGEV IEEPKFIVYW KGSKVAELPI DLLTEVPTIE 370 380 390 400 410 420 WPMKEYKIEE EVGIPRISLQ EAFEKVWRSP NIVAKRWIWE QYDHEVQGRT VVKPGFDSAV 430 440 450 460 470 480 LKINEEYGIA ITADGNPNHC YLNPYHGAMG LVAEVVRNLV SVGAKPLALV DNLNFASPER 490 500 510 520 530 540 PEVYWSFAET VKGLADAAKA FNLAYVSGNV SFYNEIVDKP IKPTPVVAGV GKVKLSKIPK 550 560 570 580 590 600 GPNKGDVVTL VGETKRELGG SELYRVLGIR KGIAPRVNLE VERKNAESIL RLINASLVSF 610 620 630 640 650 660 VHDLSRGGLL VALAEVAALF NVGMEVTIKT DMNPVEFAFS ESHGRYLVVL PERKLEEARE 670 680 690 700 ITDLKVIGRI IGSNSFSVKI NDEVLLWNLD KLKDVYWNTL YRLMD

References

[1]

"Complete sequence and gene organization of the genome of a hyper-thermophilic archaebacterium, Pyrococcus horikoshii OT3." Kawarabayasi Y., Sawada M., Horikawa H., Haikawa Y., Hino Y., Yamamoto S., Sekine M., Baba S., Kosugi H., Hosoyama A., Nagai Y., Sakai M., Ogura K., Otsuka R., Nakazawa H., Takamiya M., Ohfuku Y., Funahashi T. , Tanaka T., Kudoh Y., Yamazaki J., Kushida N., Oguchi A., Aoki K., Yoshizawa T., Nakamura Y., Robb F.T., Horikoshi K., Masuchi Y., Shizuya H., Kikuchi H. DNA Res. 5:55-76(1998) [PubMed: 9679194] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 700860 / DSM 12428 / JCM 9974 / NBRC 100139 / OT-3.

Cross-references

Sequence databases
EMBL GenBank DDBJ	BA000001 Genomic DNA. Translation: BAA31080.1.
PIR	A71211.
RefSeq	NP_143781.1. NC_000961.1.
3D structure databases
ProteinModelPortal	O59621.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	EBPYRT00000000290; EBPYRP00000000290; EBPYRG00000000290.
GeneID	1442800.
GenomeReviews	Gene locus PH1953 in contig BA000001_GR.
KEGG	pho:PH1953.
NMPDR	fig|70601.1.peg.1914.
Organism-specific databases
CMR	Search...
Phylogenomic databases
GeneTree	EBGT00050000022555.
HOGENOM	HBG311214.
OMA	YGNSFGV.
PhylomeDB	O59621.
ProtClustDB	PRK01213.
Enzyme and pathway databases
BioCyc	PHOR70601:PH1953-MONOMER.
Family and domain databases
HAMAP	MF_00420. PurL_2. [Tree]
InterPro	IPR000728. AIR_synth. IPR010918. AIR_synth_C. IPR010074. PRibForGlyAmidine_synth_II. IPR016188. PurM_N-like. [Graphical view]
KO	K01952.
Pfam	PF00586. AIRS. 2 hits. PF02769. AIRS_C. 2 hits. [Graphical view]
SUPFAM	SSF56042. AIR_synth_C. 2 hits. SSF55326. PurM_N-like. 2 hits.
TIGRFAMs	TIGR01736. FGAM_synth_II. 1 hit.
ProtoNet	Search...

Entry information

Entry name

PURL_PYRHO

Accession

Primary (citable) accession number: O59621

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 11, 2001
Last sequence update:	August 1, 1998
Last modified:	January 25, 2012
This is version 85 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program