O59619 (PURQ_PYRHO) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Phosphoribosylformylglycinamidine synthase 1
EC=6.3.5.3

Alternative name(s):
Phosphoribosylformylglycinamidine synthase I
Short name=FGAM synthase I

Gene names

Name:	purQ
Ordered Locus Names:	PH1955

Organism

Pyrococcus horikoshii (strain ATCC 700860 / DSM 12428 / JCM 9974 / NBRC 100139 / OT-3) [Complete proteome] [HAMAP]

Taxonomic identifier

70601 [NCBI]

Taxonomic lineage

Archaea › Euryarchaeota › Thermococci › Thermococcales › Thermococcaceae › Pyrococcus

Protein attributes

Sequence length	223 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	ATP + N(2)-formyl-N(1)-(5-phospho-D-ribosyl)glycinamide + L-glutamine + H2O = ADP + phosphate + 2-(formamido)-N(1)-(5-phospho-D-ribosyl)acetamidine + L-glutamate. HAMAP MF_00421
Pathway	Purine metabolism; IMP biosynthesis via de novo pathway; 5-amino-1-(5-phospho-D-ribosyl)imidazole from N(2)-formyl-N(1)-(5-phospho-D-ribosyl)glycinamide: step 1/2. HAMAP MF_00421
Subunit structure	Heterodimer of two subunits, PurQ and PurL.
Subcellular location	Cytoplasm By similarity HAMAP MF_00421.
Sequence similarities	Contains 1 glutamine amidotransferase type-1 domain.
Sequence caution	The sequence BAA31082.1 differs from that shown. Reason: Erroneous initiation.

Ontologies

Keywords
Biological process	Purine biosynthesis
Cellular component	Cytoplasm
Domain	Glutamine amidotransferase
Ligand	ATP-binding Nucleotide-binding
Molecular function	Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	'de novo' IMP biosynthetic process Inferred from electronic annotation. Source: InterPro cobalamin biosynthetic process Inferred from electronic annotation. Source: InterPro glutamine metabolic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW phosphoribosylformylglycinamidine synthase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 223	223	Phosphoribosylformylglycinamidine synthase 1 HAMAP MF_00421		PRO_0000100617
Regions
	Domain	4 – 223	220	Glutamine amidotransferase type-1
Sites
	Active site	85	1	Nucleophile By similarity
	Active site	196	1	By similarity
	Active site	198	1	By similarity

Sequences

	Sequence		Length	Mass (Da)
	O59619 [UniParc]. Last modified July 11, 2001. Version 2. Checksum: 9F685E2E39A92817	FASTA	223	24,934
	10 20 30 40 50 60 MVKFAVIVFP GTNCDFETVE AIKRAGGEAE RVWYKQSVKD YDGVVIPGGF SYADYLRAGA 70 80 90 100 110 120 IAARQKVMEE IRELAEEGRP ILGICNGFQI LTEANLLPGA LRPNKIPRFL CKWVHLKVVD 130 140 150 160 170 180 VETPFTYLYE EGEVVRMPIA HAEGNYYIDN PSKVRIVFQY SDEKGSITEE ANPNGSVLNI 190 200 210 220 AGVTNKQGNV LGMMPHPERA SDRFLGSEDG LKVFKSIVEW MKK

References

[1]

"Complete sequence and gene organization of the genome of a hyper-thermophilic archaebacterium, Pyrococcus horikoshii OT3." Kawarabayasi Y., Sawada M., Horikawa H., Haikawa Y., Hino Y., Yamamoto S., Sekine M., Baba S., Kosugi H., Hosoyama A., Nagai Y., Sakai M., Ogura K., Otsuka R., Nakazawa H., Takamiya M., Ohfuku Y., Funahashi T. , Tanaka T., Kudoh Y., Yamazaki J., Kushida N., Oguchi A., Aoki K., Yoshizawa T., Nakamura Y., Robb F.T., Horikoshi K., Masuchi Y., Shizuya H., Kikuchi H. DNA Res. 5:55-76(1998) [PubMed: 9679194] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 700860 / DSM 12428 / JCM 9974 / NBRC 100139 / OT-3.

Cross-references

Sequence databases
EMBL GenBank DDBJ	BA000001 Genomic DNA. Translation: BAA31082.1. Different initiation.
PIR	C71211.
RefSeq	NP_143783.1. NC_000961.1.
3D structure databases
ProteinModelPortal	O59619.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	EBPYRT00000000287; EBPYRP00000000287; EBPYRG00000000287.
GeneID	1442802.
GenomeReviews	Gene locus PH1955 in contig BA000001_GR.
KEGG	pho:PH1955.
NMPDR	fig|70601.1.peg.1916.
Organism-specific databases
CMR	Search...
Phylogenomic databases
GeneTree	EBGT00050000022620.
HOGENOM	HBG302712.
OMA	FPGTNCD.
PhylomeDB	O59619.
ProtClustDB	PRK03619.
Enzyme and pathway databases
BioCyc	PHOR70601:PH1955-MONOMER.
Family and domain databases
HAMAP	MF_00421. PurQ. [Tree]
InterPro	IPR017926. GATASE_1. IPR011698. GATase_3. IPR010075. PRibForGlyAmidine_synth_I. [Graphical view]
KO	K01952.
Pfam	PF07685. GATase_3. 1 hit. [Graphical view]
PIRSF	PIRSF001586. FGAM_synth_I. 1 hit.
TIGRFAMs	TIGR01737. FGAM_synth_I. 1 hit.
PROSITE	PS51273. GATASE_TYPE_1. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PURQ_PYRHO

Accession

Primary (citable) accession number: O59619

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 11, 2001
Last sequence update:	July 11, 2001
Last modified:	January 25, 2012
This is version 91 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program