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Protein

Tryptophan--tRNA ligase

Gene

trpS

Organism
Pyrococcus horikoshii (strain ATCC 700860 / DSM 12428 / JCM 9974 / NBRC 100139 / OT-3)
Status
Reviewed-Annotation score: Annotation score: 2 out of 5-Experimental evidence at protein leveli

Functioni

Catalytic activityi

ATP + L-tryptophan + tRNA(Trp) = AMP + diphosphate + L-tryptophyl-tRNA(Trp).UniRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Aminoacyl-tRNA synthetase, Ligase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BRENDAi6.1.1.2. 5244.

Names & Taxonomyi

Protein namesi
Recommended name:
Tryptophan--tRNA ligaseUniRule annotation (EC:6.1.1.2UniRule annotation)
Alternative name(s):
Tryptophanyl-tRNA synthetaseUniRule annotation
Short name:
TrpRSUniRule annotation
Gene namesi
Name:trpSUniRule annotation
Ordered Locus Names:PH1921
OrganismiPyrococcus horikoshii (strain ATCC 700860 / DSM 12428 / JCM 9974 / NBRC 100139 / OT-3)
Taxonomic identifieri70601 [NCBI]
Taxonomic lineageiArchaeaEuryarchaeotaThermococciThermococcalesThermococcaceaePyrococcus
Proteomesi
  • UP000000752 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001367301 – 386Tryptophan--tRNA ligaseAdd BLAST386

Interactioni

Protein-protein interaction databases

STRINGi70601.PH1921.

Structurei

Secondary structure

1386
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi12 – 14Combined sources3
Helixi18 – 24Combined sources7
Helixi32 – 41Combined sources10
Helixi48 – 51Combined sources4
Beta strandi54 – 60Combined sources7
Helixi61 – 69Combined sources9
Beta strandi75 – 80Combined sources6
Helixi88 – 104Combined sources17
Beta strandi107 – 112Combined sources6
Helixi114 – 120Combined sources7
Helixi126 – 141Combined sources16
Beta strandi149 – 154Combined sources6
Turni155 – 157Combined sources3
Helixi160 – 171Combined sources12
Helixi174 – 181Combined sources8
Helixi189 – 199Combined sources11
Helixi200 – 203Combined sources4
Beta strandi204 – 207Combined sources4
Beta strandi209 – 214Combined sources6
Helixi215 – 217Combined sources3
Helixi218 – 227Combined sources10
Helixi229 – 231Combined sources3
Beta strandi237 – 241Combined sources5
Beta strandi250 – 252Combined sources3
Helixi259 – 261Combined sources3
Helixi269 – 277Combined sources9
Helixi302 – 309Combined sources8
Helixi315 – 326Combined sources12
Helixi332 – 357Combined sources26
Helixi361 – 365Combined sources5
Helixi369 – 375Combined sources7

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3JXEX-ray3.00A/B1-386[»]
ProteinModelPortaliO59584.
SMRiO59584.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiO59584.

Family & Domainsi

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi82 – 90"HIGH" region9
Motifi253 – 257"KMSKS" region5

Sequence similaritiesi

Belongs to the class-I aminoacyl-tRNA synthetase family.UniRule annotation

Phylogenomic databases

eggNOGiarCOG01887. Archaea.
COG0180. LUCA.
HOGENOMiHOG000224742.
KOiK01867.
OMAiFFFSHRD.

Family and domain databases

CDDicd00806. TrpRS_core. 1 hit.
Gene3Di3.40.50.620. 1 hit.
HAMAPiMF_00140_A. Trp_tRNA_synth_A. 1 hit.
InterProiIPR001412. aa-tRNA-synth_I_CS.
IPR002305. aa-tRNA-synth_Ic.
IPR014729. Rossmann-like_a/b/a_fold.
IPR002306. Trp-tRNA-ligase.
IPR020653. Tryptophan-tRNA-ligase_arc.
[Graphical view]
PANTHERiPTHR10055. PTHR10055. 1 hit.
PfamiPF00579. tRNA-synt_1b. 1 hit.
[Graphical view]
PRINTSiPR01039. TRNASYNTHTRP.
TIGRFAMsiTIGR00233. trpS. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

O59584-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MVEEFKVTPW EVEGVVDYDK LIKHFGTSPL TEDLLEKTAE LTKSELPIFF
60 70 80 90 100
RRKFFFSHRD YDLILKDYEE GRGFFLYTGR GPSGPMHIGH IIPFFATKWL
110 120 130 140 150
QEKFGVNLYI QITDDEKFLF KENLTFDDTK RWAYDNILDI IAVGFDPDKT
160 170 180 190 200
FIFQNSEFTK IYEMAIPIAK KINFSMAKAV FGFTEQSKIG MIFFPAIQIA
210 220 230 240 250
PTFFERKRCL IPAAIDQDPY WRLQRDFAES LGYYKTAALH SKFVPSLTSL
260 270 280 290 300
SGKMSASKPE TAIYLTDSPE DVEKKVWKFT LTGGRPTLKE QREKGGEPEK
310 320 330 340 350
CVVFKWLEIF FEEDDKKLKE RYYACKNGEL TCGECKRYLI SKIQEFLKEH
360 370 380
QRRRKKAEKL VEKFKYTGKL AQEMWNEAIP EPLKRS
Length:386
Mass (Da):45,305
Last modified:April 16, 2002 - v2
Checksum:i9E3C392F4028B2DD
GO

Sequence cautioni

The sequence BAA31046 differs from that shown. Reason: Erroneous initiation.Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BA000001 Genomic DNA. Translation: BAA31046.1. Different initiation.
PIRiG71206.
RefSeqiWP_010885986.1. NC_000961.1.

Genome annotation databases

EnsemblBacteriaiBAA31046; BAA31046; BAA31046.
GeneIDi1442768.
KEGGipho:PH1921.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BA000001 Genomic DNA. Translation: BAA31046.1. Different initiation.
PIRiG71206.
RefSeqiWP_010885986.1. NC_000961.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3JXEX-ray3.00A/B1-386[»]
ProteinModelPortaliO59584.
SMRiO59584.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi70601.PH1921.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiBAA31046; BAA31046; BAA31046.
GeneIDi1442768.
KEGGipho:PH1921.

Phylogenomic databases

eggNOGiarCOG01887. Archaea.
COG0180. LUCA.
HOGENOMiHOG000224742.
KOiK01867.
OMAiFFFSHRD.

Enzyme and pathway databases

BRENDAi6.1.1.2. 5244.

Miscellaneous databases

EvolutionaryTraceiO59584.

Family and domain databases

CDDicd00806. TrpRS_core. 1 hit.
Gene3Di3.40.50.620. 1 hit.
HAMAPiMF_00140_A. Trp_tRNA_synth_A. 1 hit.
InterProiIPR001412. aa-tRNA-synth_I_CS.
IPR002305. aa-tRNA-synth_Ic.
IPR014729. Rossmann-like_a/b/a_fold.
IPR002306. Trp-tRNA-ligase.
IPR020653. Tryptophan-tRNA-ligase_arc.
[Graphical view]
PANTHERiPTHR10055. PTHR10055. 1 hit.
PfamiPF00579. tRNA-synt_1b. 1 hit.
[Graphical view]
PRINTSiPR01039. TRNASYNTHTRP.
TIGRFAMsiTIGR00233. trpS. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiSYW_PYRHO
AccessioniPrimary (citable) accession number: O59584
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 16, 2002
Last sequence update: April 16, 2002
Last modified: November 30, 2016
This is version 92 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.