Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Non-canonical purine NTP pyrophosphatase

Gene

PH1917

Organism
Pyrococcus horikoshii (strain ATCC 700860 / DSM 12428 / JCM 9974 / NBRC 100139 / OT-3)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Pyrophosphatase that hydrolyzes non-canonical purine nucleotides such as XTP and ITP/dITP to their respective monophosphate derivatives. Might exclude non-canonical purines from DNA precursor pool, thus preventing their incorporation into DNA and avoiding chromosomal lesions (Probable).1 Publication

Catalytic activityi

A nucleoside triphosphate + H2O = a nucleotide + diphosphate.

Cofactori

Mg2+1 Publication, Mn2+1 PublicationNote: Binds 1 divalent metal cation per subunit; can use either Mg2+ or Mn2+.1 Publication

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi36Magnesium or manganese1
Metal bindingi65Magnesium or manganese1
Binding sitei143Substrate1
Binding sitei163Substrate1
Binding sitei169Substrate1

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Keywords - Biological processi

Nucleotide metabolism

Keywords - Ligandi

Magnesium, Manganese, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciPHOR70601:GJWR-1920-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Non-canonical purine NTP pyrophosphatase (EC:3.6.1.9UniRule annotation)
Alternative name(s):
Non-standard purine NTP pyrophosphatase
Nucleoside-triphosphate diphosphatase
Nucleoside-triphosphate pyrophosphatase
Short name:
NTPase
Nucleotide diphosphataseUniRule annotation
Gene namesi
Ordered Locus Names:PH1917
OrganismiPyrococcus horikoshii (strain ATCC 700860 / DSM 12428 / JCM 9974 / NBRC 100139 / OT-3)
Taxonomic identifieri70601 [NCBI]
Taxonomic lineageiArchaeaEuryarchaeotaThermococciThermococcalesThermococcaceaePyrococcus
Proteomesi
  • UP000000752 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00004104281 – 186Non-canonical purine NTP pyrophosphataseAdd BLAST186

Interactioni

Subunit structurei

Homodimer.2 Publications

Protein-protein interaction databases

STRINGi70601.PH1917.

Structurei

Secondary structure

1186
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi2 – 6Combined sources5
Helixi10 – 21Combined sources12
Turni22 – 24Combined sources3
Beta strandi26 – 30Combined sources5
Helixi42 – 53Combined sources12
Turni54 – 56Combined sources3
Beta strandi59 – 70Combined sources12
Helixi71 – 73Combined sources3
Turni74 – 76Combined sources3
Helixi78 – 80Combined sources3
Helixi81 – 87Combined sources7
Helixi89 – 96Combined sources8
Turni97 – 99Combined sources3
Beta strandi104 – 115Combined sources12
Beta strandi118 – 130Combined sources13
Beta strandi137 – 139Combined sources3
Helixi143 – 145Combined sources3
Beta strandi146 – 148Combined sources3
Helixi155 – 157Combined sources3
Helixi160 – 164Combined sources5
Helixi168 – 184Combined sources17

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1V7RX-ray1.40A1-186[»]
2DVNX-ray1.60A/B1-186[»]
2DVOX-ray2.21A1-186[»]
2DVPX-ray1.90A1-186[»]
2E5XX-ray2.00A1-186[»]
2ZTIX-ray2.60A1-186[»]
ProteinModelPortaliO59580.
SMRiO59580.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiO59580.

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni7 – 12Substrate binding6
Regioni65 – 66Substrate binding2

Sequence similaritiesi

Belongs to the HAM1 NTPase family.Curated

Phylogenomic databases

eggNOGiarCOG04184. Archaea.
COG0127. LUCA.
HOGENOMiHOG000293320.
KOiK02428.
OMAiEGFPGPY.

Family and domain databases

CDDicd00515. HAM1. 1 hit.
Gene3Di3.90.950.10. 1 hit.
HAMAPiMF_01405. Non_canon_purine_NTPase. 1 hit.
InterProiIPR002637. Ham1p-like.
IPR029001. ITPase-like_fam.
IPR020922. NTPase.
[Graphical view]
PANTHERiPTHR11067. PTHR11067. 1 hit.
PfamiPF01725. Ham1p_like. 1 hit.
[Graphical view]
SUPFAMiSSF52972. SSF52972. 1 hit.
TIGRFAMsiTIGR00042. TIGR00042. 1 hit.

Sequencei

Sequence statusi: Complete.

O59580-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKIFFITSNP GKVREVANFL GTFGIEIVQL KHEYPEIQAE KLEDVVDFGI
60 70 80 90 100
SWLKGKVPEP FMIEDSGLFI ESLKGFPGVY SSYVYRTIGL EGILKLMEGA
110 120 130 140 150
EDRRAYFKSV IGFYIDGKAY KFSGVTWGRI SNEKRGTHGF GYDPIFIPEG
160 170 180
SEKTFAEMTI EEKNALSHRG KALKAFFEWL KVNLKY
Length:186
Mass (Da):21,205
Last modified:August 1, 1998 - v1
Checksum:i64FD0922B5C74450
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BA000001 Genomic DNA. Translation: BAA31042.1.
PIRiC71206.
RefSeqiWP_010885982.1. NC_000961.1.

Genome annotation databases

EnsemblBacteriaiBAA31042; BAA31042; BAA31042.
GeneIDi1442764.
KEGGipho:PH1917.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BA000001 Genomic DNA. Translation: BAA31042.1.
PIRiC71206.
RefSeqiWP_010885982.1. NC_000961.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1V7RX-ray1.40A1-186[»]
2DVNX-ray1.60A/B1-186[»]
2DVOX-ray2.21A1-186[»]
2DVPX-ray1.90A1-186[»]
2E5XX-ray2.00A1-186[»]
2ZTIX-ray2.60A1-186[»]
ProteinModelPortaliO59580.
SMRiO59580.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi70601.PH1917.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiBAA31042; BAA31042; BAA31042.
GeneIDi1442764.
KEGGipho:PH1917.

Phylogenomic databases

eggNOGiarCOG04184. Archaea.
COG0127. LUCA.
HOGENOMiHOG000293320.
KOiK02428.
OMAiEGFPGPY.

Enzyme and pathway databases

BioCyciPHOR70601:GJWR-1920-MONOMER.

Miscellaneous databases

EvolutionaryTraceiO59580.

Family and domain databases

CDDicd00515. HAM1. 1 hit.
Gene3Di3.90.950.10. 1 hit.
HAMAPiMF_01405. Non_canon_purine_NTPase. 1 hit.
InterProiIPR002637. Ham1p-like.
IPR029001. ITPase-like_fam.
IPR020922. NTPase.
[Graphical view]
PANTHERiPTHR11067. PTHR11067. 1 hit.
PfamiPF01725. Ham1p_like. 1 hit.
[Graphical view]
SUPFAMiSSF52972. SSF52972. 1 hit.
TIGRFAMsiTIGR00042. TIGR00042. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiNTPA_PYRHO
AccessioniPrimary (citable) accession number: O59580
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 28, 2011
Last sequence update: August 1, 1998
Last modified: November 30, 2016
This is version 102 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.