Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Non-canonical purine NTP pyrophosphatase

Gene

PH1917

Organism
Pyrococcus horikoshii (strain ATCC 700860 / DSM 12428 / JCM 9974 / NBRC 100139 / OT-3)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Pyrophosphatase that hydrolyzes non-canonical purine nucleotides such as XTP and ITP/dITP to their respective monophosphate derivatives. Might exclude non-canonical purines from DNA precursor pool, thus preventing their incorporation into DNA and avoiding chromosomal lesions (Probable).1 Publication

Catalytic activityi

A nucleoside triphosphate + H2O = a nucleotide + diphosphate.

Cofactori

Mg2+1 Publication, Mn2+1 PublicationNote: Binds 1 divalent metal cation per subunit; can use either Mg2+ or Mn2+.1 Publication

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi36 – 361Magnesium or manganese
Metal bindingi65 – 651Magnesium or manganese
Binding sitei143 – 1431Substrate
Binding sitei163 – 1631Substrate
Binding sitei169 – 1691Substrate

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Keywords - Biological processi

Nucleotide metabolism

Keywords - Ligandi

Magnesium, Manganese, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciPHOR70601:GJWR-1921-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Non-canonical purine NTP pyrophosphatase (EC:3.6.1.19)
Alternative name(s):
Non-standard purine NTP pyrophosphatase
Nucleoside-triphosphate diphosphatase
Nucleoside-triphosphate pyrophosphatase
Short name:
NTPase
Gene namesi
Ordered Locus Names:PH1917
OrganismiPyrococcus horikoshii (strain ATCC 700860 / DSM 12428 / JCM 9974 / NBRC 100139 / OT-3)
Taxonomic identifieri70601 [NCBI]
Taxonomic lineageiArchaeaEuryarchaeotaThermococciThermococcalesThermococcaceaePyrococcus
Proteomesi
  • UP000000752 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 186186Non-canonical purine NTP pyrophosphatasePRO_0000410428Add
BLAST

Interactioni

Subunit structurei

Homodimer.2 Publications

Protein-protein interaction databases

STRINGi70601.PH1917.

Structurei

Secondary structure

1
186
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi2 – 65Combined sources
Helixi10 – 2112Combined sources
Turni22 – 243Combined sources
Beta strandi26 – 305Combined sources
Helixi42 – 5312Combined sources
Turni54 – 563Combined sources
Beta strandi59 – 7012Combined sources
Helixi71 – 733Combined sources
Turni74 – 763Combined sources
Helixi78 – 803Combined sources
Helixi81 – 877Combined sources
Helixi89 – 968Combined sources
Turni97 – 993Combined sources
Beta strandi104 – 11512Combined sources
Beta strandi118 – 13013Combined sources
Beta strandi137 – 1393Combined sources
Helixi143 – 1453Combined sources
Beta strandi146 – 1483Combined sources
Helixi155 – 1573Combined sources
Helixi160 – 1645Combined sources
Helixi168 – 18417Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1V7RX-ray1.40A1-186[»]
2DVNX-ray1.60A/B1-186[»]
2DVOX-ray2.21A1-186[»]
2DVPX-ray1.90A1-186[»]
2E5XX-ray2.00A1-186[»]
2ZTIX-ray2.60A1-186[»]
ProteinModelPortaliO59580.
SMRiO59580. Positions 1-186.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiO59580.

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni7 – 126Substrate binding
Regioni65 – 662Substrate binding

Sequence similaritiesi

Belongs to the HAM1 NTPase family.Curated

Phylogenomic databases

eggNOGiarCOG04184. Archaea.
COG0127. LUCA.
HOGENOMiHOG000293320.
KOiK02428.
OMAiPYSSYVE.

Family and domain databases

Gene3Di3.90.950.10. 1 hit.
HAMAPiMF_01405. Non_canon_purine_NTPase.
InterProiIPR002637. Ham1p-like.
IPR029001. ITPase-like_fam.
IPR020922. NTPase.
[Graphical view]
PANTHERiPTHR11067. PTHR11067. 1 hit.
PfamiPF01725. Ham1p_like. 1 hit.
[Graphical view]
SUPFAMiSSF52972. SSF52972. 1 hit.
TIGRFAMsiTIGR00042. TIGR00042. 1 hit.

Sequencei

Sequence statusi: Complete.

O59580-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKIFFITSNP GKVREVANFL GTFGIEIVQL KHEYPEIQAE KLEDVVDFGI
60 70 80 90 100
SWLKGKVPEP FMIEDSGLFI ESLKGFPGVY SSYVYRTIGL EGILKLMEGA
110 120 130 140 150
EDRRAYFKSV IGFYIDGKAY KFSGVTWGRI SNEKRGTHGF GYDPIFIPEG
160 170 180
SEKTFAEMTI EEKNALSHRG KALKAFFEWL KVNLKY
Length:186
Mass (Da):21,205
Last modified:August 1, 1998 - v1
Checksum:i64FD0922B5C74450
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BA000001 Genomic DNA. Translation: BAA31042.1.
PIRiC71206.
RefSeqiWP_010885982.1. NC_000961.1.

Genome annotation databases

EnsemblBacteriaiBAA31042; BAA31042; BAA31042.
GeneIDi1442764.
KEGGipho:PH1917.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BA000001 Genomic DNA. Translation: BAA31042.1.
PIRiC71206.
RefSeqiWP_010885982.1. NC_000961.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1V7RX-ray1.40A1-186[»]
2DVNX-ray1.60A/B1-186[»]
2DVOX-ray2.21A1-186[»]
2DVPX-ray1.90A1-186[»]
2E5XX-ray2.00A1-186[»]
2ZTIX-ray2.60A1-186[»]
ProteinModelPortaliO59580.
SMRiO59580. Positions 1-186.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi70601.PH1917.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiBAA31042; BAA31042; BAA31042.
GeneIDi1442764.
KEGGipho:PH1917.

Phylogenomic databases

eggNOGiarCOG04184. Archaea.
COG0127. LUCA.
HOGENOMiHOG000293320.
KOiK02428.
OMAiPYSSYVE.

Enzyme and pathway databases

BioCyciPHOR70601:GJWR-1921-MONOMER.

Miscellaneous databases

EvolutionaryTraceiO59580.

Family and domain databases

Gene3Di3.90.950.10. 1 hit.
HAMAPiMF_01405. Non_canon_purine_NTPase.
InterProiIPR002637. Ham1p-like.
IPR029001. ITPase-like_fam.
IPR020922. NTPase.
[Graphical view]
PANTHERiPTHR11067. PTHR11067. 1 hit.
PfamiPF01725. Ham1p_like. 1 hit.
[Graphical view]
SUPFAMiSSF52972. SSF52972. 1 hit.
TIGRFAMsiTIGR00042. TIGR00042. 1 hit.
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 700860 / DSM 12428 / JCM 9974 / NBRC 100139 / OT-3.
  2. "Structures of dimeric nonstandard nucleotide triphosphate pyrophosphatase from Pyrococcus horikoshii OT3: functional significance of interprotomer conformational changes."
    Lokanath N.K., Pampa K.J., Takio K., Kunishima N.
    J. Mol. Biol. 375:1013-1025(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (1.40 ANGSTROMS) OF APOENZYME AND IN COMPLEXES WITH ITP; IMP AND MN(2+), FUNCTION, COFACTOR, SUBUNIT.
    Strain: ATCC 700860 / DSM 12428 / JCM 9974 / NBRC 100139 / OT-3.
  3. "Structure of nucleotide triphosphate pyrophosphatase from Pyrococcus horikoshii OT3."
    RIKEN structural genomics initiative (RSGI)
    Submitted (FEB-2009) to the PDB data bank
    Cited for: X-RAY CRYSTALLOGRAPHY (2.00 ANGSTROMS) IN COMPLEX WITH ITP.
    Strain: ATCC 700860 / DSM 12428 / JCM 9974 / NBRC 100139 / OT-3.

Entry informationi

Entry nameiNTPA_PYRHO
AccessioniPrimary (citable) accession number: O59580
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 28, 2011
Last sequence update: August 1, 1998
Last modified: December 9, 2015
This is version 98 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.