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Protein

Ribonuclease P protein component 3

Gene

rnp3

Organism
Pyrococcus horikoshii (strain ATCC 700860 / DSM 12428 / JCM 9974 / NBRC 100139 / OT-3)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Part of ribonuclease P, a protein complex that generates mature tRNA molecules by cleaving their 5'-ends. Not absolutely essential for activity in vitro, however it strongly stimulates activity. Binds RNase P RNA.UniRule annotation3 Publications

Catalytic activityi

Endonucleolytic cleavage of RNA, removing 5'-extranucleotides from tRNA precursor.UniRule annotation

Temperature dependencei

Optimum temperature is 70 degrees Celsius.2 Publications

GO - Molecular functioni

  • ribonuclease P activity Source: UniProtKB

GO - Biological processi

  • tRNA 5'-leader removal Source: UniProtKB
Complete GO annotation...

Keywords - Molecular functioni

Endonuclease, Hydrolase, Nuclease

Keywords - Biological processi

tRNA processing

Enzyme and pathway databases

BRENDAi3.1.26.5. 5244.

Names & Taxonomyi

Protein namesi
Recommended name:
Ribonuclease P protein component 3UniRule annotation (EC:3.1.26.5UniRule annotation)
Short name:
RNase P component 3UniRule annotation
Alternative name(s):
Rpp30UniRule annotation
Gene namesi
Name:rnp3UniRule annotation
Ordered Locus Names:PH1877
OrganismiPyrococcus horikoshii (strain ATCC 700860 / DSM 12428 / JCM 9974 / NBRC 100139 / OT-3)
Taxonomic identifieri70601 [NCBI]
Taxonomic lineageiArchaeaEuryarchaeotaThermococciThermococcalesThermococcaceaePyrococcus
Proteomesi
  • UP000000752 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

  • cytoplasm Source: UniProtKB-SubCell
  • ribonuclease P complex Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi42K → A: Fully reconstitutes RNase P activity. 1 Publication1
Mutagenesisi68R → A: 75% reconstituted RNase P activity. 1 Publication1
Mutagenesisi87R → A: Fully reconstitutes RNase P activity. 1 Publication1
Mutagenesisi90R → A: 45% reconstituted RNase P activity. 1 Publication1
Mutagenesisi98D → A: 80% reconstituted RNase P activity. 1 Publication1
Mutagenesisi107R → A: 45% reconstituted RNase P activity. 1 Publication1
Mutagenesisi114H → A: 75% reconstituted RNase P activity. 1 Publication1
Mutagenesisi123K → A: 45% reconstituted RNase P activity. 1 Publication1
Mutagenesisi158K → A: 75% reconstituted RNase P activity. 1 Publication1
Mutagenesisi176R → A: 45% reconstituted RNase P activity. 1 Publication1
Mutagenesisi180D → A: 50% reconstituted RNase P activity. 1 Publication1
Mutagenesisi196K → A: 45% reconstituted RNase P activity. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001400461 – 212Ribonuclease P protein component 3Add BLAST212

Proteomic databases

PRIDEiO59543.

Interactioni

Subunit structurei

Consists of a catalytic RNA component and at least 5 protein subunits. Forms a heterotetrameric subcomplex with Rnp2. Reconstituted enzyme missing individual protein subunits is suboptimally active, showing each subunit contributes to optimization of activity.4 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
rnp2O591502EBI-2603192,EBI-2603177

Protein-protein interaction databases

IntActiO59543. 1 interactor.
STRINGi70601.PH1877.

Structurei

Secondary structure

1212
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi4 – 6Combined sources3
Beta strandi10 – 14Combined sources5
Helixi17 – 26Combined sources10
Beta strandi28 – 38Combined sources11
Helixi42 – 52Combined sources11
Beta strandi55 – 61Combined sources7
Helixi64 – 73Combined sources10
Beta strandi75 – 82Combined sources8
Helixi86 – 94Combined sources9
Beta strandi98 – 101Combined sources4
Turni103 – 106Combined sources4
Beta strandi107 – 109Combined sources3
Helixi114 – 123Combined sources10
Beta strandi126 – 131Combined sources6
Helixi132 – 136Combined sources5
Helixi139 – 159Combined sources21
Beta strandi163 – 166Combined sources4
Helixi172 – 174Combined sources3
Helixi178 – 187Combined sources10
Helixi192 – 197Combined sources6
Turni198 – 200Combined sources3
Helixi201 – 207Combined sources7

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1V77X-ray1.80A1-212[»]
2CZVX-ray2.00A/B1-212[»]
ProteinModelPortaliO59543.
SMRiO59543.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiO59543.

Family & Domainsi

Sequence similaritiesi

Belongs to the eukaryotic/archaeal RNase P protein component 3 family.UniRule annotation

Phylogenomic databases

eggNOGiarCOG00307. Archaea.
COG1603. LUCA.
HOGENOMiHOG000073988.
KOiK03539.
OMAiWFDEVVF.

Family and domain databases

HAMAPiMF_00756. RNase_P_3. 1 hit.
InterProiIPR016195. Pol/histidinol_Pase-like.
IPR023539. RNase_P_comp-3_arc.
IPR002738. RNase_P_p30.
[Graphical view]
PfamiPF01876. RNase_P_p30. 1 hit.
[Graphical view]
SUPFAMiSSF89550. SSF89550. 1 hit.

Sequencei

Sequence statusi: Complete.

O59543-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MVGGGGVKFI EMDIRDKEAY ELAKEWFDEV VVSIKFNEEV DKEKLREARK
60 70 80 90 100
EYGKVAILLS NPKPSLVRDT VQKFKSYLIY VESNDLRVIR YSIEKGVDAI
110 120 130 140 150
ISPWVNRKDP GIDHVLAKLM VKKNVALGFS LRPLLYSNPY ERANLLRFMM
160 170 180 190 200
KAWKLVEKYK VRRFLTSSAQ EKWDVRYPRD LISLGVVIGM EIPQAKASIS
210
MYPEIILKRL KY
Length:212
Mass (Da):24,694
Last modified:August 1, 1998 - v1
Checksum:i321490D4A2859C24
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BA000001 Genomic DNA. Translation: BAA30999.1.
PIRiH71200.
RefSeqiWP_010885939.1. NC_000961.1.

Genome annotation databases

EnsemblBacteriaiBAA30999; BAA30999; BAA30999.
GeneIDi1442721.
KEGGipho:PH1877.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BA000001 Genomic DNA. Translation: BAA30999.1.
PIRiH71200.
RefSeqiWP_010885939.1. NC_000961.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1V77X-ray1.80A1-212[»]
2CZVX-ray2.00A/B1-212[»]
ProteinModelPortaliO59543.
SMRiO59543.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiO59543. 1 interactor.
STRINGi70601.PH1877.

Proteomic databases

PRIDEiO59543.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiBAA30999; BAA30999; BAA30999.
GeneIDi1442721.
KEGGipho:PH1877.

Phylogenomic databases

eggNOGiarCOG00307. Archaea.
COG1603. LUCA.
HOGENOMiHOG000073988.
KOiK03539.
OMAiWFDEVVF.

Enzyme and pathway databases

BRENDAi3.1.26.5. 5244.

Miscellaneous databases

EvolutionaryTraceiO59543.

Family and domain databases

HAMAPiMF_00756. RNase_P_3. 1 hit.
InterProiIPR016195. Pol/histidinol_Pase-like.
IPR023539. RNase_P_comp-3_arc.
IPR002738. RNase_P_p30.
[Graphical view]
PfamiPF01876. RNase_P_p30. 1 hit.
[Graphical view]
SUPFAMiSSF89550. SSF89550. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiRNP3_PYRHO
AccessioniPrimary (citable) accession number: O59543
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 13, 2004
Last sequence update: August 1, 1998
Last modified: November 2, 2016
This is version 100 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.