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Protein

Elongation factor 2

Gene

fusA

Organism
Pyrococcus horikoshii (strain ATCC 700860 / DSM 12428 / JCM 9974 / NBRC 100139 / OT-3)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Catalyzes the GTP-dependent ribosomal translocation step during translation elongation. During this step, the ribosome changes from the pre-translocational (PRE) to the post-translocational (POST) state as the newly formed A-site-bound peptidyl-tRNA and P-site-bound deacylated tRNA move to the P and E sites, respectively. Catalyzes the coordinated movement of the two tRNA molecules, the mRNA and conformational changes in the ribosome (By similarity).By similarity

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi28 – 35GTPBy similarity8
Nucleotide bindingi94 – 98GTPBy similarity5
Nucleotide bindingi148 – 151GTPBy similarity4

GO - Molecular functioni

Keywordsi

Molecular functionElongation factor
Biological processProtein biosynthesis
LigandGTP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciPHOR70601:G1G39-1838-MONOMER

Names & Taxonomyi

Protein namesi
Recommended name:
Elongation factor 2
Short name:
EF-2
Gene namesi
Name:fusA
Synonyms:fus
Ordered Locus Names:PH1899
ORF Names:PHBB002
OrganismiPyrococcus horikoshii (strain ATCC 700860 / DSM 12428 / JCM 9974 / NBRC 100139 / OT-3)
Taxonomic identifieri70601 [NCBI]
Taxonomic lineageiArchaeaEuryarchaeotaThermococciThermococcalesThermococcaceaePyrococcus
Proteomesi
  • UP000000752 Componenti: Chromosome

Subcellular locationi

GO - Cellular componenti

Keywords - Cellular componenti

Cytoplasm

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi597H → A: No histidine modification. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000910441 – 732Elongation factor 2Add BLAST732

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei597Diphthamide1 Publication1

Interactioni

Protein-protein interaction databases

STRINGi70601.PH1899

Structurei

Secondary structure

1732
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi4 – 15Combined sources12
Helixi18 – 20Combined sources3
Beta strandi21 – 29Combined sources9
Beta strandi30 – 33Combined sources4
Helixi34 – 44Combined sources11
Turni45 – 48Combined sources4
Turni68 – 73Combined sources6
Beta strandi75 – 83Combined sources9
Beta strandi86 – 93Combined sources8
Helixi103 – 111Combined sources9
Beta strandi113 – 120Combined sources8
Turni121 – 123Combined sources3
Helixi127 – 138Combined sources12
Beta strandi142 – 148Combined sources7
Helixi150 – 155Combined sources6
Helixi161 – 182Combined sources22
Helixi185 – 187Combined sources3
Turni188 – 191Combined sources4
Turni195 – 198Combined sources4
Beta strandi199 – 204Combined sources6
Turni205 – 208Combined sources4
Beta strandi209 – 211Combined sources3
Helixi213 – 219Combined sources7
Helixi223 – 232Combined sources10
Helixi235 – 241Combined sources7
Helixi244 – 255Combined sources12
Helixi259 – 270Combined sources12
Beta strandi275 – 277Combined sources3
Helixi278 – 284Combined sources7
Beta strandi288 – 290Combined sources3
Beta strandi293 – 302Combined sources10
Turni303 – 305Combined sources3
Beta strandi306 – 319Combined sources14
Beta strandi323 – 326Combined sources4
Turni327 – 330Combined sources4
Beta strandi331 – 334Combined sources4
Beta strandi338 – 342Combined sources5
Beta strandi345 – 348Combined sources4
Beta strandi350 – 353Combined sources4
Beta strandi357 – 362Combined sources6
Beta strandi371 – 375Combined sources5
Beta strandi391 – 399Combined sources9
Helixi400 – 402Combined sources3
Helixi403 – 405Combined sources3
Helixi406 – 416Combined sources11
Turni426 – 428Combined sources3
Beta strandi432 – 437Combined sources6
Helixi438 – 449Combined sources12
Beta strandi456 – 458Combined sources3
Beta strandi466 – 471Combined sources6
Beta strandi476 – 479Combined sources4
Beta strandi486 – 493Combined sources8
Helixi496 – 503Combined sources8
Beta strandi505 – 507Combined sources3
Beta strandi509 – 511Combined sources3
Helixi515 – 524Combined sources10
Helixi529 – 532Combined sources4
Beta strandi535 – 539Combined sources5
Beta strandi542 – 546Combined sources5
Helixi554 – 570Combined sources17
Turni573 – 575Combined sources3
Beta strandi581 – 590Combined sources10
Helixi595 – 597Combined sources3
Helixi600 – 616Combined sources17
Beta strandi620 – 634Combined sources15
Helixi635 – 637Combined sources3
Helixi638 – 647Combined sources10
Beta strandi651 – 658Combined sources8
Beta strandi661 – 669Combined sources9
Helixi670 – 672Combined sources3
Helixi676 – 683Combined sources8
Turni684 – 686Combined sources3
Beta strandi689 – 699Combined sources11
Helixi702 – 715Combined sources14
Helixi725 – 728Combined sources4

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
5H7JX-ray2.30A/B1-732[»]
5H7KX-ray1.60A1-386[»]
5H7LX-ray3.10A/B1-732[»]
ProteinModelPortaliO59521
SMRiO59521
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini19 – 260tr-type GAdd BLAST242

Sequence similaritiesi

Phylogenomic databases

eggNOGiarCOG01559 Archaea
COG0480 LUCA
HOGENOMiHOG000231589
KOiK03234
OMAiEHLISGM
OrthoDBiPOG093Z00HF

Family and domain databases

Gene3Di3.30.230.10, 2 hits
HAMAPiMF_00054_A EF_G_EF_2_A, 1 hit
InterProiView protein in InterPro
IPR035647 EFG_III/V
IPR000640 EFG_V-like
IPR004161 EFTu-like_2
IPR031157 G_TR_CS
IPR027417 P-loop_NTPase
IPR020568 Ribosomal_S5_D2-typ_fold
IPR014721 Ribosomal_S5_D2-typ_fold_subgr
IPR005225 Small_GTP-bd_dom
IPR000795 TF_GTP-bd_dom
IPR009000 Transl_B-barrel_sf
IPR004543 Transl_elong_EFG/EF2_arc
IPR005517 Transl_elong_EFG/EF2_IV
PfamiView protein in Pfam
PF00679 EFG_C, 1 hit
PF03764 EFG_IV, 1 hit
PF00009 GTP_EFTU, 1 hit
PF03144 GTP_EFTU_D2, 1 hit
PRINTSiPR00315 ELONGATNFCT
SMARTiView protein in SMART
SM00838 EFG_C, 1 hit
SM00889 EFG_IV, 1 hit
SUPFAMiSSF50447 SSF50447, 1 hit
SSF52540 SSF52540, 1 hit
SSF54211 SSF54211, 1 hit
SSF54980 SSF54980, 2 hits
TIGRFAMsiTIGR00490 aEF-2, 1 hit
TIGR00231 small_GTP, 1 hit
PROSITEiView protein in PROSITE
PS00301 G_TR_1, 1 hit
PS51722 G_TR_2, 1 hit

Sequencei

Sequence statusi: Complete.

O59521-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MGRREEMIAK IKELMLQPER IRNIGIAAHI DHGKTTLSDN LLAGAGMISE
60 70 80 90 100
ELAGKQLVLD FDEQEQARGI TINAANVSMV HNYEGKDYLI NLIDTPGHVD
110 120 130 140 150
FGGDVTRAMR AIDGVIIVVD AVEGVMPQTE TVVRQALREY VKPVLFINKV
160 170 180 190 200
DRLIRELKLT PQQMMERFSK IIMDVNRLIQ RYAPEEYKKK WMVKVEDGSV
210 220 230 240 250
AFGSAYYNWA LSVPFMKRTG VKFNEIIDLT LKGDNRTLRQ KAPLHVVVLD
260 270 280 290 300
MVVRHLPSPI EAQKYRIPHL WEGDISSDIG QAMLNCDPKG KMVMVVTKII
310 320 330 340 350
IDKHAGEVAT GRVWSGTVKS GQEVYLINTK RKARIQQVGI YMGPERINME
360 370 380 390 400
AVPAGNIVAV TGLRDAMAGE TVAEEQIEPF EALHYVSEPV VTVAIEAKNV
410 420 430 440 450
KDLPRLIEAL RQLAKEDPTL HVKIDEETGQ HLLSGMGELH LEVKLYKLKK
460 470 480 490 500
DWGIDIEVSE PIVVYRESIT KSSPMVEGKS PNRHNRFYIV VEPMPDEIYN
510 520 530 540 550
AIKEGIIPEG RVKNPKEVAK KLAELGMDYE IARGIVDIYN GNMFIDNTKG
560 570 580 590 600
VQYLNEVMDL LIDGFHQAMD EGPLAREPVM KVIVRLLDAQ VHEDNVHRGP
610 620 630 640 650
AQIYPAIRTA IHCAMMKSNP VLYEPYQKVI INIPYEYMGA VSREITQRRG
660 670 680 690 700
QLVDMKQEGE VMTIIAEAPV AEMFGFAGSI RSATSGRALW STEHAGFKRV
710 720 730
PNELAQQIIR QIRQRKGLDP NPPTEKDVCP LF
Length:732
Mass (Da):82,399
Last modified:May 30, 2000 - v2
Checksum:i6914AFBAA6DEA09D
GO

Sequence cautioni

The sequence BAA31022 differs from that shown. Reason: Erroneous initiation.Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BA000001 Genomic DNA Translation: BAA31022.1 Different initiation.
PIRiG71203
RefSeqiWP_048053510.1, NC_000961.1

Genome annotation databases

EnsemblBacteriaiBAA31022; BAA31022; BAA31022
GeneIDi1442743
KEGGipho:PH1899

Similar proteinsi

Entry informationi

Entry nameiEF2_PYRHO
AccessioniPrimary (citable) accession number: O59521
Entry historyiIntegrated into UniProtKB/Swiss-Prot: December 15, 1998
Last sequence update: May 30, 2000
Last modified: May 23, 2018
This is version 119 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

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