Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Elongation factor 2

Gene

fusA

Organism
Pyrococcus horikoshii (strain ATCC 700860 / DSM 12428 / JCM 9974 / NBRC 100139 / OT-3)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Catalyzes the GTP-dependent ribosomal translocation step during translation elongation. During this step, the ribosome changes from the pre-translocational (PRE) to the post-translocational (POST) state as the newly formed A-site-bound peptidyl-tRNA and P-site-bound deacylated tRNA move to the P and E sites, respectively. Catalyzes the coordinated movement of the two tRNA molecules, the mRNA and conformational changes in the ribosome (By similarity).By similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi28 – 358GTPBy similarity
Nucleotide bindingi94 – 985GTPBy similarity
Nucleotide bindingi148 – 1514GTPBy similarity

GO - Molecular functioni

Complete GO annotation...

Keywords - Molecular functioni

Elongation factor

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

GTP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciPHOR70601:GJWR-1900-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Elongation factor 2
Short name:
EF-2
Gene namesi
Name:fusA
Synonyms:fus
Ordered Locus Names:PH1899
ORF Names:PHBB002
OrganismiPyrococcus horikoshii (strain ATCC 700860 / DSM 12428 / JCM 9974 / NBRC 100139 / OT-3)
Taxonomic identifieri70601 [NCBI]
Taxonomic lineageiArchaeaEuryarchaeotaThermococciThermococcalesThermococcaceaePyrococcus
Proteomesi
  • UP000000752 Componenti: Chromosome

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi597 – 5971H → A: No histidine modification. 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 732732Elongation factor 2PRO_0000091044Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei597 – 5971Diphthamide1 Publication

Interactioni

Protein-protein interaction databases

STRINGi70601.PH1899.

Structurei

3D structure databases

ProteinModelPortaliO59521.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini19 – 260242tr-type GAdd
BLAST

Sequence similaritiesi

Phylogenomic databases

eggNOGiarCOG01559. Archaea.
COG0480. LUCA.
HOGENOMiHOG000231589.
KOiK03234.
OMAiVMIIISE.

Family and domain databases

Gene3Di3.30.230.10. 1 hit.
3.30.70.240. 1 hit.
3.40.50.300. 2 hits.
HAMAPiMF_00054_A. EF_G_EF_2_A. 1 hit.
InterProiIPR009022. EFG_III-V.
IPR000640. EFG_V.
IPR004161. EFTu-like_2.
IPR031157. G_TR_CS.
IPR027417. P-loop_NTPase.
IPR020568. Ribosomal_S5_D2-typ_fold.
IPR014721. Ribosomal_S5_D2-typ_fold_subgr.
IPR005225. Small_GTP-bd_dom.
IPR000795. TF_GTP-bd_dom.
IPR009000. Transl_B-barrel.
IPR004543. Transl_elong_EFG/EF2_arc.
IPR005517. Transl_elong_EFG/EF2_IV.
[Graphical view]
PfamiPF00679. EFG_C. 1 hit.
PF14492. EFG_II. 1 hit.
PF03764. EFG_IV. 1 hit.
PF03144. GTP_EFTU_D2. 1 hit.
[Graphical view]
PRINTSiPR00315. ELONGATNFCT.
SMARTiSM00838. EFG_C. 1 hit.
SM00889. EFG_IV. 1 hit.
[Graphical view]
SUPFAMiSSF50447. SSF50447. 1 hit.
SSF52540. SSF52540. 1 hit.
SSF54211. SSF54211. 1 hit.
SSF54980. SSF54980. 2 hits.
TIGRFAMsiTIGR00490. aEF-2. 1 hit.
TIGR00231. small_GTP. 1 hit.
PROSITEiPS00301. G_TR_1. 1 hit.
PS51722. G_TR_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

O59521-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MGRREEMIAK IKELMLQPER IRNIGIAAHI DHGKTTLSDN LLAGAGMISE
60 70 80 90 100
ELAGKQLVLD FDEQEQARGI TINAANVSMV HNYEGKDYLI NLIDTPGHVD
110 120 130 140 150
FGGDVTRAMR AIDGVIIVVD AVEGVMPQTE TVVRQALREY VKPVLFINKV
160 170 180 190 200
DRLIRELKLT PQQMMERFSK IIMDVNRLIQ RYAPEEYKKK WMVKVEDGSV
210 220 230 240 250
AFGSAYYNWA LSVPFMKRTG VKFNEIIDLT LKGDNRTLRQ KAPLHVVVLD
260 270 280 290 300
MVVRHLPSPI EAQKYRIPHL WEGDISSDIG QAMLNCDPKG KMVMVVTKII
310 320 330 340 350
IDKHAGEVAT GRVWSGTVKS GQEVYLINTK RKARIQQVGI YMGPERINME
360 370 380 390 400
AVPAGNIVAV TGLRDAMAGE TVAEEQIEPF EALHYVSEPV VTVAIEAKNV
410 420 430 440 450
KDLPRLIEAL RQLAKEDPTL HVKIDEETGQ HLLSGMGELH LEVKLYKLKK
460 470 480 490 500
DWGIDIEVSE PIVVYRESIT KSSPMVEGKS PNRHNRFYIV VEPMPDEIYN
510 520 530 540 550
AIKEGIIPEG RVKNPKEVAK KLAELGMDYE IARGIVDIYN GNMFIDNTKG
560 570 580 590 600
VQYLNEVMDL LIDGFHQAMD EGPLAREPVM KVIVRLLDAQ VHEDNVHRGP
610 620 630 640 650
AQIYPAIRTA IHCAMMKSNP VLYEPYQKVI INIPYEYMGA VSREITQRRG
660 670 680 690 700
QLVDMKQEGE VMTIIAEAPV AEMFGFAGSI RSATSGRALW STEHAGFKRV
710 720 730
PNELAQQIIR QIRQRKGLDP NPPTEKDVCP LF
Length:732
Mass (Da):82,399
Last modified:May 30, 2000 - v2
Checksum:i6914AFBAA6DEA09D
GO

Sequence cautioni

The sequence BAA31022 differs from that shown. Reason: Erroneous initiation. Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BA000001 Genomic DNA. Translation: BAA31022.1. Different initiation.
PIRiG71203.
RefSeqiWP_048053510.1. NC_000961.1.

Genome annotation databases

EnsemblBacteriaiBAA31022; BAA31022; BAA31022.
GeneIDi1442743.
KEGGipho:PH1899.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BA000001 Genomic DNA. Translation: BAA31022.1. Different initiation.
PIRiG71203.
RefSeqiWP_048053510.1. NC_000961.1.

3D structure databases

ProteinModelPortaliO59521.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi70601.PH1899.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiBAA31022; BAA31022; BAA31022.
GeneIDi1442743.
KEGGipho:PH1899.

Phylogenomic databases

eggNOGiarCOG01559. Archaea.
COG0480. LUCA.
HOGENOMiHOG000231589.
KOiK03234.
OMAiVMIIISE.

Enzyme and pathway databases

BioCyciPHOR70601:GJWR-1900-MONOMER.

Family and domain databases

Gene3Di3.30.230.10. 1 hit.
3.30.70.240. 1 hit.
3.40.50.300. 2 hits.
HAMAPiMF_00054_A. EF_G_EF_2_A. 1 hit.
InterProiIPR009022. EFG_III-V.
IPR000640. EFG_V.
IPR004161. EFTu-like_2.
IPR031157. G_TR_CS.
IPR027417. P-loop_NTPase.
IPR020568. Ribosomal_S5_D2-typ_fold.
IPR014721. Ribosomal_S5_D2-typ_fold_subgr.
IPR005225. Small_GTP-bd_dom.
IPR000795. TF_GTP-bd_dom.
IPR009000. Transl_B-barrel.
IPR004543. Transl_elong_EFG/EF2_arc.
IPR005517. Transl_elong_EFG/EF2_IV.
[Graphical view]
PfamiPF00679. EFG_C. 1 hit.
PF14492. EFG_II. 1 hit.
PF03764. EFG_IV. 1 hit.
PF03144. GTP_EFTU_D2. 1 hit.
[Graphical view]
PRINTSiPR00315. ELONGATNFCT.
SMARTiSM00838. EFG_C. 1 hit.
SM00889. EFG_IV. 1 hit.
[Graphical view]
SUPFAMiSSF50447. SSF50447. 1 hit.
SSF52540. SSF52540. 1 hit.
SSF54211. SSF54211. 1 hit.
SSF54980. SSF54980. 2 hits.
TIGRFAMsiTIGR00490. aEF-2. 1 hit.
TIGR00231. small_GTP. 1 hit.
PROSITEiPS00301. G_TR_1. 1 hit.
PS51722. G_TR_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiEF2_PYRHO
AccessioniPrimary (citable) accession number: O59521
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 15, 1998
Last sequence update: May 30, 2000
Last modified: July 6, 2016
This is version 106 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.