Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Glyceraldehyde-3-phosphate dehydrogenase

Gene

gap

Organism
Pyrococcus horikoshii (strain ATCC 700860 / DSM 12428 / JCM 9974 / NBRC 100139 / OT-3)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Catalytic activityi

D-glyceraldehyde 3-phosphate + phosphate + NAD(P)+ = 3-phospho-D-glyceroyl phosphate + NAD(P)H.

Pathwayi: glycolysis

This protein is involved in step 1 of the subpathway that synthesizes pyruvate from D-glyceraldehyde 3-phosphate.
Proteins known to be involved in the 5 steps of the subpathway in this organism are:
  1. Glyceraldehyde-3-phosphate dehydrogenase (gap)
  2. Phosphoglycerate kinase (pgk)
  3. 2,3-bisphosphoglycerate-independent phosphoglycerate mutase (apgM)
  4. Enolase (eno)
  5. Pyruvate kinase (PH0570)
This subpathway is part of the pathway glycolysis, which is itself part of Carbohydrate degradation.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes pyruvate from D-glyceraldehyde 3-phosphate, the pathway glycolysis and in Carbohydrate degradation.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei111 – 1111NAD; via amide nitrogen1 Publication
Active sitei141 – 1411NucleophileBy similarity
Binding sitei167 – 1671NAD1 Publication
Binding sitei298 – 2981NAD; via carbonyl oxygen1 Publication

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi12 – 132NAD1 Publication

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

Glycolysis

Keywords - Ligandi

NAD, NADP

Enzyme and pathway databases

BioCyciPHOR70601:GJWR-1827-MONOMER.
UniPathwayiUPA00109; UER00184.

Names & Taxonomyi

Protein namesi
Recommended name:
Glyceraldehyde-3-phosphate dehydrogenase (EC:1.2.1.59)
Short name:
GAPDH
Alternative name(s):
NAD(P)-dependent glyceraldehyde-3-phosphate dehydrogenase
Gene namesi
Name:gap
Ordered Locus Names:PH1830
OrganismiPyrococcus horikoshii (strain ATCC 700860 / DSM 12428 / JCM 9974 / NBRC 100139 / OT-3)
Taxonomic identifieri70601 [NCBI]
Taxonomic lineageiArchaeaEuryarchaeotaThermococciThermococcalesThermococcaceaePyrococcus
Proteomesi
  • UP000000752 Componenti: Chromosome

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 334334Glyceraldehyde-3-phosphate dehydrogenasePRO_0000145731Add
BLAST

Interactioni

Subunit structurei

Homotetramer.1 Publication

Protein-protein interaction databases

STRINGi70601.PH1830.

Structurei

Secondary structure

1
334
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi3 – 86Combined sources
Helixi12 – 2211Combined sources
Beta strandi27 – 3610Combined sources
Helixi39 – 468Combined sources
Beta strandi51 – 555Combined sources
Helixi56 – 583Combined sources
Helixi59 – 657Combined sources
Helixi73 – 775Combined sources
Beta strandi81 – 855Combined sources
Helixi91 – 10212Combined sources
Beta strandi105 – 1084Combined sources
Helixi114 – 1163Combined sources
Beta strandi117 – 1215Combined sources
Helixi123 – 1264Combined sources
Helixi127 – 1304Combined sources
Beta strandi134 – 1385Combined sources
Helixi141 – 15313Combined sources
Helixi154 – 1563Combined sources
Beta strandi157 – 16913Combined sources
Beta strandi187 – 1893Combined sources
Helixi192 – 1965Combined sources
Turni197 – 1993Combined sources
Beta strandi204 – 2129Combined sources
Beta strandi217 – 22711Combined sources
Helixi231 – 2399Combined sources
Beta strandi244 – 2474Combined sources
Helixi249 – 2513Combined sources
Helixi256 – 26510Combined sources
Helixi269 – 2713Combined sources
Beta strandi275 – 2795Combined sources
Helixi280 – 2823Combined sources
Beta strandi284 – 2863Combined sources
Beta strandi289 – 2968Combined sources
Turni298 – 3025Combined sources
Helixi303 – 31311Combined sources
Helixi319 – 33012Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2CZCX-ray2.00A/B/C/D1-334[»]
ProteinModelPortaliO59494.
SMRiO59494. Positions 1-334.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiO59494.

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni140 – 1423Glyceraldehyde 3-phosphate bindingBy similarity
Regioni192 – 1932Glyceraldehyde 3-phosphate bindingBy similarity

Sequence similaritiesi

Phylogenomic databases

eggNOGiarCOG00493. Archaea.
COG0057. LUCA.
HOGENOMiHOG000223361.
KOiK00150.
OMAiVTVPSHH.

Family and domain databases

Gene3Di3.40.50.720. 1 hit.
HAMAPiMF_00559. G3P_dehdrog_arch. 1 hit.
InterProiIPR000846. DapB_N.
IPR020831. GlycerAld/Erythrose_P_DH.
IPR020830. GlycerAld_3-P_DH_AS.
IPR020829. GlycerAld_3-P_DH_cat.
IPR020828. GlycerAld_3-P_DH_NAD(P)-bd.
IPR006436. Glyceraldehyde-3-P_DH_2_arc.
IPR016040. NAD(P)-bd_dom.
[Graphical view]
PfamiPF01113. DapB_N. 1 hit.
PF02800. Gp_dh_C. 1 hit.
[Graphical view]
PIRSFiPIRSF000149. GAP_DH. 1 hit.
SMARTiSM00846. Gp_dh_N. 1 hit.
[Graphical view]
SUPFAMiSSF51735. SSF51735. 1 hit.
TIGRFAMsiTIGR01546. GAPDH-II_archae. 1 hit.
PROSITEiPS00071. GAPDH. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

O59494-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKVKVGVNGY GTIGKRVAYA VTKQDDMELI GITKTKPDFE AYRAKELGIP
60 70 80 90 100
VYAASEEFIP RFEKEGFEVA GTLNDLLEKV DIIVDATPGG IGAKNKPLYE
110 120 130 140 150
KAGVKAIFQG GEKADVAEVS FVAQANYEAA LGKNYVRVVS CNTTGLVRTL
160 170 180 190 200
SAIREYADYV YAVMIRRAAD PNDTKRGPIN AIKPTVEVPS HHGPDVQTVI
210 220 230 240 250
PINIETMAFV VPTTLMHVHS VMVELKKPLT KDDVIDIFEN TTRVLLFEKE
260 270 280 290 300
KGFDSTAQII EFARDLHREW NNLYEIAVWK ESINIKGNRL FYIQAVHQES
310 320 330
DVIPENIDAI RAMFELADKW DSIKKTNKSL GILK
Length:334
Mass (Da):37,264
Last modified:August 1, 1998 - v1
Checksum:iAEA615734718E39B
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BA000001 Genomic DNA. Translation: BAA30950.1.
PIRiG71194.
RefSeqiWP_010885890.1. NC_000961.1.

Genome annotation databases

EnsemblBacteriaiBAA30950; BAA30950; BAA30950.
GeneIDi1442672.
KEGGipho:PH1830.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BA000001 Genomic DNA. Translation: BAA30950.1.
PIRiG71194.
RefSeqiWP_010885890.1. NC_000961.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2CZCX-ray2.00A/B/C/D1-334[»]
ProteinModelPortaliO59494.
SMRiO59494. Positions 1-334.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi70601.PH1830.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiBAA30950; BAA30950; BAA30950.
GeneIDi1442672.
KEGGipho:PH1830.

Phylogenomic databases

eggNOGiarCOG00493. Archaea.
COG0057. LUCA.
HOGENOMiHOG000223361.
KOiK00150.
OMAiVTVPSHH.

Enzyme and pathway databases

UniPathwayiUPA00109; UER00184.
BioCyciPHOR70601:GJWR-1827-MONOMER.

Miscellaneous databases

EvolutionaryTraceiO59494.

Family and domain databases

Gene3Di3.40.50.720. 1 hit.
HAMAPiMF_00559. G3P_dehdrog_arch. 1 hit.
InterProiIPR000846. DapB_N.
IPR020831. GlycerAld/Erythrose_P_DH.
IPR020830. GlycerAld_3-P_DH_AS.
IPR020829. GlycerAld_3-P_DH_cat.
IPR020828. GlycerAld_3-P_DH_NAD(P)-bd.
IPR006436. Glyceraldehyde-3-P_DH_2_arc.
IPR016040. NAD(P)-bd_dom.
[Graphical view]
PfamiPF01113. DapB_N. 1 hit.
PF02800. Gp_dh_C. 1 hit.
[Graphical view]
PIRSFiPIRSF000149. GAP_DH. 1 hit.
SMARTiSM00846. Gp_dh_N. 1 hit.
[Graphical view]
SUPFAMiSSF51735. SSF51735. 1 hit.
TIGRFAMsiTIGR01546. GAPDH-II_archae. 1 hit.
PROSITEiPS00071. GAPDH. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiG3P_PYRHO
AccessioniPrimary (citable) accession number: O59494
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 30, 2000
Last sequence update: August 1, 1998
Last modified: December 9, 2015
This is version 112 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.