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Protein

tRNA (guanine(26)-N(2))-dimethyltransferase

Gene

trm1

Organism
Pyrococcus horikoshii (strain ATCC 700860 / DSM 12428 / JCM 9974 / NBRC 100139 / OT-3)
Status
Reviewed-Annotation score: Annotation score: 2 out of 5-Experimental evidence at protein leveli

Functioni

Dimethylates a single guanine residue at position 26 of a number of tRNAs using S-adenosyl-L-methionine as donor of the methyl groups.UniRule annotation

Catalytic activityi

2 S-adenosyl-L-methionine + guanine(26) in tRNA = 2 S-adenosyl-L-homocysteine + N(2)-dimethylguanine(26) in tRNA.UniRule annotation

GO - Molecular functioni

Complete GO annotation...

Keywords - Molecular functioni

Methyltransferase, Transferase

Keywords - Biological processi

tRNA processing

Keywords - Ligandi

RNA-binding, S-adenosyl-L-methionine, tRNA-binding

Enzyme and pathway databases

BRENDAi2.1.1.216. 5244.

Names & Taxonomyi

Protein namesi
Recommended name:
tRNA (guanine(26)-N(2))-dimethyltransferaseUniRule annotation (EC:2.1.1.216UniRule annotation)
Alternative name(s):
tRNA 2,2-dimethylguanosine-26 methyltransferaseUniRule annotation
tRNA(guanine-26,N(2)-N(2)) methyltransferaseUniRule annotation
tRNA(m(2,2)G26)dimethyltransferaseUniRule annotation
Gene namesi
Name:trm1UniRule annotation
Ordered Locus Names:PH1829
OrganismiPyrococcus horikoshii (strain ATCC 700860 / DSM 12428 / JCM 9974 / NBRC 100139 / OT-3)
Taxonomic identifieri70601 [NCBI]
Taxonomic lineageiArchaeaEuryarchaeotaThermococciThermococcalesThermococcaceaePyrococcus
Proteomesi
  • UP000000752 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001476901 – 381tRNA (guanine(26)-N(2))-dimethyltransferaseAdd BLAST381

Interactioni

Protein-protein interaction databases

STRINGi70601.PH1829.

Structurei

Secondary structure

1381
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi7 – 11Combined sources5
Beta strandi14 – 18Combined sources5
Turni24 – 26Combined sources3
Beta strandi27 – 29Combined sources3
Helixi33 – 35Combined sources3
Helixi36 – 49Combined sources14
Beta strandi52 – 58Combined sources7
Helixi63 – 71Combined sources9
Beta strandi75 – 82Combined sources8
Helixi84 – 97Combined sources14
Beta strandi103 – 105Combined sources3
Beta strandi106 – 122Combined sources17
Helixi124 – 130Combined sources7
Beta strandi135 – 140Combined sources6
Helixi147 – 156Combined sources10
Beta strandi157 – 167Combined sources11
Helixi170 – 173Combined sources4
Helixi178 – 185Combined sources8
Beta strandi186 – 188Combined sources3
Helixi195 – 212Combined sources18
Turni213 – 215Combined sources3
Beta strandi216 – 226Combined sources11
Beta strandi229 – 239Combined sources11
Helixi240 – 247Combined sources8
Beta strandi250 – 255Combined sources6
Turni257 – 259Combined sources3
Beta strandi262 – 269Combined sources8
Beta strandi272 – 274Combined sources3
Beta strandi276 – 280Combined sources5
Helixi287 – 298Combined sources12
Helixi305 – 317Combined sources13
Helixi327 – 334Combined sources8
Helixi341 – 350Combined sources10
Beta strandi355 – 358Combined sources4
Beta strandi361 – 369Combined sources9
Helixi371 – 378Combined sources8

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2DULX-ray1.90A4-381[»]
2EJTX-ray2.20A4-381[»]
2EJUX-ray1.95A4-381[»]
2YTZX-ray2.65A/B4-381[»]
ProteinModelPortaliO59493.
SMRiO59493.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiO59493.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini7 – 378Trm1 methyltransferaseUniRule annotationAdd BLAST372

Sequence similaritiesi

Belongs to the class I-like SAM-binding methyltransferase superfamily. Trm1 family.UniRule annotation
Contains 1 Trm1 methyltransferase domain.UniRule annotation

Phylogenomic databases

eggNOGiarCOG01219. Archaea.
COG1867. LUCA.
HOGENOMiHOG000229931.
KOiK00555.
OMAiFYNPRMA.

Family and domain databases

Gene3Di3.40.50.150. 1 hit.
HAMAPiMF_00290. tRNA_dimethyltr_TRM1. 1 hit.
InterProiIPR029063. SAM-dependent_MTases.
IPR002905. Trm1.
IPR022923. TRM1_arc_bac.
[Graphical view]
PANTHERiPTHR10631. PTHR10631. 1 hit.
PfamiPF02005. TRM. 1 hit.
[Graphical view]
SUPFAMiSSF53335. SSF53335. 1 hit.
TIGRFAMsiTIGR00308. TRM1. 1 hit.
PROSITEiPS51626. SAM_MT_TRM1. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

O59493-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MVNLELIEVQ EGKAKILIPK AESIYDSPVF YNPRMALNRD IVVVLLNILN
60 70 80 90 100
PKIVLDALSA TGIRGIRFAL ETPAEEVWLN DISEDAYELM KRNVMLNFDG
110 120 130 140 150
ELRESKGRAI LKGEKTIVIN HDDANRLMAE RHRYFHFIDL DPFGSPMEFL
160 170 180 190 200
DTALRSAKRR GILGVTATDG APLCGAHPRA CLRKYLAVPL RGELCHEVGT
210 220 230 240 250
RILVGVIARY AAKYDLGIDV ILAYYKDHYF RAFVKLKDGA RKGDETLEKL
260 270 280 290 300
GYIYFDDKTG KFELEQGFLP TRPNAYGPVW LGPLKDEKIV SKMVKEAESL
310 320 330 340 350
SLARKKQALK LLKMIDQELD IPLFYDTHAI GRRLKIETKK VEEIISALRE
360 370 380
QGYEATRTHF SPTGIKTSAP YEVFIETIKR I
Length:381
Mass (Da):43,271
Last modified:August 1, 1998 - v1
Checksum:i7D28AB17F805450E
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BA000001 Genomic DNA. Translation: BAA30948.1.
PIRiE71194.

Genome annotation databases

EnsemblBacteriaiBAA30948; BAA30948; BAA30948.
KEGGipho:PH1829.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BA000001 Genomic DNA. Translation: BAA30948.1.
PIRiE71194.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2DULX-ray1.90A4-381[»]
2EJTX-ray2.20A4-381[»]
2EJUX-ray1.95A4-381[»]
2YTZX-ray2.65A/B4-381[»]
ProteinModelPortaliO59493.
SMRiO59493.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi70601.PH1829.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiBAA30948; BAA30948; BAA30948.
KEGGipho:PH1829.

Phylogenomic databases

eggNOGiarCOG01219. Archaea.
COG1867. LUCA.
HOGENOMiHOG000229931.
KOiK00555.
OMAiFYNPRMA.

Enzyme and pathway databases

BRENDAi2.1.1.216. 5244.

Miscellaneous databases

EvolutionaryTraceiO59493.

Family and domain databases

Gene3Di3.40.50.150. 1 hit.
HAMAPiMF_00290. tRNA_dimethyltr_TRM1. 1 hit.
InterProiIPR029063. SAM-dependent_MTases.
IPR002905. Trm1.
IPR022923. TRM1_arc_bac.
[Graphical view]
PANTHERiPTHR10631. PTHR10631. 1 hit.
PfamiPF02005. TRM. 1 hit.
[Graphical view]
SUPFAMiSSF53335. SSF53335. 1 hit.
TIGRFAMsiTIGR00308. TRM1. 1 hit.
PROSITEiPS51626. SAM_MT_TRM1. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiTRM1_PYRHO
AccessioniPrimary (citable) accession number: O59493
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 11, 2001
Last sequence update: August 1, 1998
Last modified: November 2, 2016
This is version 99 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.