Skip Header

Contribute Send feedback
Read comments (?) or add your own

O59493 (TRM1_PYRHO) Reviewed, UniProtKB/Swiss-Prot

Last modified January 25, 2012. Version 70. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
tRNA (guanine(26)-N(2))-dimethyltransferase
EC=2.1.1.216
Alternative name(s):
tRNA 2,2-dimethylguanosine-26 methyltransferase
tRNA(guanine-26,N(2)-N(2)) methyltransferase
tRNA(m(2,2)G26)dimethyltransferase
Gene names
Name:trm1
Ordered Locus Names:PH1829
OrganismPyrococcus horikoshii (strain ATCC 700860 / DSM 12428 / JCM 9974 / NBRC 100139 / OT-3) [Complete proteome] [HAMAP]
Taxonomic identifier70601 [NCBI]
Taxonomic lineageArchaea › Euryarchaeota › Thermococci › Thermococcales › Thermococcaceae › Pyrococcus

Protein attributes

Sequence length381 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Dimethylates a single guanine residue at position 26 of a number of tRNAs using S-adenosyl-L-methionine as donor of the methyl groups By similarity. HAMAP MF_00290

Catalytic activity

2 S-adenosyl-L-methionine + guanine(26) in tRNA = 2 S-adenosyl-L-homocysteine + N(2)-dimethylguanine(26) in tRNA. HAMAP MF_00290

Sequence similarities

Belongs to the TRM1 family.

Ontologies

Keywords
   Biological processtRNA processing
   LigandS-adenosyl-L-methionine
   Molecular functionMethyltransferase
Transferase
   Technical term3D-structure
Complete proteome
Gene Ontology (GO)
   Molecular functionRNA binding

Inferred from electronic annotation. Source: InterPro

tRNA (guanine-N2-)-methyltransferase activity

Inferred from electronic annotation. Source: InterPro

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 381381tRNA (guanine(26)-N(2))-dimethyltransferase HAMAP MF_00290
PRO_0000147690

Secondary structure

1 ............................................................. 381
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
O59493 [UniParc].

Last modified August 1, 1998. Version 1.
Checksum: 7D28AB17F805450E

FASTA38143,271
        10         20         30         40         50         60 
MVNLELIEVQ EGKAKILIPK AESIYDSPVF YNPRMALNRD IVVVLLNILN PKIVLDALSA 

        70         80         90        100        110        120 
TGIRGIRFAL ETPAEEVWLN DISEDAYELM KRNVMLNFDG ELRESKGRAI LKGEKTIVIN 

       130        140        150        160        170        180 
HDDANRLMAE RHRYFHFIDL DPFGSPMEFL DTALRSAKRR GILGVTATDG APLCGAHPRA 

       190        200        210        220        230        240 
CLRKYLAVPL RGELCHEVGT RILVGVIARY AAKYDLGIDV ILAYYKDHYF RAFVKLKDGA 

       250        260        270        280        290        300 
RKGDETLEKL GYIYFDDKTG KFELEQGFLP TRPNAYGPVW LGPLKDEKIV SKMVKEAESL 

       310        320        330        340        350        360 
SLARKKQALK LLKMIDQELD IPLFYDTHAI GRRLKIETKK VEEIISALRE QGYEATRTHF 

       370        380 
SPTGIKTSAP YEVFIETIKR I

« Hide

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		BA000001 Genomic DNA. Translation: BAA30948.1.
PIRE71194.
RefSeqNP_143660.1. NC_000961.1.

3D structure databases

PDBe
RCSB PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
2DULX-ray1.90A5-381[»]
2EJTX-ray2.20A5-381[»]
2EJUX-ray1.95A5-381[»]
2YTZX-ray2.65A/B5-381[»]
ProteinModelPortalO59493.
SMRO59493. Positions 6-380.
ModBaseSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaEBPYRT00000000284; EBPYRP00000000284; EBPYRG00000000284.
GeneID1442670.
GenomeReviewsGene locus PH1829 in contig BA000001_GR.
KEGGpho:PH1829.
NMPDRfig|70601.1.peg.1784.

Organism-specific databases

CMRSearch...

Phylogenomic databases

GeneTreeEBGT00050000022754.
HOGENOMHBG577872.
OMACEPLSGT.
PhylomeDBO59493.
ProtClustDBPRK04338.

Enzyme and pathway databases

BioCycPHOR70601:PH1829-MONOMER.

Family and domain databases

HAMAPMF_00290. tRNA_dimethyltr_TRM1.
[Tree]
InterProIPR022923. DimeGuo_tRNA_meTrfase_arc.
IPR002905. TRM_MeTrfase.
[Graphical view]
KOK00555.
PANTHERPTHR10631. TRM_mtfrase. 1 hit.
PfamPF02005. TRM. 1 hit.
[Graphical view]
TIGRFAMsTIGR00308. TRM1. 1 hit.
ProtoNetSearch...

Entry information

Entry nameTRM1_PYRHO
AccessionPrimary (citable) accession number: O59493
Entry history
Integrated into UniProtKB/Swiss-Prot: January 11, 2001
Last sequence update: August 1, 1998
Last modified: January 25, 2012
This is version 70 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

SIMILARITY comments

Index of protein domains and families

to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents