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Protein

tRNA (guanine(26)-N(2))-dimethyltransferase

Gene

trm1

Organism
Pyrococcus horikoshii (strain ATCC 700860 / DSM 12428 / JCM 9974 / NBRC 100139 / OT-3)
Status
Reviewed-Annotation score: Annotation score: 2 out of 5-Experimental evidence at protein leveli

Functioni

Dimethylates a single guanine residue at position 26 of a number of tRNAs using S-adenosyl-L-methionine as donor of the methyl groups.UniRule annotation

Catalytic activityi

2 S-adenosyl-L-methionine + guanine(26) in tRNA = 2 S-adenosyl-L-homocysteine + N(2)-dimethylguanine(26) in tRNA.UniRule annotation

GO - Molecular functioni

Complete GO annotation...

Keywords - Molecular functioni

Methyltransferase, Transferase

Keywords - Biological processi

tRNA processing

Keywords - Ligandi

RNA-binding, S-adenosyl-L-methionine, tRNA-binding

Enzyme and pathway databases

BioCyciPHOR70601:GJWR-1825-MONOMER.
BRENDAi2.1.1.216. 5244.

Names & Taxonomyi

Protein namesi
Recommended name:
tRNA (guanine(26)-N(2))-dimethyltransferaseUniRule annotation (EC:2.1.1.216UniRule annotation)
Alternative name(s):
tRNA 2,2-dimethylguanosine-26 methyltransferaseUniRule annotation
tRNA(guanine-26,N(2)-N(2)) methyltransferaseUniRule annotation
tRNA(m(2,2)G26)dimethyltransferaseUniRule annotation
Gene namesi
Name:trm1UniRule annotation
Ordered Locus Names:PH1829
OrganismiPyrococcus horikoshii (strain ATCC 700860 / DSM 12428 / JCM 9974 / NBRC 100139 / OT-3)
Taxonomic identifieri70601 [NCBI]
Taxonomic lineageiArchaeaEuryarchaeotaThermococciThermococcalesThermococcaceaePyrococcus
Proteomesi
  • UP000000752 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 381381tRNA (guanine(26)-N(2))-dimethyltransferasePRO_0000147690Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi70601.PH1829.

Structurei

Secondary structure

1
381
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi7 – 115Combined sources
Beta strandi14 – 185Combined sources
Turni24 – 263Combined sources
Beta strandi27 – 293Combined sources
Helixi33 – 353Combined sources
Helixi36 – 4914Combined sources
Beta strandi52 – 587Combined sources
Helixi63 – 719Combined sources
Beta strandi75 – 828Combined sources
Helixi84 – 9714Combined sources
Beta strandi103 – 1053Combined sources
Beta strandi106 – 12217Combined sources
Helixi124 – 1307Combined sources
Beta strandi135 – 1406Combined sources
Helixi147 – 15610Combined sources
Beta strandi157 – 16711Combined sources
Helixi170 – 1734Combined sources
Helixi178 – 1858Combined sources
Beta strandi186 – 1883Combined sources
Helixi195 – 21218Combined sources
Turni213 – 2153Combined sources
Beta strandi216 – 22611Combined sources
Beta strandi229 – 23911Combined sources
Helixi240 – 2478Combined sources
Beta strandi250 – 2556Combined sources
Turni257 – 2593Combined sources
Beta strandi262 – 2698Combined sources
Beta strandi272 – 2743Combined sources
Beta strandi276 – 2805Combined sources
Helixi287 – 29812Combined sources
Helixi305 – 31713Combined sources
Helixi327 – 3348Combined sources
Helixi341 – 35010Combined sources
Beta strandi355 – 3584Combined sources
Beta strandi361 – 3699Combined sources
Helixi371 – 3788Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2DULX-ray1.90A4-381[»]
2EJTX-ray2.20A4-381[»]
2EJUX-ray1.95A4-381[»]
2YTZX-ray2.65A/B4-381[»]
ProteinModelPortaliO59493.
SMRiO59493. Positions 6-380.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiO59493.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini7 – 378372Trm1 methyltransferaseUniRule annotationAdd
BLAST

Sequence similaritiesi

Belongs to the class I-like SAM-binding methyltransferase superfamily. Trm1 family.UniRule annotation
Contains 1 Trm1 methyltransferase domain.UniRule annotation

Phylogenomic databases

eggNOGiarCOG01219. Archaea.
COG1867. LUCA.
HOGENOMiHOG000229931.
KOiK00555.
OMAiFYNPRMA.

Family and domain databases

Gene3Di3.40.50.150. 1 hit.
HAMAPiMF_00290. tRNA_dimethyltr_TRM1. 1 hit.
InterProiIPR029063. SAM-dependent_MTases.
IPR002905. Trm1.
IPR022923. TRM1_arc_bac.
[Graphical view]
PANTHERiPTHR10631. PTHR10631. 1 hit.
PfamiPF02005. TRM. 1 hit.
[Graphical view]
SUPFAMiSSF53335. SSF53335. 1 hit.
TIGRFAMsiTIGR00308. TRM1. 1 hit.
PROSITEiPS51626. SAM_MT_TRM1. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

O59493-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MVNLELIEVQ EGKAKILIPK AESIYDSPVF YNPRMALNRD IVVVLLNILN
60 70 80 90 100
PKIVLDALSA TGIRGIRFAL ETPAEEVWLN DISEDAYELM KRNVMLNFDG
110 120 130 140 150
ELRESKGRAI LKGEKTIVIN HDDANRLMAE RHRYFHFIDL DPFGSPMEFL
160 170 180 190 200
DTALRSAKRR GILGVTATDG APLCGAHPRA CLRKYLAVPL RGELCHEVGT
210 220 230 240 250
RILVGVIARY AAKYDLGIDV ILAYYKDHYF RAFVKLKDGA RKGDETLEKL
260 270 280 290 300
GYIYFDDKTG KFELEQGFLP TRPNAYGPVW LGPLKDEKIV SKMVKEAESL
310 320 330 340 350
SLARKKQALK LLKMIDQELD IPLFYDTHAI GRRLKIETKK VEEIISALRE
360 370 380
QGYEATRTHF SPTGIKTSAP YEVFIETIKR I
Length:381
Mass (Da):43,271
Last modified:August 1, 1998 - v1
Checksum:i7D28AB17F805450E
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BA000001 Genomic DNA. Translation: BAA30948.1.
PIRiE71194.

Genome annotation databases

EnsemblBacteriaiBAA30948; BAA30948; BAA30948.
KEGGipho:PH1829.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BA000001 Genomic DNA. Translation: BAA30948.1.
PIRiE71194.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2DULX-ray1.90A4-381[»]
2EJTX-ray2.20A4-381[»]
2EJUX-ray1.95A4-381[»]
2YTZX-ray2.65A/B4-381[»]
ProteinModelPortaliO59493.
SMRiO59493. Positions 6-380.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi70601.PH1829.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiBAA30948; BAA30948; BAA30948.
KEGGipho:PH1829.

Phylogenomic databases

eggNOGiarCOG01219. Archaea.
COG1867. LUCA.
HOGENOMiHOG000229931.
KOiK00555.
OMAiFYNPRMA.

Enzyme and pathway databases

BioCyciPHOR70601:GJWR-1825-MONOMER.
BRENDAi2.1.1.216. 5244.

Miscellaneous databases

EvolutionaryTraceiO59493.

Family and domain databases

Gene3Di3.40.50.150. 1 hit.
HAMAPiMF_00290. tRNA_dimethyltr_TRM1. 1 hit.
InterProiIPR029063. SAM-dependent_MTases.
IPR002905. Trm1.
IPR022923. TRM1_arc_bac.
[Graphical view]
PANTHERiPTHR10631. PTHR10631. 1 hit.
PfamiPF02005. TRM. 1 hit.
[Graphical view]
SUPFAMiSSF53335. SSF53335. 1 hit.
TIGRFAMsiTIGR00308. TRM1. 1 hit.
PROSITEiPS51626. SAM_MT_TRM1. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiTRM1_PYRHO
AccessioniPrimary (citable) accession number: O59493
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 11, 2001
Last sequence update: August 1, 1998
Last modified: September 7, 2016
This is version 97 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.