ID   HMDH_PYRHO              Reviewed;         413 AA.
AC   O59469;
DT   01-DEC-2000, integrated into UniProtKB/Swiss-Prot.
DT   01-DEC-2000, sequence version 2.
DT   16-JUN-2009, entry version 60.
DE   RecName: Full=3-hydroxy-3-methylglutaryl-coenzyme A reductase;
DE            Short=HMG-CoA reductase;
DE            EC=1.1.1.34;
GN   Name=hmgA; OrderedLocusNames=PH1805;
OS   Pyrococcus horikoshii.
OC   Archaea; Euryarchaeota; Thermococci; Thermococcales; Thermococcaceae;
OC   Pyrococcus.
OX   NCBI_TaxID=53953;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=OT3;
RX   MEDLINE=98344137; PubMed=9679194; DOI=10.1093/dnares/5.2.55;
RA   Kawarabayasi Y., Sawada M., Horikawa H., Haikawa Y., Hino Y.,
RA   Yamamoto S., Sekine M., Baba S., Kosugi H., Hosoyama A., Nagai Y.,
RA   Sakai M., Ogura K., Otsuka R., Nakazawa H., Takamiya M., Ohfuku Y.,
RA   Funahashi T., Tanaka T., Kudoh Y., Yamazaki J., Kushida N., Oguchi A.,
RA   Aoki K., Yoshizawa T., Nakamura Y., Robb F.T., Horikoshi K.,
RA   Masuchi Y., Shizuya H., Kikuchi H.;
RT   "Complete sequence and gene organization of the genome of a hyper-
RT   thermophilic archaebacterium, Pyrococcus horikoshii OT3.";
RL   DNA Res. 5:55-76(1998).
CC   -!- FUNCTION: Converts HMG-CoA to mevalonate (By similarity).
CC   -!- CATALYTIC ACTIVITY: (R)-mevalonate + CoA + 2 NADP(+) = (S)-3-
CC       hydroxy-3-methylglutaryl-CoA + 2 NADPH.
CC   -!- PATHWAY: Metabolic intermediate biosynthesis; mevalonic acid
CC       biosynthesis; (R)-mevalonic acid from acetyl-CoA: step 3/3.
CC   -!- SIMILARITY: Belongs to the HMG-CoA reductase family.
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DR   EMBL; BA000001; BAA30924.1; ALT_INIT; Genomic_DNA.
DR   PIR; E71191; E71191.
DR   RefSeq; NP_143640.1; -.
DR   HSSP; P04035; 1HWI.
DR   GeneID; 1442647; -.
DR   GenomeReviews; BA000001_GR; PH1805.
DR   KEGG; pho:PH1805; -.
DR   NMPDR; fig|70601.1.peg.1761; -.
DR   HOGENOM; O59469; -.
DR   OMA; O59469; AHFANII.
DR   BRENDA; 1.1.1.34; 74679.
DR   GO; GO:0016021; C:integral to membrane; IEA:InterPro.
DR   GO; GO:0004420; F:hydroxymethylglutaryl-CoA reductase (NADPH)...; IEA:EC.
DR   GO; GO:0050661; F:NADP or NADPH binding; IEA:InterPro.
DR   GO; GO:0015936; P:coenzyme A metabolic process; IEA:InterPro.
DR   GO; GO:0008299; P:isoprenoid biosynthetic process; IEA:UniProtKB-KW.
DR   GO; GO:0055114; P:oxidation reduction; IEA:UniProtKB-KW.
DR   InterPro; IPR002202; HMG_CoA_Rdtase_cat.
DR   InterPro; IPR004554; HMG_CoA_Rdtase_I_cat.
DR   Gene3D; G3DSA:3.90.770.10; HMG-CoA_red; 1.
DR   PANTHER; PTHR10572; HMG-CoA_red; 1.
DR   Pfam; PF00368; HMG-CoA_red; 1.
DR   PRINTS; PR00071; HMGCOARDTASE.
DR   TIGRFAMs; TIGR00533; HMG_CoA_R_NADP; 1.
DR   PROSITE; PS00066; HMG_COA_REDUCTASE_1; 1.
DR   PROSITE; PS00318; HMG_COA_REDUCTASE_2; 1.
DR   PROSITE; PS01192; HMG_COA_REDUCTASE_3; FALSE_NEG.
DR   PROSITE; PS50065; HMG_COA_REDUCTASE_4; 1.
PE   3: Inferred from homology;
KW   Complete proteome; Isoprene biosynthesis; NADP; Oxidoreductase.
FT   CHAIN         1    413       3-hydroxy-3-methylglutaryl-coenzyme A
FT                                reductase.
FT                                /FTId=PRO_0000114464.
FT   ACT_SITE    106    106       Charge relay system (By similarity).
FT   ACT_SITE    312    312       Charge relay system (By similarity).
FT   ACT_SITE    408    408       Proton donor (By similarity).
SQ   SEQUENCE   413 AA;  44554 MW;  3FF84B70DA984B84 CRC64;
     MGGGNLNIEE IIEKVANGEI KFYQVEKYVN GDKRLATEIR RKALEKRLGI KLHHIGYYSI
     DPNELIGRNI ENMIGVVQIP MGVAGPLKIN GEYAKGEFYI PLATTEGALV ASVNRGCSAL
     TEAGGVVTTL IDDKMTRAPL IRCPNARRAR EVAKWVEENL DYLQEKAVSK VTRHGKLRGV
     KPFIVGNNLY LRFEFETGDA MGMNMVTIAS EEIMKVIEEE FPDVRYLALS GNLCVDKKPN
     AVNFILGRGK TVIAEAVVPR KIVEKKLKTT PELIAEVNYF KNLVGSAQAG SYGFNAHFAN
     IVGAIFLATG QDEAQITEGA HGITIAEVTP DGDLYISITM PSLEIGTVGG GTRVPSQREA
     LEIMGVAGGG DPPGINAKKF AEIVAGAVLA GELSLLAAIA AKHLARAHKM LGR
//