Reviewed, UniProtKB/Swiss-Prot O59469 (HMDH_PYRHO)

Names and origin

Protein names

Recommended name:
    3-hydroxy-3-methylglutaryl-coenzyme A reductase
      Short name=HMG-CoA reductase
    EC=1.1.1.34

Gene names

Name:	hmgA
Ordered Locus Names:	PH1805

Organism

Pyrococcus horikoshii [Complete proteome] [HAMAP]

Taxonomic identifier

53953 [NCBI]

Taxonomic lineage

Archaea › Euryarchaeota › Thermococci › Thermococcales › Thermococcaceae › Pyrococcus

Protein attributes

Sequence length	413 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Converts HMG-CoA to mevalonate By similarity.
Catalytic activity	(R)-mevalonate + CoA + 2 NADP(+) = (S)-3-hydroxy-3-methylglutaryl-CoA + 2 NADPH.
Pathway	Metabolic intermediate biosynthesis; mevalonic acid biosynthesis; (R)-mevalonic acid from acetyl-CoA: step 3/3.
Sequence similarities	Belongs to the HMG-CoA reductase family.

Ontologies

Keywords
Biological process	Isoprene biosynthesis
Ligand	NADP
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	coenzyme A metabolic process Inferred from electronic annotation. Source: InterPro isoprenoid biosynthetic process Inferred from electronic annotation. Source: InterPro oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	integral to membrane Inferred from electronic annotation. Source: InterPro
Molecular function	NADP binding Inferred from electronic annotation. Source: InterPro hydroxymethylglutaryl-CoA reductase (NADPH) activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 413	413	3-hydroxy-3-methylglutaryl-coenzyme A reductase		PRO_0000114464
Sites
	Active site	106	1	Charge relay system By similarity
	Active site	312	1	Charge relay system By similarity
	Active site	408	1	Proton donor By similarity

Sequences

	Sequence		Length	Mass (Da)
	O59469-1 [UniParc]. Last modified December 1, 2000. Version 2. Checksum: 3FF84B70DA984B84	FASTA	413	44,554
	10 20 30 40 50 60 MGGGNLNIEE IIEKVANGEI KFYQVEKYVN GDKRLATEIR RKALEKRLGI KLHHIGYYSI 70 80 90 100 110 120 DPNELIGRNI ENMIGVVQIP MGVAGPLKIN GEYAKGEFYI PLATTEGALV ASVNRGCSAL 130 140 150 160 170 180 TEAGGVVTTL IDDKMTRAPL IRCPNARRAR EVAKWVEENL DYLQEKAVSK VTRHGKLRGV 190 200 210 220 230 240 KPFIVGNNLY LRFEFETGDA MGMNMVTIAS EEIMKVIEEE FPDVRYLALS GNLCVDKKPN 250 260 270 280 290 300 AVNFILGRGK TVIAEAVVPR KIVEKKLKTT PELIAEVNYF KNLVGSAQAG SYGFNAHFAN 310 320 330 340 350 360 IVGAIFLATG QDEAQITEGA HGITIAEVTP DGDLYISITM PSLEIGTVGG GTRVPSQREA 370 380 390 400 410 LEIMGVAGGG DPPGINAKKF AEIVAGAVLA GELSLLAAIA AKHLARAHKM LGR

References

[1]

"Complete sequence and gene organization of the genome of a hyper-thermophilic archaebacterium, Pyrococcus horikoshii OT3." Kawarabayasi Y., Sawada M., Horikawa H., Haikawa Y., Hino Y., Yamamoto S., Sekine M., Baba S., Kosugi H., Hosoyama A., Nagai Y., Sakai M., Ogura K., Otsuka R., Nakazawa H., Takamiya M., Ohfuku Y., Funahashi T. , Tanaka T., Kudoh Y., Yamazaki J., Kushida N., Oguchi A., Aoki K., Yoshizawa T., Nakamura Y., Robb F.T., Horikoshi K., Masuchi Y., Shizuya H., Kikuchi H. DNA Res. 5:55-76(1998) [PubMed: 9679194] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: OT3.

Cross-references

Sequence databases
EMBLGenBankDDBJ	BA000001 Genomic DNA. Translation: BAA30924.1. Different initiation.
PIR	E71191.
RefSeq	NP_143640.1.
3D structure databases
HSSP	HSSP built from PDB template 1HWI based on UniProtKB P04035.
ModBase	Search...
Genome annotation databases
GeneID	1442647.
GenomeReviews	Gene locus PH1805 in contig BA000001_GR.
KEGG	pho:PH1805.
NMPDR	fig|70601.1.peg.1761.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	O59469.
Family and domain databases
InterPro	IPR002202. HMG_CoA_Rdtase_cat. IPR004554. HMG_CoA_Rdtase_I_cat. [Graphical view]
Gene3D	G3DSA:3.90.770.10. HMG-CoA_red. 1 hit.
PANTHER	PTHR10572. HMG-CoA_red. 1 hit.
Pfam	PF00368. HMG-CoA_red. 1 hit. [Graphical view]
PRINTS	PR00071. HMGCOARDTASE.
TIGRFAMs	TIGR00533. HMG_CoA_R_NADP. 1 hit.
PROSITE	PS00066. HMG_COA_REDUCTASE_1. 1 hit. PS00318. HMG_COA_REDUCTASE_2. 1 hit. PS01192. HMG_COA_REDUCTASE_3. False negative. PS50065. HMG_COA_REDUCTASE_4. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

HMDH_PYRHO

Accession

Primary (citable) accession number: O59469

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 1, 2000
Last sequence update:	December 1, 2000
Last modified:	November 25, 2008
This is version 56 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)