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Protein

CTP synthase

Gene

pyrG

Organism
Pyrococcus horikoshii (strain ATCC 700860 / DSM 12428 / JCM 9974 / NBRC 100139 / OT-3)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the ATP-dependent amination of UTP to CTP with either L-glutamine or ammonia as the source of nitrogen.UniRule annotation

Catalytic activityi

ATP + UTP + L-glutamine = ADP + phosphate + CTP + L-glutamate.UniRule annotation

Enzyme regulationi

Allosterically activated by GTP, when glutamine is the substrate. Inhibited by CTP.UniRule annotation

Pathwayi: CTP biosynthesis via de novo pathway

This protein is involved in step 2 of the subpathway that synthesizes CTP from UDP.UniRule annotation
Proteins known to be involved in the 2 steps of the subpathway in this organism are:
  1. no protein annotated in this organism
  2. CTP synthase (pyrG)
This subpathway is part of the pathway CTP biosynthesis via de novo pathway, which is itself part of Pyrimidine metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes CTP from UDP, the pathway CTP biosynthesis via de novo pathway and in Pyrimidine metabolism.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei378 – 3781NucleophileUniRule annotation
Active sitei505 – 5051UniRule annotation
Active sitei507 – 5071UniRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Ligase

Keywords - Biological processi

Pyrimidine biosynthesis

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciPHOR70601:GJWR-1792-MONOMER.
UniPathwayiUPA00159; UER00277.

Protein family/group databases

MEROPSiC26.964.

Names & Taxonomyi

Protein namesi
Recommended name:
CTP synthaseUniRule annotation (EC:6.3.4.2UniRule annotation)
Alternative name(s):
CTP synthetaseUniRule annotation
UTP--ammonia ligaseUniRule annotation
Gene namesi
Name:pyrGUniRule annotation
Ordered Locus Names:PH1792
OrganismiPyrococcus horikoshii (strain ATCC 700860 / DSM 12428 / JCM 9974 / NBRC 100139 / OT-3)
Taxonomic identifieri70601 [NCBI]
Taxonomic lineageiArchaeaEuryarchaeotaThermococciThermococcalesThermococcaceaePyrococcus
Proteomesi
  • UP000000752 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 537537CTP synthasePRO_0000138267Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi70601.PH1792.

Structurei

3D structure databases

ProteinModelPortaliO59456.
SMRiO59456. Positions 2-531.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini290 – 532243Glutamine amidotransferase type-1UniRule annotationAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni1 – 252252Aminator domainAdd
BLAST

Sequence similaritiesi

Belongs to the CTP synthase family.UniRule annotation
Contains 1 glutamine amidotransferase type-1 domain.UniRule annotation

Keywords - Domaini

Glutamine amidotransferase

Phylogenomic databases

eggNOGiarCOG00063. Archaea.
COG0504. LUCA.
HOGENOMiHOG000077514.
KOiK01937.
OMAiFDHNITT.

Family and domain databases

Gene3Di3.40.50.300. 1 hit.
3.40.50.880. 1 hit.
HAMAPiMF_01227. PyrG.
InterProiIPR029062. Class_I_gatase-like.
IPR004468. CTP_synthase.
IPR017456. CTP_synthase_N.
IPR017926. GATASE.
IPR027417. P-loop_NTPase.
[Graphical view]
PANTHERiPTHR11550. PTHR11550. 1 hit.
PfamiPF06418. CTP_synth_N. 1 hit.
PF00117. GATase. 1 hit.
[Graphical view]
SUPFAMiSSF52317. SSF52317. 1 hit.
SSF52540. SSF52540. 1 hit.
TIGRFAMsiTIGR00337. PyrG. 1 hit.
PROSITEiPS51273. GATASE_TYPE_1. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

O59456-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTKFIFVTGG VVSGLGKGIT SASIGLLMKA RGYKTTNIKI DPYINYDAGT
60 70 80 90 100
MNPYQHGEVF VLDDGGEVDL DLGNYERFLD TNLTFEHNIT TGKVYSTVIE
110 120 130 140 150
KERRGEYLGA TVQVIPHITD EIKRRIREIA KDYDIVVVEI GGTVGDIESM
160 170 180 190 200
PFLEAARQMQ LEEGRENVAF VHVTYVPKLK VVGEQKTKPT QHSVKELRSL
210 220 230 240 250
GIQPDAIVAR SEDPLEEEAR KKISLFTNVP REAVVSAYDV EDTYEVPLLL
260 270 280 290 300
EREGLGKYLI KRLKLEDREP DLREWEKMVA KYKALKETVE IAIVGKYVKL
310 320 330 340 350
TDSYLSIKEA LKHASVSNDV KVKIRWIEAE DIEEHGTKLL EGVDGIIVPG
360 370 380 390 400
GFGARGAEGK IMTIKYAREN DIPFLGICFG FQLTVVEFAR NVLGMKGAHS
410 420 430 440 450
TEIDPQTPYP VVDLMPEQRN LEKLGGTMRL GAYPVKIKKG TLAYRLYKKE
460 470 480 490 500
LVYERHRHRW EVNPDYIEAF EKAGLVFSGV AGDDERRMEI LELPDKRYFI
510 520 530
ATQFHPEFKS RPMRPAPVFH GLVRAAKEYK QEKNATN
Length:537
Mass (Da):60,803
Last modified:October 19, 2002 - v2
Checksum:iD15DDB10CFF29004
GO

Sequence cautioni

The sequence BAA30911.1 differs from that shown. Reason: Erroneous initiation. Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BA000001 Genomic DNA. Translation: BAA30911.1. Different initiation.
PIRiH71189.
RefSeqiWP_010885855.1. NC_000961.1.

Genome annotation databases

EnsemblBacteriaiBAA30911; BAA30911; BAA30911.
GeneIDi1442637.
KEGGipho:PH1792.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BA000001 Genomic DNA. Translation: BAA30911.1. Different initiation.
PIRiH71189.
RefSeqiWP_010885855.1. NC_000961.1.

3D structure databases

ProteinModelPortaliO59456.
SMRiO59456. Positions 2-531.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi70601.PH1792.

Protein family/group databases

MEROPSiC26.964.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiBAA30911; BAA30911; BAA30911.
GeneIDi1442637.
KEGGipho:PH1792.

Phylogenomic databases

eggNOGiarCOG00063. Archaea.
COG0504. LUCA.
HOGENOMiHOG000077514.
KOiK01937.
OMAiFDHNITT.

Enzyme and pathway databases

UniPathwayiUPA00159; UER00277.
BioCyciPHOR70601:GJWR-1792-MONOMER.

Family and domain databases

Gene3Di3.40.50.300. 1 hit.
3.40.50.880. 1 hit.
HAMAPiMF_01227. PyrG.
InterProiIPR029062. Class_I_gatase-like.
IPR004468. CTP_synthase.
IPR017456. CTP_synthase_N.
IPR017926. GATASE.
IPR027417. P-loop_NTPase.
[Graphical view]
PANTHERiPTHR11550. PTHR11550. 1 hit.
PfamiPF06418. CTP_synth_N. 1 hit.
PF00117. GATase. 1 hit.
[Graphical view]
SUPFAMiSSF52317. SSF52317. 1 hit.
SSF52540. SSF52540. 1 hit.
TIGRFAMsiTIGR00337. PyrG. 1 hit.
PROSITEiPS51273. GATASE_TYPE_1. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 700860 / DSM 12428 / JCM 9974 / NBRC 100139 / OT-3.

Entry informationi

Entry nameiPYRG_PYRHO
AccessioniPrimary (citable) accession number: O59456
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 19, 2002
Last sequence update: October 19, 2002
Last modified: November 11, 2015
This is version 97 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.