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O59425

- RNP1_PYRHO

UniProt

O59425 - RNP1_PYRHO

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Protein

Ribonuclease P protein component 1

Gene

rnp1

Organism
Pyrococcus horikoshii (strain ATCC 700860 / DSM 12428 / JCM 9974 / NBRC 100139 / OT-3)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

Part of ribonuclease P (RNase P), a protein complex that generates mature tRNA molecules by cleaving their 5'-ends. Binds RNase P RNA.4 Publications

Catalytic activityi

Endonucleolytic cleavage of RNA, removing 5'-extranucleotides from tRNA precursor.UniRule annotation

Temperature dependencei

Optimum temperature is 70 degrees Celsius.2 Publications

GO - Molecular functioni

  1. ribonuclease P activity Source: UniProtKB
  2. RNA binding Source: InterPro

GO - Biological processi

  1. mRNA cleavage Source: InterPro
  2. RNA phosphodiester bond hydrolysis Source: GOC
  3. RNA phosphodiester bond hydrolysis, endonucleolytic Source: GOC
  4. rRNA processing Source: InterPro
  5. tRNA 5'-leader removal Source: UniProtKB
Complete GO annotation...

Keywords - Molecular functioni

Endonuclease, Hydrolase, Nuclease

Keywords - Biological processi

tRNA processing

Enzyme and pathway databases

BioCyciPHOR70601:GJWR-1769-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Ribonuclease P protein component 1UniRule annotation (EC:3.1.26.5UniRule annotation)
Short name:
RNase P component 1UniRule annotation
Alternative name(s):
Rpp29UniRule annotation
Gene namesi
Name:rnp1UniRule annotation
Ordered Locus Names:PH1771
OrganismiPyrococcus horikoshii (strain ATCC 700860 / DSM 12428 / JCM 9974 / NBRC 100139 / OT-3)
Taxonomic identifieri70601 [NCBI]
Taxonomic lineageiArchaeaEuryarchaeotaThermococciThermococcalesThermococcaceaePyrococcus
ProteomesiUP000000752: Chromosome

Subcellular locationi

Cytoplasm UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-HAMAP
  2. ribonuclease MRP complex Source: InterPro
  3. ribonuclease P complex Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi1 – 3131Missing: Does not reconstitute RNase P activity, no longer interacts with Rnp4. 1 PublicationAdd
BLAST
Mutagenesisi47 – 471E → A: Fully reconstitutes RNase P activity. 1 Publication
Mutagenesisi73 – 731E → A: 40% reconstituted RNase P activity. 1 Publication
Mutagenesisi75 – 751R → A: 40% reconstituted RNase P activity. 1 Publication
Mutagenesisi90 – 901K → A: 10% reconstituted RNase P activity. 1 Publication
Mutagenesisi95 – 951F → A: 75% reconstituted RNase P activity. 1 Publication
Mutagenesisi115 – 1151R → A: 75% reconstituted RNase P activity. 1 Publication
Mutagenesisi118 – 12710Missing: Does not reconstitute RNase P activity. 1 Publication
Mutagenesisi121 – 1222KK → AA: 60% reconstituted RNase P activity. 1 Publication
Mutagenesisi121 – 1211K → A: 90% reconstituted RNase P activity. 1 Publication
Mutagenesisi122 – 1221K → A: Fully reconstitutes RNase P activity. 1 Publication
Mutagenesisi124 – 1274Missing: Fully reconstitutes RNase P activity. 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 127127Ribonuclease P protein component 1PRO_0000128437Add
BLAST

Interactioni

Subunit structurei

Consists of a catalytic RNA component and at least 5 protein subunits. Forms a heterodimeric subcomplex with Rnp4. Reconstituted enzyme missing individual protein subunits is suboptimally active, showing each subunit contributes to optimization of activity.4 Publications

Protein-protein interaction databases

IntActiO59425. 1 interaction.
STRINGi70601.PH1771.

Structurei

Secondary structure

1
127
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi21 – 233Combined sources
Helixi27 – 304Combined sources
Turni31 – 333Combined sources
Helixi42 – 443Combined sources
Beta strandi52 – 609Combined sources
Helixi61 – 633Combined sources
Beta strandi67 – 737Combined sources
Beta strandi75 – 9016Combined sources
Beta strandi93 – 986Combined sources
Beta strandi104 – 1085Combined sources
Helixi109 – 1124Combined sources
Helixi116 – 1194Combined sources
Helixi120 – 1234Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1V76X-ray2.00A/B32-127[»]
2ZAEX-ray2.21A/C1-127[»]
ProteinModelPortaliO59425.
SMRiO59425. Positions 36-127.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiO59425.

Family & Domainsi

Sequence similaritiesi

Belongs to the eukaryotic/archaeal RNase P protein component 1 family.UniRule annotation

Phylogenomic databases

eggNOGiCOG1588.
HOGENOMiHOG000231353.
KOiK03538.
OMAiISTKGRV.

Family and domain databases

Gene3Di2.30.30.210. 1 hit.
HAMAPiMF_00754. RNase_P_1.
InterProiIPR002730. RNase_P/MRP_p29.
IPR023538. RNase_P_comp-1.
IPR023534. Rof/RNase_P-like.
[Graphical view]
PfamiPF01868. UPF0086. 1 hit.
[Graphical view]
SMARTiSM00538. POP4. 1 hit.
[Graphical view]
SUPFAMiSSF101744. SSF101744. 1 hit.

Sequencei

Sequence statusi: Complete.

O59425-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MRRNSKERKN RATRRSQGSY QEIIGRTWIF RGAHRGRVTR RNIIWHELIG
60 70 80 90 100
LRVRIVGSTH PAFVGIEGYV IDETRNMLVI AGDRIWKVPK DVCIFEFEAD
110 120
DGTKIKIPGE RLVGRPEMRL KKRWKKW
Length:127
Mass (Da):15,054
Last modified:August 1, 1998 - v1
Checksum:i840FE2A1234FF0AD
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BA000001 Genomic DNA. Translation: BAA30886.1.
PIRiG71186.
RefSeqiNP_143607.1. NC_000961.1.
WP_010885832.1. NC_000961.1.

Genome annotation databases

EnsemblBacteriaiBAA30886; BAA30886; BAA30886.
GeneIDi1442614.
KEGGipho:PH1771.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BA000001 Genomic DNA. Translation: BAA30886.1 .
PIRi G71186.
RefSeqi NP_143607.1. NC_000961.1.
WP_010885832.1. NC_000961.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
1V76 X-ray 2.00 A/B 32-127 [» ]
2ZAE X-ray 2.21 A/C 1-127 [» ]
ProteinModelPortali O59425.
SMRi O59425. Positions 36-127.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

IntActi O59425. 1 interaction.
STRINGi 70601.PH1771.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai BAA30886 ; BAA30886 ; BAA30886 .
GeneIDi 1442614.
KEGGi pho:PH1771.

Phylogenomic databases

eggNOGi COG1588.
HOGENOMi HOG000231353.
KOi K03538.
OMAi ISTKGRV.

Enzyme and pathway databases

BioCyci PHOR70601:GJWR-1769-MONOMER.

Miscellaneous databases

EvolutionaryTracei O59425.

Family and domain databases

Gene3Di 2.30.30.210. 1 hit.
HAMAPi MF_00754. RNase_P_1.
InterProi IPR002730. RNase_P/MRP_p29.
IPR023538. RNase_P_comp-1.
IPR023534. Rof/RNase_P-like.
[Graphical view ]
Pfami PF01868. UPF0086. 1 hit.
[Graphical view ]
SMARTi SM00538. POP4. 1 hit.
[Graphical view ]
SUPFAMi SSF101744. SSF101744. 1 hit.
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 700860 / DSM 12428 / JCM 9974 / NBRC 100139 / OT-3.
  2. "Reconstitution of archaeal ribonuclease P from RNA and four protein components."
    Kouzuma Y., Mizoguchi M., Takagi H., Fukuhara H., Tsukamoto M., Numata T., Kimura M.
    Biochem. Biophys. Res. Commun. 306:666-673(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, BIOPHYSICOCHEMICAL PROPERTIES, SUBUNIT, RNA-BINDING.
    Strain: ATCC 700860 / DSM 12428 / JCM 9974 / NBRC 100139 / OT-3.
  3. "A fifth protein subunit Ph1496p elevates the optimum temperature for the ribonuclease P activity from Pyrococcus horikoshii OT3."
    Fukuhara H., Kifusa M., Watanabe M., Terada A., Honda T., Numata T., Kakuta Y., Kimura M.
    Biochem. Biophys. Res. Commun. 343:956-964(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, BIOPHYSICOCHEMICAL PROPERTIES, SUBUNIT.
    Strain: ATCC 700860 / DSM 12428 / JCM 9974 / NBRC 100139 / OT-3.
  4. "Characterization of the archaeal ribonuclease P proteins from Pyrococcus horikoshii OT3."
    Terada A., Honda T., Fukuhara H., Hada K., Kimura M.
    J. Biochem. 140:293-298(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, SUBUNIT.
    Strain: ATCC 700860 / DSM 12428 / JCM 9974 / NBRC 100139 / OT-3.
  5. "Crystal structure of archaeal ribonuclease P protein Ph1771p from Pyrococcus horikoshii OT3: an archaeal homolog of eukaryotic ribonuclease P protein Rpp29."
    Numata T., Ishimatsu I., Kakuta Y., Tanaka I., Kimura M.
    RNA 10:1423-1432(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (2.0 ANGSTROMS) OF 32-127.
    Strain: ATCC 700860 / DSM 12428 / JCM 9974 / NBRC 100139 / OT-3.
  6. "Structure of an archaeal homolog of the human protein complex Rpp21-Rpp29 that is a key core component for the assembly of active ribonuclease P."
    Honda T., Kakuta Y., Kimura K., Saho J., Kimura M.
    J. Mol. Biol. 384:652-662(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (2.21 ANGSTROMS) IN COMPLEX WITH RNP4, FUNCTION, SUBUNIT, MUTAGENESIS OF 1-MET--ARG-31; GLU-47; GLU-73; ARG-75; LYS-90; PHE-95; ARG-115; 118-MET--TRP-127; LYS-121; 121-LYS-LYS-122; LYS-122 AND 124-TRP--TRP-127.

Entry informationi

Entry nameiRNP1_PYRHO
AccessioniPrimary (citable) accession number: O59425
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 30, 2000
Last sequence update: August 1, 1998
Last modified: November 26, 2014
This is version 90 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3