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Protein

Deglycase PH1704

Gene

PH1704

Organism
Pyrococcus horikoshii (strain ATCC 700860 / DSM 12428 / JCM 9974 / NBRC 100139 / OT-3)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Deglycase that catalyzes the deglycation of the Maillard adducts formed between amino groups of proteins and reactive carbonyl groups of glyoxals. Thus, functions as a protein deglycase that repairs methylglyoxal- and glyoxal-glycated proteins, and releases repaired proteins and lactate or glycolate, respectively. Deglycates cysteine, arginine and lysine residues in proteins, and thus reactivates these proteins by reversing glycation by glyoxals. Acts on early glycation intermediates (hemithioacetals and aminocarbinols), preventing the formation of advanced glycation endproducts (AGE) that cause irreversible damage (By similarity). Also displays proteolytic activity (PubMed:11114201).By similarity1 Publication

Catalytic activityi

An N(omega)-(1-hydroxy-2-oxopropyl)-[protein]-L-arginine + H2O = a [protein]-L-arginine + (R)-lactate.By similarity
An N6-(1-hydroxy-2-oxopropyl)-[protein]-L-lysine + H2O = a [protein]-L-lysine + (R)-lactate.By similarity
An S-(1-hydroxy-2-oxopropyl)-[protein]-L-cysteine + H2O = a [protein]-L-cysteine + (R)-lactate.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei100Nucleophile1 Publication1
Active sitei101PROSITE-ProRule annotation1

GO - Molecular functioni

Keywordsi

Molecular functionHydrolase, Protease

Enzyme and pathway databases

BRENDAi3.4.22.B20. 5244.

Protein family/group databases

MEROPSiC56.001.

Names & Taxonomyi

Protein namesi
Recommended name:
Deglycase PH1704By similarity (EC:3.5.1.124By similarity)
Alternative name(s):
Intracellular protease PH17041 Publication (EC:3.4.22.-1 Publication)
Gene namesi
Ordered Locus Names:PH1704
OrganismiPyrococcus horikoshii (strain ATCC 700860 / DSM 12428 / JCM 9974 / NBRC 100139 / OT-3)
Taxonomic identifieri70601 [NCBI]
Taxonomic lineageiArchaeaEuryarchaeotaThermococciThermococcalesThermococcaceaePyrococcus
Proteomesi
  • UP000000752 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm By similarity

GO - Cellular componenti

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001578271 – 166Deglycase PH1704Add BLAST166

Interactioni

Subunit structurei

Homohexamer formed by a dimer of trimers that assemble into a hollow ring structure.1 Publication

Protein-protein interaction databases

STRINGi70601.PH1704.

Structurei

Secondary structure

1166
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi2 – 6Combined sources5
Helixi13 – 25Combined sources13
Beta strandi29 – 41Combined sources13
Beta strandi47 – 50Combined sources4
Helixi54 – 56Combined sources3
Helixi59 – 61Combined sources3
Beta strandi63 – 67Combined sources5
Helixi72 – 76Combined sources5
Helixi80 – 91Combined sources12
Beta strandi96 – 99Combined sources4
Turni100 – 103Combined sources4
Helixi104 – 109Combined sources6
Helixi121 – 123Combined sources3
Helixi124 – 129Combined sources6
Beta strandi140 – 142Combined sources3
Beta strandi145 – 148Combined sources4
Helixi151 – 153Combined sources3
Helixi154 – 165Combined sources12

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1G2IX-ray2.00A/B/C1-166[»]
ProteinModelPortaliO59413.
SMRiO59413.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiO59413.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini1 – 166PfpI endopeptidasePROSITE-ProRule annotationAdd BLAST166

Sequence similaritiesi

Belongs to the peptidase C56 family.Curated

Phylogenomic databases

eggNOGiarCOG00769. Archaea.
COG0693. LUCA.
HOGENOMiHOG000063195.
KOiK05520.
OMAiMVGHTVH.
OrthoDBiPOG093Z0CI8.

Family and domain databases

Gene3Di3.40.50.880. 1 hit.
InterProiView protein in InterPro
IPR029062. Class_I_gatase-like.
IPR002818. DJ-1/PfpI.
IPR006286. Peptidase_C56.
PfamiView protein in Pfam
PF01965. DJ-1_PfpI. 1 hit.
SUPFAMiSSF52317. SSF52317. 1 hit.
TIGRFAMsiTIGR01382. PfpI. 1 hit.
PROSITEiView protein in PROSITE
PS51276. PEPTIDASE_C56_PFPI. 1 hit.

Sequencei

Sequence statusi: Complete.

O59413-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKVLFLTANE FEDVELIYPY HRLKEEGHEV YIASFERGTI TGKHGYSVKV
60 70 80 90 100
DLTFDKVNPE EFDALVLPGG RAPERVRLNE KAVSIARKMF SEGKPVASIC
110 120 130 140 150
HGPQILISAG VLRGRKGTSY PGIKDDMINA GVEWVDAEVV VDGNWVSSRV
160
PADLYAWMRE FVKLLK
Length:166
Mass (Da):18,624
Last modified:August 1, 1998 - v1
Checksum:iCED046ED1789808C
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BA000001 Genomic DNA. Translation: BAA30818.1.
PIRiC71178.
RefSeqiWP_010885770.1. NC_000961.1.

Genome annotation databases

EnsemblBacteriaiBAA30818; BAA30818; BAA30818.
GeneIDi1442551.
KEGGipho:PH1704.

Similar proteinsi

Entry informationi

Entry nameiDEGLY_PYRHO
AccessioniPrimary (citable) accession number: O59413
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 15, 1999
Last sequence update: August 1, 1998
Last modified: September 27, 2017
This is version 107 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. Peptidase families
    Classification of peptidase families and list of entries
  3. SIMILARITY comments
    Index of protein domains and families