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Protein

S-adenosyl-L-methionine-dependent tRNA 4-demethylwyosine synthase

Gene

taw1

Organism
Pyrococcus horikoshii (strain ATCC 700860 / DSM 12428 / JCM 9974 / NBRC 100139 / OT-3)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Component of the wyosine derivatives biosynthesis pathway that catalyzes the condensation of N-methylguanine with 2 carbon atoms from pyruvate to form the tricyclic 4-demethylwyosine (imG-14) on guanosine-37 of tRNA(Phe).UniRule annotation

Catalytic activityi

N(1)-methylguanine(37) in tRNA(Phe) + pyruvate + S-adenosyl-L-methionine = 4-demethylwyosine(37) in tRNA(Phe) + L-methionine + 5'-deoxyadenosine + CO2 + H2O.UniRule annotation

Cofactori

Protein has several cofactor binding sites:

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi45Iron-sulfur 1 (2Fe-2S)Curated1
Metal bindingi58Iron-sulfur 1 (2Fe-2S)Curated1
Metal bindingi71Iron-sulfur 1 (2Fe-2S)Curated1
Metal bindingi81Iron-sulfur 2 (4Fe-4S-S-AdoMet)Curated1
Metal bindingi85Iron-sulfur 2 (4Fe-4S-S-AdoMet)Curated1
Metal bindingi88Iron-sulfur 2 (4Fe-4S-S-AdoMet)Curated1

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Lyase

Keywords - Biological processi

tRNA processing

Keywords - Ligandi

2Fe-2S, 4Fe-4S, Iron, Iron-sulfur, Metal-binding, S-adenosyl-L-methionine

Enzyme and pathway databases

BioCyciMetaCyc:MONOMER-18047.
BRENDAi4.1.3.44. 5244.

Names & Taxonomyi

Protein namesi
Recommended name:
S-adenosyl-L-methionine-dependent tRNA 4-demethylwyosine synthaseUniRule annotation (EC:4.1.3.44UniRule annotation)
Alternative name(s):
tRNA wyosine derivatives biosynthesis protein Taw1UniRule annotation
Gene namesi
Name:taw1UniRule annotation
Ordered Locus Names:PH1705
OrganismiPyrococcus horikoshii (strain ATCC 700860 / DSM 12428 / JCM 9974 / NBRC 100139 / OT-3)
Taxonomic identifieri70601 [NCBI]
Taxonomic lineageiArchaeaEuryarchaeotaThermococciThermococcalesThermococcaceaePyrococcus
Proteomesi
  • UP000000752 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00004078571 – 342S-adenosyl-L-methionine-dependent tRNA 4-demethylwyosine synthaseAdd BLAST342

Interactioni

Subunit structurei

Monomer.UniRule annotation1 Publication

Protein-protein interaction databases

IntActiO59412. 1 interactor.
MINTiMINT-1502553.
STRINGi70601.PH1705.

Structurei

Secondary structure

1342
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi22 – 30Combined sources9
Beta strandi34 – 37Combined sources4
Beta strandi40 – 42Combined sources3
Helixi48 – 54Combined sources7
Helixi59 – 64Combined sources6
Helixi68 – 70Combined sources3
Beta strandi71 – 76Combined sources6
Beta strandi91 – 94Combined sources4
Helixi107 – 122Combined sources16
Helixi133 – 139Combined sources7
Beta strandi144 – 147Combined sources4
Beta strandi149 – 151Combined sources3
Helixi153 – 155Combined sources3
Helixi159 – 168Combined sources10
Beta strandi172 – 177Combined sources6
Helixi182 – 190Combined sources9
Beta strandi196 – 202Combined sources7
Helixi207 – 214Combined sources8
Beta strandi216 – 219Combined sources4
Helixi221 – 232Combined sources12
Beta strandi236 – 245Combined sources10
Turni247 – 249Combined sources3
Helixi254 – 264Combined sources11
Beta strandi267 – 273Combined sources7
Helixi286 – 288Combined sources3
Helixi292 – 303Combined sources12
Beta strandi309 – 315Combined sources7
Helixi316 – 318Combined sources3
Beta strandi320 – 325Combined sources6
Beta strandi328 – 333Combined sources6
Helixi334 – 336Combined sources3
Beta strandi337 – 339Combined sources3

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2YX0X-ray2.21A1-342[»]
ProteinModelPortaliO59412.
SMRiO59412.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiO59412.

Family & Domainsi

Sequence similaritiesi

Belongs to the TYW1 family.UniRule annotation

Phylogenomic databases

eggNOGiarCOG04174. Archaea.
COG0731. LUCA.
HOGENOMiHOG000224906.
KOiK15449.
OMAiHRCLQMT.

Family and domain databases

Gene3Di3.20.20.70. 1 hit.
HAMAPiMF_01921. TYW1_archaea. 1 hit.
InterProiIPR013785. Aldolase_TIM.
IPR007197. rSAM.
IPR013917. tRNA_wybutosine-synth.
IPR023993. TYW1_archaea.
[Graphical view]
PfamiPF04055. Radical_SAM. 1 hit.
PF08608. Wyosine_form. 1 hit.
[Graphical view]
TIGRFAMsiTIGR03972. rSAM_TYW1. 1 hit.

Sequencei

Sequence statusi: Complete.

O59412-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MMEMITIKPG KITVQANPNM PKEVAELFRK QHYEIVGRHS GVKLCHWLKK
60 70 80 90 100
SLTEGRFCYK QKFYGIHSHR CLQMTPVLAW CTHNCIFCWR PMENFLGTEL
110 120 130 140 150
PQPWDDPAFI VEESIKAQRK LLIGYKGNPK VDKKKFEEAW NPTHAAISLS
160 170 180 190 200
GEPMLYPYMG DLVEEFHKRG FTTFIVTNGT IPERLEEMIK EDKLPTQLYV
210 220 230 240 250
SITAPDIETY NSVNIPMIPD GWERILRFLE LMRDLPTRTV VRLTLVKGEN
260 270 280 290 300
MHSPEKYAKL ILKARPMFVE AKAYMFVGYS RNRLTINNMP SHQDIREFAE
310 320 330 340
ALVKHLPGYH IEDEYEPSRV VLIMRDDVDP QGTGVEGRFI KH
Length:342
Mass (Da):39,840
Last modified:August 1, 1998 - v1
Checksum:iF321AF462AA262D7
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BA000001 Genomic DNA. Translation: BAA30819.1.
PIRiD71178.

Genome annotation databases

EnsemblBacteriaiBAA30819; BAA30819; BAA30819.
KEGGipho:PH1705.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BA000001 Genomic DNA. Translation: BAA30819.1.
PIRiD71178.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2YX0X-ray2.21A1-342[»]
ProteinModelPortaliO59412.
SMRiO59412.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiO59412. 1 interactor.
MINTiMINT-1502553.
STRINGi70601.PH1705.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiBAA30819; BAA30819; BAA30819.
KEGGipho:PH1705.

Phylogenomic databases

eggNOGiarCOG04174. Archaea.
COG0731. LUCA.
HOGENOMiHOG000224906.
KOiK15449.
OMAiHRCLQMT.

Enzyme and pathway databases

BioCyciMetaCyc:MONOMER-18047.
BRENDAi4.1.3.44. 5244.

Miscellaneous databases

EvolutionaryTraceiO59412.

Family and domain databases

Gene3Di3.20.20.70. 1 hit.
HAMAPiMF_01921. TYW1_archaea. 1 hit.
InterProiIPR013785. Aldolase_TIM.
IPR007197. rSAM.
IPR013917. tRNA_wybutosine-synth.
IPR023993. TYW1_archaea.
[Graphical view]
PfamiPF04055. Radical_SAM. 1 hit.
PF08608. Wyosine_form. 1 hit.
[Graphical view]
TIGRFAMsiTIGR03972. rSAM_TYW1. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiTYW1_PYRHO
AccessioniPrimary (citable) accession number: O59412
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 3, 2011
Last sequence update: August 1, 1998
Last modified: November 2, 2016
This is version 96 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.