O59402 (LYSK_PYRHO) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Acetyl-lysine deacetylase
EC=3.5.1.-

Gene names

Name:	lysK
Ordered Locus Names:	PH1715

Organism

Pyrococcus horikoshii (strain ATCC 700860 / DSM 12428 / JCM 9974 / NBRC 100139 / OT-3) [Complete proteome] [HAMAP]

Taxonomic identifier

70601 [NCBI]

Taxonomic lineage

Archaea › Euryarchaeota › Thermococci › Thermococcales › Thermococcaceae › Pyrococcus

Protein attributes

Sequence length	325 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	N(2)-acetyl-L-lysine + H2O = acetate + L-lysine. HAMAP MF_01120
Cofactor	Binds 2 zinc or cobalt ions per subunit By similarity. HAMAP MF_01120 Glutathione By similarity. HAMAP MF_01120
Pathway	Amino-acid biosynthesis; L-lysine biosynthesis via AAA pathway; L-lysine from L-alpha-aminoadipate (Thermus route): step 5/5. HAMAP MF_01120
Subunit structure	Homodimer By similarity. HAMAP MF_01120
Subcellular location	Cytoplasm Probable HAMAP MF_01120.
Sequence similarities	Belongs to the peptidase M20A family. LysK subfamily.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Lysine biosynthesis
Cellular component	Cytoplasm
Ligand	Cobalt Metal-binding Zinc
Molecular function	Hydrolase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	lysine biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW proteolysis Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	cobalt ion binding Inferred from electronic annotation. Source: InterPro hydrolase activity, acting on carbon-nitrogen (but not peptide) bonds, in linear amides Inferred from electronic annotation. Source: InterPro metallopeptidase activity Inferred from electronic annotation. Source: InterPro zinc ion binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 325	325	Acetyl-lysine deacetylase HAMAP MF_01120		PRO_0000185348
Sites
	Active site	68	1	By similarity
	Active site	117	1	Proton acceptor By similarity
	Metal binding	66	1	Cobalt or zinc 1 By similarity
	Metal binding	90	1	Cobalt or zinc 1 By similarity
	Metal binding	90	1	Cobalt or zinc 2 By similarity
	Metal binding	118	1	Cobalt or zinc 2 By similarity
	Metal binding	139	1	Cobalt or zinc 1 By similarity
	Metal binding	297	1	Cobalt or zinc 2 By similarity

Sequences

	Sequence		Length	Mass (Da)
	O59402 [UniParc]. Last modified August 1, 1998. Version 1. Checksum: FF118F554983A5C4	FASTA	325	36,084
	10 20 30 40 50 60 MISEEEKIKF LKELVEIYSP TGREEEAAKF IKEKFEEYGI EAYVDNVGNV IARKSGEGPL 70 80 90 100 110 120 VLLAGHIDTV PGYIPVRIEG EVLWGRGSVD AKGPLATLFF STIEGNANVI FAGLVDEEGF 130 140 150 160 170 180 SKGARNLKIP RPDYIIVGEP SGTNGVTIGY KGSLTVRFVE RVEKVHSSLG VGAAERLIEK 190 200 210 220 230 240 WLEISKDFSD GFNGLNGRIV RFLAYDREFE FYGEMIINLR TPPGYVPPLE WDIIDFVPAY 250 260 270 280 290 300 EVDRRSPLVR AFVKSIREAG LKPKLKKKSG TADTNILGPK FGVDAVAYGP GDSKLDHTPY 310 320 ERINLREYLK SIEILKAVLR KLKGG

References

[1]

"Complete sequence and gene organization of the genome of a hyper-thermophilic archaebacterium, Pyrococcus horikoshii OT3." Kawarabayasi Y., Sawada M., Horikawa H., Haikawa Y., Hino Y., Yamamoto S., Sekine M., Baba S., Kosugi H., Hosoyama A., Nagai Y., Sakai M., Ogura K., Otsuka R., Nakazawa H., Takamiya M., Ohfuku Y., Funahashi T. , Tanaka T., Kudoh Y., Yamazaki J., Kushida N., Oguchi A., Aoki K., Yoshizawa T., Nakamura Y., Robb F.T., Horikoshi K., Masuchi Y., Shizuya H., Kikuchi H. DNA Res. 5:55-76(1998) [PubMed: 9679194] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 700860 / DSM 12428 / JCM 9974 / NBRC 100139 / OT-3.

Cross-references

Sequence databases
EMBL GenBank DDBJ	BA000001 Genomic DNA. Translation: BAA30829.1.
PIR	F71179.
RefSeq	NP_143558.1. NC_000961.1.
3D structure databases
ProteinModelPortal	O59402.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	EBPYRT00000000378; EBPYRP00000000378; EBPYRG00000000378.
GeneID	1442562.
GenomeReviews	Gene locus PH1715 in contig BA000001_GR.
KEGG	pho:PH1715.
NMPDR	fig|70601.1.peg.1676.
Organism-specific databases
CMR	Search...
Phylogenomic databases
GeneTree	EBGT00050000022564.
HOGENOM	HBG728841.
OMA	LDHTPYE.
ProtClustDB	PRK04443.
Enzyme and pathway databases
BioCyc	PHOR70601:PH1715-MONOMER.
Family and domain databases
HAMAP	MF_01120. Acet-lys_deacetyl. [Tree]
InterPro	IPR023556. Acetyl-lysine_deAcylase. IPR001261. ArgE/DapE_CS. IPR010175. DapE_lys_deAc. IPR002933. Peptidase_M20. [Graphical view]
KO	K05831.
Pfam	PF01546. Peptidase_M20. 1 hit. [Graphical view]
TIGRFAMs	TIGR01902. DapE-lys-deAc. 1 hit.
PROSITE	PS00758. ARGE_DAPE_CPG2_1. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

LYSK_PYRHO

Accession

Primary (citable) accession number: O59402

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 16, 2004
Last sequence update:	August 1, 1998
Last modified:	November 16, 2011
This is version 70 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program